The effects of different dietary levels of organic and inorganic selenium on some growth performance and proximate composition of juvenile rainbow trout (Oncorhynchus mykiss)

Selenium, a trace mineral complement is used as cofactor of antioxidant enzymes that protects fishes against environmental stress agents and enhances performance in fishes. In this study the different levels of organic and inorganic source of selenium were included in juvenile rainbow trout diet to evaluate feed conversion ratio (FCR), specific growth rate (SGR), weight gain percent (WG), condition factor (CF), survival rate (SR) and proximate analysis of the fillet during 60 days of the experiment. The fishes were allotted to 9 treatment groups including: Tc the fishes were fed diet without any selenium, control group; To1, To2, To3 and To4 the fishes were fed different dosages of inorganic [sic] selenium; and Ti1, Ti2, Ti3 and Ti4 those were fed different dose of inorganic Se in their diet. Results showed that To4, showed the highest level of WG from 50±2.8 to 168.54± 25.56 g in comparison to Tc (134.38± 27.26g) (p<0.05). Average initial total length of fishes (19± 1.12 centimeters) increased to 21.1± 1.12 cm in Tc and 22.46± 1.25 cm in To4 significantly (p<0.05). Among all treatments, FCR, SGR, WG, CF and SR were improved in To3 group. Also carcass protein increased in To4 (32.58±1.22%) on the contrary of Ti4 (22.43±1.51%) (p<0.05). As a general conclusion, dietary incorporation of organic selenium at 0.45 mg/kg showed satisfactory results in some growth parameters and was a useful supplement in salmonid fish diets

Identification de génotypes-S chez les descendances d’amandiers par les méthodes NEPHGE et PCR

