Predicing Ecological Effects of Watershed-Wide Rain Garden Implementation Using a Low-Cost Methodology

Stormwater control measures (SCMs) have been employed to mitigate peak flows and pollutants ssociated with watershed urbanization. Downstream ecological effects caused by the implementation of SCMs are largely unknown, especially at the watershed scale. Knowledge of these effects could help with setting goals for and targeting locations of local restoration efforts. Unfortunately, studies such as these typically require a high level of time and effort for the investigating party, of which resources are often limited. This study proposes a low-cost investigation method for the prediction of ecological effects on the watershed scale with the implementation of rain garden systems by using publicly available data and software. For demonstration purposes, a typical urban watershed was modeled using Storm Water Management Model (SWMM) 5.0. Forty-five models were developed in which the percent impervious area was varied 3 to 80%, and the fraction of rain gardens implemented with respect to the number of structures was varied from to 100%. The river chub fish (Nocomis micropogon) and its congeners (Nocomis spp.) were chosen as ecological indicators, as they are considered to be keystone species through interspecific nesting association. Depth and velocity criteria for successful nest building locations of the river chub were determined; these criteria can then be applied to many other watersheds. In this study, both base flow conditions and a typical summer storm event (1.3 cm, 6 h duration) were evaluated. During the simulated storm, nest-building locations were not affected in the 3 and 5% impervious cover models. Nest destruction was found to occur in approximately 54% of the original nest building sites for the 9% and 10% impervious areas. Nearly all of the nest-building locations were uninhabitable for impervious areas 20% and greater. Rain garden implementation significantly improved river chub habitat in the simulation, with greatest marginal benefit at lower levels of implementation

Reduced glycogen availability is associated with increased AMPKα2 activity, nuclear AMPKα2 protein abundance, and GLUT4 mRNA expression in contracting human skeletal muscle

Glycogen availability can influence glucose transporter 4 (GLUT4) expression in skeletal muscle through unknown mechanisms. The multisubstrate enzyme AMP-activated protein kinase (AMPK) has also been shown to play an important role in the regulation of GLUT4 expression in skeletal muscle. During contraction, AMPK [alpha]2 translocates to the nucleus and the activity of this AMPK isoform is enhanced when skeletal muscle glycogen is low. In this study, we investigated if decreased pre-exercise muscle glycogen levels and increased AMPK [alpha]2 activity reduced the association of AMPK with glycogen and increased AMPK [alpha]2 translocation to the nucleus and GLUT4 mRNA expression following exercise. Seven males performed 60 min of exercise at ~70% [VO.sub.2] peak on 2 occasions: either with normal (control) or low (LG) carbohydrate pre-exercise muscle glycogen content. Muscle samples were obtained by needle biopsy before and after exercise. Low muscle glycogen was associated with elevated AMPK [alpha]2 activity and acetyl-CoA carboxylase [beta] phosphorylation, increased translocation of AMPK [alpha]2 to the nucleus, and increased GLUT4 mRNA. Transfection of primary human myotubes with a constitutively active AMPK adenovirus also stimulated GLUT4 mRNA, providing direct evidence of a role of AMPK in regulating GLUT4 expression. We suggest that increased activation of AMPK [alpha]2 under conditions of low muscle glycogen enhances AMPK [alpha]2 nuclear translocation and increases GLUT4 mRNA expression in response to exercise in human skeletal muscle. <br /

Increase in Caesarean Deliveries after the Australian Private Health Insurance Incentive Policy Reforms

