Evaluation of Effectiveness of Bioactive Principles of Mucuna pruriens Seeds Using Experimental Models of Depression Associated with Parkinsonism and Associated Neurotransmitter Turnover

Background: Mucuna pruriens Linn. (DC) seeds are natural popular remedy, clinically used for the management of Parkinson’s disease. Depression is most common non-motor symptom associated with Parkinsonism. Present study evaluates effect of bioactive constituents of the M. pruriens seeds in experimental models of depression associated with Parkinsonism. Methods and Findings: Effect of 14 days treatment of isolated levodopa (ILD), alkaloid fraction (AF) and amino acid fraction (AAF) of the M. pruriens seeds were investigated in the catalepsy test, forced swim test, rotarod test and locomotor activity test after haloperidol challenge in mice. Further, the effects of Riboxetine, Bromocriptine and Fluoxetine were also studied in all these tests. The level of mice brain noradrenaline, dopamine and serotonin were assessed after 14 days treatment of all groups of mice. 14 days treatment of ILD (100 mg/kg, 200 mg/ kg, p.o.) AF (200 mg/kg, 400 mg/kg, p.o.) produced significant (P<0.001) reversal of haloperidol induced catalepsy, depression and motor performance along with rise in brain noradrenaline, dopamine and serotonin in mice. Reboxetine (1 mg/kg, i.p.) and Bromocriptine (2 mg/kg, i.p.) also showed similar effect except action on serotonin level. However, AAF (200 mg/kg, 400 mg/kg, p.o.) and did not significantly modify haloperidol induced catalepsy, depression and motor performance in mice. Fluoxetine (20 mg/kg, i.p.) also failed to reverse any effect of haloperidol in experimental models except it was potentiated haloperidol induced catalepsy in mice. AAF and Fluoxetine caused significant (P<0.001) reversal of haloperidol induced decrease in brain serotonin without modification of noradrenaline and dopamine level in mice. Conclusion: The behavioral and biochemical results of the present study indicate that ILD and AF of M. pruriens seeds have noradrenergic, dopaminergic and serotonergic system mediated protective action in experimental models of depression associated with Parkinsonism. Further clinical study requires to validating these findings. In addition, this study also provides evidence of protective action of Bromocriptine and Reboxetine. This study also confirms controversy action of an antidepressant fluoxetine in cataleptic depression

GIS as an educational tool: Mapping cultural sites in greek space-time

Το θέμα της παρούσας εργασίας είναι η χαρτογραφική παρουσίαση της πολιτιστικής διαδοχής στον ελληνικό χωρο-χρόνο που σχετίζεται με βασικές έννοιες της γεωγραφικής και ιστορικής εκπαίδευσης. Η παιδαγωγική αξία της μελέτης είναι η ανάπτυξη πέντε διακριτών δεξιότητων: της έννοιας του χρόνου-κλίμακας, της ιστορικής και γεωγραφικής κατανόησης, της χωρικής ανάλυσης και ερμηνείας, της ικανότητας διεξαγωγής γεω-ιστορικής έρευνας, και της γεω-ιστορικής διαδικασίας λήψης αποφάσεων. Η μεθοδολογία βασίζεται στη βαθμονόμηση μιας σειράς κριτηρίων για κάθε πολιτιστική περιοχή που καλύπτει τα θέματα της οικονομίας, της γεωμορφολογίας, της κοινωνίας των πολιτών, της θρησκείας, της τέχνης και της επιστήμης. Η περαιτέρω ανάλυση αυτών των δεδομένων οδηγεί στη δημιουργία μιας γεω-βάσης. Οι παλαιογεωγραφικοί και ιστορικοί χάρτες των πολιτιστικών χώρων που προέρχονται από την γεωβάση παρέχουν πληροφορίες σχετικά με τις χρονικές και χωρικές μεταβολές. Ως αποτέλεσμα, οι μαθητές θα είναι σε θέση να αναπτύξουν μια πολυδιάστατη και διεπιστημονική προσέγγιση, προκειμένου να ανακατασκευάσουν την εξέλιξη του τόπου.This paper deals with the cartographical presentation of cultural succession in Greek space-time associated with core concepts of geographic and historical education. The pedagogic value of this study is to develop five distinct skills: sense of time-scale, historical and geographic comprehension, spatial analysis and interpretation, ability to perform geo-historical research, and procedure of geohistorical decision-making. The methodology is based on the calibration of a set of criteria for each cultural site that covers the topics of economy, geomorphology, society, religion, art and science. Further analysis of these data forms a geodatabase. In addition, palaeogeographic and historical maps of the cultural sites derived by the geodatabase provide information about temporal and spatial changes. As result, students will be able to develop a multidimensional and interdisciplinary approach, in order to reconstruct the evolution of the site

Imaging findings of right colon diverticulitis that mimics colon cancer: a case report