Almond (P. amygdalus Batsch) shows a gametophytic self-incompatibility system controlled by a multiallelic locus, known as the locus S. Self-compatibility has been related to Sf allele presence and this trait has become a priority in the main almond breeding programs and the search for new cultivars is focused on the evaluation of desirable traits in autogamous seedlings. Traditionally, self-compatibility has been assessed in almond by laborious and time consuming methods, such as determination of fruit set in bagged branches or microscopic observation of pollen tube growth after self-pollination in laboratory conditions. Recently, molecular methods have been developed to assess the S-genotype, such as the identification of stylar S-RNases by NEPHGE (non equilibrium pH gradient electro focusing) and the use of conserved and specific PCR primers for the amplification of fragments from the different S alleles. In this research we applied these molecular methods for the S-genotype assessment in two almond breeding progenies, from the crosses of the self-compatible elite selection 'G-2-25' (S11Sf) of the CITA, as female parent, with two self-incompatible cultivars, 'Desmayo Largueta' (S1S25) and 'Marcona' (S11S12), as male parents. Although no discrepancies were found between the two methods, PCR was more suitable than NEPHGE for S-genotype assessment. PCR is easier to optimize, cheaper, more precise and reliable. It is also possible to assess the genotype sooner than with NEPHGE, as flowers are not required for the determination, allowing an earlier elimination of the seedlings. In the 'G-2-25' (S11Sf) x 'Marcona' (S11S12) progeny, the ratio of genotypes was 42% S11S12 and 58% SfS12, approaching the Mendelian laws of transmission in spite of the slightly higher proportion of self-compatible seedlings. In the other family, 'G-2-25' (S11Sf) x 'Desmayo Largueta' (S1S25), four S-genotypes are possible, but the ratios obtained were 21% S1S11, 53% S1Sf, 0% S11S25, and 26% SfS25. These results showed that pollen carrying the S25 allele had only a 26% fertilization success as compared to 73 of pollen carrying the S1 allele. The absence of S11S25 seedlings may imply the expression of a homozygous lethal trait in these zygotes and the distortion of the Mendelian ratiosL’amandier (P. amygdalus Batsch) montre un système d’auto-incompatibilité gamétophytique contrôlé par un locus multiallélique, connu comme locus S. L’auto-compatibilité a été reliée à la présence de l’allèle Sf et cette caractéristique est devenue une priorité pour les principaux programmes d’amélioration de l’amandier, et ainsi la recherche de nouveaux cultivars se focalise sur l’évaluation des caractères désirables pour les plants autogames. Traditionnellement, l’auto-compatibilité a été évaluée chez les amandiers par des méthodes laborieuses et prenant beaucoup de temps, telles que la détermination de la nouaison pour les branches ensachées ou l’observation microscopique de la croissance des tubes de pollen après auto-pollinisation en conditions de laboratoire. Récemment, des méthodes moléculaires ont été développées pour évaluer le génotype-S, telles que l’identification de S-RNases stylaires par NEPHGE (non equilibrium pH gradient electro-focusing) et l’utilisation d’amorces PCR conservées et spécifiques pour l’amplification de fragments provenant de différents allèles-S. Pour cette recherche nous avons appliqué ces méthodes moléculaires pour l’évaluation du génotype-S chez les descendances améliorées de deux amandiers, à partir des croisements d’une sélection d’élite auto-compatible 'G-2-25' (S11Sf) du CITA, comme parent femelle, avec deux cultivars auto-incompatibles, 'Desmayo Largueta' (S1S25) et 'Marcona' (S11S12), comme parents mâles. Bien que l’on n’ait pas trouvé de divergences entre les deux méthodes, la PCR était plus adéquate que NEPHGE pour l’évaluation du génotype-S. La PCR est plus facile à optimiser, moins onéreuse, plus précise et fiable. Il est également possible d’évaluer le génotype plus tôt qu’avec NEPHGE, étant donné qu’il n’est pas nécessaire d’obtenir des fleurs pour la détermination, permettant ainsi une élimination précoce des plants. Dans la descendance de 'G-2-25' (S11Sf) 'Marcona' (S11S12), le quotient des génotypes était de 42% S11S12 et de 58% SfS12, se rapprochant des lois mendéliennes de transmission malgré la proportion légèrement plus élevée de plants auto-compatibles. Pour l’autre famille, 'G-2-25' (S11Sf) 'Desmayo Largueta' (S1S25), quatre génotypes-S sont possibles, mais les quotients obtenus étaient de 21% S1S11, 53% S1Sf, 0% S11S25, et 26% SfS25. Ces résultats montrent que le pollen portant l’allèle S25 avait une réussite de fertilisation d’uniquement 26% comparée à 73 pour le pollen portant l'allèle S1. L’absence de plants S11S25 pourrait faire penser à l’expression d’un caractère létal homozygote chez ces zygotes et à la distortion des quotients mendélien

Effects of organophosphate, diazinon on some haematological and biochemical changes in Rutilus frisii kutum (Kamensky, 1901) male brood stocks

The acute toxicity and effects of diazinon on some haematological parameters of kutum (Rutilus frisii kutum, Kamensky, 1901) weighing 613.33 ± 157.06 g were studied under static water quality conditions at 15 °C ± 2 ºC. These experiments were carried out based on the standard TRC, 1984 method over 4 days, and controlled for the effective water physicochemical factors having pH ranges of 7-8.2, total hardness 300 mgl^-1 (CaCO3), dissolved oxygen 7 mgl^-1. The results showed that the 96h LC50 value of diazinon was 0.4 mgl^-1 and that the maximum allowable concentration (MAC) value of this toxin was 0.04 mgl^-1. The second stage of experiments consisted of four treatments: LC0: 0 as blank, treatment A with a concentration of LC1: 0.107 mgl^-1, treatment B with a concentration of LC5: 0.157 mgl^-1, treatment C with a concentration of MAC value: 0.04 mgl^-1. Male brood stocks of R. frisii were treated with these concentrations for 45 days. The results showed that long-term exposure to diazinon causes a decrease in the erythrocyte count (RBC), haemoglobin (Hb), haematocrit (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), leucocyte count (WBC), lymphocyte, testosterone, iron (Fe), sodium (Na), lactate dehydrogenase (LDH) and cholinesterase (CHeS) (P0.05). The results showed that long-term exposure to low concentrations of diazinon causes changes in some haematological and biochemical parameters of R. frisii male brood stocks

