Verbesserung des Ernährungsverhaltens von 3- bis 6-jährigen Kindern durch verschiedene Interventionsmaßnahmen unter Berücksichtigung sozioökonomischer Faktoren

Ziel der Diplomarbeit und des Projektes MOGI war es fest zu stellen, welche Interventionsmaßnahmen am besten wirken um eine Milch-, Obst-, Gemüseverzehrsteigerung zu erreichen. In Kooperation des Institutes für Ernährungswissenschaften und dem Bundesministerium für Gesundheit wurde das Projekt MOGI (Milch Obst Gemüse Interventionsstudie) durchgeführt. Im Frühjahr des Jahres 2011 konnte nach der Erarbeitung der Interventionsmaßnahmen und Strategien mit dem Projekt gestartet werden. Es gab drei verschiedene Interventionsgruppen, welche unterschiedlichen Maßnahmen zu der Verhaltensänderung und zu der Erhöhung des Ernährungswissen beitragen sollten. Das Setting Kindergarten eignet sich hervorragend, um alle sozialen Schichten zu erreichen. Dabei kann unabhängig des sozialen Status das Ernährungswissen erhöht werden, die Kompetenzen der Kinder fördern und Rahmenbedingungen schaffen die den Kindern ein gesundes Essverhalten erleichtern

Mycoplasma penetrans bacteremia in an immunocompromised patient detected by metagenomic sequencing: a case report.

Background Mycoplasma sp. are well recognized as etiological agents of respiratory and sexually transmitted disease. Mycoplasma penetrans, a species of Mycoplasma sp., has been frequently detected in HIV-positive patients and associated with the progression of HIV-associated disease. To date, there is only a single case report describing M. penetrans as the causative agent of a severe respiratory tract infection in a HIV-negative patient. Case presentation In this report, we describe the case of M. penetrans bacteremia in a HIV-negative, 38-year-old, female, immunocompromised, solid organ transplant patient (combined kidney and pancreas transplantation in 2016), who was admitted to our hospital with anemic uterine bleeding and fever of 38.3 °C. Several hours before her admission at our university hospital, a latex bladder catheter was inserted into her uterus and she complained about fatigue, dizziness and ongoing vaginal bleeding. Laboratory examination showed severe anemia, but microbiological examination was inconspicuous (culture negative vaginal and cervical smears, negative urine culture). Bacterial blood cultures showed a growth signal after 4 h, but microscopic examination with Gram staining and subcultures on different agar media did not identify bacterial pathogens. To identify the bacterial cause of malignancy in the patient, metagenomic sequencing of the blood culture was performed that identified M. penetrans. Conclusion Metagenomic sequencing identified M. penetrans in an immunosuppressed patient with culture-negative bacteremia. Clinicians should be aware of the opportunistic potential of M. penetrans that may cause severe infections in certain vulnerable patient populations and the limitations of culture and Gram staining for confirming the presence of fastidious bacterial pathogens like Mycoplasma spp

Trajectory angles of muscles originating from and inserting to the coxal femoral bone measured on MRIs

Trajectory angles of muscles originating from and inserting to the coxal femoral bone measured on MRIs. Open access supplementary material as appendix to the original article "Muscle forces acting on the greater trochanter lead to a dorsal warping of the apophyseal growth plate" published in Journal of Anatomy

Räumliche Effekte von einzelbaumweise eingemischten Birken auf den Oberboden in einem gekalkten Fichtenbestand im Erz-gebirge

Es wird für einen Fichten-Birkenbestand in den montanen Lagen des Erzgebirges die Wirkung der beiden Baumarten auf die Hu-musform, die mikrobielle Biomasse und ver-schiedene bodenchemische Parameter [pH-Werte, C/N-Verhältnisse, Gesamtgehalte (aus Königswasseraufschluss)] dargestellt. Dabei sind die untersuchten Birken einzelbaumweise in den Fichtenbestand eingemischt. Signifikante Unterschiede treten überwiegend im Of auf. Das ist für den pH, die C-Gehalte und -Vorräte sowie das C/N-Verhältnis der Fall

