Estimated performance of the TRIUMF ultracold neutron source and electric dipole moment apparatus

Searches for the permanent electric dipole moment of the neutron (nEDM) provide strong constraints on theories beyond the Standard Model of particle physics. The TUCAN collaboration is constructing a source for ultracold neutrons (UCN) and an apparatus to search for the nEDM at TRIUMF, Vancouver, Canada. In this work, we estimate that the spallation-driven UCN source based on a superfluid helium converter will provide $(1.38\pm0.02) \times 10^7$ polarized UCN at a density of $217\pm3$~UCN/cm$^3$ to a room-temperature EDM experiment per fill. With $(1.51\pm0.02) \times 10^6$ neutrons detected after the Ramsey cycle, the statistical sensitivity for an nEDM search per storage cycle will be $(1.94\pm0.06) \times 10^{-25}\,e$cm (1$\sigma$). The goal sensitivity of $10^{-27}\,e$cm (1$\sigma$) can be reached within $280\pm16$ measurement days.Comment: Proceedings submitted to EPJ Web of Conferences for the Symposium on Symmetries in Subatomic Physics (SSP2022

Irradiated fibroblasts increase interleukin-6 expression and induce migration of head and neck squamous cell carcinoma

Background Cytotoxic effects of radiation play an important role in the treatment of head and neck cancer. However, irradiation is known to lead to the migration of various cancer cells, including those of head and neck cancer. Recently, fibroblasts in the cancer microenvironment have been reported to be involved in this mechanism. Nevertheless, the mechanism underlying migration of head and neck cancer cells remains unclear. Herein, we aimed to elucidate this migration mechanism induced by irradiation in terms of the interaction of head and neck cancer cells with fibroblasts. Methods We used the head and neck squamous cell carcinoma (HNSCC) cell lines SAS and FaDu as well as fibroblast cell lines. These cells were irradiated and their viability was compared. In fibroblasts, changes in interleukin-6 (IL-6) secretion caused by irradiation were measured by enzyme-linked immunosorbent assay (ELISA). The cell migration ability of cancer cells was evaluated via a migration assay using a semipermeable membrane. HNSCC cells were cocultured with irradiated and nonirradiated fibroblasts, and their migration ability under each condition was compared. We also examined the effect of IL-6 on the migration of HNSCC cells. Furthermore, to investigate the effect of fibroblast-derived IL-6 on the migration ability of HNSCC cells, we conducted a coculture study using IL-6 neutralizing antibody. Results Irradiation reduced the survival of HNSCC cells, whereas fibroblasts were resistant to irradiation. Irradiation also increased IL-6 secretion by fibroblasts. Migration of HNSCC cells was enhanced by coculture with fibroblasts and further enhanced by coculture with irradiated fibroblasts. We also confirmed that the migration of HNSCC cells was induced by IL-6. The enhanced migration of cancer cells caused by coculturing with fibroblasts was canceled by the IL-6 neutralizing antibody. Conclusion These results show that fibroblasts survive irradiation and induce the migration ability of HNSCC cells through increased secretion of IL-6

1-Year Results of the ZEPHYR Registry (Zilver PTX for the Femoral Artery and Proximal Popliteal Artery) Predictors of Restenosis

AbstractObjectivesThis study sought to assess the rate and predictors of 1-year restenosis after drug-eluting stent implantation for femoropopliteal (FP) lesions in patients with peripheral arterial disease.BackgroundZilver PTX, a paclitaxel-eluting stent for FP lesions, provides superior outcomes to angioplasty and bare-metal stents in clinical trials. However, its real-world outcomes and the associated features remain unclear.MethodsThis was a prospective multicenter study enrolling 831 FP lesions (797 limbs, 690 patients) treated by Zilver PTX implantation. The primary endpoint was 1-year restenosis. Secondary endpoints included major adverse limb event and stent thrombosis.ResultsMean lesion length was 17 ± 10 cm. One-year restenosis, major adverse limb event, and stent thrombosis rates were 37%, 22%, and 2%, respectively. The generalized linear mixed model showed that lesion length ≥16 cm assessed by angiography and distal external elastic membrane area ≤27 mm2 and minimum stent area ≤12 mm2 assessed by intravascular ultrasound were independent risk factors for restenosis. One-year restenosis rates were 15% in cases with none of these risk factors and 50% in those with ≥2 risk factors.ConclusionsThe current study demonstrated 1-year real-world outcomes after drug-eluting stent treatment for FP lesions, including challenging ones in clinical practice. Lesion length, external elastic membrane area, and minimum stent area were independent predictors for restenosis. (Zilver PTX for the Femoral Artery and Proximal Popliteal Artery—Prospective Multicenter Registry [ZEPHYR]; UMIN000008433

HAMAMATSU-ICG study: Protocol for a phase III, multicentre, single-arm study to assess the usefulness of indocyanine green fluorescent lymphography in assessing secondary lymphoedema