We present a case report of a patient with imaging findings of right colon diverticulitis that mimics colon cancer. The computed tomography showed a segment of narrowing with shoulder formation at ascending colon suggesting cancer. The colonoscopy and the follow-up imaging clarify the diagnosis of diverticulitis. We assess the value of computed tomography findings of acute diverticulitis in excluding cancer

Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece

AbstractObjective To investigate the underlying resistance mechanisms in 10 Klebsiella pneumoniae isolates.Methods Ten K. pneumoniae strains according to distinct bacteriocin typing and REP-PCR, were examined for their plasmid content, their ability to transfer their resistance to aminoglycosides and third-generation cephalosporins, and their production of aminoglycoside-modifying enzymes and β-lactamases.Results Transfer of resistance to the above-mentioned antibiotics as well as to co-trimoxazole and tetracycline in Escherichia coli strain RC 85 at a frequency of 5–106 was achieved for all strains by conjugation. Similar strains harbor a self-transferable multiresistant plasmid (80 kb) with similar EcoRI and HindIII restriction patterns. This plasmid encodes an extended-spectrum β-lactamase which confers high-level resistance to third-generation cephalosporins and aztreonam. It produces SHV-5 β-lactamase, as demonstrated by isoelectric focusing and DNA sequencing. Aminoglycoside resistance was co-transferred, and AAC(6′)-I, mediating resistance to gentamicin, tobramycin, netilmicin and amikacin, and AAC(3)-I, mediating resistance to gentamicin and sisomycin, were encoded in all isolates and their transconjugants, while APH(3′)-I, mediating resistance to kanamycin and neomycin, was encoded in seven strains.Conclusions It appears that a multiresistant transferable plasmid encoding the SHV-5 β-lactamase, causing unusually high resistance to ceftazidime and aztreonam, and the combination AAC(6′)-I + AAC(3)-I of acetylating enzymes causing, also resistance to all clinically available aminoglycosides, is established in K. pneumoniae in Greece

Morphological study of the calcaneofibular ligament in cadavers

The aim of the present study was to investigate the anatomical and morphological characteristics and the maximum elongation of the calcaneofibular ligament (CFL) in cadavers. In a sample of 72 cadaveric lower limbs the mean values of length, width, thickness, and angle with the sagittal plane were recorded for the CFL. The mean ligament&#8217;s length was 31.8 mm, and the mean width and thickness were 4.4 mm and 1.5 mm respectively. The mean angle with the sagittal plane was 51.11°. In 72.2% of the lower limbs studied, the ligament presented one band, while 22.2% and 5.6% of them were two-banded and three-banded respectively. A common origin with the anterior talofibular ligament (TFL) was found in 24 of the feet (33%). There were also 4 cases in which the anterior TFL was absent. Finally, we measured the maximal elongation of the ligament during extreme inversion and simultaneous dorsal flexion and found it to be 2.88 mm on average. We noticed and statistically verified that women presented a greater elongation compared to men. A precise knowledge of the origin, insertion, direction, and morphology of CFL is critical for ligament injuries in ankle sprains and during ankle reconstruction. Ligament elasticity plays an important role in the range of ankle motion and ligament shearing. Male and female ankle joints differ in several anthropometric characteristics and thus the genre differences in ligament elongation are of great interest. (Folia Morphol 2011; 70, 3: 180&#8211;184

Molecular characterization of Indian potato (Solanum tuberosum L.) varieties for cold-induced sweetening using SSR markers

Cold-induced sweetening developed during storage of potatoes (Solanum tuberosum L.) at low temperature is a crucial factor influencing the processing quality of potato tubers and remains one of the principal concerns of potato processing industry. Developing CIS-resistant genotypes is the most effective method to cope with this stress. In this study, the genetic diversity of 11 Indian potato varieties with different reactions to CIS was assessed using 10 SSR primers. The primers detected a total of 42 alleles arranged in 44 different configurations, among which 37 alleles (88%) were polymorphic. The polymorphic information content (PIC) value of the SSR locus ranged from 0.473 to 0.787 thus indicating a high utility of these markers for study of genetic diversity in potato. A number of polymorphic fragments appeared to be specific to a given sugar-forming group. Primer Sti007 generated one fragment Sti007131bp present only in all the high sugar-forming varieties. The dendrogram derived from Dice’s similarity coefficients among the 11 varieties could partially but efficiently differentiate close parents and sugar-forming groups among the varieties. These findings demonstrate the effectiveness of SSR markers to assess the genetic variation among potato cultivars in order to develop molecular markers associated with CIS to improve potato breeding programs

Grain morphological characterization and protein content of sixty-eight local rice (Oryza sativa L) cultivars from Cameroon

