Genetic diversity in tilapia populations in a freshwater reservoir assayed by randomly amplified polymorphic DNA markers

Genetic variation in fish stocks decreasing due to water pollution in the freshwater rivers, streams and canals. The objective of this study was to determine the genetic diversity and polymorphism in Oreochromis niloticus collected from the Wadi Hanefah Riyadh, Saudi Arabia by using RAPD-PCR. Total thirty fish specimens were harvested from each of four pre-determined locations of the reservoir which were designated as H1, H2, H3, and H4. Five random decamer primers were used to assess the diversity in the stock of O. niloticus. In this fish stock 48 bands were polymorphic and 12 were monomorphic. The maximum polymorphism (100%) was recorded in the fish samples procured from H4, followed by 88.75, 87.33 and 76.12% of the tilapia collected from H3, H2, and H4, respectively. Nei’s genetic distance value was ranged as 0.0005 to 0.1006. Maximum and minimum genetic distance was recorded as 0.1006 and 0.005 in tilapia harvested from H1 and H2 locations. Average heterozygosity was ranged from 0.3009 to 0.3744. This information about the genetic polymorphism of O. niloticus may be used by the concerned authorities to evolve strategies to conserve the diversity of tilapia in the country. Keywords: Genetic diversity, Tilapia, Primers, RAP

Effect of Heavy Metals on Liver, Kidney, Gills and Muscles of Cyprinus carpio and Wallago attu inhabited in the Indus

ABSTRACT: The present study was aimed to evaluate the effect of heavy metals on an important tissue of two fish species Cyprinus carpio and Wallago attu, sampled from the Indus river, Mianwali District, Pakistan. The concentration of selected heavy metals Fe, Cr, Cu, and in gills, muscles, kidney and liver was compared with an International standard of food fish. The overall metal concentrations among different weight categories in C. carpio were in the order of Fe > Cu > Cr >. In W. attu the overall accumulation of these metals were, in order of Fe > Cu > Cr > Pb The order of accumulation of metals in gills and muscle of C. carpio was Fe > Cr > Pb > Cu; kidney and muscles of W. attu was Fe > Cr > Cu > Pb; liver Fe > Cu > Cr > Pb. An increasing trend of concentration of iron, copper, chromium and lead occurred with an increase in weight of C. carpio and W. attu. There was a significant difference in the accumulation of heavy metals in different organs of both species (p<0.01). All studied heavy metals except Cr were within permissible limits described by various international agencies like WHO, FAO and FEPA in edible tissues of C. carpio and W. attu

Preface

The 1st International Scientific Conference on Pure Science (ISCPS2019) was held on 23 -24 January 2019 at the University of Al-Qadisiyah, College of Education, Diwaniyah, South of Iraq. The ISCPS2019 was the first forum to present the innovative results of the advanced topics in physics, mathematics, biology, and chemistry. The conference was jointly organised by the Ministry of Higher Education and Scientific Research in Iraq. The conference is an opportunity to achieve the strategic goals on the processes of activating the own capability to build (knowledge society), and keep abreast of scientific developments in the fields of science, and their applications and investments to serve the society. Thus, the conference aims is to bring together leading academics, scholars and students to discuss theoretical and practical issues to share their experiences and research findings, as well as ideas, problems and solutions related to the multifaceted aspects of Advanced Science. In this Conference, 300 technical papers were presented by local and international participants. The event included the contributions of large number of researchers, elite of universities and scientific institutions inside and outside Iraq. The contributions focused on the exchange of ideas and the dissemination of scientific knowledge. A number of reviewers assessed the quality of manuscripts. A rigorous peer-review process have implemented to evaluate the manuscripts. All accepted papers will be published in the conference proceeding. A booklet of abstracts was given to all participants on the registration day. The Committee have selected the high-quality manuscripts for publication in a special issue within a recognized international journal (with Clarivate and Scopus Indexing). The organised committee would like to thank the editors, Keynote speakers, reviewers and authors who have contributed to the Conference Proceedings. Our special thanks go to the Iraqi Higher Education and Scientific Research for their support towards the successful organization of the conference