Etude écotoxicologique et phylogéographique de la coque Cerastoderma glaucum issue du Golfe de Gabès : réponse adaptative (in situ et in vivo) au stress métallique et structure génétique

Cerastoderma glaucum is an adequate biological model for phylogeographic and ecotoxicological studies.A study of its genetic structure in the Western Mediterranean sea was conducted using two markers types: mitochondrial and nuclear. The DNA mitochondrial marker results show a divergent population from all the other ones (Ellouza South ofTunisia).An ecotoxicological study of populations sampled from Ellouza site and two other nearby sites in the Gulf of Gabes region was carried out in order to study the adaptive response of natural populations of C. glaucum at different levels of biological organization from the individual level to the cellular and molecular ones. In parallel, two laboratory experiments were performed to clarify the mechanisms that may explain the success or failure of maintaining populations of C. glaucum. In thefirst one, industrial effluents were used and in the second we have used a pure contaminant, the cadmium. These studies experiments concerned individual and molecular levels. Different biomarkers involved in the response to different types of stress: (MT), (ABCB1) (MnSOD and CuZnSOD) (CAT) (HSP70) and (COI) were used in natural and exposed cockles.Results showed the importance of energy metabolism in response to stress whatever the used approach (in situ / Experimental) through the regulation of the expression of the COI gene.Cerastoderma glaucum est un modèle biologique de choix pour des études phylogéographiques et écotoxicologiques.Une étude de sa structure génétique en méditerranée occidentale a été réalisée en utilisant deux types de marqueurs, mitochondrial et nucléaire. Les résultats obtenus ont révélé à travers l’ADN mitochondrial une population très divergente de toutes les autres populations (Ellouza, Sud de la Tunisie).Une étude écotoxicologique sur des populations issues de ce site Ellouza et deux autres sites à proximité dans la région du golfe de Gabès a été réalisée dans le but d’étudier la réponse adaptative des populations de la coque C. glaucum en milieu naturel à différents niveaux de l’organisation biologique allant du niveau individuel jusqu’au niveau cellulaire et moléculaire. En parallèle à cette étude, deux expérimentations en laboratoire ont été réalisées afin de préciser les mécanismes pouvant expliquer le succès ou l’échec du maintien des populations de C. glaucum. La première expérimentation a été réalisée en utilisant des effluents industriels et la deuxième en utilisant un contaminant pur le cadmium. Les niveaux de l’organisationbiologique concernés par cette étude étaient le niveau individuel et le niveau moléculaire. Différents biomarqueurs impliqués dans la réponse à différents types de stress : (MT), (ABCB1), (MnSOD et CuZnSOD), (CAT), (HSP70) et (COI) ont été utilisés en milieu naturel ainsi qu’en conditions contrôlées et les résultats obtenus ont montré l’importance dumétabolisme énergétique dans la réponse au stress quelque soit l’approche utilisée (in situ/ expérimental) à travers la régulation de l’expression du gène COI

First record of the Gracile lizardfish Saurida gracilis (Quoy & Gaimard, 1824) in Mediterranean waters

In January 2021 during a scientific survey to assess the economic impacts of a Mass Mortality Event, a single individual of the Gracile lizardfish Saurida gracilis (Quoy & Gaimard, 1824) was caught out of Korbous (Northern Tunisia). This observation, confirmed by genetic analysis, provides evidence on the occurrence of a new non-indigenous species in Mediterranean waters. The relevance of this finding for the current monitoring strategies implemented at the regional level are briefly raised and discussed

Unpublished Mediterranean and Black Sea records of marine alien, cryptogenic, and neonative species

To enrich spatio-temporal information on the distribution of alien, cryptogenic, and neonative species in the Mediterranean and the Black Sea, a collective effort by 173 marine scientists was made to provide unpublished records and make them open access to the scientific community. Through this effort, we collected and harmonized a dataset of 12,649 records. It includes 247 taxa, of which 217 are Animalia, 25 Plantae and 5 Chromista, from 23 countries surrounding the Mediterranean and the Black Sea. Chordata was the most abundant taxonomic group, followed by Arthropoda, Mollusca, and Annelida. In terms of species records, Siganus luridus, Siganus rivulatus, Saurida lessepsianus, Pterois miles, Upeneus moluccensis, Charybdis (Archias) longicollis, and Caulerpa cylindracea were the most numerous. The temporal distribution of the records ranges from 1973 to 2022, with 44% of the records in 2020–2021. Lethrinus borbonicus is reported for the first time in the Mediterranean Sea, while Pomatoschistus quagga, Caulerpa cylindracea, Grateloupia turuturu, and Misophria pallida are first records for the Black Sea; Kapraunia schneideri is recorded for the second time in the Mediterranean and for the first time in Israel; Prionospio depauperata and Pseudonereis anomala are reported for the first time from the Sea of Marmara. Many first country records are also included, namely: Amathia verticillata (Montenegro), Ampithoe valida (Italy), Antithamnion amphigeneum (Greece), Clavelina oblonga (Tunisia and Slovenia), Dendostrea cf. folium (Syria), Epinephelus fasciatus (Tunisia), Ganonema farinosum (Montenegro), Macrorhynchia philippina (Tunisia), Marenzelleria neglecta (Romania), Paratapes textilis (Tunisia), and Botrylloides diegensis (Tunisia).peer-reviewe

