The politics of playing along: radio and regulation in democratic Europe

The loss of localism has been a common trend in most radio markets in Europe and the United States. Deregulation of ownership led to a concentration phenomenon that has affected local radios in several western democracies. New forms of regulation have been considered as possible ways to stop the erosion of localism by applying rules to markets in the defense of diversity, pluralism, and the local dimension. This article analyses the local radio market in Portugal, by assessing the changes in the legislation and by examining the regulatory action that can, according to its remit, act preventively to avoid market forces to harm these principles.info:eu-repo/semantics/publishedVersio

Poland's 2011 Online Election Campaign: New Tools, New Professionalism, New Ways to Win Votes

This article analyzes the use of the online environment within the context of the Polish parliamentary election of 2011. Using traditional methods of content analysis, we find that parties tend to adhere to a professionalized model of campaigning, and adapting online tools to suit the objectives of the campaign. There also appears to be a recognition that their most likely visitors to these online presences would be converts, and so they attempt to mobilize supporters rather than convert browsers. New parties and candidates are more likely to target browsers, with the latter offering a more personalized experience to online visitors. Importantly, when analyzing the outcome of the contest, we find that being online matters for candidates when controlling for all other variables. Equally, the reach the candidate has, which may well influence their vote share, is dependent on offering a more personalized, representational image and having a frequently updated online presence that should encourage repeat visits. Cumulatively, we suggest the future of online campaigning must not only focus on having a presence, but on using it in a way that appeals to a range of visitors, encouraging repeat visits, and that this strategy could have a positive impact on election outcomes. © Taylor & Francis Group, LLC

Unusual shallow water Devonian coral community from Queensland and its recent analogues from the inshore Great Barrier Reef

Palaeozoic coral communities were dominated by two extinct coral groups: Tabulata and Rugosa. Whilst they are not closely related to modern Scleractinia, they are morphologically convergent, displaying many morphological characters that allow comparisons between recent and ancient coral reef communities. The extensive shallow-water reef communities of the Devonian were generally dominated by stromatoporoid sponges, with corals occupying deeper environments. Here, we describe an unusual, shallow water coral reef community from the Middle Devonian (Givetian, approx. 385 Ma) of the Fanning River area, Queensland, Australia. The coral community is dominated by tabulate corals, but also includes solitary and occasionally colonial rugose corals. Tabulate corals most commonly exhibit foliose and massive morphologies, but encrusting and branching growth forms also occur. The depositional environment was characterized by a shallow water depth, moderate hydrodynamic energy, high sedimentation rate, and high turbidity. Since these environmental factors influence the morphological composition of modern coral communities, we hypothesize that similar environments may result in morphologically equivalent coral assemblages throughout the Phanerozoic. To test this idea, we qualitatively compare the Fanning River reefs with modern scleractinian coral assemblages in a similar environmental setting at Magnetic Island. Both reefs are located in a shallow water less than 10 m deep, with high sediment flux, moderate wave energy, and generally high turbidity. Like Fanning River, Magnetic Island coral communities are dominated by foliose morphologies, with contributions from massive and branching forms. The Fanning River reef, together with previously identified Silurian and Devonian mesophotic coral ecosystems, suggest that Palaeozoic coral assemblages may share many functional characteristics with modern scleractinian reefs in similar environments. Therefore, the geological record of inshore, high turbidity-adapted coral communities can be traced back as far as 385 Ma

