Energy efficient radio tomographic imaging

pre-printIn this paper, our goal is to develop approaches to reduce the energy consumption in Radio Tomographic Imaging (RTI)-based methods for device free localization without giving up localization accuracy. Our key idea is to only measure those links that are near the current location of the moving object being tracked. We propose two approaches to find the most effective links near the tracked object. In our first approach, we only consider links that are in an ellipse around the current velocity vector of the moving object. In our second approach, we only consider links that cross through a circle with radius r from the current position of the moving object. Thus, rather than creating an attenuation image of the whole area in RTI, we only create the attenuation image for effective links in a small area close to the current location of the moving object. We also develop an adaptive algorithm for determining r. We evaluate the proposed approaches in terms of energy consumption and localization error in three different test areas. Our experimental results show that using our approach, we are able to save 50% to 80% of energy. Interestingly, we find that our radius-based approach actually increases the accuracy of localization

Compound serum and hemin free medium for cultivation of Leishmania tarentolae: A recombinant protein expression system

Serum free cultivation of Leishmania is cost-effective and improves large scale production of well defined parasite material. Moreover, the production of recombinant pharmaceutical proteins requires cultivation of the host in a culture medium free of animal materials, so several culture media for Leishmania tarentolae expression system have been introduced. Some investigations have established the development of a serum-free, but hemin containing medium, based on yeast extract and buffer salts. Hemin is a substance of animal origin also interferes with nickel ions on Ni-NTA resin. In this study, L. tarentolae from Iranian lizard, cultivated in a compound serum and hemin free medium (LBR medium) and the growth parameters were determined. Here we report that LBR medium could obtain high maximal cell density of 1.8 × 108 cells ml-1 equivalent to that of hemin containing medium in our conditions. This compound medium was confirmed by successful expression of a 28 kDa his-tagged protein. With knowledge of the results, the easy-preparing culture medium could be used as a new culture medium for the production of recombinant proteins in L. tarentolae.Keywords: Leishmania tarentolae, serum free cultivation of Leishmania, protein expression, Leishmania culture media

PID controller adjustment for MA-LFC by using imperialist competitive algorithm

In this paper a new evolutionary computing method based on Imperialist Competitive Algorithm (ICA) is used for tuning the elements of a PID controller which is applied in a Multi Area Load Frequency Control System (MA-LFC). If a large power imbalance is suddenly happened in a multi area power electric system, generation units and also consumer sides will be affected by the distortion in the energy balance between both two sides. This inequality is firstly handled by the kinetic energy of the system turning components, but, eventually, the frequency will change. Therefore, LFC is considered as one of the most challenging issues in power system control and operation. PID type controllers are conventional solutions for MA-LFC. The three parameters of the PID controllers have been adjusted traditionally. In this paper, a PID controller is applied for the MA-LFC problem and then its parts are modified by using ICA method. To validate the application of the technique, a multi area network with some uncertainties is provided. Finally the results of the ICAPID controller are compared with the ones of GA optimized controllers. The simulation results show the success and the validity of the ICA-PID controller in compare with the GA - PID controller

Construction Safety Ontology Development and Alignment with Industry Foundation Classes (IFC)

A pronounced gap often exists between expected and actual safety performance in the construction industry. The multifaceted causes of this performance gap are resulting from the misalignment between design assumptions and actual construction processes that take place on-site. In general, critical factors are rooted in the lack of interoperability around the building and work-environment information due to its heterogeneous nature. To overcome the interoperability challenge in safety management, this paper represents the development of an ontological model consisting of terms and relationships between these terms, creating a conceptual information model for construction safety management and linking that ontology to IfcOWL. The developed ontology, named Safety and Health Exchange (SHE), comprises eight concepts and their relationships required to identify and manage safety risks in the design and planning stages. The main concepts of the developed ontology are identified based on reviewing accident cases from 165 Reporting of Injuries, Diseases and Dangerous Occurrences Regulations (RIDDOR) and 31 Press Releases from the database of the Health and Safety Executive (HSE) in the United Kingdom. Consequently, a semantic mapping between the developed ontology and IfcOWL (the most popular ontology and schema for interoperability in the AEC sector) is proposed. Then several SPARQL queries were developed and implemented to evaluate the semantic consistency of the developed ontology and the cross-mapping. The proposed ontology and cross-mapping gained recognition for its innovation in utilising OpenBIM and won the BuildingSMART professional research award 2020. This work could facilitate developing a knowledge-based system in the BIM environment to assist designers in addressing health and safety issues during the design and planning phases in the construction sector

