Social Opportunity Causes Rapid Transcriptional Changes in the Social Behaviour Network of the Brain in an African Cichlid Fish

Animals constantly integrate external stimuli with their own internal physiological state to make appropriate behavioural decisions. Little is known, however, about where in the brain the salience of these signals is evaluated, or which neural and transcriptional mechanisms link this integration to adaptive behaviours. We used an African cichlid fish Astatotilapia burtoni to test the hypothesis that a new social opportunity activates the conserved \u27social behaviour network\u27 (SBN), a collection of brain nuclei known to regulate social behaviours across vertebrates. We measured mRNA levels of immediate early genes (IEGs) in microdissected brain regions as a proxy for neuronal activation, and discovered that IEGs were higher in all SBN nuclei in males that were given an opportunity to rise in social rank compared to control stable subordinate and dominant individuals. Furthermore, because the presence of sex-steroid receptors is one defining criteria of SBN nuclei, we also tested whether social opportunity or status influenced androgen and oestrogen receptor mRNA levels within these same regions. There were several rapid region-specific changes in receptor mRNA levels induced by social opportunity, most notably in oestrogen receptor subtypes in areas that regulate social aggression and reproduction, suggesting that oestrogenic signalling pathways play an important role in regulating male status. Several receptor mRNA changes occurred in regions with putative homologies to the mammalian septum and extended amygdala, two regions shared by SBN and reward circuits, suggesting an important role in the integration of social salience, stressors, hormonal state and adaptive behaviours. We also demonstrated increases in plasma sex- and stress-steroids at 30 min after a rise in social rank. This rapid endocrine and transcriptional response suggests that the SBN is involved in the integration of social inputs with internal hormonal state to facilitate the transition to dominant status, which ultimately leads to improved fitness for the previously reproductively-suppressed individual. © 2012 British Society for Neuroendocrinology

Muonium as a shallow center in GaN

A paramagnetic muonium (Mu) state with an extremely small hyperfine parameter was observed for the first time in single-crystalline GaN below 25 K. It has a highly anisotropic hyperfine structure with axial symmetry along the [0001] direction, suggesting that it is located either at a nitrogen-antibonding or a bond-centered site oriented parallel to the c-axis. Its small ionization energy (=< 14 meV) and small hyperfine parameter (--10^{-4} times the vacuum value) indicate that muonium in one of its possible sites produces a shallow state, raising the possibility that the analogous hydrogen center could be a source of n-type conductivity in as-grown GaN.Comment: 4 figures, to be published in Phys. Rev. Letter

Nitrogen ion beam synthesis of InN in InP (100) at elevated temperature

InN phase is grown in crystalline InP(100) substrates by 50 keV N+ implantation at an elevated temperature of 400 deg C followed by annealing at 525 deg C in N2 ambient. Crystallographic structural and Raman scattering studies are performed for the characterization of grown phases. Temperature- and power-dependent photoluminescence studies show direct band-to-band transition peak ~1.06 eV at temperatures <=150K. Implantations at an elevated temperature with a low ion beam current and subsequent low temperature annealing step are found responsible for the growth of high-quality InN phase.Comment: 11 pages, 4 figures, Journa

Transcriptomic response to aquaculture intensification in Nile tilapia

To meet future global demand for fish protein, more fish will need to be farmed usingfewer resources, and this will require the selection of nonaggressive individuals thatperform well at high densities. Yet, the genetic changes underlying loss of aggressionand adaptation to crowding during aquaculture intensification are largely unknown.We examined the transcriptomic response to aggression and crowding in Nile tilapia,one of the oldest and most widespread farmed fish, whose social structure shiftsfrom social hierarchies to shoaling with increasing density. A mirror test was usedto quantify aggression and skin darkening (a proxy for stress) of fish reared at lowand high densities, and gene expression in the hypothalamus was analysed amongthe most and least aggressive fish at each density. Fish reared at high density weredarker, had larger brains, were less active and less aggressive than those reared atlow density and had differentially expressed genes consistent with a reactive stress-copingstyle and activation of the hypothalamus–pituitary–interrenal (HPI) axis.Differences in gene expression among aggressive fish were accounted for by densityand the interaction between density and aggression levels, whereas for non-aggressivefish differences in gene expression were associated with individual variation inskin brightness and social stress. Thus, the response to crowding in Nile tilapia iscontext dependent and involves different neuro-endocrine pathways, depending onsocial status. Knowledge of genes associated with the response to crowding maypave the way for more efficient fish domestication, based on the selection of non-aggressiveindividuals with increasing tolerance to chronic stress necessary for aquacultureintensification

