33 research outputs found
A comparative analysis of meat inspection data as an information source of the health and welfare of broiler chickens based on Finnish data
The comprehensive, reliable, and comparable meat inspection (MI) data of broiler chickens (i.e. broilers) are essential for the monitoring and surveillance of broiler health and welfare at the national and European Union (EU) levels. We compared the condemnation causes issued to broiler carcasses during MI in four large Finnish broiler slaughterhouses (SHs) by investigating the similarities and differences between local MI instructions used in the SHs. The way in which MI condemnations were recorded in the Finnish Food Authority's (FFA's) MI statistics were also explored. We additionally analysed the FFA's official MI data from the 2015-2019 period. The study showed that the MI criteria used in the SHs differed from one another regarding how severe or extensive a broiler defect or disease must be to cause condemnation during MI. In Finland, the annual total condemnation prevalence of whole broilers varied between 2.6% and 4.8% in 2015-2019, and a significant difference was observed between the SHs' monthly total condemnation prevalences, except in two SH pairs. Mistakes in recording the FFA's MI statistics and differences in the SH operators' reasons to reject broilers from the food chain affect the comparability of the condemnation prevalences between the SHs. Only half of the SHs partially condemned broiler carcasses and collected data concerning these condemnations. Cellulitis (0.3-1.0%), ascites (0.3-0.4%), and body cavity disorders (0.2-0.3%) were the most common causes for condemning whole broiler carcasses in 2015-2019. The MI data can be used for monitoring and surveillance purposes only once the differences between the SH data and data reliability are known. Although the harmonization of all condemnation causes is impossible, harmonizing the condemnations of carcasses with diseases that most threaten broiler health and welfare and cause the largest economic losses would be important.Peer reviewe
Wilson ratio of a Tomonaga-Luttinger liquid in a spin-1/2 Heisenberg ladder
Using micromechanical force magnetometry, we have measured the magnetization
of the strong-leg spin-1/2 ladder compound (CHN)CuBr at
temperatures down to 45 mK. Low-temperature magnetic susceptibility as a
function of field exhibits a maximum near the critical field H_c at which the
magnon gap vanishes, as expected for a gapped one-dimensional antiferromagnet.
Above H_c a clear minimum appears in the magnetization as a function of
temperature as predicted by theory. In this field region, the susceptibility in
conjunction with our specific heat data yields the Wilson ratio R_W. The result
supports the relation R_W=4K, where K is the Tomonaga-Luttinger-liquid
parameter
Prerequisites of inspection conditions for uniform post-mortem inspection in broiler chicken slaughterhouses in Finland
Meat inspection of broiler chickens (broiler) in the European Union is regulated by common legislation to secure meat safety. However, the legislation is general in nature and proper post-mortem inspection (PMI) of every carcass and visceral organs of broilers is challenging in slaughterhouses (SHs) with a high slaughter line speed. The aim of this study was to investigate the on-site organization and possible differences of the PMI in four Finnish SHs, which slaughter over 99% of broilers in Finland. Our results show that the meat inspector's available inspection time per broiler in the PMI varied between 0.28 and 0.90 s, with the shortest available inspection time in the SH with the highest slaughter line speed and the longest available inspection time in the SH with the slowest line speed. We observed that only part of the total inspection time per broiler could be used for true PMI in most (3/4) SHs, as the meat inspectors also performed other tasks during the PMI. We observed deficiencies in the visual inspection of broiler carcasses; in particular, the proper inspection of all or most of the body cavities was impossible in all SHs during the PMI. Some deficiencies in facilities (e.g. in recording system) were observed. Moreover, lighting properties varied between the SHs and a significant difference between illumination conditions at the first inspection stations in the SHs was observed. This study considered the prerequisites for proper PMI and revealed that the PMI of broilers was not completely uniform in Finland. The results emphasize the need for more precise guidelines and recommendations, especially for inspection time and lighting at inspection stations.Peer reviewe
