Measurement of Isothermal Pressure of Lattice Gas by Random Walk

We present a computational random walk method of measuring the isothermal pressure of the lattice gas with and without the excluded volume interaction. The method is based on the discretization of the exact thermodynamic relation for the pressure. The simulation results are in excellent agreement with the theoretical predictions.Comment: 10 Pages, 2 Figures, Teaching Material. To Appear in Physica

Low Catalyst Loadings in Olefin Metathesis: Synthesis of Nitrogen Heterocycles by Ring-Closing Metathesis

A series of ruthenium catalysts have been screened under ring-closing metathesis (RCM) conditions to produce five-, six-, and seven-membered carbamate-protected cyclic amines. Many of these catalysts demonstrated excellent RCM activity and yields with as low as 500 ppm catalyst loadings. RCM of the five-membered carbamate series could be run neat, the six-membered carbamate series could be run at 1.0 M, and the seven-membered carbamate series worked best at 0.2−0.05 M

Identifying the dominant personality profiles in medical students: Implications for their well-being and resilience

Purpos

ALMA Observation of NGC5135: The Circumnuclear CO(6-5) and Dust Continuum Emission at 45 Parsec Resolution[⋆\star]

We present high-resolution (0.17\arcsec $\times$ 0.14\arcsec) Atacama Large Millimeter/submillimeter Array (ALMA) observations of the CO\,(6-5) line, and 435\um\ dust continuum emission within a $\sim$9\arcsec $\times$ 9\arcsec\ area centered on the nucleus of the galaxy NGC\,5135. NGC\,5135 is a well-studied luminous infrared galaxy that also harbors a Compton-thick active galactic nucleus (AGN). At the achieved resolution of 48 $\times$ 40\,pc, the CO\,(6-5) and dust emissions are resolved into gas "clumps" along the symmetrical dust lanes associated with the inner stellar bar. The clumps have radii between $\sim$45-180\,pc and CO\,(6-5) line widths of $\sim$60-88\,\kms. The CO\,(6-5) to dust continuum flux ratios vary among the clumps and show an increasing trend with the \FeII/Br-$\gamma$ ratios, which we interpret as evidence for supernova-driven shocked gas providing a significant contribution to the \co65\ emission. The central AGN is undetected in continuum, nor in CO\,(6-5) if its line velocity width is no less than $\sim$\,40\,\kms. We estimate that the AGN contributes at most 1\% of the integrated CO\,(6-5) flux of 512 $\pm$ 24$\,$Jy\kms\ within the ALMA field of view, which in turn accounts for $\sim$32\% of the CO\,(6-5) flux of the whole galaxy.Comment: 21 pages, 12 figures, Accepted for publication in Ap

PERIOD–TIMELESS Interval Timer May Require an Additional Feedback Loop

In this study we present a detailed, mechanism-based mathematical framework of Drosophila circadian rhythms. This framework facilitates a more systematic approach to understanding circadian rhythms using a comprehensive representation of the network underlying this phenomenon. The possible mechanisms underlying the cytoplasmic “interval timer” created by PERIOD–TIMELESS association are investigated, suggesting a novel positive feedback regulatory structure. Incorporation of this additional feedback into a full circadian model produced results that are consistent with previous experimental observations of wild-type protein profiles and numerous mutant phenotypes

The Global Lake Ecological Observatory Network (GLEON): the evolution of grassroots network science

Nine years later, with over 380 members from 40 countries, and 50 publications to its credit, GLEON is growing at a rapid pace and pushing the boundaries of the practice of network science. GLEON is really three networks: a network of lakes, data, and peopl

Structural insights on TRPV5 gating by endogenous modulators.

TRPV5 is a transient receptor potential channel involved in calcium reabsorption. Here we investigate the interaction of two endogenous modulators with TRPV5. Both phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and calmodulin (CaM) have been shown to directly bind to TRPV5 and activate or inactivate the channel, respectively. Using cryo-electron microscopy (cryo-EM), we determined TRPV5 structures in the presence of dioctanoyl PI(4,5)P2 and CaM. The PI(4,5)P2 structure reveals a binding site between the N-linker, S4-S5 linker and S6 helix of TRPV5. These interactions with PI(4,5)P2 induce conformational rearrangements in the lower gate, opening the channel. The CaM structure reveals two TRPV5 C-terminal peptides anchoring a single CaM molecule and that calcium inhibition is mediated through a cation-π interaction between Lys116 on the C-lobe of calcium-activated CaM and Trp583 at the intracellular gate of TRPV5. Overall, this investigation provides insight into the endogenous modulation of TRPV5, which has the potential to guide drug discovery

Microbial Community Structure of Leaf-Cutter Ant Fungus Gardens and Refuse Dumps

BACKGROUND: Leaf-cutter ants use fresh plant material to grow a mutualistic fungus that serves as the ants' primary food source. Within fungus gardens, various plant compounds are metabolized and transformed into nutrients suitable for ant consumption. This symbiotic association produces a large amount of refuse consisting primarily of partly degraded plant material. A leaf-cutter ant colony is thus divided into two spatially and chemically distinct environments that together represent a plant biomass degradation gradient. Little is known about the microbial community structure in gardens and dumps or variation between lab and field colonies. METHODOLOGY/PRINCIPAL FINDINGS: Using microbial membrane lipid analysis and a variety of community metrics, we assessed and compared the microbiota of fungus gardens and refuse dumps from both laboratory-maintained and field-collected colonies. We found that gardens contained a diverse and consistent community of microbes, dominated by Gram-negative bacteria, particularly gamma-Proteobacteria and Bacteroidetes. These findings were consistent across lab and field gardens, as well as host ant taxa. In contrast, dumps were enriched for Gram-positive and anaerobic bacteria. Broad-scale clustering analyses revealed that community relatedness between samples reflected system component (gardens/dumps) rather than colony source (lab/field). At finer scales samples clustered according to colony source. CONCLUSIONS/SIGNIFICANCE: Here we report the first comparative analysis of the microbiota from leaf-cutter ant colonies. Our work reveals the presence of two distinct communities: one in the fungus garden and the other in the refuse dump. Though we find some effect of colony source on community structure, our data indicate the presence of consistently associated microbes within gardens and dumps. Substrate composition and system component appear to be the most important factor in structuring the microbial communities. These results thus suggest that resident communities are shaped by the plant degradation gradient created by ant behavior, specifically their fungiculture and waste management