AbstractBackground: The Australian Private Health Insurance Incentive (PHII) policy reforms implemented in 1997–2000 increased PHI membership in Australia by 50%. Given the higher rate of obstetric interventions in privately insured patients, the reforms may have led to an increase in surgical deliveries and deliveries with longer hospital stays. We aimed to investigate the effect of the PHII policy introduction on birth characteristics in Western Australia (WA).Methods and Findings: All 230,276 birth admissions from January 1995 to March 2004 were identified from administrative birth and hospital data-systems held by the WA Department of Health. Average quarterly birth rates after the PHII introduction were estimated and compared with expected rates had the reforms not occurred. Rate and percentage differences (including 95% confidence intervals) were estimated separately for public and private patients, by mode of delivery, and by length of stay in hospital following birth. The PHII policy introduction was associated with a 20% (221.4 to219.3) decrease in public birth rates, a 51% (45.1 to 56.4) increase in private birth rates, a 5% (25.3 to 25.1) and 8% (28.9 to 27.9) decrease in unassisted and assisted vaginal deliveries respectively, a 5% (25.3 to 25.1) increase in caesarean sections with labour and 10% (8.0 to 11.7) increase in caesarean sections without labour. Similarly, birth rates where the infant stayed 0–3 days in hospital following birth decreased by 20% (221.5 to 218.5), but rates of births with .3 days inhospital increased by 15% (12.2 to 17.1).Conclusions: Following the PHII policy implementation in Australia, births in privately insured patients, caesarean deliveries and births with longer infant hospital stays increased. The reforms may not have been beneficial for quality obstetric care in Australia or the burden of Australian hospitals

Applying ecological systems theory to juvenile legal system interventions outcomes research: a measurement framework

Intervention research and development for youth in the juvenile legal system (JLS) has often focused on recidivism as the primary outcome of interest. Although recidivism is an important outcome, it is ultimately a downstream marker of success and is affected by changes in other domains of youths’ lives (e.g., family and peer relations, neighborhood safety, local and state-level policies). Thus, the present manuscript proposes the application of ecological systems theory to selecting outcomes to assess intervention effects in JLS intervention research to better capture proximal and distal influences on youth behavior. To that end, we first provide an overview of the strengths and limitations of using recidivism as an outcome measure. Next, the current application of social ecology theory to existing research on both risk and protective factors of JLS involvement is discussed, as well as existing work on assessing social-ecological domains within intervention studies. Then, a measurement framework is introduced for selecting pertinent domains of youths’ social ecologies to assess as intervention outcomes, moderators, and mediators. To facilitate this, we provide examples of concrete constructs and measures that researchers may select. We conclude with potential new avenues of research to which our proposed framework could lead, as well as potential limitations of implementing our framework

Enantioselective Inhibition of Human Papillomavirus L1 Pentamer Formation by Chiral-Proline Modified Calix[4]arenes: Targeting the Protein Interface

Although current human papillomavirus (HPV) vaccines can protect well against infection, they are effective only for a limited number of subtypes. Coupled with the dilemma that no efficient prescription is currently clinically available for therapy, there is an urgent need to develop new anti-HPV agents. In the present study l- and d-Proline modified calix[4]arenes (Pro- C4 A) were investigated to determine any differences in their effect on the assembly of HPV 16 L1 pentamer (L1-p). The mechanism of action using model peptides was investigated by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Nuclear Magnetic Resonance (NMR) and revealed that the binding was targeting the basic residues at L1 interface. This was also well supported by the trypsin digestion experiments and molecular simulations performed on the full-le ngth L1. The large energy and morphology differences revealed by molecular simulations explain the binding disparity of l- and d-Pro-C4 A to L1, and consequently the selective inhibition of them on L1-p forma- tion. The present study opens a way to develop enantioselec- tive and cost-effective inhibitors for L1-p formation, which might be used as a new kind of anti-HPV agent and could be extended to other viruses based on similar mechanisms