{Rearrangement of the antiferromagnetic ordering at high magnetic fields in SmFeAsO and SmFeAsOF single crystals
The low-temperature antiferromagnetic state of the Sm-ions in both
nonsuperconducting SmFeAsO and superconducting SmFeAsOF single
crystals was studied by magnetic torque, magnetization, and magnetoresistance
measurements in magnetic fields up to 60~T and temperatures down to 0.6~K. We
uncover in both compounds a distinct rearrangement of the antiferromagnetically
ordered Sm-moments near ~T. This is seen in both, static and pulsed
magnetic fields, as a sharp change in the sign of the magnetic torque, which is
sensitive to the magnetic anisotropy and hence to the magnetic moment in the
-plane, ({\it i.e.} the FeAs-layers), and as a jump in the magnetization
for magnetic fields perpendicular to the conducting planes. This rearrangement
of magnetic ordering in ~T is essentially temperature independent and
points towards a canted or a partially polarized magnetic state in high
magnetic fields. However, the observed value for the saturation moment above
this rearrangement, suggests that the complete suppression of the
antiferromagnetism related to the Sm-moments would require fields in excess of
60~T. Such a large field value is particularly remarkable when compared to the
relatively small N\'{e}el temperature ~K, suggesting very
anisotropic magnetic exchange couplings. At the transition, magnetoresistivity
measurements show a crossover from positive to negative field-dependence,
indicating that the charge carriers in the FeAs planes are sensitive to the
magnetic configuration of the rare-earth elements. This is indicates a finite
magnetic/electronic coupling between the SmO and the FeAs layers which are
likely to mediate the exchange interactions leading to the long range
antiferromagnetic order of the Sm ions.Comment: 10 pages, 7 figures, accepted in Phys. Rev.
Bose-Einstein Condensation of Triplons in Ba3Cr2O8
By performing heat capacity, magnetocaloric effect, torque magnetometry and
force magnetometry measurements up to 33 T, we have mapped out the T-H phase
diagram of the S = 1/2 spin dimer compound Ba3Cr2O8. We found evidence for
field-induced magnetic order between Hc1 = 12.52(2) T and Hc2 = 23.65(5) T,
with the maximum transition temperature Tc = 2.7 K at H = 18 T. The lower
transition can likely be described by Bose-Einstein condensation of triplons
theory, and this is consistent with the absence of any magnetization plateaus
in our magnetic torque and force measurements. In contrast, the nature of the
upper phase transition appears to be quite different as our measurements
suggest that this transition is actually first order.Comment: 4 pages, 4 figures, Accepted by PR
Floppy mitral valve/mitral valve prolapse syndrome: Beta-adrenergic receptor polymorphism may contribute to the pathogenesis of symptoms
AbstractBackgroundCertain patients with floppy mitral valve (FMV)/mitral valve prolapse (MVP) may have symptoms that cannot be explained on the severity of mitral valvular regurgitation (MVR) alone; hypersensitivity to adrenergic stimulation has been suggested in this group defined as the FMV/MVP syndrome.MethodsNinety-eight patients (75 men, 23 women) with mitral valve surgery for FMV/MVP were studied. Of those 41 (42%) had symptoms consistent with FMV/MVP syndrome [29 men (39%), 12 women (52%)]; median age of symptom onset was 30 years (range 10–63 years) and median duration of symptoms prior to valve surgery was 16 years (range 3–50 years). Ninety-nine individuals (70 men, 29 women) without clinical evidence of any disease were used as controls. Genotyping of β1 and β2 adrenergic receptors was performed.Resultsβ-Adrenergic receptor genotypes (β1 and β2) were similar between control and overall FMV/MVP patients. Subgroup analysis of patients, however, demonstrated that the genotype C/C at position 1165 resulting in 389 Arg/Arg of the β1 receptor was more frequent in women compared to those without FMV/MVP syndrome and to normal control women (p<0.025). This polymorphism may be related to hypersensitivity to adrenergic stimulation as reported previously in these patients.ConclusionThis study shows a large proportion of patients with FMV/MVP, predominantly women, had symptoms consistent with the FMV/MVP syndrome for many years prior to the development of significant MVR, and thus symptoms cannot be attributed to the severity of MVR alone. Further, women with FMV/MVP syndrome, symptoms at least partially may be related to β1-adrenergic receptor polymorphism, which has been shown previously to be associated with a hyperresponse to adrenergic stimulation
