Design and investigation of high-speed, large-force and long-lifetime electromagnetic actuators by finite element modelling

Electromagnetic (EM) solenoid actuators are widely used in many applications such as the automobile, aerospace, printing and food industries where repetitive, often high-speed linear or rotating motions are required. In some of these applications they are used as highspeed `switching' valves for switching pneumatic channels. This paper describes the finite element (FE) modelling and design of high-speed solenoid actuators. Operating at frequencies between 150–300 Hz, these actuators are unique in terms of the large force they produce (8–15 N) and the requirement for very long lifetime (2-5 billion cycles). The complex nature of electromagnetic, motional and thermal problems is discussed. The methodologies for FE modelling of such high-performance actuators are developed and discussed. These are used for modelling, design, performance evaluation and prediction of the above high-speed actuators. Modelling results showing some of the key design features of the actuators are presented in terms of force produced as a function of various design parameters

Open Challenges in Vetting the Internet-of-Things

Internet-of-Thing (IoT) is a rapid-emerging technology that exploits the concept of inter-network to connect things such as physical devices and objects together. A huge number of things (6.4 billion are in use in 2016) are already acting without direct human control raising a lot of concerns about the readiness and appropriateness of existing security practices, techniques, and tools to secure the data collected and protect people’s private lives. As a first step, this paper presses the importance of having a dedicated process for vetting IoT (by analogy to vetting mobile apps) with focus on exposing things’ vulnerabilities that could be the primary source of attacks. These vulnerabilities are identified according to things’ duties decomposed into sensing, actuating, and communicating. A set of questions shed light on things’ vulnerabilities per type of duty

Enterprise 2.0: Research challenges and opportunities

© Springer International Publishing Switzerland 2015. Blending Web 2.0 technologies with enterprise information systems is setting up the stage for a new generation of information systems that will help enterprises open up new communication channels with their stakeholders. Contrary to traditional enterprises with top-down command flow and bottom-up feedback flow, the same flows in Enterprise 2.0 cross all levels and in all directions bringing people together in the development of creative and innovative ideas. The power of Web 2.0 technologies stems from their ability to capture real-world phenomena such as collaboration, competition, and partnership that can be converted into useful and structured information sources from which enterprises can draw information about markets’ trends, consumers’ habits, suppliers’ strategies, etc. This paper discusses the research efforts that our international research group has put into the topic of Enterprise 2.0 (aka Social Enterprise). In particular, our research group advocates that existing practices for managing enterprise information systems need to be re-visited in a way that permits to capture social relations that arise inside and outside the enterprise, to establish guidelines and techniques to assist IT practitioners integrate social relations into their design, development, and maintenance efforts of these information systems, and last but not least to identify and tackle challenges that prevent capturing social relations

Spag17 Deficiency Results in Skeletal Malformations and Bone Abnormalities

Height is the result of many growth and development processes. Most of the genes associated with height are known to play a role in skeletal development. Single-nucleotide polymorphisms in the SPAG17 gene have been associated with human height. However, it is not clear how this gene influences linear growth. Here we show that a targeted mutation in Spag17 leads to skeletal malformations. Hind limb length in mutants was significantly shorter than in wild-type mice. Studies revealed differences in maturation of femur and tibia suggesting alterations in limb patterning. Morphometric studies showed increased bone formation evidenced by increased trabecular bone area and the ratio of bone area to total area, leading to reductions in the ratio of marrow area/total area in the femur. Micro-CTs and von Kossa staining demonstrated increased mineral in the femur. Moreover, osteocalcin and osterix were more highly expressed in mutant mice than in wild-type mice femurs. These data suggest that femur bone shortening may be due to premature ossification. On the other hand, tibias appear to be shorter due to a delay in cartilage and bone development. Morphometric studies showed reduction in growth plate and bone formation. These defects did not affect bone mineralization, although the volume of primary bone and levels of osteocalcin and osterix were higher. Other skeletal malformations were observed including fused sternebrae, reduced mineralization in the skull, medial and metacarpal phalanges. Primary cilia from chondrocytes, osteoblasts, and embryonic fibroblasts (MEFs) isolated from knockout mice were shorter and fewer cells had primary cilia in comparison to cells from wild-type mice. In addition, Spag17 knockdown in wild-type MEFs by Spag17 siRNA duplex reproduced the shorter primary cilia phenotype. Our findings disclosed unexpected functions for Spag17 in the regulation of skeletal growth and mineralization, perhaps because of its role in primary cilia of chondrocytes and osteoblasts

179^{179}Ta(n, γ) cross-section measurement and the astrophysical origin of the 180^{180}Ta isotope

$^{180m}$Ta is nature\u27s rarest (quasi) stable isotope and its astrophysical origin is an open question. A possible production site of this isotope is the slow neutron capture process in asymptotic giant branch stars, where it can be produced via neutron capture reactions on unstable $^{179}$Ta. We report a new measurement of the $^{179}$Ta(n,γ) $^{180}$Ta cross section at thermal-neutron energies via the activation technique. Our results for the thermal and resonance-integral cross sections are 952±57 and 2013±148 b, respectively. The thermal cross section is in good agreement with the only previous measurement [Phys. Rev. C 60, 025802 (1999)], while the resonance integral is different by a factor of ≈1.7. While neutron energies in this work are smaller than the energies in a stellar environment, our results may lead to improvements in theoretical predictions of the stellar cross section

