Konzipierung und Implementierung einer Online-Hilfe für ein virtuelles Konferenzsystem im Rahmen des von der Europäischen Kommission geförderten Projektes "Invite EU"

Die vorliegende Arbeit beschäftigt sich mit der Konzipierung und Einführung einer Online-Hilfe für ein virtuelles Konferenzsystem, das im Rahmen des von der Europäischen Kommission geförderten Projektes "Invite" beim Fraunhofer Institut für Arbeitswirtschaft und Organisation im Bereich Softwaretechnik entwickelt wird. Der erste Teil der Arbeit untersucht die theoretischen Aspekte der Online- Dokumentation und Online-Hilfe. Nach der Erstellung eines Projektplanes, in dem die Informationsplanung, die Spezifizierung der Inhalte, die Implementierung, die Produktion und die Evaluation dokumentiert werden, folgt im letzten Teil ein Ausblick mit den neuen Aspekten, die sich durch die Arbeit ergeben haben. Der praktische Teil der Arbeit, die Erstellung der Online-Hilfe, wurde beim Fraunhofer Institut durchgeführt und liegt der Arbeit auf CD-ROM bei

Hörqualität im Vergleich - Subjektive Beurteilung der Hörqualität von Kindern mit Cochleaimplantaten und Hörgeräten im Alter zwischen 8 und 17 Jahren

Nachdem das Forschungsgebiet der Selbsteinschätzung von Hörqualität bei Kindern und Jugendlichen im schulischen Alltag bislang kaum betrachtet wurde, setzt diese Studienarbeit an dieser Stelle an. Im Rahmen der Studie sollte bewiesen werden, dass es keine Unterschiede in der Hörqualität durch Selbsteinschätzung wie auch in der Hörqualität durch Fremdeinschätzung zwischen Cochleaimplantat- und Hörgeräteträgern gibt. Zudem sollte ermittelt werden, wie hoch die Hörqualität der einzelnen Versorgungsgruppen in Selbst- und Fremdeinschätzung ist. Alle Daten wurden aus einer Gruppe von 21 Cochleaimplantatträgern (m = 9; w = 12) und einer Gruppe von 50 Hörgeräteträgern (m = 28; w = 22) erhoben. Die mittlere Altersgruppe der Cochleaimplantatträger lag bei „13 - 15 Jahren“, die der Hörgeräteträger „10 - 12 Jahren“. Im Durchschnitt waren alle Kinder seit mehr als 3 Jahren bilateral mit Hörsystemen versorgt. Getestet wurde mittels neu entwickelten Fragebögen, bewertet anhand eines speziell dafür ausgelegten Punktesystems. Die Hörqualität der Selbsteinschätzung konnte für beide Versorgungsgruppen mit „sehr gut“ bewertet werden. Die Ergebnisse der Hörqualität für die Fremdeinschätzung war für beide Gruppen etwas schlechter und wurde als „gut“ eingestuft. Der größte Unterschied bestand in der Hörqualität der Selbst- und Fremdeinschätzung zusammen. Hier konnten die Cochleaimplantatträger eine „gute“, die Hörgeräteträger jedoch eine „sehr gute“ Hörqualität erreichen. Kinder und Jugendliche mit Cochleaimplantaten schätzen ihre Hörfähigkeit in allen Bereichen der Hörqualität, sowohl in ihrer Selbst- als auch in ihrer Fremdeinschätzung schlechter ein, als die Kinder und Jugendlichen mit Hörgeräten

Diversity Management and the Political Economy of Policing

Diversity management and diversity training have been part of the standard management repertoire for several decades, and have recently received fresh impetus in the UK through the Equality Act 2010. The Police Services in England and Wales and in Scotland have further reasons to ensure the fair treatment of their own workforces and equality in their dealings with the public since the Macpherson Inquiry and the subsequent revelations relating to the Stephen Lawrence case. For the Police Service, diversity is particularly crucial as it forms a key element of public legitimacy and therefore impacts upon the very principle of ‘policing by consent’, the foundation of British policing (Jackson et al. 2012). However, diversity policies and diversity training tend to be viewed narrowly and used as a decontextualised medium to reduce racism (and other ‘isms’), seen as fulfilling their purpose regardless of the political and occupational context. This thesis, in contrast, suggests that there is a need to examine diversity management and diversity training, not only within an organisational context, but also within the broader political economy into which it is introduced and in which it is implemented. Tracing the various aspects that make up the political economy of policing, the thesis outlines social, economic, legal and political influences, as well as the occupational culture of the police and its emotional ecology. Given the longitudinal design of the research, and the profound changes that have occurred to the political economy of policing over a relatively short time, the thesis is able to examine the impact of these changes on diversity practices within the Police Service of Scotland. Longitudinal data collected at two points in time, 2008/9 and 2013 – straddling not only the introduction of the Equality Act 2010, but also the creation of a single Police Service in Scotland, amongst other changes – suggests that significant changes have occurred to diversity training and diversity professionals, as well as to the ways in which diversity is managed. Using the notion of emotional spaces, diversity training in particular reveals complex interactions in the context of the changes, exposing the tensions police officers and police staff are currently experiencing. Drawing on the analytical framework of emotional ecology, it is argued that in addition to other changes to the political economy of policing, diversity training courses reflect demands for the police to be more open, sensitive and collaborative, by challenging and ‘opening up’ the emotional ecology of the police during training. Interviews and longitudinal observational data suggest that this process has intensified greatly since the creation of Police Scotland, thereby placing competing demands on officers to consolidate the new with the conventional emotional ecology of the police