Gaia Data Release 2: Properties and validation of the radial velocities

Context. For Gaia DR2, 280 million spectra collected by the Radial Velocity Spectrometer instrument on board Gaia were processed, and median radial velocities were derived for 9.8 million sources brighter than GRVS = 12 mag. Aims. This paper describes the validation and properties of the median radial velocities published in Gaia DR2. Methods. Quality tests and filters were applied to select those of the 9.8 million radial velocities that have the quality to be published in Gaia DR2. The accuracy of the selected sample was assessed with respect to ground-based catalogues. Its precision was estimated using both ground-based catalogues and the distribution of the Gaia radial velocity uncertainties. Results. Gaia DR2 contains median radial velocities for 7 224 631 stars, with Teff in the range [3550, 6900] K, which successfully passed the quality tests. The published median radial velocities provide a full-sky coverage and are complete with respect to the astrometric data to within 77.2% (for G larger than or equal to 12.5 mag). The median radial velocity residuals with respect to the ground-based surveys vary from one catalogue to another, but do not exceed a few 100 m s-1. In addition, the Gaia radial velocities show a positive trend as a function of magnitude, which starts around GRVS - 9 mag and reaches about +500 m s-1 at GRVS = 11.75 mag. The origin of the trend is under investigation, with the aim to correct for it in Gaia DR3. The overall precision, estimated from the median of the Gaia radial velocity uncertainties, is 1.05 km s-1. The radial velocity precision is a function of many parameters, in particular, the magnitude and effective temperature. For bright stars, GRVS E [4, 8] mag, the precision, estimated using the full dataset, is in the range 220-350 m s-1, which is about three to five times more precise than the pre-launch specification of 1 km s-1. At the faint end, GRVS = 11.75 mag, the precisions for Teff = 5000 and 6500 K are 1.4 and 3.7 km s-1, respectively.Funding for the DPAC has been provided by national institutions, in particular the institutions participating in the Gaia Multilateral Agreement. Most of the authors are current or past members of the ESA Gaia mission team and of the Gaia DPAC and their work has been supported by the French Centre National de la Recherche Scientifique (CNRS), the Centre National d’Etudes Spatiales (CNES), the Agence Nationale de la Recherche, the Région Aquitaine, the Université de Bordeaux, the Utinam Institute of the Université de Franche-Comté, and the Institut des Sciences de l’ Univers (INSU); the Science and Technology Facilities Council and the United Kingdom Space Agency; the Belgian Federal Science Policy Office (BELSPO) through various Programme de Développement d’Expériences Scientifiques (PRODEX) grants; the German Aerospace Agency (Deutsches Zentrum fur Luft- und Raumfahrt e.V., DLR); the Algerian Centre de Recherche en Astronomie, Astrophysique et Géophysique of Bouzareah Observatory; the Swiss State Secretariat for Education, Research, and Innovation through the ESA PRODEX programme, the Mesures d’Accompagnement, the Swiss Activités Nationales Complémentaires, and the Swiss National Science Foundation; the Slovenian Research Agency (research core funding No. P1-0188

Visual Information Alone Changes Behavior and Physiology during Social Interactions in a Cichlid Fish (Astatotilapia burtoni)

Social behavior can influence physiological systems dramatically yet the sensory cues responsible are not well understood. Behavior of male African cichlid fish, Astatotilapia burtoni, in their natural habitat suggests that visual cues from conspecifics contribute significantly to regulation of social behavior. Using a novel paradigm, we asked whether visual cues alone from a larger conspecific male could influence behavior, reproductive physiology and the physiological stress response of a smaller male. Here we show that just seeing a larger, threatening male through a clear barrier can suppress dominant behavior of a smaller male for up to 7 days. Smaller dominant males being “attacked” visually by larger dominant males through a clear barrier also showed physiological changes for up to 3 days, including up-regulation of reproductive- and stress-related gene expression levels and lowered plasma 11-ketotestesterone concentrations as compared to control animals. The smaller males modified their appearance to match that of non-dominant males when exposed to a larger male but they maintained a physiological phenotype similar to that of a dominant male. After 7 days, reproductive- and stress- related gene expression, circulating hormone levels, and gonad size in the smaller males showed no difference from the control group suggesting that the smaller male habituated to the visual intruder. However, the smaller male continued to display subordinate behaviors and assumed the appearance of a subordinate male for a full week despite his dominant male physiology. These data suggest that seeing a larger male alone can regulate the behavior of a smaller male but that ongoing reproductive inhibition depends on additional sensory cues. Perhaps, while experiencing visual social stressors, the smaller male uses an opportunistic strategy, acting like a subordinate male while maintaining the physiology of a dominant male