A characteristics framework for Semantic Information Systems Standards

Semantic Information Systems (IS) Standards play a critical role in the development of the networked economy. While their importance is undoubted by all stakeholders—such as businesses, policy makers, researchers, developers—the current state of research leaves a number of questions unaddressed. Terminological confusion exists around the notions of “business semantics”, “business-to-business interoperability”, and “interoperability standards” amongst others. And, moreover, a comprehensive understanding about the characteristics of Semantic IS Standards is missing. The paper addresses this gap in literature by developing a characteristics framework for Semantic IS Standards. Two case studies are used to check the applicability of the framework in a “real-life” context. The framework lays the foundation for future research in an important field of the IS discipline and supports practitioners in their efforts to analyze, compare, and evaluate Semantic IS Standard

Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Background: While most resources in biofuels were directed towards implementing bioethanol programs, 1-propanol has recently received attention as a promising alternative biofuel. Nevertheless, no microorganism has been identified as a natural 1-propanol producer. In this study, we manipulated a novel metabolic pathway for the synthesis of 1-propanol in the genetically tractable bacterium Escherichia coli. Results: E. coli strains capable of producing heterologous 1-propanol were engineered by extending the dissimilation of succinate via propionyl-CoA. This was accomplished by expressing a selection of key genes, i.e. (1) three native genes in the sleeping beauty mutase (Sbm) operon, i.e. sbm-ygfD-ygfG from E. coli, (2) the genes encoding bifunctional aldehyde/alcohol dehydrogenases (ADHs) from several microbial sources, and (3) the sucCD gene encoding succinyl-CoA synthetase from E. coli. Using the developed whole-cell biocatalyst under anaerobic conditions, production titers up to 150 mg/L of 1-propanol were obtained. In addition, several genetic and chemical effects on the production of 1-propanol were investigated, indicating that certain host-gene deletions could abolish 1-propanol production as well as that the expression of a putative protein kinase (encoded by ygfD/argK) was crucial for 1-propanol biosynthesis. Conclusions: The study has provided a novel route for 1-propanol production in E. coli, which is subjected to further improvement by identifying limiting conversion steps, shifting major carbon flux to the productive pathway, and optimizing gene expression and culture conditions.Natural Sciences and Engineering Research Council (NSERC); Canada Research Chair (CRC) program of Canad

Quick Ultra-VIolet Kilonova surveyor (QUVIK)

We present a near-UV space telescope on a ~70kg micro-satellite with a moderately fast repointing capability and a near real-time alert communication system that has been proposed in response to a call for an ambitious Czech national mission. The mission, which has recently been approved for Phase 0, A, and B1 study shall measure the brightness evolution of kilonovae, resulting from mergers of neutron stars in the near-UV band and thus it shall distinguish between different explosion scenarios. Between the observations of transient sources, the satellite shall perform observations of other targets of interest, a large part of which will be chosen in open competition.Comment: SPIE Astronomical Telescopes and Instrumentatio

Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation

The universally conserved translation elongation factor EF-Tu delivers aminoacyl(aa)-tRNA in the form of an aa-tRNA·EF-Tu·GTP ternary complex (TC) to the ribosome where it binds to the cognate mRNA codon within the ribosomal A-site, leading to formation of a pretranslocation (PRE) complex. Here we describe preparation of QSY9 and Cy5 derivatives of the variant E348C-EF-Tu that are functional in translation elongation. Together with fluorophore derivatives of aa-tRNA and of ribosomal protein L11, located within the GTPase associated center (GAC), these labeled EF-Tus allow development of two new FRET assays that permit the dynamics of distance changes between EF-Tu and both L11 (Tu-L11 assay) and aa-tRNA (Tu-tRNA assay) to be determined during the decoding process. We use these assays to examine: (i) the relative rates of EF-Tu movement away from the GAC and from aa-tRNA during decoding, (ii) the effects of the misreading-inducing antibiotics streptomycin and paromomycin on tRNA selection at the A-site, and (iii) how strengthening the binding of aa-tRNA to EF-Tu affects the rate of EF-Tu movement away from L11 on the ribosome. These FRET assays have the potential to be adapted for high throughput screening of ribosomal antibiotics

Homocysteinylated Albumin Promotes Increased Monocyte-Endothelial Cell Adhesion and Up-Regulation of MCP1, Hsp60 and ADAM17

RATIONALE:The cardiovascular risk factor homocysteine is mainly bound to proteins in human plasma, and it has been hypothesized that homocysteinylated proteins are important mediators of the toxic effects of hyperhomocysteinemia. It has been recently demonstrated that homocysteinylated proteins are elevated in hemodialysis patients, a high cardiovascular risk population, and that homocysteinylated albumin shows altered properties. OBJECTIVE:Aim of this work was to investigate the effects of homocysteinylated albumin - the circulating form of this amino acid, utilized at the concentration present in uremia - on monocyte adhesion to a human endothelial cell culture monolayer and the relevant molecular changes induced at both cell levels. METHODS AND RESULTS:Treated endothelial cells showed a significant increase in monocyte adhesion. Endothelial cells showed after treatment a significant, specific and time-dependent increase in ICAM1 and VCAM1. Expression profiling and real time PCR, as well as protein analysis, showed an increase in the expression of genes encoding for chemokines/cytokines regulating the adhesion process and mediators of vascular remodeling (ADAM17, MCP1, and Hsp60). The mature form of ADAM17 was also increased as well as Tnf-α released in the cell medium. At monocyte level, treatment induced up-regulation of ICAM1, MCP1 and its receptor CCR2. CONCLUSIONS:Treatment with homocysteinylated albumin specifically increases monocyte adhesion to endothelial cells through up-regulation of effectors involved in vascular remodeling