Leukotriene antagonists as first-line or add-on asthma controller therapy

Most randomized trials of treatment for asthma study highly selected patients under idealized conditions. METHODS: We conducted two parallel, multicenter, pragmatic trials to evaluate the real-world effectiveness of a leukotriene-receptor antagonist (LTRA) as compared with either an inhaled glucocorticoid for first-line asthma-controller therapy or a long-acting beta(2)-agonist (LABA) as add-on therapy in patients already receiving inhaled glucocorticoid therapy. Eligible primary care patients 12 to 80 years of age had impaired asthma-related quality of life (Mini Asthma Quality of Life Questionnaire [MiniAQLQ] score =6) or inadequate asthma control (Asthma Control Questionnaire [ACQ] score =1). We randomly assigned patients to 2 years of open-label therapy, under the care of their usual physician, with LTRA (148 patients) or an inhaled glucocorticoid (158 patients) in the first-line controller therapy trial and LTRA (170 patients) or LABA (182 patients) added to an inhaled glucocorticoid in the add-on therapy trial. RESULTS: Mean MiniAQLQ scores increased by 0.8 to 1.0 point over a period of 2 years in both trials. At 2 months, differences in the MiniAQLQ scores between the two treatment groups met our definition of equivalence (95% confidence interval [CI] for an adjusted mean difference, -0.3 to 0.3). At 2 years, mean MiniAQLQ scores approached equivalence, with an adjusted mean difference between treatment groups of -0.11 (95% CI, -0.35 to 0.13) in the first-line controller therapy trial and of -0.11 (95% CI, -0.32 to 0.11) in the add-on therapy trial. Exacerbation rates and ACQ scores did not differ significantly between the two groups. CONCLUSIONS: Study results at 2 months suggest that LTRA was equivalent to an inhaled glucocorticoid as first-line controller therapy and to LABA as add-on therapy for diverse primary care patients. Equivalence was not proved at 2 years. The interpretation of results of pragmatic research may be limited by the crossover between treatment groups and lack of a placebo group

Ultracompact HII regions with extended emission: The case of G43.89-0.78 and its molecular environment

The Karl Jansky Very Large Array (VLA), Owens Valley Radio Observatory (OVRO), Atacama Large Millimetric Array (ALMA), and the infrared \textit{Spitzer} observatories, are powerful facilities to study massive star formation regions and related objects such as ultra--compact (UC) \hii regions, molecular clumps, and cores. We used these telescopes to study the \uchiir G43.89--0.78. The morphological study at arcminute scales using NVSS and \textit{Spitzer} data shows that this region is similar to those observed in the \textit{ bubble--like} structures revealed by \textit{Spitzer} observations. With this result, and including a physical characterization based on 3.6 cm data, we suggest G43.89--0.78 be classified as an \uchiir with Extended Emission because it meets the operational definition given in this paper comparing radio continuum data at 3.6 and 20~cm. For the ultra-compact component, we use VLA data to obtain physical parameters at 3.6~cm confirming this region as an \uchii region. Using ALMA observations, we detect the presence of a dense ($2.6\times10^7$ cm$^{-3}$) and small ($\sim$ 2.0\arcsec; 0.08 pc) molecular clump with a mass of 220 M$_{\odot}$ and average kinetic temperature of 21~K, located near to the \uchii region. In this clump, catalogued as G43.890--0.784, water masers also exist, possibly tracing a bipolar outflow. We discover in this vicinity two additional clumps which we label as G43.899--0.786 (T$_d$ = 50 K; M = 11 M$_{\odot}$), and G43.888--0.787 (T$_d$ = 50 K; M = 15 M$_{\odot}$).Comment: 13 pages, 8 figures, 2 tables. Accepted for publication in the Monthly Notices of the Royal Astronomical Society Main Journal (2020

Dynamics of Sleep-Wake Transitions During Sleep

We study the dynamics of the awakening during the night for healthy subjects and find that the wake and the sleep periods exhibit completely different behavior: the durations of wake periods are characterized by a scale-free power-law distribution, while the durations of sleep periods have an exponential distribution with a characteristic time scale. We find that the characteristic time scale of sleep periods changes throughout the night. In contrast, there is no measurable variation in the power-law behavior for the durations of wake periods. We develop a stochastic model which agrees with the data and suggests that the difference in the dynamics of sleep and wake states arises from the constraints on the number of microstates in the sleep-wake system.Comment: Final form with some small corrections. To be published in Europhysics Letters, vol. 57, issue no. 5, 1 March 2002, pp. 625-63