Electrical responses of oligodendrocytes to pathological stimuli

The white matter is crucial for rapid transmission of information between different parts of the brain and spinal cord, and is damaged in diseases including the genetic leukodystrophies, stroke, spinal cord injury and multiple sclerosis. While damage in the grey matter of the CNS is well known to often involve over-activation of glutamate receptors, our understanding of white matter pathology is less advanced. The experiments in this thesis used patch-clamping and [Ca2+]i imaging to examine the cerebellar white matter oligodendrocyte response to pathological insults mimicking those occurring in the leukodystrophies and in the ischaemia that occurs in stroke or after spinal cord injury. Oligodendrocytes responded to simulated ischaemia with an inward current, which was triggered by glutamate release mediated by reversal of glutamate transporters, and not by exocytosis, NKCC1 or cystine/glutamate exchange. Surprisingly, this inward current was not mediated by glutamate receptors, nor by ASICs, gap junctional hemichannels, P2X receptors or GABAA receptors, but reflected the closing of potassium channels. In current clamp mode this initial closing of K+ channels produced a depolarisation of the cells, followed by a repolarisation as other K+ channels activated. These data indicate, for the first time, a significant role for K+ channels in the response of oligodendrocytes to ischaemia. In Canavan and Pelizaeus-Merzbacher-like leukodystrophies, elevated levels of Nacetylaspartylglutamate (NAAG) and N-acetylaspartate (NAA) occur. These compounds can activate or block neuronal NMDA receptors. Since oligodendrocytes are reported to express NMDA receptors, I tested their response to NAAG and NAA. NAAG, but not NAA, evoked a small inward NMDA receptor-mediated current in oligodendrocytes, but no [Ca2+]i rise. Much of the inward current was a secondary effect of NAAG acting on neurons. Thus, actions of NAAG and NAA on oligodendrocyte NMDARs are unlikely to be a major contributor to white matter damage in the leukodystrophies

Imaging properties of the light sword optical element used as a contact lens in a presbyopic eye model

The paper analyzes the imaging properties of the light sword optical element (LSOE) applied as a contact lens to the presbyopic human eye. We performed our studies with a human eye model based on the Gullstrand parameterization. In order to quantify the discussion concerning imaging with extended depth of focus, we introduced quantitative parameters characterizing output images of optotypes obtained in numerical simulations. The quality of the images formed by the LSOE were compared with those created by a presbyopic human eye, reading glasses and a quartic inverse axicon. Then we complemented the numerical results by an experiment where a 3D scene was imaged by means of the refractive LSOE correcting an artificial eye based on the Gullstrand model. According to performed simulations and experiments the LSOE exhibits abilities for presbyopia correction in a wide range of functional vision distances.This work was supported by the Polish Ministry of Science and Higher Education under grant N N 514 149038 and by the Spanish Ministerio de Ciencia e Innovación (MICINN), grant FIS2008-03884 with complementary support from the European Social Fund implemented under the Human Capital Programme (POKL), project, “Preparation and Realization of Medical Physics Specialty.”S

Strehl ratios characterizing optical elements designed for presbyopia compensation

We present results of numerical analysis of the Strehl ratio characteristics for the light sword optical element (LSOE). For comparison there were analyzed other optical imaging elements proposed for compensation of presbyopia such as the bifocal lens, the trifocal lens, the stenopeic contact lens, and elements with extended depth of focus (EDOF), such as the logarithmic and quartic axicons. The simulations were based on a human eye’s model being a simplified version of the Gullstrand model. The results obtained allow to state that the LSOE exhibits much more uniform characteristics of the Strehl ratio comparing with other known hitherto elements and therefore it could be a promising aid to compensate for the insufficient accommodation range of the human eye.This work was supported by the Polish Ministry of Science and Higher Education under grants N N 514 149038 and N N 518 378237 as well as by the European Social Fund implemented under the Human Capital Operational Programme (POKL), project: “Preparation and Realization of Medical Physics Specialty” with complementary support from the Spanish Ministerio de Ciencia e Innovación (MICINN) grant FIS2008-03884S

Imaging with extended focal depth by means of the refractive light sword optical element

The paper presents first experiments with a refractive light sword optical element (LSOE). A refractive version of the LSOE was prepared in photoresist by gray scale photolithography. Then we examined chromatic aberrations of the produced element and compared them with those corresponding to two different lenses. For this purpose we performed two experiments, the first one where white light illumination was used and the latter one by the help of monochromatic illumination with three different wavelengths. The obtained results lead to the conclusion that the refractive LSOE does not exhibit significant chromatic aberrations and can be successfully used for imaging with extended depth of focus in polychromatic illuminationThis work was supported by Warsaw University of Technology, by National Institute of Telecommunications and by the Spanish Ministerio de Educación y Ciencia, Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i), grant FIS2005-05020- C03-02 and FEDERS

The oxidizing power of the dark side: Rapid nocturnal aging of biomass burning as an overlooked source of oxidized organic aerosol

Oxidized organic aerosol (OOA) is a major component of ambient particulate matter, substantially affecting both climate and human health. A considerable body of evidence has established that OOA is readily produced in the presence of daylight, thus leading to the association of high concentrations of OOA in the summer or mid-afternoon. However, this current mechanistic understanding fails to explain elevated OOA concentrations during night or wintertime periods of low photochemical activity, thus leading atmospheric models to under predict OOA concentrations by a factor of 3-5. Here we show that fresh emissions from biomass burning rapidly forms OOA in the laboratory over a few hours and without any sunlight. The resulting OOA chemical composition is consistent with the observed OOA in field studies in major urban areas. To estimate the contribution of nocturnally aged OOA in the ambient atmosphere, we incorporate this nighttime-aging mechanism into a chemical-transport model and find that over much of the United States greater than 75% of the OOA formed from fresh biomass burning emissions underwent nighttime aging processes. Thus, the conceptual framework that OOA is predominantly formed in the presence of daylight fails to account for a substantial and rapid oxidation process occurring in the dark