Ecotoxicology and phylogeography studies of the cockle Cerastoderma glaucum from the gulf of Gabès : adaptive response (in situ and in vivo) to metal stress and genetic structure

Cerastoderma glaucum est un modèle biologique de choix pour des études phylogéographiques et écotoxicologiques.Une étude de sa structure génétique en méditerranée occidentale a été réalisée en utilisant deux types de marqueurs, mitochondrial et nucléaire. Les résultats obtenus ont révélé à travers l’ADN mitochondrial une population très divergente de toutes les autres populations (Ellouza, Sud de la Tunisie).Une étude écotoxicologique sur des populations issues de ce site Ellouza et deux autres sites à proximité dans la région du golfe de Gabès a été réalisée dans le but d’étudier la réponse adaptative des populations de la coque C. glaucum en milieu naturel à différents niveaux de l’organisation biologique allant du niveau individuel jusqu’au niveau cellulaire et moléculaire. En parallèle à cette étude, deux expérimentations en laboratoire ont été réalisées afin de préciser les mécanismes pouvant expliquer le succès ou l’échec du maintien des populations de C. glaucum. La première expérimentation a été réalisée en utilisant des effluents industriels et la deuxième en utilisant un contaminant pur le cadmium. Les niveaux de l’organisationbiologique concernés par cette étude étaient le niveau individuel et le niveau moléculaire. Différents biomarqueurs impliqués dans la réponse à différents types de stress : (MT), (ABCB1), (MnSOD et CuZnSOD), (CAT), (HSP70) et (COI) ont été utilisés en milieu naturel ainsi qu’en conditions contrôlées et les résultats obtenus ont montré l’importance dumétabolisme énergétique dans la réponse au stress quelque soit l’approche utilisée (in situ/ expérimental) à travers la régulation de l’expression du gène COI.Cerastoderma glaucum is an adequate biological model for phylogeographic and ecotoxicological studies.A study of its genetic structure in the Western Mediterranean sea was conducted using two markers types: mitochondrial and nuclear. The DNA mitochondrial marker results show a divergent population from all the other ones (Ellouza South ofTunisia).An ecotoxicological study of populations sampled from Ellouza site and two other nearby sites in the Gulf of Gabes region was carried out in order to study the adaptive response of natural populations of C. glaucum at different levels of biological organization from the individual level to the cellular and molecular ones. In parallel, two laboratory experiments were performed to clarify the mechanisms that may explain the success or failure of maintaining populations of C. glaucum. In thefirst one, industrial effluents were used and in the second we have used a pure contaminant, the cadmium. These studies experiments concerned individual and molecular levels. Different biomarkers involved in the response to different types of stress: (MT), (ABCB1) (MnSOD and CuZnSOD) (CAT) (HSP70) and (COI) were used in natural and exposed cockles.Results showed the importance of energy metabolism in response to stress whatever the used approach (in situ / Experimental) through the regulation of the expression of the COI gene

Des échelles de valorisation spatio-culturelle, d'hydropaysage et d'arquitecture des villages mauresques au Nord de la Tunisie : De l'intégration aux projects territoriaux