Social information changes the brain

Social animals live in complex physical and social environments requiring them to attend and rapidly respond to social and environmental information by changing their behavior. A key social influence is rank or status, a ubiquitous element in animal societies. Rank typically regulates access to reproduction and other resources, among other consequences for individuals. Because reproduction is arguably the most important event in any animals’ life, understanding how reproduction is regulated by social status and related physiological factors can instruct our understanding of evolutionary change. This article reviews evidence from a model social system in which reproduction is tightly controlled by social status. Surprisingly, changes in social status have rapid and profound effects over very short time scales and radically alter overt behavior, as well as physiological, cellular, and molecular factors that regulate reproductive capacity

Dual Roles of an Algal Farming Damselfish as a Cultivator and Opportunistic Browser of an Invasive Seaweed

Abstract Herbivory is a fundamental process determining reef resilience, and while algal farming damselfishes can help shape benthic assemblages, an understanding of their contribution to areas outside of defended territories is relatively unexplored. Here, we demonstrate how the farming damselfish Stegastes marginatus plays a dual role in benthic structuring by 1) contributing to persistence of the invasive macroalga Acanthophora spicifera within a Hawaiian marine protected area, where the macroalga occurred exclusively inside Stegastes territories, and 2) behaving as an opportunistic browser of the exotic alga outside their territorial borders. Greater than 50% of the biomass of tethered A. spicifera was consumed within one-hour when placed outside Stegastes territories, compared to ,5% lost from tethers within territories or herbivore exclusion cages. In situ remote video revealed that tethered A. spicifera located outside territories was grazed primarily by the surgeonfish Acanthurus nigrofuscus (,68% of total bites) and, surprisingly, by S. marginatus (,27% of total bites) that left their territories to feed on this resource on 107 occasions during 400 min of filming. Further, for over half of those occurrences where S. marginatus grazed on the tethered macroalga outside of territories, they fed alongside conspecifics and other species, displaying little of the aggressiveness that characterizes this damselfish. These results show that S. marginatus plays a wider role in determining benthic assemblages than previously recognized, acting both as cultivators of a canopy-forming invasive macroalga within their territories, and as opportunistic browsers in neighboring sites. Consequently, S. marginatus can affect benthic species composition across their territory borders. These results provide a rare example of interspecific facilitation of an exotic alga by an indigenous marine fish. Accounting for fish behaviors more broadly is important to further our understanding of ecological processes that shape reef ecosystems to improve management of MPAs that often support extensive farming damselfish populations

Social Regulation of Male Reproductive Plasticity in an African Cichlid Fish

Social interactions with the outcome of a position in a dominance hierarchy can have profound effects on reproductive behavior and physiology, requiring animals to integrate environmental information with their internal physiological state; but how is salient information from the animal’s dynamic social environment transformed into adaptive behavioral, physiological, and molecular-level changes? The African cichlid fish, Astatotilapia burtoni, is ideally suited to understand socially controlled reproductive plasticity because activity of the male reproductive (brain–pituitary–gonad) axis is tightly linked to social status. Males form hierarchies in which a small percentage of brightly colored dominant individuals have an active reproductive axis, defend territories, and spawn with females, while the remaining males are subordinate, drably colored, do not hold a territory, and have a suppressed reproductive system with minimal opportunities for spawning. These social phenotypes are plastic and quickly reversible, meaning that individual males may switch between dominant and subordinate status multiple times within a lifetime. Here, we review the rapid and remarkable plasticity that occurs along the entire reproductive axis when males rise in social rank, a transition that has important implications for the operational sex ratio of the population. When males rise in rank, transformations occur in the brain, pituitary, circulation, and testes over short time-scales (minutes to days). Changes are evident in overt behavior, as well as modifications at the physiological, cellular, and molecular levels that regulate reproductive capacity. Widespread changes triggered by a switch in rank highlight the significance of external social information in shaping internal physiology and reproductive competence