Ocorrência de Cabassous tatouay (Cingulata, Dasypodidae) e seu potencial de distribuição para o sul do Brasil

Cabassous tatouay Desmarest, 1804 é considerada espécie rara no sul da América do Sul, apresentando registros escassos e imprecisos para o Rio Grande do Sul. O presente estudo descreve 40 localidades de ocorrência de C. tatouay e apresenta de um mapa de distribuição geográfica potencial, gerado por Modelagem Ecológica de Nicho. A modelagem de nicho sugere uma associação da espécie com áreas de matriz campestre, incluindo o Pampa e os Campos de Cima da Serra, associados à Mata Atlântica. Este estudo contribui para o melhor conhecimento do tatu-de-rabo-mole no Sul do Brasil e fornece dados-chave para sua conservação.Cabossous tatouay Desmarest, 1804 is considered a rare species in southern South America, and Rio Grande do Sul State, Brazil, records of the species are scarce and inaccurate. This study reports 40 localities for C. tatouay, and provides a map of the species' potential distribution using ecological niche modeling (ENM). The ENM indicated that in this region C. tatouay is associated with open grasslands, including the areas of "Pampas" and the open fields in the highlands of the Atlantic Forest. This study contributes to the information about the greater naked-tailed armadillo in southern Brazil, and provides data key to its future conservation

Multidimensional cosmological models: cosmological and astrophysical implications and constraints

We investigate four-dimensional effective theories which are obtained by dimensional reduction of multidimensional cosmological models with factorizable geometry and consider the interaction between conformal excitations of the internal space (geometrical moduli excitations) and Abelian gauge fields. It is assumed that the internal space background can be stabilized by minima of an effective potential. The conformal excitations over such a background have the form of massive scalar fields (gravitational excitons) propagating in the external spacetime. We discuss cosmological and astrophysical implications of the interaction between gravexcitons and four-dimensional photons as well as constraints arising on multidimensional models of the type considered in our paper. In particular, we show that due to the experimental bounds on the variation of the fine structure constant, gravexcitons should decay before nucleosynthesis starts. For a successful nucleosynthesis the masses of the decaying gravexcitons should be m>10^4 GeV. Furthermore, we discuss the possible contribution of gravexcitons to UHECR. It is shown that, at energies of about 10^{20}eV, the decay length of gravexcitons with masses m>10^4 GeV is very small, but that for m <10^2 GeV it becomes much larger than the Greisen-Zatsepin-Kuzmin cut-off distance. Finally, we investigate the possibility for gravexciton-photon oscillations in strong magnetic fields of astrophysical objects. The corresponding estimates indicate that even the high magnetic field strengths of magnetars are not sufficient for an efficient and copious production of gravexcitons.Comment: 16 pages, LaTeX2e, minor changes, improved references, to appear in PR

Eighteen Years of Molecular Genotyping the Hemophilia Inversion Hotspot: From Southern Blot to Inverse Shifting-PCR

The factor VIII gene (F8) intron 22 inversion (Inv22) is a paradigmatic duplicon-mediated rearrangement, found in about one half of patients with severe hemophilia A worldwide. The identification of this prevalent cause of hemophilia was delayed for nine years after the F8 characterization in 1984. The aim of this review is to present the wide diversity of practical approaches that have been developed for genotyping the Inv22 (and related int22h rearrangements) since discovery in 1993. The sequence— Southern blot, long distance-PCR and inverse shifting-PCR—for Inv22 genotyping is an interesting example of scientific ingenuity and evolution in order to resolve challenging molecular diagnostic problems

Measurement of the cosmic ray spectrum above 4×10184{\times}10^{18} eV using inclined events detected with the Pierre Auger Observatory

A measurement of the cosmic-ray spectrum for energies exceeding $4{\times}10^{18}$ eV is presented, which is based on the analysis of showers with zenith angles greater than $60^{\circ}$ detected with the Pierre Auger Observatory between 1 January 2004 and 31 December 2013. The measured spectrum confirms a flux suppression at the highest energies. Above $5.3{\times}10^{18}$ eV, the "ankle", the flux can be described by a power law $E^{-\gamma}$ with index $\gamma=2.70 \pm 0.02 \,\text{(stat)} \pm 0.1\,\text{(sys)}$ followed by a smooth suppression region. For the energy ($E_\text{s}$) at which the spectral flux has fallen to one-half of its extrapolated value in the absence of suppression, we find $E_\text{s}=(5.12\pm0.25\,\text{(stat)}^{+1.0}_{-1.2}\,\text{(sys)}){\times}10^{19}$ eV.Comment: Replaced with published version. Added journal reference and DO

A GRFa2/Prop1/Stem (GPS) Cell Niche in the Pituitary

BACKGROUND: The adult endocrine pituitary is known to host several hormone-producing cells regulating major physiological processes during life. Some candidates to progenitor/stem cells have been proposed. However, not much is known about pituitary cell renewal throughout life and its homeostatic regulation during specific physiological changes, such as puberty or pregnancy, or in pathological conditions such as tumor development. PRINCIPAL FINDINGS: We have identified in rodents and humans a niche of non-endocrine cells characterized by the expression of GFRa2, a Ret co-receptor for Neurturin. These cells also express b-Catenin and E-cadherin in an oriented manner suggesting a planar polarity organization for the niche. In addition, cells in the niche uniquely express the pituitary-specific transcription factor Prop1, as well as known progenitor/stem markers such as Sox2, Sox9 and Oct4. Half of these GPS (GFRa2/Prop1/Stem) cells express S-100 whereas surrounding elongated cells in contact with GPS cells express Vimentin. GFRa2+-cells form non-endocrine spheroids in culture. These spheroids can be differentiated to hormone-producing cells or neurons outlining the neuroectoderm potential of these progenitors. In vivo, GPSs cells display slow proliferation after birth, retain BrdU label and show long telomeres in its nuclei, indicating progenitor/stem cell properties in vivo. SIGNIFICANCE: Our results suggest the presence in the adult pituitary of a specific niche of cells characterized by the expression of GFRa2, the pituitary-specific protein Prop1 and stem cell markers. These GPS cells are able to produce different hormone-producing and neuron-like cells and they may therefore contribute to postnatal pituitary homeostasis. Indeed, the relative abundance of GPS numbers is altered in Cdk4-deficient mice, a model of hypopituitarism induced by the lack of this cyclin-dependent kinase. Thus, GPS cells may display functional relevance in the physiological expansion of the pituitary gland throughout life as well as protection from pituitary disease

Genetic effects on gene expression across human tissues

Characterization of the molecular function of the human genome and its variation across individuals is essential for identifying the cellular mechanisms that underlie human genetic traits and diseases. The Genotype-Tissue Expression (GTEx) project aims to characterize variation in gene expression levels across individuals and diverse tissues of the human body, many of which are not easily accessible. Here we describe genetic effects on gene expression levels across 44 human tissues. We find that local genetic variation affects gene expression levels for the majority of genes, and we further identify inter-chromosomal genetic effects for 93 genes and 112 loci. On the basis of the identified genetic effects, we characterize patterns of tissue specificity, compare local and distal effects, and evaluate the functional properties of the genetic effects. We also demonstrate that multi-tissue, multi-individual data can be used to identify genes and pathways affected by human disease-associated variation, enabling a mechanistic interpretation of gene regulation and the genetic basis of diseas