Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy.

Leishmaniasis is a geographically widespread severe disease, with an increasing incidence of two million cases per year and 350 million people from 88 countries at risk. The causative agents are species of Leishmania, a protozoan flagellate. Visceral leishmaniasis, the most severe form of the disease, lethal if untreated, is caused by species of the Leishmania donovani complex. These species are morphologically indistinguishable but have been identified by molecular methods, predominantly multilocus enzyme electrophoresis. We have conducted a multifactorial genetic analysis that includes DNA sequences of protein-coding genes as well as noncoding segments, microsatellites, restriction-fragment length polymorphisms, and randomly amplified polymorphic DNAs, for a total of approximately 18,000 characters for each of 25 geographically representative strains. Genotype is strongly correlated with geographical (continental) origin, but not with current taxonomy or clinical outcome. We propose a new taxonomy, in which Leishmania infantum and L. donovani are the only recognized species of the L. donovani complex, and we present an evolutionary hypothesis for the origin and dispersal of the species. The genus Leishmania may have originated in South America, but diversified after migration into Asia. L. donovani and L. infantum diverged approximately 1 Mya, with further divergence of infraspecific genetic groups between 0.4 and 0.8 Mya. The prevailing mode of reproduction is clonal, but there is evidence of genetic exchange between strains, particularly in Africa

The model constraints from the observed trends for the quasi-periodic oscillation in RE J1034+396

We analyze the time variability of the X-ray emission of RE J1034+396 -- an active galactic nucleus with the first firm detection of a quasi-periodic oscillations (QPO). Based on the results of a wavelet analysis, we find a drift in the QPO central frequency. The change in the QPO frequency correlates with the change in the X-ray flux with a short time delay. The data specifically suggest a linear dependence between the QPO period and the flux, and this gives important constraints on the QPO models. In particular, it excludes explanation in terms of the orbiting hot spot model close to a black hole. Linear structures such as shocks, spiral waves, or very distant flares are favored.Comment: Astronomy & Astrophysics, in pres

Comparison of Catalysts in the Point of View of Pellet Stove Flue Gas Purification

Monolithic catalysts are used as a method for the flue gases purifying by oxidation of gas products from incomplete combustion. This study is focused on three different types of monolithic catalysts and quantification of their degree of influence on mass concentration of carbon monoxide (CO) and organic gaseous compounds (OGCs) in real small-scale wood pellet stove. Catalysts were placed right behind the stove at the flue gas outlet. The comparison consisted of quantification of their influence on the selected pollutants during the few-hours steady operation of the small-scale pellet stove. Reference values of the selected pollutants were defined during the combustion test without a catalyst installed. In this article, three catalysts based on different active compounds: WO3–V2O5, Pd and Pt were tested. The palladium-based catalyst has proven the best degree of conversion of CO (almost 78%). The platinum-based catalyst has proven the best degree of conversion of OGC (almost 64%). Due to a big degree of clogging by solid particles of all catalysts during the tests, it is impossible to operate the chosen stove with tested types of catalysts in normal operation at home conditions. Without any type of periodical cleaning (every few hours), there is a serious danger of leakage of the flue gas out of the stove. Further investigations should evaluate the degree of clogging in a long-term operation and should propose a method to avoid any danger of the flue gas leaking caused by the catalysts.This article was prepared within OP RDE, the project ‘Research on the identification of combustion of unsuitable fuels and systems of self-diagnostics of boilers combusting solid fuels for domestic heating’, identification code CZ.02.1.01/0.0/0.0/18_069/0010049, with the financial support from the European Regional Development Fund. This article was also prepared within the project SP2019/83 ‘Monitoring the operating parameters of a small combustion equipment and determining its effect on condensation of water in the flue gas’ and was elaborated in the framework of the grant program ‘Support for Science and Research in the Moravia-Silesia Region 2018’, (RRC/10/2018), financed from the budget of the Moravian-Silesian Region

Farsighted Risk Mitigation of Lateral Movement Using Dynamic Cognitive Honeypots

Lateral movement of advanced persistent threats has posed a severe security challenge. Due to the stealthy and persistent nature of the lateral movement, defenders need to consider time and spatial locations holistically to discover latent attack paths across a large time-scale and achieve long-term security for the target assets. In this work, we propose a time-expanded random network to model the stochastic service links in the user-host enterprise network and the adversarial lateral movement. We design cognitive honeypots at idle production nodes and disguise honey links as service links to detect and deter the adversarial lateral movement. The location of the honeypot changes randomly at different times and increases the honeypots' stealthiness. Since the defender does not know whether, when, and where the initial intrusion and the lateral movement occur, the honeypot policy aims to reduce the target assets' Long-Term Vulnerability (LTV) for proactive and persistent protection. We further characterize three tradeoffs, i.e., the probability of interference, the stealthiness level, and the roaming cost. To counter the curse of multiple attack paths, we propose an iterative algorithm and approximate the LTV with the union bound for computationally efficient deployment of cognitive honeypots. The results of the vulnerability analysis illustrate the bounds, trends, and a residue of LTV when the adversarial lateral movement has infinite duration. Besides honeypot policies, we obtain a critical threshold of compromisability to guide the design and modification of the current system parameters for a higher level of long-term security. We show that the target node can achieve zero vulnerability under infinite stages of lateral movement if the probability of movement deterrence is not less than the threshold