Rice (Oryza sativa L.) cultivated in Cameroon is appreciated by consumers for its nutritive quality and good taste. Diversity of 68 local rice cultivars was investigated via grain morphology and protein content characterization. The size and shape of grains were determined and used with yield parameters to classify the cultivars and perform Principal Component Analysis (PCA). Total protein content and glutelin content of eight selected cultivars (CMRGNd, CMRGDn, CMRGTï, CMRTBa, CMRDWb, CMRDTc3, CMRDTx5 and CMRDTx6) were evaluated by Bradford assay and correlation analysis of all the parameters studied was performed. Long size grains (42) were predominant over extra-long (16), medium (9) and short (1) grains. Slender shaped grains (36) were distinguished as well as medium (28) and bold (4) grains. The 68 cultivars were grouped into four clusters independent of their origins. PCA revealed three principal components accounting for 74.4% of total variation. Highest total protein content was observed in CMRGNd (14.3%) and highest glutelin content in CMRGDn (10.1 mgEqvBSA/g DW). Pearson correlation of the different variables revealed no significant correlation between total protein and glutelin contents with the agro-morphological parameters evaluated in this study. This suggests that none of these parameters could be descriptor for protein content. Positive correlation between grain length and yield (r = 0.7) suggests grain length as yield descriptor

Association of chronic obstructive pulmonary disease with morbidity and mortality in patients with peripheral artery disease: insights from the EUCLID trial

Background: Patients with chronic obstructive pulmonary disease (COPD) are at increased risk of developing lower extremity peripheral artery disease (PAD) and suffering PAD-related morbidity and mortality. However, the effect and burden of COPD on patients with PAD is less well defined. This post hoc analysis from EUCLID aimed to analyze the risk of major adverse cardiovascular events (MACE) and major adverse limb events (MALE) in patients with PAD and concomitant COPD compared with those without COPD, and to describe the adverse events specific to patients with COPD. Methods: EUCLID randomized 13,885 patients with symptomatic PAD to monotherapy with either ticagrelor or clopidogrel for the prevention of MACE. In this analysis, MACE, MALE, mortality, and adverse events were compared between groups with and without COPD using unadjusted and adjusted Cox proportional hazards model. Results: Of the 13,883 patients with COPD status available at baseline, 11% (n=1538) had COPD. Patients with COPD had a higher risk of MACE (6.02 vs 4.29 events/100 patient-years; p< 0.001) due to a significantly higher risk of myocardial infarction (MI) (3.55 vs 1.85 events/100 patient-years; p< 0.001) when compared with patients without COPD. These risks persisted after adjustment (MACE: adjusted hazard ratio (aHR) 1.30, 95% confidence interval [CI] 1.11– 1.52; p< 0.001; MI: aHR 1.45, 95% CI 1.18– 1.77; p< 0.001). However, patients with COPD did not have an increased risk of MALE or major bleeding. Patients with COPD were more frequently hospitalized for dyspnea and pneumonia (2.66 vs 0.9 events/100 patient-years; aHR 2.77, 95% CI 2.12– 3.63; p< 0.001) and more frequently discontinued study drug prematurely (19.36 vs 12.54 events/100 patient-years; p< 0.001; aHR 1.34, 95% CI 1.22– 1.47; p< 0.001). Conclusion: In patients with comorbid PAD and COPD, the risks of MACE, respiratory-related adverse events, and premature study drug discontinuation were higher when compared with patients without COPD. Registration: ClinicalTrials.gov: NCT01732822

Enhancement of Amygdaloid Neuronal Dendritic Arborization by Fresh Leaf Juice of Centella asiatica (Linn) During Growth Spurt Period in Rats

Centella asiatica (CeA) is a creeping herb, growing in moist places in India and other Asian Countries. Ayurvedic system of medicine, an alternate system of medicine in India, uses leaves of CeA for memory enhancement. Here, we have investigated the role of CeA fresh leaf juice treatment during growth spurt period of rats on dendritic morphology of amygdaloid neurons, one of the regions concerned with learning and memory. The present study was conducted on neonatal rat pups. The rat pups (7-days-old) were fed with 2, 4 and 6 ml/kg body of fresh leaf juice of CeA for 2, 4 and 6 weeks. After the treatment period, the rats were killed, brains removed and amygdaloid neurons impregnated with Silver nitrate (Golgi staining). Amygdaloid neurons were traced using camera lucida and dendritic branching points (a measure of dendritic arborization) and intersections (a measure dendritic length) quantified. These data were compared with those of age-matched control rats. The results showed a significant increase in dendritic length (intersections) and dendritic branching points along the length of dendrites of the amygdaloid neurons of rats treated with 4 and 6 ml/kg body weight/day of CeA for longer periods of time (i.e. 4 and 6 weeks). We conclude that constituents/active principles present in CeA fresh leaf juice has neuronal dendritic growth stimulating property; hence it can be used for enhancing neuronal dendrites in stress and other neurodegenerative and memory disorders