Does HBOC-201 (Hemopure) Affect Platelet Function in Orthopedic Surgery: A Single-Site Analysis from a Multicenter Study

HBOC-201, Hemoglobin glutamer-250 (bovine), (Biopure Corp., Cambridge, MA) has been studied in an international, multicenter, pivotal Phase III trial. A subset analysis of use of blood products indicated that the HBOC-201 group required no more than the packed red blood cell (PRBC) group and was limited to less than 6% in both treatment groups. In a subset analysis from one site, platelet function using PFA-100 was assessed before and after transfusion, and compared those receiving HBOC-201 versus PRBC. After initial IRB approval, patient consent for the Phase III trial and blood draws for PFA-100, an additional IRB exemption for retrospective chart review was obtained. cEPI and cADP means were compared at seven time periods: true baseline(before starting surgery and anesthesia), before transfusion, after transfusion, 1 day, 2 days, 3 to 9 days and 21 or more days after transfusion. Twenty-seven (HBOC: n = 12, PRBC: n = 15) subjects were studied. Comparing data from before transfusion and baseline did not show statistically significant differences in any of cEPI or cADP measurements. cEPI means for the HBOC-201 group increased after transfusion compared to the true baseline (P = 0.01), before transfusion (P = 0.0004) and day 1 after transfusion (P = 0.002). cADP means for the HBOC-201 group were greater after transfusion compared to the true baseline (P = 0.05) and before transfusion (P = 0.005). In the PRBC group there were no significant difference in cEPI and cADP means between all of the time periods. Our study shows that HBOC-201 causes mild platelet dysfunction. Although there were significant changes after HBOC infusion and cEPI and cADP mean values were above the upper normal limit, they did not reach the non-closure time. Further controlled studies are needed to establish definitively the effects that HBOC-201 has on platelet function in patients

The Sorghum bicolor reference genome: improved assembly, gene annotations, a transcriptome atlas, and signatures of genome organization.

Sorghum bicolor is a drought tolerant C4 grass used for the production of grain, forage, sugar, and lignocellulosic biomass and a genetic model for C4 grasses due to its relatively small genome (approximately 800 Mbp), diploid genetics, diverse germplasm, and colinearity with other C4 grass genomes. In this study, deep sequencing, genetic linkage analysis, and transcriptome data were used to produce and annotate a high-quality reference genome sequence. Reference genome sequence order was improved, 29.6 Mbp of additional sequence was incorporated, the number of genes annotated increased 24% to 34 211, average gene length and N50 increased, and error frequency was reduced 10-fold to 1 per 100 kbp. Subtelomeric repeats with characteristics of Tandem Repeats in Miniature (TRIM) elements were identified at the termini of most chromosomes. Nucleosome occupancy predictions identified nucleosomes positioned immediately downstream of transcription start sites and at different densities across chromosomes. Alignment of more than 50 resequenced genomes from diverse sorghum genotypes to the reference genome identified approximately 7.4 M single nucleotide polymorphisms (SNPs) and 1.9 M indels. Large-scale variant features in euchromatin were identified with periodicities of approximately 25 kbp. A transcriptome atlas of gene expression was constructed from 47 RNA-seq profiles of growing and developed tissues of the major plant organs (roots, leaves, stems, panicles, and seed) collected during the juvenile, vegetative and reproductive phases. Analysis of the transcriptome data indicated that tissue type and protein kinase expression had large influences on transcriptional profile clustering. The updated assembly, annotation, and transcriptome data represent a resource for C4 grass research and crop improvement

