Agrobacterium-mediated transformation of Mycosphaerella fijiensis, the devastating Black Sigatoka pathogen of bananas

Mycosphaerella fijiensis, M. musicola en M. eumusae veroorzaken de Sigatoka-ziekte in banaan. Op dit moment is de toepassing van fungiciden de enige optie om deze ziekte te bestrijden. Het PRPB (Pesticide Reduction Program for Banana) investeert in de ontwikkeling van technieken voor de genotype- en fenotypebepaling van M. fijiensis. Hierbij wordt gebruikt gemaakt van ATMT (Agrobacterium tumefaciens-mediated transformation)

The Height of Chromospheric Loops in an Emerging Flux Region

Context. The chromospheric layer observable with the He I 10830 {\AA} triplet is strongly warped. The analysis of the magnetic morphology of this layer therefore requires a reliable technique to determine the height at which the He I absorption takes place. Aims. The He I absorption signature connecting two pores of opposite polarity in an emerging flux region is investigated. This signature is suggestive of a loop system connecting the two pores. We aim to show that limits can be set on the height of this chromospheric loop system. Methods. The increasing anisotropy in the illumination of a thin, magnetic structure intensifies the linear polarization signal observed in the He I triplet with height. This signal is altered by the Hanle effect. We apply an inversion technique incorporating the joint action of the Hanle and Zeeman effects, with the absorption layer height being one of the free parameters. Results. The observed linear polarization signal can be explained only if the loop apex is higher than \approx5 Mm. Best agreement with the observations is achieved for a height of 6.3 Mm. Conclusions. The strength of the linear polarization signal in the loop apex is inconsistent with the assumption of a He I absorption layer at a constant height level. The determined height supports the earlier conclusion that dark He 10830 {\AA} filaments in emerging flux regions trace emerging loops.Comment: 7 pages, 4 figure

Observations of solar scattering polarization at high spatial resolution

The weak, turbulent magnetic fields that supposedly permeate most of the solar photosphere are difficult to observe, because the Zeeman effect is virtually blind to them. The Hanle effect, acting on the scattering polarization in suitable lines, can in principle be used as a diagnostic for these fields. However, the prediction that the majority of the weak, turbulent field resides in intergranular lanes also poses significant challenges to scattering polarization observations because high spatial resolution is usually difficult to attain. We aim to measure the difference in scattering polarization between granules and intergranules. We present the respective center-to-limb variations, which may serve as input for future models. We perform full Stokes filter polarimetry at different solar limb positions with the CN band filter of the Hinode-SOT Broadband Filter Imager, which represents the first scattering polarization observations with sufficient spatial resolution to discern the granulation. Hinode-SOT offers unprecedented spatial resolution in combination with high polarimetric sensitivity. The CN band is known to have a significant scattering polarization signal, and is sensitive to the Hanle effect. We extend the instrumental polarization calibration routine to the observing wavelength, and correct for various systematic effects. The scattering polarization for granules (i.e., regions brighter than the median intensity of non-magnetic pixels) is significantly larger than for intergranules. We derive that the intergranules (i.e., the remaining non-magnetic pixels) exhibit (9.8 \pm 3.0)% less scattering polarization for 0.2<u<0.3, although systematic effects cannot be completely excluded. These observations constrain MHD models in combination with (polarized) radiative transfer in terms of CN band line formation, radiation anisotropy, and magnetic fields.Comment: Accepted for publication in A&

A Field Guide for Grasses and Grass-like Plants of Idaho

The purpose of this project is to develop a user-friendly field guide to grasses and grass-like plants in Idaho, specifically geared to those with limited background in botany. The guide will feature 60 Idaho grasses and grass-like plants, intended for K-16 educators and students, ranchers, land owners, recreationists, and nature enthusiasts, with accompanying K-12 lesson plans. In the form of both a printed book and an offline app for iPhones and Androids, the guide will include colorful images showing detailed characteristics and vegetative features of each grass, an easy-to-use dichotomous key, and information on each plant’s history, forage value, and fire resistance. This dual resource will meet the needs of land managers making economic decisions regarding livestock production and field treatments; university students in wildlife and range sciences conducting class exercises and field research; K-12 educators during field botany excursions, teaching the use of dichotomous keys, and ecosystem studies; and recreationists engaged in nature study. Both book and app will be distributed via the University of Idaho Rangeland Center and the Idaho Range Resource Commission

Direct measurement of the size of 2003 UB313 from the Hubble Space Telescope

We have used the Hubble Space Telescope to directly measure the angular size of the large Kuiper belt object 2003 UB313. By carefully calibrating the point spread function of a nearby field star, we measure the size of 2003 UB313 to be 34.3$\pm$1.4 milliarcseconds, corresponding to a diameter of 2400$\pm$100 km or a size $\sim5$% larger than Pluto. The V band geometric albedo of 2003 UB313 is $86\pm7$%. The extremely high albedo is consistent with the frosty methane spectrum, the lack of red coloring, and the lack of observed photometric variation on the surface of 2003 UB313. Methane photolysis should quickly darken the surface of 2003 UB313, but continuous evaporation and redeposition of surface ices appears capable of maintaining the extreme alebdo of this body

Origin of spatial variations of scattering polarization in the wings of the Ca {\sc i} 4227 \AA line

Polarization that is produced by coherent scattering can be modified by magnetic fields via the Hanle effect. According to standard theory the Hanle effect should only be operating in the Doppler core of spectral lines but not in the wings. In contrast, our observations of the scattering polarization in the Ca {\sc i} 4227 \AA line reveals the existence of spatial variations of the scattering polarization throughout the far line wings. This raises the question whether the observed spatial variations in wing polarization have a magnetic or non-magnetic origin. A magnetic origin may be possible if elastic collisions are able to cause sufficient frequency redistribution to make the Hanle effect effective in the wings without causing excessive collisional depolarization, as suggested by recent theories for partial frequency redistribution with coherent scattering in magnetic fields. To model the wing polarization we apply an extended version of the technique based on the "last scattering approximation". This model is highly successful in reproducing the observed Stokes $Q/I$ polarization (linear polarization parallel to the nearest solar limb), including the location of the wing polarization maxima and the minima around the Doppler core, but it fails to reproduce the observed spatial variations of the wing polarization in terms of magnetic field effects with frequency redistribution. This null result points in the direction of a non-magnetic origin in terms of local inhomogeneities (varying collisional depolarization, radiation-field anisotropies, and deviations from a plane-parallel atmospheric stratification).Comment: Accepted in May 2009 for publication in The Astrophysical Journa

Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.

The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate