Streamlining Project Development Through Planning & Environmental Linkages

As transportation agencies continue to explore ways to be more efficient in the project development process, they are embracing integrated methodologies like planning and environmental linkages. This tool is practical, yet highly effective and inclusive, and teams across the country are experiencing its value. This presentation will highlight a variety of use cases, discuss lessons learned and best practices, and specifically address how planning and environmental linkages can be applied in Indiana and the Midwest

Improving Document Retention Through Data Management Dashboards

Transportation projects continue to get more complex, requiring multi-layer collaboration, documentation, compliance, and more. Through specific use cases, we will demonstrate how project teams can use innovative platforms to build data management dashboards for project management and document retention for mid-West and Indiana mobility projects. This specialized visual tool brings important documents and data together, incorporates customized information, and evolves it to meet client needs in modern and easy to understand metrics

Cell Cycle-Regulated Protein Abundance Changes in Synchronously Proliferating HeLa Cells Include Regulation of Pre-mRNA Splicing Proteins

Cell proliferation involves dramatic changes in DNA metabolism and cell division, and control of DNA replication, mitosis, and cytokinesis have received the greatest attention in the cell cycle field. To catalogue a wider range of cell cycle-regulated processes, we employed quantitative proteomics of synchronized HeLa cells. We quantified changes in protein abundance as cells actively progress from G1 to S phase and from S to G2 phase. We also describe a cohort of proteins whose abundance changes in response to pharmacological inhibition of the proteasome. Our analysis reveals not only the expected changes in proteins required for DNA replication and mitosis but also cell cycle-associated changes in proteins required for biological processes not known to be cell-cycle regulated. For example, many pre-mRNA alternative splicing proteins are down-regulated in S phase. Comparison of this dataset to several other proteomic datasets sheds light on global mechanisms of cell cycle phase transitions and underscores the importance of both phosphorylation and ubiquitination in cell cycle changes

Challenges and Opportunities for Ecosystem-Based Management and Marine Spatial Planning in the Irish Sea

Ecosystem-Based Management (EBM) integrates the connections between land, air, water and all living things including human beings and their institutions. The location of the Irish Sea, between major historical industrial centres, its history of use and exploitation, combined with its hydrographic characteristics, have led to the current patterns of use. EBM efforts have been ongoing for over a decade but political boundaries have led to fragmented governance. The forthcoming UK exit from the European Union (EU) may pose further challenges. This chapter examines articulations between political boundaries, spatial scales of Marine Spatial Planning and nested social-ecological systems including the gyre in the western Irish Sea, and Dublin Bay. Examples of emerging best practices are provided and the challenges of data availability for ecosystem services are considered

Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins.

Cell proliferation involves dramatic changes in DNA metabolism and cell division, and control of DNA replication, mitosis, and cytokinesis have received the greatest attention in the cell cycle field. To catalogue a wider range of cell cycle-regulated processes, we employed quantitative proteomics of synchronized HeLa cells. We quantified changes in protein abundance as cells actively progress from G1 to S phase and from S to G2 phase. We also describe a cohort of proteins whose abundance changes in response to pharmacological inhibition of the proteasome. Our analysis reveals not only the expected changes in proteins required for DNA replication and mitosis but also cell cycle-associated changes in proteins required for biological processes not known to be cell-cycle regulated. For example, many pre-mRNA alternative splicing proteins are down-regulated in S phase. Comparison of this dataset to several other proteomic datasets sheds light on global mechanisms of cell cycle phase transitions and underscores the importance of both phosphorylation and ubiquitination in cell cycle changes

Cannabinoid receptor Type 1 densities reflect social organization in Microtus.

Across many species, endocannabinoids play an important role in regulating social play, reward, and anxiety. These processes are mediated through at least two distinct cannabinoid receptors (CB), CB1 and CB2. CB1 expression is found in appreciable densities across regions of the brain that integrate memory with socio-spatial information; many of these regions have been directly linked to the neurobiology of pair bonding in monogamous species. Using receptor autoradiography, we provide the first distributional map of CB1 within the brains of closely related monogamous prairie voles and promiscuous meadow voles, and compare receptor densities across sexes and species in limbic regions. We observe CB1-specific signal using [3H] CP-55,940 and [3H] SR141716A, though the latter exhibited a lower signal to noise ratio. We confirmed the presence of CB2 in prairie vole spleen tissue using [3H] CP-55,940. However, we found no evidence of CB2 in the brain using either [3H] CP-55,940 or [3H] A-836,339. The overall distribution of putative CB1 in the brain was similar across vole species and followed the pattern of CB1 expression observed in other species-high intensity binding within the telencephalon, moderate binding within the diencephalon, and mild binding within the mesencephalon and metencephalon (aside from the cerebellar cortex). However, we found profound differences in CB1 densities across species, with prairie voles having higher CB1 binding in regions implicated in social attachment and spatial memory (e.g., periaqueductal gray, hippocampus). These findings suggest that CB1 densities, but not distribution, correlate with the social systems of vole species

Discordance between mRNA and protein abundance.

<p>A) Individual lists of proteins that changed by at least 1.5-fold were compared to the mRNA data for those same proteins in synchronized HeLa cells from Whitfield et al. 2002 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Whitfield1" target="_blank">[7]</a>. The percentage of proteins whose corresponding mRNA also changed is graphed for both S phase and G2 phase. ** p<0.001. B-E) Individual lists of proteins that changed by at least 1.5-fold were compared to proteins predicted to be B) ubiquitinated in asynchronous HCT116 cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Kim1" target="_blank">[15]</a>, C) phosphorylated in HeLa cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Olsen1" target="_blank">[8]</a>, D) substrates of Cyclin A/Cdk2 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Chi1" target="_blank">[17]</a>, and E) substrates of the ATR kinase <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Stokes1" target="_blank">[16]</a>. The percentage of each list that overlaps with the published dataset is plotted. * p<0.01; ** p<0.001.</p

Top three significant GO terms enriched in individual lists of cell cycle-regulated proteins.

<p>Top three significant GO terms enriched in individual lists of cell cycle-regulated proteins.</p

Validation of selected cell cycle-regulated protein predicted by mass spectrometry.

<p>The same cell lysates analyzed by mass spectrometry were subjected to immunoblot analysis for the indicated endogenous proteins in the A) G1 to S lysates or B) S to G2 lysates. Reported fold change ratios from mass spectrometry are listed to the right.</p