Schlieren Visualization of Shaping Air During Operation of an Electrostatic Rotary Bell Sprayer: Impact of Shaping Air on Droplet Atomization and Transport

Electrostatic rotary bell sprayers (ERBSs) are widely used in the automotive industry. In ERBS, atomization is facilitated using centrifugal forces which disintegrate the paint film inside the cup into droplets at the cup edge. The droplets are then transported by the flow of a shaping air (SA) and electrostatic forces to a target surface; the characteristics of these droplets dramatically influence the quality of a painted surface and the painting transfer efficiency. In the current paper, a novel Schlieren-based visualization of the shaping air in the absence of paint droplets was performed during a qualitative investigation to delineate shaping air flow behavior and its interaction with droplets and droplet transport. An infrared thermographic flow visualization (IRFV) method and droplet size measurement were used to complement the Schlieren data for providing insight into shaping air-droplet interactions. The results demonstrated the impact of different operating conditions on the SA flow pattern, and the influence SA has on the secondary atomization and transport of droplets. Hence, these experimental methods combine with a useful tool for optimizing SA configurations that improve spray quality, droplet transport, and the efficiency of ERBS operations

Short-Term Effects of Biogas Digestates and Pig Slurry Application on Soil Microbial Activity

The effect of four biogas digestates (BD-A, BD-B, BD-C, and BD-D) and pig slurry (PS) on soil microbial functions was assessed at application rates corresponding to 0–1120 kg NH4+-N ha−1. At dose corresponding to 140 kg NH4+-N ha−1, 30.9–32.5% of the carbon applied in BD-A, BD-C, and PS was utilized during 12 days, while for BD-B and BD-D corresponding utilization was 19.0 and 16.9%, respectively. All BDs resulted in net nitrogen assimilation at low rates (17.5–140 kg NH4+-N ha−1) but net mineralization dominated at higher rates. PS resulted in net mineralization at all application rates. All residues inhibited potential ammonium oxidation (PAO), with EC50-values ranging between 45 and 302 kg NH4+-N ha−1. Low rates of BDs appeared to weakly stimulate potential denitrification activity (PDA), while higher rates resulted in logarithmic decrease. The EC50-values for PDA were between 238 and 347 kg NH4+-N ha−1. No inhibition of PDA was observed after amendment with PS. In conclusion, biogas digestates inhibited ammonia oxidation and denitrification, which could be an early warning of potential hazardous substances in the digestates. However, this effect can also be regarded as positive, since it may reduce nitrogen losses

100% Renewable Energy Grid for Rural Electrification of Remote Areas: A Case Study in Jordan

Many developing countries suffer from high energy-import dependency and inadequate electrification of rural areas, which aggravates the poverty problem. In this work, Al-Tafilah in Jordan was considered as a case study, where the technical, economic, and environmental benefits of a decentralized hybrid renewable energy system that can match 100% of the city demand were investigated. A tri-hybrid system of wind, solar, and hydropower was integrated with an energy storage system and optimized to maximize the match between the energy demand and production profiles. The optimization aimed at maximizing the renewable energy system (RES) fraction while keeping the levelized cost of electricity (LCOE) equal to the electricity purchase tariff. The techno-economic analysis showed that the optimal system in Al-Tafilah comprises a 28 MW wind system, 75.4 MW PV, and 1 MW hydropower, with a 259 MWh energy storage system, for which a RES fraction of 99% can be achieved, and 47,160 MtCO2 are avoided. This study can be easily extended to other rural cities in Jordan, as they have higher renewable energy system (RES) potential. The presented findings are essential not only for Jordan’s planning and economy-boosting but also for contributing to the ongoing force against climate change

Microstructure, Elastic and Inelastic Properties of Partially Graphitized Biomorphic Carbons

The microstructural characteristics and amplitude dependences of the Young’s modulus E and internal friction (logarithmic decrement δ) of biocarbon matrices prepared by beech wood carbonization at temperatures Tcarb = 850–1600°C in the presence of a nickelcontaining catalyst have been studied. Using Xray diffraction and electron microscopy, it has been shown that the use of a nickel catalyst during carbon ization results in a partial graphitization of biocarbons at Tcarb ≥ 1000°C: the graphite phase is formed as 50 to 100nm globules at Tcarb = 1000°C and as 0.5 to 3.0μm globules at Tcarb = 1600°C. It has been found that the measured dependences E(Tcarb) and δ(Tcarb) contain three characteristic ranges of variations in the Young’s modulus and logarithmic decrement with a change in the carbonization temperature: E increases and δ decreases in the ranges Tcarb 1300°C; in the range 1000 < Tcarb < 1300°C, E sharply decreases and δ increases. The observed behavior of E(Tcarb) and δ(Tcarb) for biocarbons carbonized in the presence of nickel correlates with the evolution of their microstructure. The largest values of E are obtained for samples with Tcarb = 1000 and 1600°C. However, the samples with Tcarb = 1600°C exhibit a higher suscep tibility to microplasticity due to the presence of a globular graphite phase that is significantly larger in size and total volume.Peer reviewe

Bio-inspired artemether-loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes

Aim: The intra-erythrocytic development of the malarial parasite is dependent on active uptake of nutrients, including human serum albumin (HSA), into parasitized red blood cells (pRBCs). We have designed HSA-based nanoparticles as a potential drug-delivery option for antimalarials. Methods: Artemether-loaded nanoparticles (AANs) were designed and antimalarial activity evaluated in vitro/in vivo using Plasmodium falciparum/Plasmodium berghei species, respectively. Results: Selective internalization of AAN into Plasmodium-infected RBCs in preference to healthy erythrocytes was observed using confocal imaging. In vitro studies showed 50% dose reduction for AAN as compared with drug-only controls to achieve IC50 levels of inhibition. The nanoparticles exhibited twofold higher peak drug concentrations in RBCs with antimalarial activity at 50% of therapeutic doses in P. bergei infected mice. Conclusion: Novel HSA-based nanoparticles offer safe and effective approach for selective targeting of antimalarial drugs

Spatio-Temporal Pattern in the Changes in Availability and Sustainability of Water Resources in Afghanistan

Water is gradually becoming scarce in Afghanistan like in many other regions of the globe. The objective of this study was to evaluate the spatial changes in the availability and sustainability of water resources in Afghanistan. The Terrestrial Water Storage (TWS) data of the Gravity Recovery and Climate Experiment (GRACE) satellite obtained from three different institutes, having 1° × 1° spatial resolution for the period 2002–2016 was used for this purpose. Sen’s slope method was used to assess the rate of change, and the Modified Mann–Kendall test was used for the evaluation of the significance of trends in TWS. After, the concept of reliability–resiliency–vulnerability (RRV) was used for assessing the spatial distribution of sustainability in water resources. The results revealed a significant decrease in water availability in the country over the last 15 years. The decrease was found to be highest in the central region where most of the population of the country resides. The reliability in water resources was found high in the northeast Himalayan region and low in the southwest desert; resilience was found low in the central region, while the vulnerability was found high in the south and the southeast. Overall, the water resources of the country were found most sustainable in the northeast and southwest and least in the south and the central parts. The maps of water resource sustainability and the changes in water availability produced in the present study can be used for long-term planning of water resources for adaptation to global changes. Besides, those can be used for the management of water resources in a sustainable and judicious manner

A narrative inquiry into the identity formation of Chinese doctoral students in relation to study abroad

The present study explores a topic which has been under-studied to date, namely the identity formation of Chinese PhD students in relation to study abroad. Underpinned by Giddens' (1991) "reflexive project of the self", which privileges agency and reflexivity, and using a narrative inquiry approach, it presents four students' 'stories' collected through semi-structured interviews and focus group discussions in the form of a trajectory of development fro past to future. In building a picture of theways in which students' self-identity is shaped by and shapes their experience of study abroad, the stories illustrate individual agency, motivation, self-determination and reflexivity. In doing so, they challenge the essentialised view of Chinese students as a homogeneous and sometimes problematic group and point to implications for action by the host institutions

Inhibition of the JAK2/STAT3 pathway in ovarian cancer results in the loss of cancer stem cell-like characteristics and a reduced tumor burden