Introduction Secondary lymphoedema of the extremities is an important quality-of-life issue for patients who were treated for their malignancies. Indocyanine green (ICG) fluorescent lymphography may be helpful for assessing lymphoedema and for planning lymphaticovenular anastomosis (LVA). The objective of the present clinical trial is to confirm whether or not ICG fluorescent lymphography using the near-infrared monitoring camera is useful for assessing the indication for LVA, for the identification of the lymphatic vessels before the conduct of LVA, and for the confirmation of the patency of the anastomosis site during surgery. Methods and analysis This trial is a phase III, multicentre, single-arm, open-label clinical trial to assess the efficacy and safety of ICG fluorescent lymphography when assessing and treating lymphoedema of patients with secondary lymphoedema who are under consideration for LVA. The primary endpoint is the identification rate of the lymphatic vessels at the incision site based on ICG fluorescent lymphograms obtained before surgery. The secondary endpoints are 1) the sensitivity and specificity of dermal back flow determined by ICG fluorescent lymphography as compared with 99mTc lymphoscintigraphy—one of the standard diagnostic methods and 2) the usefulness of ICG fluorescent lymphography when confirming the patency of the anastomosis site after LVA. Ethics and dissemination The protocol for the study was approved by the Institutional Review Board of each institution. The trial was filed for and registered at the Pharmaceuticals and Medical Devices Agency in Japan. The trial is currently on-going and is scheduled to end in June 2020

Study of Thin Iron Films for Polarization Analysis of Ultracold Neutrons

The TUCAN (TRIUMF Ultra-Cold Advanced Neutron) collaboration aims to search for the neutron electric dipole moment (nEDM) with unprecedented precision. One of the essential elements for the nEDM measurement is a polarization analyzer of ultracold neutrons (UCNs), whose main component is a magnetized thin iron film. Several thin iron films were deposited on aluminum and silicon ubstrates and were characterized by vibrating sample magnetometry and cold-neutron reflectometry. A magnetic field required to saturate the iron film is $\sim$12 kA/m for those on the aluminum substrates and 6.4 kA/m for the silicon substrates. The magnetic potential of the iron films on the Si substrate was estimated to be 2 T by the neutron reflectometry, which is sufficient performance for an UCN polarization analyzer of the nEDM measurement.Comment: Proceedings of the 24th International Spin Symposium (SPIN 2021), 18-22 October 2021, Matsue, Japa

Clinical Outcomes of Cetuximab and Paclitaxel after Progression on Immune Checkpoint Inhibitors in Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma

Background and Objectives: In recent years, the effectiveness of chemotherapy after immune checkpoint inhibitor administration has attracted attention in various cancers, including head and neck cancers. However, individual assessments of the administered chemotherapy regimens are insufficient. This study aimed to evaluate the efficacy and safety of chemotherapy after immune checkpoint inhibitor administration in recurrent metastatic head and neck cancer by focusing on a single regimen. Materials and Methods: We retrospectively reviewed clinical and radiological data from the medical records of 18 patients with recurrent or metastatic (R/M) head and neck squamous cell carcinoma (HNSCC) who received systemic chemotherapy with weekly cetuximab and paclitaxel (Cmab + PTX) after progression following immune checkpoint inhibitor (ICI) therapy. The objective response rate (ORR) and disease control rate (DCR) were assessed using Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1. Progression-free survival (PFS) and overall survival (OS) were estimated using the Kaplan-Meier method. Adverse events (AEs) were recorded using National Cancer Institute Common Terminology Criteria for Adverse Events, version 4.0. Results: In all patients, the ORR, DCR, median PFS, and median OS were 44.4%, 72.2%, 3.8 months, and 9.6 months, respectively. Regarding AEs, three patients developed grade 3 neutropenia. Grade 3 anemia, paronychia, asthenia, and peripheral neuropathy were observed in one patient each. There were no treatment-related deaths. Conclusions: Cmab + PTX was shown to maintain high efficacy and acceptable safety for R/M HNSCC that progressed after ICI therapy. Further research is needed to establish optimal treatment sequences and drug combinations for recurrent R/M HNSCC

Preoperative multidisciplinary treatment with hyperthermia for soft tissue sarcoma

