Going To Pieces

Person playing the piano, with the whole illustration being in many separate pieceshttps://scholarsjunction.msstate.edu/cht-sheet-music/13741/thumbnail.jp

Bait sprays against the European cherry fruit fly Rhagoletis cerasi: Status Quo & Perspectives

Bait spray experiments against Rhagoletis cerasi were carried out in 2005 to 2007. The main principle of this method is to use food (e.g. sugar and proteins) as baits for the flies with small amounts of insecticides, which is applied on parts of the cherry tree. Main focus was aimed at laboratory and field cage experiments using the commercial GF-120 Naturalyte Fruit Fly BaitTM with the insecticide spinosad, which is registered against North American cherry fruit fly species in the US and Canada. Furthermore, additional bait spray formulations as alternatives to GF-120 were used to define the right food bait quality for a high attractiveness and low reproduction ability. Beyond, the negative effect on reproduction of flies for azadirachtin (neem) was shown. During this project, promising results, e.g. efficacies up to 99 % with infestation rates below the infestation threshold were obtained. From 2008 on, other insecticides, like azadirachtin (neem) and pyrethrine in an appropriate food bait mixture will be used. Questions like efficacy, adequate concentrations, persistence, rainfastness and practicability in commercial cherry orchards will be answered

Three-dimensional conformation at the H19/Igf2 locus supports a model of enhancer tracking

Insight into how the mammalian genome is structured in vivo is key to understanding transcriptional regulation. This is especially true in complex domains in which genes are coordinately regulated by long-range interactions between cis-regulatory elements. The regulation of the H19/Igf2 imprinted region depends on the presence of several cis-acting sequences, including a methylation-sensitive insulator between Igf2 and H19 and shared enhancers downstream of H19. Each parental allele has a distinct expression pattern. We used chromosome conformation capture to assay the native three-dimensional organization of the H19/Igf2 locus on each parental copy. Furthermore, we compared wild-type chromosomes to several mutations that affect the insulator. Our results show that promoters and enhancers reproducibly co-localize at transcriptionally active genes, i.e. the endodermal enhancers contact the maternal H19 and the paternal Igf2 genes. The active insulator blocks traffic of the enhancers along the chromosome, restricting them to the H19 promoter. Conversely, the methylated inactive insulator allows the enhancers to contact the upstream regions, including Igf2. Mutations that either remove or inhibit insulator activity allow unrestricted access of the enhancers to the whole region. A mutation that allows establishment of an enhancer-blocker on the normally inactive paternal copy diminishes the contact of the enhancer with the Igf2 gene. Based on our results, we propose that physical proximity of cis-acting DNA elements is vital for their activity in vivo. We suggest that enhancers track along the chromosome until they find a suitable promoter sequence to interact with and that insulator elements block further tracking of enhancers

Análise da Viabilidade Financeira Para Utilização de Estufas na Produção de Alface Hidropônica: Um Estudo de Caso Com o Uso da Metodologia Multi-Índices

The purpose of this article is to investigate the financial viability for producing hydroponic lettuce while using hydroponic systems (greenhouses) in the city of Mandirituba/PR. In order to carry out our analysis, we did some interviews on farms using greenhouses for agricultural production, and then we calculated the NPV, the IRR, the discounted payback and the profitability index of our study. The methodology we used was the multi-factor indexes one so that we could measure the risks associated with the project. The results showed a NPV of R$110.120,75 and an Internal Rate of Return of 51$ 110.120,75 e uma Taxa Interna de Retorno de 51%. Os indicadores de risco payback/N e GCR mostraram-se como médios. Já os indicadores risco de gestão, risco de negócios e TMA/TIR foram baixo/médios. Por último, o indicador ROIA, foi classificado como médio, uma vez que adiciona 4,23% de riqueza ao projeto, acima da TMA. Diante disto, confirmou-se a viabilidade financeira do projeto

A novel cross-sector telemedical approach to detect arrhythmia in primary care patients with palpitations using a patient-activated event recorder

Background: Patient-activated event recorders (ER) can facilitate diagnosis in unclear palpi­tations, however impact of ER screening on further treatment in clinical routine is unknown. We investigated the feasibility and clinical value of a network-based telemetric monitoring using a patient activated ER. Methods: The network consisted of 12 general practitioners (GP) and a department of car­diology (DC). GP-patients sent electrocardiograms (ECGs) twice daily and in case of palpitations. ECGs were transferred by email to GP and DC and analyzed independently by both. The therapeutic strategy was discussed between GP and DC. The monitoring period ended after 4 weeks or in case of detected arrhythmia. Results: A group of 184 consecutive patients were retrospectively analyzed. Mean age was 57.5 ± 14.4 years (range 17–82), 104 (56.5%) were female. Significant arrhythmia occurred in 71 (38.5%) patients: Recurrence of known paroxysmal atrial fibrillation (AF; n = 27, 14.7%), de novo AF (n = 19, 10.3%), premature complexes/bigeminus (n = 13, 7.1%), sinus tachycar­dia (n = 7, 3.8%), atrioventricular nodal reentrant tachycardia (n = 3, 1.6%), and ventricular tachycardia (n = 2, 1.1%). A therapeutic consequence resulted in 63 (88.7%) patients with de­tected arrhythmia: new oral anticoagulation (n = 29, 40.8%), new antiarrhythmic medication (n = 27, 38.0%), behavioral intervention (n = 19, 26.8%), electrophysiology-study/catheter ablation (n = 4, 5.6%), cardioversion (n = 2, 2.8%), implantable cardioverter-defibrillator- -implantation (n = 1, 1.4%), and left atrial appendage occluder (n = 1, 1.4%). Conclusions: The investigated cross-sector telemetric network is a feasible approach to detect arrhythmia in patients with palpitations and may have high impact on further treatment, notably in those at risk for stroke due to AF