Background Current treatment of ovarian cancer patients with chemotherapy leaves behind a residual tumor which results in recurrent ovarian cancer within a short time frame. We have previously demonstrated that a single short-term treatment of ovarian cancer cells with chemotherapy in vitro resulted in a cancer stem cell (CSC)-like enriched residual population which generated significantly greater tumor burden compared to the tumor burden generated by control untreated cells. In this report we looked at the mechanisms of the enrichment of CSC-like residual cells in response to paclitaxel treatment. Methods The mechanism of survival of paclitaxel-treated residual cells at a growth inhibitory concentration of 50% (GI50) was determined on isolated tumor cells from the ascites of recurrent ovarian cancer patients and HEY ovarian cancer cell line by in vitro assays and in a mouse xenograft model. Results Treatment of isolated tumor cells from the ascites of ovarian cancer patients and HEY ovarian cancer cell line with paclitaxel resulted in a CSC-like residual population which coincided with the activation of Janus activated kinase 2 (JAK2) and signal transducer and activation of transcription 3 (STAT3) pathway in paclitaxel surviving cells. Both paclitaxel-induced JAK2/STAT3 activation and CSC-like characteristics were inhibited by a low dose JAK2-specific small molecule inhibitor CYT387 (1 μM) in vitro. Subsequent, in vivo transplantation of paclitaxel and CYT387-treated HEY cells in mice resulted in a significantly reduced tumor burden compared to that seen with paclitaxel only-treated transplanted cells. In vitro analysis of tumor xenografts at protein and mRNA levels demonstrated a loss of CSC-like markers and CA125 expression in paclitaxel and CYT387-treated cell-derived xenografts, compared to paclitaxel only-treated cell-derived xenografts. These results were consistent with significantly reduced activation of JAK2 and STAT3 in paclitaxel and CYT387-treated cell-derived xenografts compared to paclitaxel only-treated cell derived xenografts. Conclusions This proof of principle study demonstrates that inhibition of the JAK2/STAT3 pathway by the addition of CYT387 suppresses the ‘stemness’ profile in chemotherapy-treated residual cells in vitro, which is replicated in vivo, leading to a reduced tumor burden. These findings have important implications for ovarian cancer patients who are treated with taxane and/or platinum-based therapies. Keywords: Ovarian carcinoma, Cancer stem cell, Metastasis, Ascites, Chemoresistance, Recurrence, JAK2/STAT3 pathwa

Investigation into the controversial association of Streptococcus gallolyticus with colorectal cancer and adenoma

Background: The seroprevalence of IgG antibodies of Streptococcus gallolyticus subspecies gallolyticus, CIP 105428, was evaluated to investigate the controversial association of S. gallolyticus with colorectal carcinoma and adenoma in attempt to investigate the nature of such association if any, by exploring the mRNA expression of NF-κB and IL-8. Moreover, the serological behavior of S. gallolyticus IgG antibodies was compared to that of an indicator bacterium of bowel, Bacteroides fragilis. Methods: ELISA was used to measure IgG antibodies of S. gallolyticus and B. fragilis in sera of 50 colorectal cancer, 14 colorectal adenoma patients, 30 age- and sex- matched apparently healthy volunteers (HV) and 30 age- and sex- matched colonoscopically-proven tumor-free control subjects. NF-κB and IL-8 mRNA expression was evaluated in tumorous and non-tumorous tissue sections of carcinoma and adenoma patients in comparison with that of control subjects by using in situ hybridization assay. Results: Colorectal cancer and adenoma patients were associated with higher levels of serum S. Gallolyticus IgG antibodies in comparison with HV and control subjects (P 0.05). ELISA cutoff value for the seropositivity of S. gallolyticus IgG was calculated from tumor-free control group. The expression of NF-κB mRNA was higher in tumorous than non-tumorous tissue sections of adenoma and carcinoma, higher in carcinoma/adenoma sections than in control subjects, higher in tumorous sections of carcinoma than in adenoma patients, and higher in S. gallolyticus IgG seropositive than in seronegative groups in both tumorous and non-tumorous sections (P < 0.05). IL-8 mRNA expression in tumorous sections of adenoma and carcinoma was higher than in non-tumorous sections, higher in carcinoma/adenoma than in control subjects, and higher in S. gallolyticus IgG seropositive than in seronegative groups in tumorous rather than non-tumorous sections (P < 0.05). Conclusion: S. gallolyticus most likely plays an essential role in the oncogenic progression of normal colorectal mucosa to adenoma and to CRC. This promoting/propagating role of S. gallolyticus might take place by utilizing certain inflammatory, anti-apoptotic, and angiogenic factors of transformation including NF-κB and IL-8.Ahmed S Abdulamir, Rand R Hafidh, Layla K Mahdi, Tarik Al-jeboori and Fatimah Abubake