74 Ocena skuteczności leczenia przerzutów odległych u chorych na miejscowo wyleczonego raka szyjki macicy

Cel pracyOcena skuteczności leczenia przerzutów odległych u chorych na miejscowo wyleczonego raka szyjki macicy.MetodykaRetrospektywna analiza materiału obejmującego 113 chorych na miejscowo wyleczonego RSM, u których wystąpiły przerzuty odległe. Wszystkie chore badanej grupy były leczone w Krakowskim Oddziale Centrum Onkologii (COOK) w latach 1986–1992. Oceniono wpływ leczenia chirurgicznego na przeżycie chorych oraz efekt paliatywny tego leczenia.WynikiNajczęstszą lokalizacją pierwszego lub pierwszych przerzutów były płuca (37 chorych), kości (26 chorych) i wątroba (20 chorych); ponadto stwierdzono przerzuty do mózgu (11 chorych), obwodowych węzłów chłonnych (11 chorych) i skóry (8 chorych). U [[mml:math altimg="si1.gif"]][[mml:mrow]][[mml:mstyle scriptlevel="1"]][[mml:mfrac bevelled="true"]][[mml:mn]]3[[/mml:mn]][[mml:mn]]4[[/mml:mn]][[/mml:mfrac]][[/mml:mstyle]][[/mml:mrow]][[/mml:math]] chorych stwierdzono przerzuty pojedyncze, u 1/4 mnogie. W ciągu pierwszego roku po leczeniu miejscowym, przerzuty odległe wystąpiły u 42,5% chorych, w ciągu 3 lat u ponad [[mml:math altimg="si1.gif"]][[mml:mrow]][[mml:mstyle scriptlevel="1"]][[mml:mfrac bevelled="true"]][[mml:mn]]3[[/mml:mn]][[mml:mn]]4[[/mml:mn]][[/mml:mfrac]][[/mml:mstyle]][[/mml:mrow]][[/mml:math]] chorych; średni czas wystąpienia przerzutów wynosił 23 miesiące. Spośród 113 chorych badanej grupy, leczenie przyczynowe przerzutów odległych podjęto jedynie u 48 tzn. 42,5% pacjentek; u 3 chorych zastosowano leczenie operacyjne (metastazektomia pojedynczych przerzutów do płuc), u 20 wyłącznie teleradioterapię, u kolejnych 20 wyłącznie chemioterapię, u 5 skojarzenie chemioradioterapii. Od momentu wystąpienia przerzutów odlegfych,odległych, 12 miesięcy przeżyło jedynie 15% chorych badanej grupy, a 24 miesiące 4% chorych.Wnioski1.Zarówno telaradioterapia jak i chemioterapia oparta na cysplatynie z 5-fluorouracylem lub karboplatynie, nie miały statystycznie znamiennego wpływu na przeżycia chorych badanej grupy.2.Telaradioterapia jest skuteczną metodą łagodzenia dolegliwości u chorych z przerzutami RSM do kości, obwodowych węzłów chłonnych i skóry

Small molecule sensitization to TRAIL is mediated via nuclear localization, phosphorylation and inhibition of chaperone activity of Hsp27

10.1038/cddis.2013.413Cell Death and Disease410

Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance

A Saccharomyces cerevisiae strain in which the GPP1 and GPP2 genes, both encoding glycerol-3-phosphate phosphatase isoforms, are deleted, displays both osmo- and thermosensitive (ts) phenotypes. We isolated genes involved in cell wall maintenance as multicopy suppressors of the gpp1gpp2 ts phenotype. We found that the gpp1gpp2 strain is hypersensitive to cell wall stress such as treatment with β-1,3-glucanase containing cocktail Zymolyase and chitin-binding dye Calcofluor-white (CFW). Sensitivity to Zymolyase was rescued by overexpression of SSD1, while CFW sensitivity was rescued by SSD1, FLO8 and WSC3-genes isolated as multicopy suppressors of the gpp1gpp2 ts phenotype. Some of the isolated suppressor genes (SSD1, FLO8) also rescued the lytic phenotype of slt2 deletion strain. Additionally, the sensitivity to CFW was reduced when the cells were supplied with glycerol. Both growth on glycerol-based medium and overexpression of SSD1, FLO8 or WSC3 had additive suppressing effect on CFW sensitivity of the gpp1gpp2 mutant strain. We also confirmed that the internal glycerol level changed in cells exposed to cell wall perturbation. © 2007 Springer-Verlag

Corrosion behaviour of porous Ti intended for biomedical applications

Porous Ti implants are being developed inorder to reduce the biomechanical mismatch between theimplant and the bone, as well as increasing the osseointegrationby improving the bone in-growth. Most of the focusin the literature has been on the structural, biological andmechanical characterization of porous Ti whereas there islimited information on the electrochemical characterization.Therefore, the present work aims to study the corrosionbehaviour of porous Ti having 30 and 50 % ofnominal porosity, produced by powder metallurgy routeusing the space holder technique. The percentage, size anddistribution of the pores were determined by image analysis.Electrochemical tests consisting of potentiodynamicpolarization and electrochemical impedance spectroscopywere performed in 9 g/L NaCl solution at body temperature.Electrochemical studies revealed that samples presenteda less stable oxide film at increased porosity, morespecifically, the complex geometry and the interconnectivityof the pores resulted in formation of less protectiveoxide film in the pores.This study was supported by FCT with the reference project UID/EEA/04436/2013, by FEDER funds through the COMPETE 2020 – Programa Operacional Competitividade e Internacionalizac¸a˜o (POCI) with the reference project POCI-01-0145- FEDER-006941, Programa de Acc¸o˜es Universita´rias Integradas LusoFrancesas’ (PAUILF TC-12_14), and The Calouste Gulbenkian Foundation through ‘‘Programa de Mobilidade Acade´mica para Professores’’. The authors would also like to acknowledge Prof. Ana Senos (University of Aveiro) and Prof. Jose´ Carlos Teixeira (University of Minho) for the provision of the characterization facilities.info:eu-repo/semantics/publishedVersio