Debido a sus especificidades naturales, organizativas y sociales, las ciudades de Testour y Ghar El Melh parecen adquirir una gran influencia, incluso una notoriedad, en otros sitios moriscos implantados en el norte de Túnez. Estas ciudades presentan una identidad cultural muy marcada por intercambios socio-espaciales asociados a un esquema típico de una urbanidad rural precoz. Sin embargo, este tipo de territorio está sujeto a cambios intensos y rápidos, y a una aceleración de los cambios de uso de los medios que imponen una fragilización del patrimonio urbano y agrario por la difícil problemática de conciliación entre la indispensable evolución económica, social y técnica y la salvaguardia del medio ambiente. Frente a las preocupaciones suscitadas por estas fragilizaciones, deben aplicarse nuevas herramientas interdisciplinarias para permitir reconstruir las etapas de construcción socio-socialespacial, por lo tanto, comprender algunos aspectos de la organización de la fabricación y el funcionamiento de los medios. En este marco, proponemos una lógica progresiva que conduce a un proyecto de gestión global, exponiendo un conjunto de procedimientos metodológicos analíticos a través de los cuales se va a trazar una dinámica de estos paisajes auténticos. Para ello, la exposición de un método de investigación geográfica desarrollado al inicio de nuestro estudio consistente en determinar los descriptores de construcción histórico-natural de los sitios de estudio basándose en un enfoque integrado de cartografía e iconografía; resulta indispensable. Con el fin de discernir las ventajas de los paisajes identitarios, iniciamos a continuación un método de diagnóstico paisajístico basado en el concepto de la genialidad del lugar. A continuación, procederemos a un enfoque sintético mediante un seguimiento de los problemas de los sitios y de los indicadores de mutación y reconversión paisajística mediante una encuesta socioeconómica. También se ha integrado un enfoque neo paisajístico de valorización de las dos ciudades moriscas mediante una aprehensión de experiencias compartidas entre los paisajes moriscos del sur y del norte del Mediterráneo, un mini atlas tipológico basado en el método de sky line de geografía de los colores, el método de encuesta por cartografía temática y las herramientas de mediación paisajística. Este enfoque de lectura multiescala nos ha permitido sacar varios hechos, a saber: (i) Discernir los elementos de la construcción natural y de la implantación histórico-espacial de una sociedad hidráulica en ambos sitios. (ii) Diagnosticar las formas de estructuración y de configuración espacial de las diferentes entidades paisajísticas delimitadas y determinar los códigos genéticos del territorio de las dos ciudades moriscas tunecinas. (iii) Identificar un repositorio de lectura multiescala de las mutaciones y transformaciones que caracterizan los paisajes urbanos históricos de Testour y Ghar el Melh. (iv) Discernir los elementos de contraste y diferenciación de los paisajes y tipologías paisajísticas moriscas con respecto a la orilla norte del Mediterráneo. (v) Establecer una guía progresiva de referencia de las entidades paisajísticas inspirada en la ingeniería de los lugares de ambas ciudades. (vi) Establecer un modelo de revalorización y de ecodesarrollo que reconcilie tradiciones y modernidad a través del neopaisajismo y una nueva especialización de los dos paisajes moriscos. En conclusión, esta investigación ha intentado proponer un conjunto de puntos de referencia indispensables para comprender mejor los paisajes hidráulicos moriscos y sus formas de resiliencia y fragilización frente a los desafíos contemporáneos vinculados a las exigencias de desarrollo y a las políticas de ordenación. Palabras clave: paisaje urbano histórico, resiliencia, urbano -ruralidad, fragilización, ecodesarrollo, identidad

New Socio-Spatial Reading of a Remarkable Landscape Located in Testour, toward a Heritage Setting of a Moorish Site

Traditional conservation efforts have not improved conditions in most historic Tunisian cities. However, the changes adopted at the international level in historical urban conservation, based on urban vitality and regions’ sustainability, open horizons to new approaches of reading. Testour, a Moorish city of northern Tunisia, presents one of the historical centers most anchored in identity and the least studied. Our study aims at initiating a new transdisciplinary reading approach that provides a link between time, spaces, actors, culture and sustainability. To do so, we followed a methodological process with a gradual logic that led to a comprehensive management project. This approach presents a set of analytical methodological procedures, which consider historicity in conjunction with a global diagnostic of the landscape in favor of the evaluation of historic centers. This method enabled us to establish a new map showing the structural and functional evolution of Testour throughout history, identifying the valuation indicators and the attributes of global management. This work presents a new reading grid of Testour, including the structural, functional, values and heritage attributes.</jats:p

New Socio-Spatial Reading of a Remarkable Landscape Located in Testour, toward a Heritage Setting of a Moorish Site

Traditional conservation efforts have not improved conditions in most historic Tunisian cities. However, the changes adopted at the international level in historical urban conservation, based on urban vitality and regions’ sustainability, open horizons to new approaches of reading. Testour, a Moorish city of northern Tunisia, presents one of the historical centers most anchored in identity and the least studied. Our study aims at initiating a new transdisciplinary reading approach that provides a link between time, spaces, actors, culture and sustainability. To do so, we followed a methodological process with a gradual logic that led to a comprehensive management project. This approach presents a set of analytical methodological procedures, which consider historicity in conjunction with a global diagnostic of the landscape in favor of the evaluation of historic centers. This method enabled us to establish a new map showing the structural and functional evolution of Testour throughout history, identifying the valuation indicators and the attributes of global management. This work presents a new reading grid of Testour, including the structural, functional, values and heritage attributes

New socio-spatial reading of a remarkable landscape in Testour, towards a heritage of a Moorish place

Traditional conservation efforts have not improved conditions in most historic Tunisian cities. However, the changes adopted at the international level in historical urban conservation, based on urban vitality and regions’ sustainability, open horizons to new approaches of reading. Testour, a Moorish city of northern Tunisia, presents one of the historical centers most anchored in identity and the least studied. Our study aims at initiating a new transdisciplinary reading approach that provides a link between time, spaces, actors, culture and sustainability. To do so, we followed a methodological process with a gradual logic that led to a comprehensive management project. This approach presents a set of analytical methodological procedures, which consider historicity in conjunction with a global diagnostic of the landscape in favor of the evaluation of historic centers. This method enabled us to establish a new map showing the structural and functional evolution of Testour throughout history, identifying the valuation indicators and the attributes of global management. This work presents a new reading grid of Testour, including the structural, functional, values and heritage attributes

Storage and reproductive strategy of the carpet-shell clam, Ruditapes decussatus in the Gulf of Gabès (Tunisia)

International audienc