Global DNA hypomethylation prevents consolidation of differentiation programs and allows reversion to the embryonic stem cell state.

DNA methylation patterns change dynamically during mammalian development and lineage specification, yet scarce information is available about how DNA methylation affects gene expression profiles upon differentiation. Here we determine genome-wide transcription profiles during undirected differentiation of severely hypomethylated (Dnmt1⁻/⁻) embryonic stem cells (ESCs) as well as ESCs completely devoid of DNA methylation (Dnmt1⁻/⁻;Dnmt3a⁻/⁻;Dnmt3b⁻/⁻ or TKO) and assay their potential to transit in and out of the ESC state. We find that the expression of only few genes mainly associated with germ line function and the X chromosome is affected in undifferentiated TKO ESCs. Upon initial differentiation as embryoid bodies (EBs) wild type, Dnmt1⁻/⁻ and TKO cells downregulate pluripotency associated genes and upregulate lineage specific genes, but their transcription profiles progressively diverge upon prolonged EB culture. While Oct4 protein levels are completely and homogeneously suppressed, transcription of Oct4 and Nanog is not completely silenced even at late stages in both Dnmt1⁻/⁻ and TKO EBs. Despite late wild type and Dnmt1⁻/⁻ EBs showing a much higher degree of concordant expression, after EB dissociation and replating under pluripotency promoting conditions both Dnmt1⁻/⁻ and TKO cells, but not wild type cells rapidly revert to expression profiles typical of undifferentiated ESCs. Thus, while DNA methylation seems not to be critical for initial activation of differentiation programs, it is crucial for permanent restriction of developmental fate during differentiation

In Situ Mass Spectrometric and Kinetic Investigations of Soai's Asymmetric Autocatalysis

Chemical reactions that lead to a spontaneous symmetry breaking or amplification of the enantiomeric excess are of fundamental interest in explaining the formation of a homochiral world. An outstanding example is Soai's asymmetric autocatalysis, in which small enantiomeric excesses of the added product alcohol are amplified in the reaction of diisopropylzinc and pyrimidine‐5‐carbaldehydes. The exact mechanism is still in dispute due to complex reaction equilibria and elusive intermediates. In situ high‐resolution mass spectrometric measurements, detailed kinetic analyses and doping with in situ reacting reaction mixtures show the transient formation of hemiacetal complexes, which can establish an autocatalytic cycle. We propose a mechanism that explains the autocatalytic amplification involving these hemiacetal complexes. Comprehensive kinetic experiments and modelling of the hemiacetal formation and the Soai reaction allow the precise prediction of the reaction progress, the enantiomeric excess as well as the enantiomeric excess dependent time shift in the induction period. Experimental structural data give insights into the privileged properties of the pyrimidyl units and the formation of diastereomeric structures leading to an efficient amplification of even minimal enantiomeric excesses, respectively

Determinants of RNA metabolism in the Schizosaccharomyces pombe genome

To decrypt the regulatory code of the genome, sequence elements must be defined that determine the kinetics of RNA metabolism and thus gene expression. Here, we attempt such decryption in an eukaryotic model organism, the fission yeast S. pombe. We first derive an improved genome annotation that redefines borders of 36% of expressed mRNAs and adds 487 non-coding RNAs (ncRNAs). We then combine RNA labeling invivo with mathematical modeling to obtain rates of RNA synthesis and degradation for 5,484 expressed RNAs and splicing rates for4,958 introns. We identify functional sequence elements inDNA and RNA that control RNA metabolic rates and quantifythecontributions of individual nucleotides to RNA synthesis,splicing, and degradation. Our approach reveals distinct kineticsof mRNA and ncRNA metabolism, separates antisense regulation by transcription interference from RNA interference, and provides a general tool for studying the regulatory code of genomes

