Consistently Simulating a Wide Range of Atmospheric Scenarios for K2-18b with a Flexible Radiative Transfer Module

The atmospheres of small, potentially rocky exoplanets are expected to cover a diverse range in composition and mass. Studying such objects therefore requires flexible and wide-ranging modeling capabilities. We present in this work the essential development steps that lead to our flexible radiative transfer module, REDFOX, and validate REDFOX for the Solar system planets Earth, Venus and Mars, as well as for steam atmospheres. REDFOX is a k-distribution model using the correlated-k approach with random overlap method for the calculation of opacities used in the $\delta$-two-stream approximation for radiative transfer. Opacity contributions from Rayleigh scattering, UV / visible cross sections and continua can be added selectively. With the improved capabilities of our new model, we calculate various atmospheric scenarios for K2-18b, a super-Earth / sub-Neptune with $\sim$8 M$_\oplus$ orbiting in the temperate zone around an M-star, with recently observed H$_2$O spectral features in the infrared. We model Earth-like, Venus-like, as well as H$_2$-He primary atmospheres of different Solar metallicity and show resulting climates and spectral characteristics, compared to observed data. Our results suggest that K2-18b has an H$_2$-He atmosphere with limited amounts of H$_2$O and CH$_4$. Results do not support the possibility of K2-18b having a water reservoir directly exposed to the atmosphere, which would reduce atmospheric scale heights, hence too the amplitudes of spectral features inconsistent with the observations. We also performed tests for H$_2$-He atmospheres up to 50 times Solar metallicity, all compatible with the observations.Comment: 28 pages, 13 figures, accepted for publication in Ap

Sensory evaluation and electronic tongue for sensing grafted and non-grafted watermelon taste attributes

The objective of our study was to analyse the results of two measuring methods (sensory evaluation and electronic tongue) and to find differences in taste between grafted and non-grafted watermelon fruit. The trained sensory panel evaluated in two years three differently treated watermelon fruit. The studied fruit samples were produced on the same growing-areas in both years but with different growing technologies. The experiment used the non-grafted/self-rooted watermelon as control sample, while the other two treatments were grafting on two rootstock types: a Lagenaria and an interspecific squash hybrid rootstock. The electronic tongue measurement showed that it is the environment/growing technology that mainly determines the characteristics of the fruit quality, not grafting. The two measurement methods can complement each other in a detailed and practical way, as technology and growing area strongly influence the quality of watermelon fruit. The research also showed that it is possible to have similar watermelon fruit quality, independently from the used rootstock type

Crowdfunding: Geography, Social Networks, and the Timing of Investment Decisions

We examine a crowdfunding platform that connects artists with funders. Although the Internet reduces many distance-related frictions, local and distant funders exhibit different funding patterns. Local funders appear less responsive to information about the cumulative funds raised by an artist. However, this distance effect appears to proxy for a social effect: it is largely explained by funders who likely have an offline social relationship with the artist (“friends and family”). Yet, this social effect does not persist past the first investment, suggesting that it may be driven by an activity like search but not monitoring. Thus, although the platform seems to diminish many distance-sensitive costs, it does not eliminate all of them. These findings provide a deeper understanding of the abilities and limitations of online markets to facilitate transactions and convey information between buyers and sellers with varying degrees of social connectedness

Cross-Disciplinary Genomics Approaches to Studying Emerging Fungal Infections

Emerging fungal pathogens pose a serious, global and growing threat to food supply systems, wild ecosystems, and human health. However, historic chronic underinvestment in their research has resulted in a limited understanding of their epidemiology relative to bacterial and viral pathogens. Therefore, the untargeted nature of genomics and, more widely, -omics approaches is particularly attractive in addressing the threats posed by and illuminating the biology of these pathogens. Typically, research into plant, human and wildlife mycoses have been largely separated, with limited dialogue between disciplines. However, many serious mycoses facing the world today have common traits irrespective of host species, such as plastic genomes; wide host ranges; large population sizes and an ability to persist outside the host. These commonalities mean that -omics approaches that have been productively applied in one sphere and may also provide important insights in others, where these approaches may have historically been underutilised. In this review, we consider the advances made with genomics approaches in the fields of plant pathology, human medicine and wildlife health and the progress made in linking genomes to other -omics datatypes and sets; we identify the current barriers to linking -omics approaches and how these are being underutilised in each field; and we consider how and which -omics methodologies it is most crucial to build capacity for in the near future

Target specificity among canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response

Polyadenylation of pre-mRNAs is critical for efficient nuclear export, stability, and translation of the mature mRNAs, and thus for gene expression. The bulk of pre-mRNAs are processed by canonical nuclear poly(A) polymerase (PAPS). Both vertebrate and higher-plant genomes encode more than one isoform of this enzyme, and these are coexpressed in different tissues. However, in neither case is it known whether the isoforms fulfill different functions or polyadenylate distinct subsets of pre-mRNAs. Here we show that the three canonical nuclear PAPS isoforms in Arabidopsis are functionally specialized owing to their evolutionarily divergent C-terminal domains. A strong loss-of-function mutation in PAPS1 causes a male gametophytic defect, whereas a weak allele leads to reduced leaf growth that results in part from a constitutive pathogen response. By contrast, plants lacking both PAPS2 and PAPS4 function are viable with wild-type leaf growth. Polyadenylation of SMALL AUXIN UP RNA (SAUR) mRNAs depends specifically on PAPS1 function. The resulting reduction in SAUR activity in paps1 mutants contributes to their reduced leaf growth, providing a causal link between polyadenylation of specific pre-mRNAs by a particular PAPS isoform and plant growth. This suggests the existence of an additional layer of regulation in plant and possibly vertebrate gene expression, whereby the relative activities of canonical nuclear PAPS isoforms control de novo synthesized poly(A) tail length and hence expression of specific subsets of mRNAs

Counting a black hole in Lorentzian product triangulations

We take a step toward a nonperturbative gravitational path integral for black-hole geometries by deriving an expression for the expansion rate of null geodesic congruences in the approach of causal dynamical triangulations. We propose to use the integrated expansion rate in building a quantum horizon finder in the sum over spacetime geometries. It takes the form of a counting formula for various types of discrete building blocks which differ in how they focus and defocus light rays. In the course of the derivation, we introduce the concept of a Lorentzian dynamical triangulation of product type, whose applicability goes beyond that of describing black-hole configurations.Comment: 42 pages, 11 figure

Transcriptional analysis of late ripening stages of grapevine berry

* Background: The composition of grapevine berry at harvest is a major determinant of wine quality. Optimal oenological maturity of berries is characterized by a high sugar/acidity ratio, high anthocyanin content in the skin, and low astringency. However, harvest time is still mostly determined empirically, based on crude biochemical composition and berry tasting. In this context, it is interesting to identify genes that are expressed/repressed specifically at the late stages of ripening and which may be used as indicators of maturity. * Results: Whole bunches and berries sorted by density were collected in vineyard on Chardonnay (white cultivar) grapevines for two consecutive years at three stages of ripening (7-days before harvest (TH-7), harvest (TH), and 10-days after harvest (TH+10)). Microvinification and sensory analysis indicate that the quality of the wines made from the whole bunches collected at TH-7, TH and TH+10 differed, TH providing the highest quality wines. In parallel, gene expression was studied with Qiagen/Operon microarrays using two types of samples, i.e. whole bunches and berries sorted by density. Only 12 genes were consistently up-or down-regulated in whole bunches and density sorted berries for the two years studied in Chardonnay. 52 genes were differentially expressed between the TH-7 and TH samples. In order to determine whether these genes followed a similar pattern of expression during the late stages of berry ripening in a red cultivar, nine genes were selected for RT-PCR analysis with Cabernet Sauvignon grown under two different temperature regimes affecting the precocity of ripening. The expression profiles and their relationship to ripening were confirmed in Cabernet Sauvignon for seven genes, encoding a carotenoid cleavage dioxygenase, a galactinol synthase, a late embryogenesis abundant protein, a dirigent-like protein, a histidine kinase receptor, a valencene synthase and a putative S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase. * Conclusions: This set of up-and down-regulated genes characterize the late stages of berry ripening in the two cultivars studied, and are indirectly linked to wine quality. They might be used directly or indirectly to design immunological, biochemical or molecular tools aimed at the determination of optimal ripening in these cultivars

Genome-wide analysis of PAPS1-dependent polyadenylation identifies novel roles for functionally specialized poly(A) polymerases in Arabidopsis thaliana

The poly(A) tail at 3’ ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases,PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying organ growth and suppressing a constitutive immune response. However, the molecular basis of this specialization is largely unknown. Here, we have estimated poly(A)-tail lengths on a transcriptome-wide scale in wild-type and paps1 mutants. This identified categories of genes as particularly strongly affected in paps1 mutants, including genes encoding ribosomal proteins, cell-division factors and major carbohydrate-metabolic proteins. We experimentally verified two novel functions of PAPS1 in ribosome biogenesis and redox homoeostasis that were predicted based on the analysis of poly(A)-tail length changes in paps1 mutants. When overlaying the PAPS1-dependent effects observed here with coexpression analysis based on independent microarray data, the two clusters of transcripts that are most closely coexpressed with PAPS1 show the strongest change in poly(A)-tail length and transcript abundance in paps1 mutants in our analysis. This suggests that their coexpression reflects at least partly the preferential polyadenylation of these transcripts by PAPS1 versus the other two poly(A)-polymerase isoforms. Thus, transcriptome-wide analysis of poly(A)-tail lengths identifies novel biological functions and likely target transcripts for polyadenylation by PAPS1. Data integration with large-scale co-expression data suggests that changes in the relative activities of the isoforms are used as an endogenous mechanism to co-ordinately modulate plant gene expression

Final Report of the AFIT Quality Initiative External Discovery Committee

This report summarizes the findings of the Air Force Institute of Technology’s (AFIT’s) Quality Initiative - External Discovery Team. The overarching purpose of the Quality Initiative is to create a detailed, executable investment strategy for modernizing AFIT’s instructional capabilities across five thrust areas. These activities were completed over the course of one year, beginning in June of 2016 and concluding in June of 2017. The data gathered were evaluated and several recommendations for further review were decided upon by the External Discovery Team. The following report briefly covers those recommendations and provides sources from which the recommendations were gleaned. These recommendations are meant to serve as a baseline for ways in which AFIT could begin to program resources to help improve teaching and instruction across the institution as a whole. The data presented here are meant to serve as a compliment to the Internal Discovery Team’s report that focuses on data and feedback gathered from institutions internal to AFIT