We report the results of phase I/II studies of preoperative multidisciplinary treatment of 14 patients with soft tissue sarcoma using hyperthermia from November 1990 to April 1995. The preoperative treatment was conducted with thermo-radio-chemotherapy in 11 cases of stage III, and with thermo-radiotherapy as well as thermo-chemotherapy in three cases of stages I and II. Hyperthermia was carried out twice a week with totals ranging from 4 to 14 times (average: 8.4 times); each session lasted 60min. Radiotherapy was administered four or five times per week, and the dose was 1.8 2Gy/fraction, with a total of 30-40Gy in a four week period. Chemotherapy was mainly in the form of MAID regimen (2-mercaptoethanesulphonic acid (mesna), adriamycin, ifosfamide and dacarbazine). The tumors were surgically resected in all patients after completing the preoperative treatment. The efficacy rate, as expressed by the percentage of either tumors in which reduction rate was 50% or more, or tumors for which post-treatment contrast enhanced CT image revealed low density volumes occupying 50% or more of the total mass, was 71 % (ten of the 14 tumors). The mean tumor necrosis rate in the resected specimens was 78%. The tumor necrosis rate was significantly high (P &#60; 0.05) in patients whose Time &#8805; 42°C was of long duration. Postoperative complications were observed in six patients; among these, two patients developed wound infection that required surgical treatment as a complication of surgery performed in the early stage following the preoperative treatment. After a mean postoperative follow-up of 27 months, distant metastasis occurred in four patients resulting in three fatalities. The three-year cumulative survival rate was 64.3%. No local recurrence was observed in any patient during the follow-up, thus confirming our hypothesis that preoperative multidisciplinary treatment has an excellent local efficacy. We think that it would be valuable to conduct, at many facilities, phase III studies on the treatment of soft tissue sarcoma by a combination of surgery and preoperative multidisciplinary treatment using hyperthermia, paying close attention to the interval between these two modalities.</p

Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice

Physiological properties involved in divergent cadmium (Cd) accumulation among rice genotypes were characterized using the indica cultivar ‘Habataki’ (high Cd in grains) and the japonica cultivar ‘Sasanishiki’ (low Cd in grains). Time-dependence and concentration-dependence of symplastic Cd absorption in roots were revealed not to be responsible for the different Cd accumulation between the two cultivars because root Cd uptake was not greater in the Cd-accumulating cultivar ‘Habataki’ compared with ‘Sasanishiki’. On the other hand, rapid and greater root-to-shoot Cd translocation was observed in ‘Habataki’, which could be mediated by higher abilities in xylem loading of Cd and transpiration rate as a driving force. To verify whether different abilities in xylem-mediated shoot-to-root translocation generally account for the genotypic variation in shoot Cd accumulation in rice, the world rice core collection, consisting of 69 accessions which covers the genetic diversity of almost 32 000 accessions of cultivated rice, was used. The results showed strong correlation between Cd levels in xylem sap and shoots and grains among the 69 rice accessions. Overall, the results presented in this study revealed that the root-to-shoot Cd translocation via the xylem is the major and common physiological process determining the Cd accumulation level in shoots and grains of rice plants

カンタン ニ テキセツ ナ ツヨサ デ ソウチャク デキル ジョウミャク センシ ヨウ クケツタイ ノ カイハツ

看護師が前腕に静脈穿刺をする際にゴム管駆血帯を用いた場合の駆血圧は70-95mmHgが適切と報告されているが、看護師が駆血帯を装着する強さは幅広く分散しており、高すぎる傾向にある。この原因の一つとして、駆血帯には適切な目安が無く、意図した強さで装着することが難しいことが考えられた。そこで、適切なく血圧の指標となるメモリを付けた駆血帯を開発し、その性能について検討した。60名の対象者(平均年齢40.5歳、範囲21-78歳)に駆血帯を上腕周囲径より1-4メモリ短く装着した時の駆血圧の平均はそれぞれ45,76,110,144 mmHgであり、静脈の断面積は3メモリまでは増加したが、4メモリまで短くするとやや減少した。静脈穿刺可能な怒張が得られた対象者はそれぞれ30%,80%,90%,85%で、3メモリまでは増加したが、4メモリ短くするとやや減少した。この駆血帯では上腕周囲径より2-3メモリ短く装着するのが適切と考えられた。When nurses attempt venipuncture at the forearm, they apply a tourniquet at the upper arm to distend the forearm vein. Appropriate gum-tube tourniquet pressure is reported to be between 70-95mmHg. But tourniquet pressure applied by japanese nurses in clinical situations is often too high and widely distributed. One of the reason they do not apply tourniquets with appropriate pressure is that the tourniquets they use have no marks for applying the appropriate pressure. So, we developed a new tourniquet with marks for applying appropriate pressure, and we studied the effectiveness of this tourniquet. Sixty subjects (mean age40.5 years old, range:21-78 years old) were enrolled. When we applied tourniquets with tourniquet lengths 1, 2, 3, and 4 marks shorter than the circumference of the upper arm, mean tourniquet pressures were 45.1±6.7mmHg, 76.0±12.2mmHg,109.6±13.0mmHg, and 143.7±24.7mmHg respectively, and vein cross section area increased according to the stretch strength until 3 marks, then decreased slightly at 4 marks. The percentages of subjects with sufficient vein distention for venipuncture were about 30%, 80%, 90%, and 85% respectively. The results indicated that applying tourniquet with 2 marks or 3 marks shorter is appropriate for venipuncture, while 4 marks is too strong