The Role of Bcl-2 and Beclin-1 Complex in “Switching” between Apoptosis and Autophagy in Human Glioma Cells upon LY294002 and Sorafenib Treatment

Background: Gliomas are the most malignant tumors of the central nervous system. One of the factors in their high drug resistance is avoiding programmed death (PCD) induction. This is related to the overexpression of intracellular survival pathways: PI3K-Akt/PKB-mTOR and Ras-Raf-MEK-ERK. Apoptosis and autophagy are co-existing processes due to the interactions between Bcl-2 and beclin-1 proteins. Their complex may be a molecular “toggle-switch” between PCD types. The aim of this research was to investigate the role of Bcl-2:beclin-1 complex in glioma cell elimination through the combined action of LY294002 and sorafenib. Methods: Drug cytotoxicity was estimated with an MTT test. The type of cell death was evaluated using variant microscopy techniques (fluorochrome staining, immunocytochemistry, and transmission electron microscopy), as well as the Bcl-2:beclin-1 complex formation and protein localization. Molecular analysis of PCD indicators was conducted through immunoblotting, immunoprecipitation, and ELISA testing. SiRNA was used to block Bcl-2 and beclin-1 expression. Results: The results showed the inhibitors used in simultaneous application resulted in Bcl-2:beclin-1 complex formation and apoptosis becoming dominant. This was accompanied by changes in the location of the tested proteins. Conclusions: “Switching” between apoptosis and autophagy using PI3K and Raf inhibitors with Bcl-2:beclin-1 complex formation opens new therapeutic perspectives against gliomas

5 Rak endometrium – wstępna ocena tolerancji pooperacyjnej pulsacyjnej brachyterapii

CelOcena tolerancji dopochwowego leczenia PDR (pulsed dose-rate) chorych operowanych z powodu raka trzonu macicy.Materiał i MetodaU 110 chorych operowanych z powodu raka trzonu macicy przeprowadzono po raz pierwszy w Polsce w okresie od 1.10.1998 do 31.12.1999 roku uzupełniające dopochwowe leczenie techniką PDR. Chore leczone były cylindrycznym aplikatorem dopochwowym, otrzymywały 21 Gy w ciągu 21 godzin leczenia (dawka 1 Gy/h, czas trwania pulsu od 10 do 30 minut w związku ze spadkiem aktywności źródła irydowego). Dawkę obliczano 0,5 cm od powierzchni aplikatora oraz 1cm od szczytu pochwy. U każdej chorej indywidualnie obliczono rozkład dawki w terenie miednicy przy użyciu IBU oraz systemu Plato. U 43 chorych (39%) PDR był jedyną formą uzupełniającej radioterapii, u 67 chorych (61%) zastosowano dodatkowo napromienianie od zewnątrz. Odczyny popromienne oceniono w oparciu o klasyfikacje EORTC/RTOG.WynikiŚredni czas obserwacji chorych wynosił 15 miesięcy – od 6 do 32 miesięcy. Tolerancja leczenia była dobra. Wszystkie chore otrzymały napromienianie zgodnie z planem. Wczesny odczyn popromienny w pochwie w stopniu G1 wystąpił u 8 chorych (7,3%). U 10 chorych (9%) poza obszarem leczenia, w okolicy podcewkowej wystąpiła odleżyna o powierzchni do 1,5 cm2, podobnie jak u chorych leczonych cylindrycznym aplikatorem Selektronowym, wynikająca z rozmiaru aplikatora i jego ucisku. Wczesne odczyny popromienne w pęcherzu i w odbytnicy o lekkim przebiegu wystąpiły odpowiednio u 5 i 4 chorych. Późna reakcja popromienna w pochwie w stopniu G2 wystąpiła u jednej chorej. Późna reakcja popromienna w pęcherzu i w odbytnicy w stopniu G1 wystąpiła odpowiednio u 2 i 4 chorych. U jednej chorej wystąpił odczyn popromienny w odbytnicy w stopniu G4. W okresie obserwacji loko-regionalne niepowodzenie wystąpiło u 3 chorych (2,7%).WnioskiBrachyterapia dopochwowa PDR wydaje się być obiecującą, bezpieczną i dobrze tolerowaną metodą uzupełniającego leczenia chorych operowanych z powodu raka trzonu macicy