Complexity and dynamics of the winemaking bacterial communities in berries, musts, and wines from apulian grape cultivars through time and space

Currently, there is very little information available regarding the microbiome associated with the wine production chain. Here, we used an amplicon sequencing approach based on high-throughput sequencing (HTS) to obtain a comprehensive assessment of the bacterial community associated with the production of three Apulian red wines, from grape to final product. The relationships among grape variety, the microbial community, and fermentation was investigated. Moreover, the winery microbiota was evaluated compared to the autochthonous species in vineyards that persist until the end of the winemaking process. The analysis highlighted the remarkable dynamics within the microbial communities during fermentation. A common microbial core shared among the examined wine varieties was observed, and the unique taxonomic signature of each wine appellation was revealed. New species belonging to the genus Halomonas were also reported. This study demonstrates the potential of this metagenomic approach, supported by optimized protocols, for identifying the biodiversity of the wine supply chain. The developed experimental pipeline offers new prospects for other research fields in which a comprehensive view of microbial community complexity and dynamics is desirable.Peer ReviewedPostprint (published version

Influence of Splitter Blades on the Flow Field of a Centrifugal Pump: Test-Analysis Comparison

This work aims at studying the influence of adding splitter blades on the performance of a hydraulic centrifugal pump. The studied machine is an ENSIVAL-MORET MP 250.200.400 pump (diameter = 408 mm, 5 blades, specific speed = 32), whose impeller is designed with and without splitter blades. Velocity and pressure fields are computed using unsteady Reynolds-averaged Navier-Stokes (URANS) approach at different flow rates. The sliding mesh method is used to model the rotor zone motion in order to simulate the impeller-volute casing interaction. The flow morphology analysis shows that, when adding splitter blades to the impeller, the impeller periphery velocities and pressures become more homogeneous. An evaluation of the static pressure values all around the impeller is performed and their integration leads to the radial thrust. Global and local experimental validations are carried out at the rotating speed of 900 rpm, for both the original and the splitter blade impellers. The head is evaluated at various flow rates: 50%, 80%, 100%, and 120% of the flow rate at the best efficiency point (BEP). The pressure fluctuations are measured at four locations at the BEP using dynamic pressure sensors. The experimental results match the numerical predictions, so that the effect of adding splitter blades on the pump is acknowledged. Adding splitters has a positive effect on the pressure fluctuations which decrease at the canal duct

Development of an Automated Detection Tool for Healthcare-Associated Infections Based on Screening Natural Language Medical Reports

Poster at AMIA Sym

Health multi-terminology portal: a semantic added-value for patient safety.

International audienceSince the mid-90s, several quality-controlled health gateways were developed. In France, CISMeF is the leading health gateway. It indexes Internet resources from the main institutions, using the MeSH thesaurus and the Dublin Core metadata element set. Since 2005, the CISMeF Information System (IS) includes 24 health terminologies, classifications and thesauri for indexing and information retrieval. This work aims at creating a Health Multi-Terminology Portal (HMTP) and connect it to the CISMeF Terminology Database mainly for searching concepts and terms among all the health controlled vocabularies available in French (or in English and translated in French) and browsing it dynamically. To integrate the terminologies in the CISMeF IS, three steps are necessary: (1) designing a meta-model into which each terminology can be integrated, (2) developing a process to include terminologies into the HMTP, (3) building and integrating existing and new inter-terminology mappings into the HMTP. A total of 24 terminologies are included in the HMTP, with 575,300 concepts, 852,000 synonyms, 222,800 definitions and 1,180,000 relations. Heightteen of these terminologies are not included yet in the UMLS among them, some from the World Health Organization. Since January 2010, HMTP is daily used by CISMeF librarians to index in multi-terminology mode. A health multiterminology portal is a valuable tool helping the indexing and the retrieval of resources from a quality-controlled patient safety gateway. It can also be very useful for teaching or performing audits in terminology management

Stabilization treatment of cultural heritage artefacts : In situ monitoring of marine iron objects dechlorinated in alkali solution

International audienceThe preservation of archaeological iron artefacts necessitates stabilization treatments. For this study, such treatments were applied, in aerated and deaerated NaOH solutions, to Roman iron bars excavated from a marine site in order to understand the evolution of corrosion layers composed of ferrous hydroxychloride β-Fe2(OH)3Cl. Their transformation was monitored in situ by micro-diffraction under synchrotron radiation and characterized ex situ using multi-scale complementary analytical tools. The formation of transient phases such as ferrous hydroxide Fe(OH)2 was identified in both media and chlorinated green rust GR(Cl−) occurred in aerated solutions, showing the influence of dissolved oxygen on the transformation processes

L'apport d'un serveur multi-terminologique de santé pour un codage standardisé de données épidémiologiques - Exemple d'application en épidémiologie hospitalière

National audienc