Региональные аспекты исследования сельскохозяйственных предприятий экологической направленности Региональные аспекты исследования сельскохозяйственных предприятий экологической направленности

The article is devoted to the study of the economic phenomenon of eco-settlement in the regional economy. The author's interpretation of the concept of regional economy is introduced, the classification of regional farms according to a number of criteria is carried out. A new definition of eco-settlements is proposed. The classification of modern eco-villages is given. A number of new and traditional eco-settlements have been thoroughly investigated. The importance of eco-settlements for the economy, ecology and society is shown. The study was performed within the research work of the educational-scientific laboratory of regional economy cooperation and integration, agriculture, development of rural territories of the Belgorod state agricultural UNIVERSITY named after V. Gorin, supervisor-associate Professor R. V. Kapinos (in the framework of the agreement No. 6.9.30 of 28 March 2018.) in the framework of the internal grant of Belgorod state agricultural UNIVERSITY (order No. 202-3 from 18.04.19).El artículo está dedicado al estudio del fenómeno económico del eco-asentamiento en la economía regional. Se introduce la interpretación del autor del concepto de economía regional, se lleva a cabo la clasificación de las granjas regionales de acuerdo con una serie de criterios. Se propone una nueva definición de eco-asentamientos. Se da la clasificación de las ecoaldeas modernas. Se han investigado a fondo varios asentamientos ecológicos nuevos y tradicionales. Se muestra la importancia de los eco-asentamientos para la economía, la ecología y la sociedad. El estudio se realizó dentro del trabajo de investigación del laboratorio educativo-científico de cooperación e integración de la economía regional, agricultura, desarrollo de territorios rurales de la UNIVERSIDAD agrícola estatal de Belgorod que lleva el nombre de V. Gorin, profesor supervisor asociado RV Kapinos (en el marco de el acuerdo n. ° 6.9.30 de 28 de marzo de 2018) en el marco de la concesión interna de la UNIVERSIDAD agrícola estatal de Belgorod (orden n. ° 202-3 del 18.04.19). Статья посвящена исследованию экономического феномена экопоселения в экономике региона. Введено авторское толкование понятия региональной экономики, проведена классификация региональных хозяйств по ряду критериев. Предложено новое определение эко-поселений. Дана классификация современных эко-сел. Ряд новых и традиционных эко-поселений были тщательно исследованы. Показана важность эко-поселений для экономики, экологии и общества. Работа выполнена в рамках научно-исследовательской работы учебно-научной лаборатории регионального экономического сотрудничества и интеграции, сельского хозяйства, освоения сельских территорий Белгородского государственного сельскохозяйственного университета им. В. Горина, научного руководителя-доцента Р. В. Капиноса (в рамках договор № 6.9.30 от 28 марта 2018 г.) в рамках внутреннего гранта Белгородского государственного сельскохозяйственного университета (приказ № 202-3 от 18.04.19 г.)

Organelle-specific targeting of polymersomes into the cell nucleus

Synthetic nanomaterials are being sought to shuttle therapeutic payloads directly into the cell nucleus as a major target for chemo- and gene-based therapies. However, it remains uncertain whether and how synthetic entities are able to bypass the nuclear pore complexes (NPCs) that regulate transport into and out of the nucleus. We have constructed biocompatible polymer vesicles that infiltrate NPCs and resolved their nuclear uptake mechanism in vitro and in vivo. Their ability to deliver payloads directly into cell nuclei is further validated by transmission electron microscopy.Organelle-specific nanocarriers (NCs) are highly sought after for delivering therapeutic agents into the cell nucleus. This necessitates nucleocytoplasmic transport (NCT) to bypass nuclear pore complexes (NPCs). However, little is known as to how comparably large NCs infiltrate this vital intracellular barrier to enter the nuclear interior. Here, we developed nuclear localization signal (NLS)-conjugated polymersome nanocarriers (NLS-NCs) and studied the NCT mechanism underlying their selective nuclear uptake. Detailed chemical, biophysical, and cellular analyses show that karyopherin receptors are required to authenticate, bind, and escort NLS-NCs through NPCs while Ran guanosine triphosphate (RanGTP) promotes their release from NPCs into the nuclear interior. Ultrastructural analysis by regressive staining transmission electron microscopy further resolves the NLS-NCs on transit in NPCs and inside the nucleus. By elucidating their ability to utilize NCT, these findings demonstrate the efficacy of polymersomes to deliver encapsulated payloads directly into cell nuclei

