Deep levels in homoepitaxial boron-doped diamond films studied by capacitance transient spectroscopies

International audienceDeep level transient spectroscopies (DLTS) applied to Schottky junctions made on homoepitaxial boron-doped diamond films show the existence of two traps. A deep acceptor, negatively charged and strongly attractive for holes, 1.57 eV above the valence band edge displays the characteristic features of a complex defect due to interacting centers and impurities, also displaying some evolutions after thermal cycles, possibly due to hydrogen effusion or diffusion. It is tentatively ascribed to association of a boron atom, a vacancy and several hydrogen atoms. A deep donor, 1.13 eV above the valence band edge, able to compensate the boron acceptors, is attributed to a defect correlated with dislocations. It could be due to the positively charged carbon vacancy. These conclusions are drawn from the Fourier transform-DLTS results coupled with isothermal time domain algorithms allowing the discrimination of multiple emission rates with high resolution

Nonequilibrium transport in quantum impurity models: Exact path integral simulations

We simulate the nonequilibrium dynamics of two generic many-body quantum impurity models by employing the recently developed iterative influence-functional path integral method [Phys. Rev. B {\bf 82}, 205323 (2010)]. This general approach is presented here in the context of quantum transport in molecular electronic junctions. Models of particular interest include the single impurity Anderson model and the related spinless two-state Anderson dot. In both cases we study the time evolution of the dot occupation and the current characteristics at finite temperature. A comparison to mean-field results is presented, when applicable

Processing of triplex-directed psoralen DNA interstrand crosslinks by recombination mechanisms

Gene targeting via homologous recombination (HR) is an important application in biotechnology and medicine. However, in mammalian cells HR is much less efficient than random integration. Triplex-forming oligonucleotides (TFOs) linked to DNA damaging agents (e.g. psoralen) can stimulate HR, providing the potential to improve gene therapy applications. To elucidate factors affecting TFO-directed psoralen interstrand crosslink (ICL)-induced recombination, we constructed a series of plasmids with duplicated supF reporter genes, each containing an inactivating deletion, to measure HR frequencies in mammalian cells. Our results indicated that TFO-directed ICL-induced recombination frequencies were higher in the plasmids with larger distances between duplicated supF genes than with a smaller separation distance. However, the position of the ICL relative to the reporter genes did not affect HR frequencies. Recombination spectra were altered by the distance between supF copies. Although single-strand annealing (SSA) recombinants were predominant in all plasmid substrates, the plasmid with the shortest interval (60 bp) revealed a significant proportion of gene conversions (GCs). GCs occurred exclusively in the gene containing the shortest deletion, regardless of the distance between supF genes, ICL position or deletion orientation. Our analyses indicated that SSA is the predominant mechanism of ICL processing of these substrates in mammalian cells

Summation of response rates to discriminative stimuli associated with qualitatively different reinforcers

In Experiment I, a four-ply multiple schedule was used to study the effects on rate of responding in rats of food, water, and food and/or water reinforcement under different deprivation conditions. Food and water were associated separately with different stimuli, the combination of which was associated with food and water together, or with food or water randomly. Rates in the presence of the combined stimuli were consistently intermediate to the rates generated by the separate stimuli, a result seemingly incompatible with a “summation” hypothesis. Experiment II was a simplified systematic replication of Experiment I, verifying the major findings

Combined-stimulus control as a function of the response rate controlled by the absence of the single stimuli

Rat's bar-press responses were maintained at moderate rates during separate presentations of light and tone by separate but concurrent variable-interval schedules of food and shock presentation. The relative response rate maintained during light-out-no-tone was alternated in four successive phases: in Phases 1 and 3 responding was maintained at a higher rate than that during light and tone alone by a variable-interval food schedule, while in Phases 2 and 4 responding was reduced to a lower rate by a differential-reinforcement-of-other-behavior food schedule. In test presentations of light, tone and a light-plus-tone combination, administered at the end of each phase, the proportion of responses emitted during light-plus-tone was an inverse function of the relative response rate controlled by light-out-no-tone, indicating that the relative training response rate controlled by the absence of the single stimuli determined the control exerted by the combined stimuli. Different relative response rates maintained in training may also be partly responsible for previously observed differences in the form of generalization gradients following the establishment of multi-stimulus control

Predictors of Mucoid Pseudomonas Colonization in Cystic Fibrosis Patients

Rationale: Chronic mucoid Pseudomonas aeruginosa within the airway in cystic fibrosis (CF) patients can determine prognosis. Understanding the risk factors of mucoid P. aeruginosa acquisition may change how we deliver care. This study aims to evaluate whether presence of risk factors reported to predict disease severity including gender, CFTR genotype, bacterial organisms in airway cultures, and serum levels of vitamins A and E, albumin, C-reactive protein, alpha 1-antitrypsin, and immunoglobulins increased the risk of mucoid P. aeruginosa acquisition. Methods: Primary endpoint was age at first transition from negative to positive culture for mucoid P . aeruginosa . Cox proportional hazards regression with time-dependent covariates examined development of mucoid P. aeruginosa infection and its association with longitudinally measured serum biomarkers, pulmonary function, and culture results for other organisms. Results: Median ages at CF diagnosis and at first culture were 0.55 and 5.7 years, respectively. Median number of cultures/patient was 17. Of the 323 subjects, 150 developed mucoid P. aeruginosa during a median 8.1 years’ follow-up. In multivariate analysis, gender (relative hazard [RH] 0.55 for male vs. female, P =0.001), number of DF508 alleles (RH 1.66 for1 or 2 vs. 0, P =0.04), FEV1 % (RH 1.16 for 10% decrease, P=0.008), and most recent Staphylococcus aureus status (RH 0.24 for positive vs. negative, P < 0.0001) remained statistically significant. Conclusion: Female gender, number of DF508 alleles, decreased lung function, and lack of S. aureus on recent sputum culture are important risk factors for early detection of mucoid P . aeruginosa