Histone Deacetylase Inhibitors Globally Enhance H3/H4 Tail Acetylation Without Affecting H3 Lysine 56 Acetylation
Histone deacetylase inhibitors (HDACi) represent a promising avenue for cancer therapy. We applied mass spectrometry (MS) to determine the impact of clinically relevant HDACi on global levels of histone acetylation. Intact histone profiling revealed that the HDACi SAHA and MS-275 globally increased histone H3 and H4 acetylation in both normal diploid fibroblasts and transformed human cells. Histone H3 lysine 56 acetylation (H3K56ac) recently elicited much interest and controversy due to its potential as a diagnostic and prognostic marker for a broad diversity of cancers. Using quantitative MS, we demonstrate that H3K56ac is much less abundant than previously reported in human cells. Unexpectedly, in contrast to H3/H4 N-terminal tail acetylation, H3K56ac did not increase in response to inhibitors of each class of HDACs. In addition, we demonstrate that antibodies raised against H3K56ac peptides cross-react against H3 N-terminal tail acetylation sites that carry sequence similarity to residues flanking H3K56
Multilocus Sequence Typing as a Replacement for Serotyping in Salmonella enterica
Salmonella enterica subspecies enterica is traditionally subdivided into serovars by serological and nutritional characteristics. We used Multilocus Sequence Typing (MLST) to assign 4,257 isolates from 554 serovars to 1092 sequence types (STs). The majority of the isolates and many STs were grouped into 138 genetically closely related clusters called eBurstGroups (eBGs). Many eBGs correspond to a serovar, for example most Typhimurium are in eBG1 and most Enteritidis are in eBG4, but many eBGs contained more than one serovar. Furthermore, most serovars were polyphyletic and are distributed across multiple unrelated eBGs. Thus, serovar designations confounded genetically unrelated isolates and failed to recognize natural evolutionary groupings. An inability of serotyping to correctly group isolates was most apparent for Paratyphi B and its variant Java. Most Paratyphi B were included within a sub-cluster of STs belonging to eBG5, which also encompasses a separate sub-cluster of Java STs. However, diphasic Java variants were also found in two other eBGs and monophasic Java variants were in four other eBGs or STs, one of which is in subspecies salamae and a second of which includes isolates assigned to Enteritidis, Dublin and monophasic Paratyphi B. Similarly, Choleraesuis was found in eBG6 and is closely related to Paratyphi C, which is in eBG20. However, Choleraesuis var. Decatur consists of isolates from seven other, unrelated eBGs or STs. The serological assignment of these Decatur isolates to Choleraesuis likely reflects lateral gene transfer of flagellar genes between unrelated bacteria plus purifying selection. By confounding multiple evolutionary groups, serotyping can be misleading about the disease potential of S. enterica. Unlike serotyping, MLST recognizes evolutionary groupings and we recommend that Salmonella classification by serotyping should be replaced by MLST or its equivalents
Sheep carrying pathogenic Yersinia enterocolitica bioserotypes 2/O:9 and 5/O:3 in the feces at slaughter
belonging to biotypes 1B and 2-5. Pathogenic strains of biotypes 2-4 carrying the ail virulence gene have frequently been isolated from domestic pigs at slaughter. In sheep, mostly non-pathogenic biotype 1A strains have been reported. In our study, the prevalence of ail-positive Y. enterocolitica was studied by PCR and culturing in 406 young sheep (<1year of age) and 139 older sheep at slaughter in Finland. When using PCR, the detection rate was 11% (45/406) in young sheep originating from 11 (18%) farms. Surprisingly, Y. enterocolitica belonging to bioserotypes 2/O:9 and 5/O:3, carrying both chromosomal and plasmid-borne virulence genes, were isolated from the fecal samples of 10 (2%) and 23 (4%) sheep, respectively. All isolates of bioserotypes 2/O:9 (19 isolates) and 5/O:3 (53 isolates) carried the chromosomal virulence genes ail, inv, ystA, and myfA, and almost all isolates (71/72) also carried the virulence genes virF and yadA located on the virulence plasmid. The isolates showed high susceptibility to tested antimicrobials and low genetic diversity by PFGE. Y. enterocolitica bioserotype 5/O:3 is a very rare bioserotype, and has earlier only sporadically been reported in European wildlife and in sheep in Australia and New Zealand. Bioserotype 2/O:9 is a common bioserotype found in humans with yersiniosis, and has sporadically been isolated in wild and domestic animals