Dynamic molecular mechanism of the nuclear pore complex permeability barrier

Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport of specific macromolecules while impeding the exchange of unsolicited material. However, key aspects of this gating mechanism remain controversial. To address this issue, we determined the nanoscopic behavior of the permeability barrier directly within yeast S. cerevisiae NPCs at transport-relevant timescales. We show that the large intrinsically disordered domains of phenylalanine-glycine repeat nucleoporins (FG Nups) exhibit highly dynamic fluctuations to create transient voids in the permeability barrier that continuously shape-shift and reseal, resembling a radial polymer brush. Together with cargo-carrying transport factors the FG domains form a feature called the central plug, which is also highly dynamic. Remarkably, NPC mutants with longer FG domains show interweaving meshwork-like behavior that attenuates nucleocytoplasmic transport in vivo. Importantly, the bona fide nanoscale NPC behaviors and morphologies are not recapitulated by in vitro FG domain hydrogels. NPCs also exclude self-assembling FG domain condensates in vivo, thereby indicating that the permeability barrier is not generated by a self-assembling phase condensate, but rather is largely a polymer brush, organized by the NPC scaffold, whose dynamic gating selectivity is strongly enhanced by the presence of transport factors.</p

Farnesylated Nuclear Proteins Kugelkern and Lamin Dm0 Affect Nuclear Morphology by Directly Interacting with the Nuclear Membrane

Nuclear shape changes are observed during a variety of developmental processes, pathological conditions and ageing. Here, the molecular mechanism is analyzed how the farnesylated nuclear proteins interact with the nuclear envelope and deform the phospholipid bilayer

Classification of the nucleolytic ribozymes based upon catalytic mechanism

The nucleolytic ribozymes carry out site-specific RNA cleavage reactions by nucleophilic attack of the 2’-oxygen atom on the adjacent phosphorus with an acceleration of a million-fold or greater. A major part of this arises from concerted general acid-base catalysis. Recent identification of new ribozymes has expanded the group to a total of nine and this provides a new opportunity to identify sub-groupings according to the nature of the general base and acid. These include nucleobases, hydrated metal ions, and 2’-hydroxyl groups. Evolution has selected a number of different combinations of these elements that lead to efficient catalysis. These differences provide a new mechanistic basis for classifying these ribozymes

Search for neutral charmless B decays at LEP

A search for rare charmless decays of \Bd and \Bs mesons has been performed in the exclusive channels \Bd_{(\mathrm s)}\ra\eta\eta, \Bd_{(\mathrm s)}\ra\eta\pio and \Bd_{(\mathrm s)}\ra\pio\pio. The data sample consisted of three million hadronic \Zo decays collected by the L3 experiment at LEP from 1991 through 1994. No candidate event has been observed and the following upper limits at 90\% confidence level on the branching ratios have been set \begin{displaymath} \mathrm{Br}(\Bd\ra\eta\eta)<4.1\times 10^{-4},\,\, \mathrm{Br}(\Bs\ra\eta\eta)<1.5\times 10^{-3},\,\, \end{displaymath} \begin{displaymath} \mathrm{Br}(\Bd\ra\eta\pio)<2.5\times 10^{-4},\,\, \mathrm{Br}(\Bs\ra\eta\pio)<1.0\times 10^{-3},\,\, \end{displaymath} \begin{displaymath} \mathrm{Br}(\Bd\ra\pio\pio)<6.0\times 10^{-5},\,\, \mathrm{Br}(\Bs\ra\pio\pio)<2.1\times 10^{-4}. \end{displaymath} These are the first experimental limits on \Bd\ra\eta\eta and on the \Bs neutral charmless modes

Measurement of the lifetime of the τ\tau lepton

The lifetime of the tau lepton is measured using data collected in 1994 by the L3 detector at LEP. The precise track position information of the Silicon Microvertex Detector is exploited. The tau lepton lifetime is determined from the signed impact parameter distribution for 30 322 tau decays into one charged particle and from the decay length distribution for 3891 tau decays into three charged particles. Combining the two methods we obtain $\tau_{\tau}$ = 290.1 $\pm$ 4.0 fs