Saccharomyces cerevisiae from Brazilian kefir-fermented milk: An in vitro evaluation of probiotic properties

The therapeutic use of probiotics for supporting the antibiotic action against gastrointestinal disorders is a current trend and emerging applications have gained popularity because of their support for various microbiological activities in digestive processes. Microorganisms isolated from kefir with great probiotic properties, in addition to high resistance to harsh environmental conditions, have been widely researched. Administration of probiotic yeasts offers a number of advantages, when compared to bacteria, because of particular characteristics as their larger cell size. In the present study, 28 strains of Saccharomyces cerevisiae were isolated, after in vitro digestion of kefir-fermented milk, and identified by molecular based approaches. A screening was performed to determine important quality requirements for probiotics including: antagonistic and antioxidant activities, -galactosidase synthesis, autoaggregation, surface hydrophobicity and adhesion to epithelial cells. The results showed strains: with antagonistic activity against microbial pathogens such as Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis; able to produce -galactosidase; with antioxidant activity levels higher than 90%; with hydrophobicity activity and autoaggregation ability (evaluated by adhesion test, where all the strains presented adhesion to mice ileal epithelial cells). These findings are relevant and the strains are recommended for further in vivo studies as well as for potential therapeutic applications.The authors thank the financial support of CAPES (National Council for the Improvement of Higher Education); the identification of yeast strains by Micoteca URM (Department of Mycology -UFPE); professor Daniela Marques and PhD student Rebeca Melo for providing the mice intestine and the laboratory of Histology (DMFA-UFRPE) for the histological pictures.info:eu-repo/semantics/publishedVersio

Avaliação óssea de regiões maxilares candidatas à reabilitação unitária implantossuportada: uma comunicação curta

Objective: Evaluate the height and bone thickness in healed sites of single implant areas. Materials and Methods: In this cross-sectional study, cone-beam computed tomography (CBCT) images of single edentulous areas of maxilla of patients who needed aesthetic single implant rehabilitations were evaluated for measure the height and thickness using an implant planning software. Data were statistically analyzed using the Mann-Whitney and Pearson correlation test, considering the time, reason and region of tooth loss. For all tests, a p-valueObjetivo: Avaliar a altura e espessura óssea em sítios unitários cicatrizados. Materiais e Métodos: Neste estudo transversal, imagens de tomografia computadorizada de feixe cônico (TCFC) de regiões edêntulas unitárias maxilares de pacientes candidatos a reabilitação unitária implantossuportada foram mensuradas em relação à altura e espessura óssea usando um software de planejamento de implante. Os dados foram analisados estatisticamente por meio do teste Mann-Whitney e de correlação de Pearson, considerando o tempo, o motivo e região da perda dentária. Para todos os testes, um valor de

Bullous Keratopathy in a dog treated with Third Eyelid Flap and Autologous Serum Eye Drops