Long-term field and laboratory leaching tests of cemented radioactive wastes

Experiments with real and simulated radioactive cementitious wasteforms were set up to compare the leaching behaviour of cementitious wasteforms containing nuclear power plant operational waste in field and laboratory test conditions. Experiments revealed that the average annual Cs-137 leach rate in deionised water was about thirty-five times greater compared with the measured average value for the 1st year of the field test. Cumulative leached fraction of Cs-137 for 1st year (3.74%) was close to values reported in literature for similar laboratory experiments in deionised water, however more than two orders of magnitude higher than the 1st year leached fraction of Cs-137 in the repository test (0.01%). Therefore, to compare field and laboratory test results, a scaling factor is required in order to account for surface to volume factor difference, multiplied by a temperature factor and a leach rate decrease coefficient related to the ground water composition. (C) 2011 Elsevier B.V. All rights reserved

PTPN22 gene polymorphism in Takayasu's arteritis

Objective. Takayasu's arteritis (TA) is a chronic, rare granulomatous panarteritis of unknown aetiology involving mainly the aorta and its major branches. In this study, genetic susceptibility to TA has been investigated by screening the functional single nucleotide polymorphism (SNP) of PTPN22 gene encoding the lymphoid-specific protein tyrosine phosphatase. Methods. Totally, 181 patients with TA and 177 healthy controls are genotyped by PCR-RFLP method for the SNP rs2476601 (A/G) of PTPN22 gene. Polymorphic region was amplified by PCR and digested with Xcm I enzyme. Results. Detected frequencies of heterozygous genotype (AG) were 5.1% (9/177) in control group and 3.8% (7/181) in TA group (P = 0.61, odds ratio: 0.75, 95% CI: 0.3, 2.0). No association with angiographic type, vascular involvement or prognosis of TA was observed either. Conclusion. The distribution of PTPN22 polymorphism did not reveal any association with TA in Turkey. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved

Эффективность привлечения иностранной рабочей силы и их вклад в возрождение экономики Йемена

In this paper, we define the amount of trade and commercial contacts between Yemen and Turkey from 2015 to 2018 as an example of the number of trade exchanges that Yemen wishes to send its products to more than one nation in the future, including Belarus, the Russian Federation, and others.В качестве примера определен объем торговли и коммерческих контактов между Йеменом и Турцией с 2015 по 2018 г. Также Йемен намерен отправить свою продукцию в несколько стран в будущем, включая Беларусь, Российскую Федерацию и др

A Survey on Retrieval of Mathematical Knowledge

We present a short survey of the literature on indexing and retrieval of mathematical knowledge, with pointers to 72 papers and tentative taxonomies of both retrieval problems and recurring techniques.Comment: CICM 2015, 20 page

Galilean Conformal Algebra in Semi-Infinite Space

In the present work we considered Galilean conformal algebras (GCA), which arises as a contraction relativistic conformal algebras ($x_i\rightarrow \epsilon x_i$, $t\rightarrow t$, $\epsilon \rightarrow 0$). We can use the Galilean conformal symmetry to constrain two-point and three-point functions. Correlation functions in space-time without boundary condition were found in \cite{1}. In real situations there are boundary conditions in space-time, so we have calculated correlation functions for Galilean confrormal invariant fields in semi-infinite space with boundary condition in $r=0$. We have calculated two-point and three-point functions with boundary condition in fixed time.Comment: 14 page

Recognitionand Analysis of Microstructure Parameters of Porous Anodic Films Using ImageJ

The most important parameters that characterize the microstructure of the films and determine the possibility of their use as porous templates are the pore diameter, porosity, and ordering of the porous structure. Therefore, to increase the efficiency of the use of porous anodic alumina films, it is important to investigate the effect of the formation modes on the microstructure. The aim of this work was to choose and optimize a model for processing experimental data obtained by scanning electron microscopy in the ImageJ to determine the parameters of the microstructure of porous films. The work shows the result of SEMimage analysis and obtains plots of pore diameter distribution by size and determines the diameter of the main pores