Identification of scorpion species (arachnids: scorpions) collected from selected areas in the Jaffna Peninsula

Taxonomic record on scorpion (Arthropoda: Arachnids: Scorpions) is scarce in Sri Lanka. Several families of scorpions, namely Scorpionidae, Charitable and Buthidae were recorded in Yale national park, Kandy, Trincomalee and Mullaittivu in Sri Lanka, based on the collection of the Natural History Museum of the city of Geneva. It is reported that dangerous scorpions are not found in Sri Lanka and Malaysia. Recently identified scorpions of Family Buthidae in some parts of India. But to date no one has reported on scorpion species of Families Buthidae & Scorpionidae in the Jaffna peninsula. Field collection was carried out in selected areas including Vadamarachi, Atchuvelly, Thirunelveli, Kopay and Kondavil in the Nonhern Province from September 2010 to March 2011. Collected scorpions were identified with the help of descriptions and illustrations provided by Pocock (1900) and Bucherl (1971). Identification was performed with the aid of dissecting microscope (Kyowa, Photographs of life specimens were taken with a digital camera (Sony). Lined diagrams were drawn with the help of camera lucida stereo microscope (Olympus BX 51). Descriptive terms of Pocock were mostly followed. Two families, namely, Buthidae and Scorpionidae (Koch, 1837) were identified based on colouration, shape of the sternum, tarsal spur and pedal spur present on the leg. Further identification revealed the presence of two species, namely, Bu thus Dorian and Palamnaeus wrongdoing based on pectin teeth and keels on the caudal segment. The present preliminary survey should be continued in order to identify any other scorpion species in other parts of the Jaffna peninsula

Exposure rate to zika virus infection in a sample of clinically diagnosed dengue patients presenting to the Teaching Hospital, Jaffna, Sri Lanka

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SYNTHESIS AND EVALUATION OF PHTHALATE ANALOGUE OF DICLOFENAC AGAINST FREUND’S COMPLETE ADJUVANT INDUCED ARTHRITIS IN RAT

Objective: The objective of the present study is to evaluate the effect of Phthalate analogues of diclofenac in Freund’s complete adjuvant (FCA) induced Arthritis in the rat. Methods: Twenty four female albino wistar rats were enrolled in this study and are divided into 4 groups (six each). The groups were designed as follows: Group I: vehicle control, Group II: arthritic control, Group III: diclofenac treated, Group IV: phthalate analogue of diclofenac treated. Various assessments such as anti-arthritic activity, biochemical estimations, haematological parameters, ulcerogenesis, radiological and histopathological studies were evaluated. Results: Arthritic control group exhibited significant increase in the level of paw volume, arthritic score (p<0.0001), Serum glutamic pyruvic transaminase (SGPT) (p<0.001), Serum glutamic oxaloacetic transaminase (SGOT) p<0.01), rheumatoid arthritis factor, C-reactive protein (CRP), White Blood Cells (WBC), Creatinine and uric acid and a significant decrease in Red Blood Cells (RBC). Increased swelling of joints, bony destruction and profound ulceration were observed in the Arthritic control group. All these conditions were reversed in diclofenac and phthalate analogue of diclofenac groups. Conclusion: We conclude that phthalate analogue of diclofenac shows potent anti-arthritic activity with milder ulceration when compared to diclofenac treatment

Seroprevalence of hepatitis A and E virus infections among Allied Health Sciences students of the Faculty of Medicine, University of Jaffna

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A mechanistic role for leptin in human dendritic cell migration: differences between ileum and colon in health and Crohn's disease

Dendritic cells (DC) migrate to lymph nodes on expression of C-C motif chemokine receptor 7 (CCR7) and control immune activity. Leptin, an immunomodulatory adipokine, functions via leptin receptors, signaling via the long isoform of receptor, LepRb. Leptin promotes DC maturation and increases CCR7 expression on blood DC. Increased mesenteric fat and leptin occur early in Crohn's disease (CD), suggesting leptin-mediated change in intestinal CCR7 expression on DC as a pro-inflammatory mechanism. We have demonstrated CCR7 expression and capacity to migrate to its ligand macrophage inflammatory protein 3β in normal human ileal DC but not colonic or blood DC. In CD, functional CCR7 was expressed on DC from all sites. Only DC populations containing CCR7-expressing cells produced LepRb; in vitro exposure to leptin also increased expression of functional CCR7 in intestinal DC in a dose-dependent manner. In conclusion, leptin may regulate DC migration from gut, in homeostatic and inflammatory conditions, providing a link between mesenteric obesity and inflammation

National prospective cohort study of the burden of acute small bowel obstruction

Background Small bowel obstruction is a common surgical emergency, and is associated with high levels of morbidity and mortality across the world. The literature provides little information on the conservatively managed group. The aim of this study was to describe the burden of small bowel obstruction in the UK. Methods This prospective cohort study was conducted in 131 acute hospitals in the UK between January and April 2017, delivered by trainee research collaboratives. Adult patients with a diagnosis of mechanical small bowel obstruction were included. The primary outcome was in‐hospital mortality. Secondary outcomes included complications, unplanned intensive care admission and readmission within 30 days of discharge. Practice measures, including use of radiological investigations, water soluble contrast, operative and nutritional interventions, were collected. Results Of 2341 patients identified, 693 (29·6 per cent) underwent immediate surgery (within 24 h of admission), 500 (21·4 per cent) had delayed surgery after initial conservative management, and 1148 (49·0 per cent) were managed non‐operatively. The mortality rate was 6·6 per cent (6·4 per cent for non‐operative management, 6·8 per cent for immediate surgery, 6·8 per cent for delayed surgery; P = 0·911). The major complication rate was 14·4 per cent overall, affecting 19·0 per cent in the immediate surgery, 23·6 per cent in the delayed surgery and 7·7 per cent in the non‐operative management groups (P < 0·001). Cox regression found hernia or malignant aetiology and malnutrition to be associated with higher rates of death. Malignant aetiology, operative intervention, acute kidney injury and malnutrition were associated with increased risk of major complication. Conclusion Small bowel obstruction represents a significant healthcare burden. Patient‐level factors such as timing of surgery, acute kidney injury and nutritional status are factors that might be modified to improve outcomes