Nutri-concept : Innovative food concepts and technologies for global nutrition and business

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Genomic methods in analyzing the communities of soil bacteria

Culture-independent examination of complex microbial communities has been made possible by recent advances in molecular biology. Terminal restriction fragment length polymorphism is one of such techniques that allow rapid assessment of the diversity of microbial community

The use of macroarray as a simple tool to follow the metabolic profile of Lactobacillus plantarum during fermentation

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Valorization of non-wood biomass - case studies with food industry side-streams and municipal foodwaste

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Taxon abundance, diversity, co-occurrence and network analysis of the ruminal microbiota in response to dietary changes in dairy cows

We thank Mari Talvisilta and the staff in the metabolism unit at Natural Resources Institute Finland for technical support, care of experimental animals and assistance in sample collection. We thank Paula Lidauer for ruminal cannulation surgeries, Richard Hill from Aberystwyth University, UK for performing qPCR and Aurélie Bonin from Laboratoire d'Ecologie Alpine, CNRS, France for preparing archaea amplicon libraries for sequencing. Kevin J. Shingfield passed away before the submission of the final version of this manuscript. Ilma Tapio accepts responsibility for the integrity and validity of the data collected and analyzed. Funding: Study was funded by the Finnish Ministry of Agriculture and Forestry as part of the GreenDairy Project (Developing Genetic and Nutritional Tools to Mitigate the Environmental Impact of Milk Production; Project No. 2908234). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Carrot by-product fermentation quality and aerobic spoilage could be modified with silage additives

Vegetable by-products could potentially be suitable to be used as feeds in animal diets, which in general would strengthen the sustainability of both food and feed systems. Two experiments were performed with the objective to evaluate the aerobic spoilage via visual inspection and short term ensiling process of carrot by-products with silage additives (lactic acid bacteria [LAB] inoculants or formic acid [FA]). In the first experiment (Exp. 1), carrot by-product was treated with five additives including a control without additive, two commercial LAB inoculants (heterofermentative and homofermentative), an in-house isolated LAB mixture and a commercial FA based product. Second experiment (Exp. 2) used a 2 × 3 factorial design (type of raw material [carrot by-product with or without pieces] and additive treatment [control, heterofermentative LAB inoculant or FA based product]). Use of FA restricted lactic acid fermentation indicated by the high residual water soluble carbohydrate concentration while simultaneously stimulating ethanol production. LAB inoculants were not able to efficiently affect quality of ensiled carrot by-products. Use of FA also delayed the spoilage of the fresh carrot by-product particularly in Exp. 1

Suomalaisen ruishapanjuuren mikrobiston monimuotoisuus

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Leuconostoc Strains Unable to Split a Lactose Analogue Revealed by Characterisation of Mesophilic Dairy Starters

Mesophilic starter cultures used in dairy industry have been traditionally characterised by metabolic and biochemical methods. As closely related species of lactic acid bacteria have often only minor differences in phenotypic traits, which may also be variable within certain species, clear identification is often complicated. Therefore, techniques of molecular biology have been applied for rapid detection and differentiation of lactic acid bacteria. In this work, some bacterial clones isolated from mesophilic starters, which were preliminary identified as lactococci by phenotypic methods, were found to be Leuconostoc strains by both PCR and PFGE. According to the results, genotypic differentiation methods used in combination with phenotypic tests provide a fast and convenient way to reliably identify lactic acid bacteria displaying atypical metabolic characteristics

Effect of Potato By-Product on Production Responses of Dairy Cows and Total Mixed Ration Stability

Vegetable by-products have great potential for use as animal feeds and thus could improve the sustainability of the food system. The objective was to evaluate the milk production potential of potato by-product (PBP) replacing cereal grains in grass silage-based total mixed ration (TMR). Additionally, a laboratory scale experiment was conducted to assess the effect of PBP and chemical preservatives on the aerobic stability of TMRs. A change-over dairy cow feeding experiment was conducted to evaluate the inclusion of 135 g/kg (on a dry matter (DM) basis) of PBP in TMR. Additionally, the aerobic stability of TMR was evaluated by preparing TMRs with increasing levels of PBP and treated with different doses of formic and propionic acid or salt-based preservatives. The inclusion of PBP in dairy cow diets decreased feed intake, but organic matter digestibility was greater in the PBP diet, resulting in only slightly decreased production (milk protein 1435 vs. 1363 g/day, p < 0.05; milk lactose 1716 vs. 1606 g/day, p < 0.05). The aerobic stability of TMR was negatively affected by the inclusion of PBP, but it could to a limited extent be prolonged using chemical preservatives. The inclusion of PBP decreased feed intake and milk production to some extent when included in the diet of high-producing dairy cows. The utilization of PBP may, however, be justified from a sustainability point of view, as it provides a way to circulate the nutrients of a non-human edible feed material back into the food chain