Can Autonomous Art Change the World?

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Clinical and molecular features of methicillin-resistant,coagulase-negative staphylococci of pets and horses

Objectives To determine the antibiotic resistance and fingerprint profiles of methicillin-resistant coagulase-negative staphylococci (MRCoNS) from animal infections among different practices and examine the history of antibiotic treatment. Methods Isolates were identified by mass spectrometry and tested for antimicrobial resistance by broth dilution, microarrays and sequence analysis of the topoisomerases. Diversity was assessed by PFGE, icaA PCR and staphylococcal cassette chromosome mec (SCCmec), arginine catabolic mobile element (ACME) and multilocus sequence typing. Clinical records were examined retrospectively. Results MRCoNS were identified as Staphylococcus epidermidis (n = 20), Staphylococcus haemolyticus (n = 17), Staphylococcus hominis (n = 3), Staphylococcus capitis (n = 1), Staphylococcus cohnii (n = 1) and Staphylococcus warneri (n = 1). PFGE identified one clonal lineage in S. hominis isolates and several in S. haemolyticus and S. epidermidis. Fourteen sequence types were identified in S. epidermidis, with sequence type 2 (ST2) and ST5 being predominant. Ten isolates contained SCCmec IV, seven contained SCCmec V and the others were non-typeable. ACMEs were detected in 11 S. epidermidis isolates. One S. hominis and 10 S. epidermidis isolates were icaA positive. In addition to mecA-mediated β-lactam resistance, the most frequent resistance was to gentamicin/kanamycin [aac(6′)-Ie-aph(2′)-Ia, aph(3′)-III] (n = 34), macrolides/lincosamides [erm(C), erm(A), msr, lnu(A)] (n = 31), tetracycline [tet(K)] (n = 22), streptomycin [str, ant(6)-Ia] (n = 20), trimethoprim [dfr(A), dfr(G)] (n = 17), sulfamethoxazole (n = 34) and fluoroquinolones [amino acid substitutions in GyrA and GrlA] (n = 30). Clinical data suggest selection through multiple antibiotic courses and emphasize the importance of accurate diagnosis and antibiograms. Conclusions MRCoNS from animal infection sites are genetically heterogeneous multidrug-resistant strains that represent a new challenge in the prevention and therapy of infections in veterinary clinic

Molecular quantum spin network controlled by a single qubit

Scalable quantum technologies will require an unprecedented combination of precision and complexity for designing stable structures of well-controllable quantum systems. It is a challenging task to find a suitable elementary building block, of which a quantum network can be comprised in a scalable way. Here we present the working principle of such a basic unit, engineered using molecular chemistry, whose control and readout are executed using a nitrogen vacancy (NV) center in diamond. The basic unit we investigate is a synthetic polyproline with electron spins localized on attached molecular sidegroups separated by a few nanometers. We demonstrate the readout and coherent manipulation of very few ($\leq 6$) of these $S=1/2$ electronic spin systems and access their direct dipolar coupling tensor. Our results show, that it is feasible to use spin-labeled peptides as a resource for a molecular-qubit based network, while at the same time providing simple optical readout of single quantum states through NV-magnetometry. This work lays the foundation for building arbitrary quantum networks using well-established chemistry methods, which has many applications ranging from mapping distances in single molecules to quantum information processing.Comment: Author name typ

Socio-spatial dimensions in energy transitions: Applying the TPSN framework to case studies in Germany

Energy transitions cannot be fully grasped without appreciating their spatial implications. This paper takes up the idea of conceptualizing the socio-spatial dimensions of energy transitions and examines the respective value of the Territory, Place, Scale, and Network (TPSN) framework. The fundamental contribution of this framework is to move the focus of the debate away from whether one ontology of the socio-spatial is ‘better’ than another. By applying the TPSN framework to emblematic cases of regional energy spaces in Germany, we realized that the dynamics of different fields of action within an energy transition are characterized in each case by a specific pattern of the four socio-spatial dimensions and related strategies. The paper concludes with a discussion of the benefits and the shortcomings of the framework as it relates to understanding energy transitions. The fundamental role of place-making at a nexus with territorializations, as well as the additional importance of networking and rescaling strategies, are to be understood with the additional factors of the role of governance spaces, the upscaling of local experiments, powerful space-related discourses, and the socio-materiality of spaces