Acquisition of host-derived carbon in biomass of the ectomycorrhizal fungus Pisolithus microcarpus is correlated to fungal carbon demand and plant defences

Ectomycorrhizal (ECM) fungi are key players in forest carbon (C) sequestration, receiving a substantial proportion of photosynthetic C from their forest tree hosts in exchange for plant growth-limiting soil nutrients. However, it remains unknown whether the fungus or plant controls the quantum of C in this exchange, nor what mechanisms are involved. Here, we aimed to identify physiological and genetic properties of both partners that influence ECM C transfer. Using a microcosm system, stable isotope tracing, and transcriptomics, we quantified plant-to-fungus C transfer between the host plant Eucalyptus grandis and nine isolates of the ECM fungus Pisolithus microcarpus that range in their mycorrhization potential and investigated fungal growth characteristics and plant and fungal genes that correlated with C acquisition. We found that C acquisition by P. microcarpus correlated positively with both fungal biomass production and the expression of a subset of fungal C metabolism genes. In the plant, C transfer was not positively correlated to the number of colonized root tips, but rather to the expression of defence- and stress-related genes. These findings suggest that C acquisition by ECM fungi involves individual fungal demand for C and defence responses of the host against C drain

Effect of Ziziphus jujuba Mill., Cichorium intybus L. And Silybum marianum (L.) Gaertn. Combination extract on non-alcoholic fatty liver disease in rats

Background: Non-alcoholic fatty liver disease (NAFLD) includes a range of liver damage from early steatosis to cirrhosis. Although NAFLD prevalence in the world is increasing, but there is no effective treatment for it. Objective: The aim of this study was to evaluate the effect of combined extract of jujube, chicory and silymarin on NAFLD induced by high-fat diet (HFD) in rats. Method: In this experimental study 40 male rats randomly were divided in two groups: a negative control group (n = 8) and a high-fat diet group (n = 32). After 4 months of feeding with HFD, rats were assigned into 4 groups (n = 8) including an HFD group and three groups receiving HFD and the extract at 100, 200 and 400 mg/kg for 2 months. Finally, lipid profile, liver enzymes activity and liver histology were investigated. Results: High-fat diet increased cholesterol, triglyceride and LDL and decreased HDL levels (P<0.001). This diet also increased serum activity hepatic enzymes and lipid accumulation in liver tissue. Receiving the extract improved lipid profile and hepatic enzyme activity, dose-dependently. Histopathology of liver confirmed the change induced by HFD and protective effect of extract. Conclusion: Treatment with combined extracts of jujube, chicory and silymarin improves high-fat diet (HFD) induced NAFLD in rats. © 2020 Institute of Medicinal Plants. All rights reserved

The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases.

yesSunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids and their subsequent metabolism by cyclooxygenases (COX) and lipoxygenases (LOX) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and for 72h examined (i) expression of pro- and anti-inflammatory eicosanoids using LC/ESI-MS/MS and (ii) immunohistochemical expression of COX-2, 12-LOX, 15-LOX and leucocyte markers, while (iii) quantifying clinical erythema. We show that vasodilatory prostaglandins (PG)E2, PGF2¿ and PGE3 accompany the erythema in the first 24-48h, associated with increased COX-2 expression at 24h. Novel, potent leukocyte chemoattractants 11-, 12- and 8-monohydroxy-eicosatetraenoic acid (-HETE) are elevated from 4-72h, in association with peak dermal neutrophil influx at 24h, and increased dermal CD3+ lymphocytes and 12- and 15-LOX expression from 24-72h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72h. Sunburn is characterized by overlapping phases of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.The Wellcome Trus