Non‐invasive and tracheostomy invasive ventilation in amyotrophic lateral sclerosis: Utilization and survival rates in a cohort study over 12 years in Germany

Background and purpose: The aim of this study was to investigate utilization rates, treatment pathways and survival prognosis in patients with amyotrophic lateral sclerosis (ALS) undergoing non-invasive (NIV) and tracheostomy invasive ventilation (TIV) in a real-world setting. Methods: A prospective cohort study using a single-centre register of 2702 ALS patients (2007 to 2019) was conducted. Utilization of NIV/TIV and survival data were analysed in three cohorts: (i) non-NIV; (ii) NIV (NIV without subsequent TIV); and (iii) TIV (including TIV preceded by NIV). Results: A total of 1720 patients with available data were identified, 72.0% of whom (n = 1238) did not receive ventilation therapy. NIV was performed in 20.8% of patients (n = 358). TIV was performed in 9.5% of patients (n = 164), encompassing both primary TIV (7.2%, n = 124) and TIV with preceding NIV (2.3%, n = 40). TIV was more often utilized without previous NIV (25.7% vs. 8.3% of all ventilated patients), demonstrating that primary TIV was the prevailing pathway for invasive ventilation. The median (range) survival was significantly longer in the NIV cohort (40.8 [37.2–44.3] months) and the TIV cohort (82.1 [68.7–95.6] months) as compared to the non-NIV cohort (33.6 [31.6–35.7] months). Conclusions: Although NIV represents the standard of care, its utilization rate was low. TIV was mainly started without preceding NIV, suggesting that TIV may not be confined to NIV treatment escalation. However, TIV was pursued in a minority of patients who had previously undergone NIV. The survival benefit observed in the patients with NIV was equal to that reported in a controlled pivotal trial, but the prognosis with TIV is highly variable. The determinants of utilization of NIV/TIV and of survival (bulbar syndrome, availability of ventilation-related home nursing, cultural factors) warrant further investigation

CG-SENSE revisited: Results from the first ISMRM reproducibility challenge

Purpose: The aim of this work is to shed light on the issue of reproducibility in MR image reconstruction in the context of a challenge. Participants had to recreate the results of "Advances in sensitivity encoding with arbitrary k-space trajectories" by Pruessmann et al. Methods: The task of the challenge was to reconstruct radially acquired multi-coil k-space data (brain/heart) following the method in the original paper, reproducing its key figures. Results were compared to consolidated reference implementations created after the challenge, accounting for the two most common programming languages used in the submissions (Matlab/Python). Results: Visually, differences between submissions were small. Pixel-wise differences originated from image orientation, assumed field-of-view or resolution. The reference implementations were in good agreement, both visually and in terms of image similarity metrics. Discussion and Conclusion: While the description level of the published algorithm enabled participants to reproduce CG-SENSE in general, details of the implementation varied, e.g., density compensation or Tikhonov regularization. Implicit assumptions about the data lead to further differences, emphasizing the importance of sufficient meta-data accompanying open data sets. Defining reproducibility quantitatively turned out to be non-trivial for this image reconstruction challenge, in the absence of ground-truth results. Typical similarity measures like NMSE of SSIM were misled by image intensity scaling and outlier pixels. Thus, to facilitate reproducibility, researchers are encouraged to publish code and data alongside the original paper. Future methodological papers on MR image reconstruction might benefit from the consolidated reference implementations of CG-SENSE presented here, as a benchmark for methods comparison.Comment: Submitted to Magnetic Resonance in Medicine; 29 pages with 10 figures and 1 tabl

Calcium-Mediated Actin Reset (Caar) Mediates Acute Cell Adaptations

Actin has well established functions in cellular morphogenesis. However, it is not well understood how the various actin assemblies in a cell are kept in a dynamic equilibrium, in particular when cells have to respond to acute signals. Here, we characterize a rapid and transient actin reset in response to increased intracellular calcium levels. Within seconds of calcium influx, the formin INF2 stimulates filament polymerization at the endoplasmic reticulum (ER), while cortical actin is disassembled. The reaction is then reversed within a few minutes. This Calcium-mediated actin reset (CaAR) occurs in a wide range of mammalian cell types and in response to many physiological cues. CaAR leads to transient immobilization of organelles, drives reorganization of actin during cell cortex repair, cell spreading and wound healing, and induces long-lasting changes in gene expression. Our findings suggest that CaAR acts as fundamental facilitator of cellular adaptations in response to acute signals and stress