Mycobacterium bovis detection in slaughtered pigs in the state of Rio Grande do Sul, Brazil

The infection by the Mycobacterium genus is important in pig farming due to the economic losses caused by total or partial carcass condemnation in slaughterhouses. The present study investigated the occurrence of a tuberculosis outbreak in pigs, based on the identification of lesions at the slaughter line of a slaughterhouse. At the inspection line of the slaughterhouse, carcasses were identified with viscera containing macroscopic lesions that indicated tuberculosis (granulomatous lymphadenitis). Tracheobronchial, mesenteric, and submandibular lymph nodes were collected, as well as liver samples and their corresponding lymph nodes. The samples were sent to the Federal Agricultural Defense Laboratory (LFDA/RS) and processed for the diagnosis of tuberculosis and the molecular characterization of Mycobacterium bovis. Based on the results of post-mortem and laboratory inspections, the occurrence was characterized as a tuberculosis outbreak in pigs, which originated from a farm in the state of Rio Grande do Sul, Brazil. Over three months, three batches, adding up to 2884 animals, were sent to slaughter, of which 102 (3.5%) had tuberculosis-like lesions at the inspection line. Based on these results, the productive process was investigated, assessing the feeding, water supply, and milk whey offered in the diet of pigs. It was concluded that the outbreak was caused by feeding unpasteurized or inadequately pasteurized (insufficient time x temperature relation) whey to the pigs. The use of whey from cheese production is a frequent practice in the state of Rio Grande do Sul and one of the risk factors for granulomatous lymphadenitis in pigs

Risk Factors for Alveolar Echinococcosis in Humans

A case-control study of alveolar echinococcosis cases in Germany identifies several risk factors for the disease

Data-Seeking Behaviour in the Social Sciences

Purpose: Publishing research data for reuse has become good practice in recent years. However, not much is known on how researchers actually find said data. In this exploratory study, we observe the information-seeking behaviour of social scientists searching for research data to reveal impediments and identify opportunities for data search infrastructure. Methods: We asked 12 participants to search for research data and observed them in their natural environment. The sessions were recorded. Afterwards, we conducted semi-structured interviews to get a thorough understanding of their way of searching. From the recordings, we extracted the interaction behaviour of the participants and analysed the spoken words both during the search task and the interview by creating affinity diagrams. Results: We found that literature search is more closely intertwined with dataset search than previous literature suggests. Both the search itself and the relevance assessment are very complex, and many different strategies are employed, including the creatively "misuse" of existing tools, since no appropriate tools exist or are unknown to the participants. Conclusion: Many of the issues we found relate directly or indirectly to the application of the FAIR principles, but some, like a greater need for dataset search literacy, go beyond that. Both infrastructure and tools offered for dataset search could be tailored more tightly to the observed work processes, particularly by offering more interconnectivity between datasets, literature, and other relevant materials

Know What Not To Know: Users' Perception of Abstaining Classifiers

Machine learning systems can help humans to make decisions by providing decision suggestions (i.e., a label for a datapoint). However, individual datapoints do not always provide enough clear evidence to make confident suggestions. Although methods exist that enable systems to identify those datapoints and subsequently abstain from suggesting a label, it remains unclear how users would react to such system behavior. This paper presents first findings from a user study on systems that do or do not abstain from labeling ambiguous datapoints. Our results show that label suggestions on ambiguous datapoints bear a high risk of unconsciously influencing the users' decisions, even toward incorrect ones. Furthermore, participants perceived a system that abstains from labeling uncertain datapoints as equally competent and trustworthy as a system that delivers label suggestions for all datapoints. Consequently, if abstaining does not impair a system's credibility, it can be a useful mechanism to increase decision quality