Background: Bullous Keratopathy (BK) is characterized by decompensation of the corneal stroma resulting in visual impairment by corneal opacity, discomfort, excessive tearing, blepharospasm and pain. The aim of this study was to report a case of BK in a dog and the complete recovery of the ocular structure and visual function, with a third eyelid flap associated with the use of autologous blood serum topically.Case: A 2-year-old Shih Tzu male dog, weighing 4.3 kg, with recurrent bilateral eye discomfort was brought to Ophthalmologist Veterinarian Assistance. The patient had already been seen by other veterinarians, but the signs returned in a short period of time after the prescription was suspended. During physical examination it was observed moderate blepharospasm in the right eye, epiphora in the left eye, and in both eyes was noted exophthalmos, medial corneal entropion, caruncle trichiasis and ectopic cilia in the upper and lower eyelids. Fluorescein test was positive in both eyes, and a large bullous, opaque and gelatinous lesion with irregular appearance was observed occupying a large part of the cornea of the right eye. Superficial corneal ulcer was diagnosed in left eye and BK in right eye, both probably in consequence of ectopic cilia presence. Surgical intervention was made, with the cauterization and excision of the ectopic cilia and third eyelid flap in the right eye. The cauterization of caruncle trichiasis was not authorized by the owner. Moxifloxacin eye drops in the dose of one drop four times a day in both eyes, lubricant based on hyaluronic acid in the dose of one drop four times a day in both eyes, and autogenous blood serum, obtained from the centrifugation of a blood sample of the dog itself, in the dose of one drop every hour in the right eye for 24 hours, and meloxicam in the dose of 0.1 mg/kg every 24 h for 3 days were prescribed. Elizabethan collar was recommended until removal of the third eye flap. During the surgery, a sample of eye secretion was collected and sent for culture and antibiogram and did not show growth of microorganisms. After seven days of the procedure, the animal did not show eye discomfort and corneal ulcer in the left eye was healed. The third eye flap remained stable until the return of 28 days and after removal of the capton, adhesions were identified, which were removed with Castroviejo scissors after application of topical anesthetic proxymetacaine hydrochloride eye drops. A fluorescein test was performed and the absence of corneal keratitis was confirmed. Discrete corneal scars (macula) were identified.Discussion: The patient in this report had several changes in ocular morphology that favored the formation of recurrent injuries which contributed to the formation of BK. The use of topical medications as unique treatment was not performed due to the severity of the lesion and the presence of alterations such as medial entropion, caruncle trichiasis and ectopic cilia that needed surgical corrections to resolve the causes of the lesions. In order to protect the cornea and provide compression of its epithelium, a third eyelid flap was performed, a low-invasive procedure that is quick and easy to perform. Ectopic cilia were cauterized to eliminate one of the causes of the corneal injuries, however, the other changes could not be corrected because they were not authorized by the owner. The adjuvant uses of ocular lubricant based on hyaluronic acid, a broad-spectrum topical antibiotic and mainly autogenous blood serum favored the epithelial reconstruction of the cornea by providing protection, vitamins, growth factors, immunoglobulins, anti-collagenolytic and bacteriostatic substances. The protocol used it enabled a rapid reduction in patient discomfort, reversal of injuries and recovery of visual function

Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics

Burkholderia mallei, the causative agent of glanders, is a clonal descendant of Burkholderia pseudomallei, the causative agent of melioidosis, which has lost its environmental reservoir and has a restricted host range. Despite limitations in terms of sensitivity and specificity, complement fixation is still the official diagnostic test for glanders. Therefore, new tools are needed for diagnostics and to study the B. mallei epidemiology. We recently developed a highly sensitive serodiagnostic microarray test for human melioidosis based on the multiplex detection of B. pseudomallei proteins. In this study, we modified our array tests by using anti-horse IgG conjugate and tested sera from B. mallei-infected horses (n = 30), negative controls (n = 39), and horses infected with other pathogens (n = 14). Our array results show a sensitivity of 96.7% (confidence interval [CI] 85.5 to 99.6%) and a specificity of 100.0% (CI, 95.4 to 100.0%). The reactivity pattern of the positive sera on our array test allowed us to identify a set of 12 highly reactive proteins of interest for glanders diagnosis. The B. mallei variants of the three best protein candidates were selected for the development of a novel dipstick assay. Our point-of-care test detected glanders cases in less than 15 min with a sensitivity of 90.0% (CI, 75.7 to 97.1%) and a specificity of 100.0% (CI, 95.4 to 100.0%). The microarray and dipstick can easily be adopted for the diagnosis of both B. mallei and B. pseudomallei infections in different animals. Future studies will show whether multiplex serological testing has the potential to differentiate between these pathogens