The effect of N-acetyl-aspartyl-glutamate and N-acetyl-aspartate on white matter oligodendrocytes

Elevations of the levels of N-acetyl-aspartyl-glutamate (NAAG) and N-acetyl-aspartate (NAA) are associated with myelin loss in the leucodystrophies Canavan's disease and Pelizaeus-Merzbacher-like disease. NAAG and NAA can activate and antagonize neuronal N-methyl-D-aspartate (NMDA) receptors, and also act on group II metabotropic glutamate receptors. Oligodendrocytes and their precursors have recently been shown to express NMDA receptors, and activation of these receptors in ischaemia leads to the death of oligodendrocyte precursors and the loss of myelin. This raises the possibility that the failure to develop myelin, or demyelination, occurring in the leucodystrophies could reflect an action of NAAG or NAA on oligodendrocyte NMDA receptors. However, since the putative subunit composition of NMDA receptors on oligodendrocytes differs from that of neuronal NMDA receptors, the effects of NAAG and NAA on them are unknown. We show that NAAG, but not NAA, evokes an inward membrane current in cerebellar white matter oligodendrocytes, which is reduced by NMDA receptor block (but not by block of metabotropic glutamate receptors). The size of the current evoked by NAAG, relative to that evoked by NMDA, was much smaller in oligodendrocytes than in neurons, and NAAG induced a rise in [Ca^{2+}]i in neurons but not in oligodendrocytes. These differences in the effect of NAAG on oligodendrocytes and neurons may reflect the aforementioned difference in receptor subunit composition. In addition, as a major part of the response in oligodendrocytes was blocked by tetrodotoxin (TTX), much of the NAAG-evoked current in oligodendrocytes is a secondary consequence of activating neuronal NMDA receptors. Six hours exposure to 1 mM NAAG did not lead to the death of cells in the white matter. We conclude that an action of NAAG on oligodendrocyte NMDA receptors is unlikely to be a major contributor to white matter damage in the leucodystrophies

Certainty relations between local and nonlocal observables

We demonstrate that for an arbitrary number of identical particles, each defined on a Hilbert-space of arbitrary dimension, there exists a whole ladder of relations of complementarity between local, and every conceivable kind of joint (or nonlocal) measurements. E.g., the more accurate we can know (by a measurement) some joint property of three qubits (projecting the state onto a tripartite entangled state), the less accurate some other property, local to the three qubits, become. We also show that the corresponding complementarity relations are particularly tight for particles defined on prime dimensional Hilbert spaces.Comment: 4 pages, no figure

Contrast transfer characteristics of the light sword optical element designed for presbyopia compensation

The paper discusses the abilities of the light sword optical element (LSOE) for presbyopia compensation. The imaging properties are analyzed by means of the modulation transfer functions and output images of the star resolution test. All numerical calculations are performed assuming an optical set-up simulating the presbyopic human eye and based on the Gullstrand model. In order to have a meaningful comparison we expand our study and present adequate analysis for other elements potentially useful in ophthalmology as reading glasses, bifocal lenses and axicons. According to the obtained results the LSOE can successfully realize vision with an extended depth of field. The element makes possible the compensation of an assumed defocus up to 4 dioptres. The output images formed by the LSOE are well recognizable and have acceptable qualities for near as well as far object distances

Experimental study and analysis of lubricants dispersed with nano Cu and TiO2 in a four-stroke two wheeler

The present investigation summarizes detailed experimental studies with standard lubricants of commercial quality known as Racer-4 of Hindustan Petroleum Corporation (India) dispersed with different mass concentrations of nanoparticles of Cu and TiO2. The test bench is fabricated with a four-stroke Hero-Honda motorbike hydraulically loaded at the rear wheel with proper instrumentation to record the fuel consumption, the load on the rear wheel, and the linear velocity. The whole range of data obtained on a stationery bike is subjected to regression analysis to arrive at various relationships between fuel consumption as a function of brake power, linear velocity, and percentage mass concentration of nanoparticles in the lubricant. The empirical relation correlates with the observed data with reasonable accuracy. Further, extension of the analysis by developing a mathematical model has revealed a definite improvement in brake thermal efficiency which ultimately affects the fuel economy by diminishing frictional power in the system with the introduction of nanoparticles into the lubricant. The performance of the engine seems to be better with nano Cu-Racer-4 combination than the one with nano TiO2

Network adaptation improves temporal representation of naturalistic stimuli in drosophila eye: II Mechanisms

Retinal networks must adapt constantly to best present the ever changing visual world to the brain. Here we test the hypothesis that adaptation is a result of different mechanisms at several synaptic connections within the network. In a companion paper (Part I), we showed that adaptation in the photoreceptors (R1-R6) and large monopolar cells (LMC) of the Drosophila eye improves sensitivity to under-represented signals in seconds by enhancing both the amplitude and frequency distribution of LMCs' voltage responses to repeated naturalistic contrast series. In this paper, we show that such adaptation needs both the light-mediated conductance and feedback-mediated synaptic conductance. A faulty feedforward pathway in histamine receptor mutant flies speeds up the LMC output, mimicking extreme light adaptation. A faulty feedback pathway from L2 LMCs to photoreceptors slows down the LMC output, mimicking dark adaptation. These results underline the importance of network adaptation for efficient coding, and as a mechanism for selectively regulating the size and speed of signals in neurons. We suggest that concert action of many different mechanisms and neural connections are responsible for adaptation to visual stimuli. Further, our results demonstrate the need for detailed circuit reconstructions like that of the Drosophila lamina, to understand how networks process information