Beech Leaves Briquettes’ and Standard Briquettes’ Combustion: Comparison of Flue Gas Composition

Biomass stoves are not only popular, widespread and important sources of heat but are also not negligible sources of pollutants. The present study had two objectives in this field of research. The first one was to determine the difference between standard wooden and beech leaves briquettes flue gas composition during similar, standard home combustion conditions. The second objective was to determine the possibility of decreasing the mass concentration of pollutants contained in the flue gas produced by standard and alternative fuel combustion, i.e. wooden briquettes and beech leaves briquettes, by an oxidation catalyst. Significantly higher mass concentration of nitrogen oxides (NOx), almost 2.5 times higher, in the flue gas was observed during the beech leaves combustion. Both fuels reached the edge of actual legislation limit (European Standard Commission regulation [EU] 2015/1185) in case of mass concentration of carbon monoxide (CO). This issue was solved by a palladium-based catalyst with average degree of conversion around 82%. The catalyst also influences flue gas composition from mass concentration of propane point of view with average degree of conversion around 15%. The mass fraction of sulphur, occurring in the beech leaves briquettes, did not cause any issue to the catalyst in terms of its degree of CO conversion. Due to the test results from the beech leaves briquettes, i.e. high mass fraction of ash and high mass concentration of NOx in the flue gas, it is appropriate to use this kind of fuel as secondary fuel during the co-combustion process.This work was supported by the Doctoral grant competition VŠB TU-Ostrava, reg. no. CZ.0 2.2.69/0.0./0.0/19_073/0016945 within the Operational Programme Research, Development and Education, under project DGS/TEAM/2020-035 "Determination of oxidation catalysts characteristics during the flue gas purification"

Quasiperiodic oscillations in a strong gravitational field around neutron stars testing braneworld models

The strong gravitational field of neutron stars in the brany universe could be described by spherically symmetric solutions with a metric in the exterior to the brany stars being of the Reissner-Nordstrom type containing a brany tidal charge representing the tidal effect of the bulk spacetime onto the star structure. We investigate the role of the tidal charge in orbital models of high-frequency quasiperiodic oscillations (QPOs) observed in neutron star binary systems. We focus on the relativistic precession model. We give the radial profiles of frequencies of the Keplerian (vertical) and radial epicyclic oscillations. We show how the standard relativistic precession model modified by the tidal charge fits the observational data, giving estimates of the allowed values of the tidal charge and the brane tension based on the processes going in the vicinity of neutron stars. We compare the strong field regime restrictions with those given in the weak-field limit of solar system experiments.Comment: 26 pages, 6 figure

Anomalous Magnetoresistance by Breaking Ice Rule in Bi2Ir2O7/Dy2Ti2O7 Heterostructure

While geometrically frustrated quantum magnets are known for a variety of exotic spin states that are of great interests of understanding emergent phenomena as well as enabling revolutionary quantum technologies, most of them are necessarily good insulators which are difficult to be integrated with modern electrical circuit that relies on moving charge carriers. The grand challenge of converting fluctuations and excitations of frustrated moments into electronic responses is finding ways to introduce charge carriers that interact with the localized spins without destroying the spin states. Here, we show that, by designing a Bi2Ir2O7/Dy2Ti2O7 heterostructure, the breaking of the spin ice rule in insulating Dy2Ti2O7 can lead to a charge response in the Bi2Ir2O7 conducting layer that can be detected as anomalous magnetoresistance. These results demonstrate a novel and feasible interfacial approach for electronically probing exotic spin states in insulating magnets, laying out a blueprint for the metallization of frustrated quantum magnets

Near-infrared polarimetry setting constraints on the orbiting spot model for Sgr A* flares

Context: Recent near-infrared polarization measurements of SgrA* show that its emission is significantly polarized during flares and consists of a non- or weakly polarized main flare with highly polarized sub-flares. The flare activity suggests a quasi-periodicity of ~20 minutes in agreement with previous observations. Aims: By simultaneous fitting of the lightcurve fluctuations and the time-variable polarization angle, we address the question of whether these changes are consistent with a simple hot spot/ring model, in which the interplay of relativistic effects plays the major role, or whether some more complex dependency of the intrinsic emissivity is required. Methods: We discuss the significance of the 20min peak in the periodogram of a flare from 2003. We consider all general relativistic effects that imprint on the polarization degree and angle and fit the recent polarimetric data, assuming that the synchrotron mechanism is responsible for the intrinsic polarization and considering two different magnetic field configurations. Results: Within the quality of the available data, we think that the model of a single spot in addition to an underlying ring is favoured. In this model the broad near-infrared flares of Sgr A* are due to a sound wave that travels around the MBH once while the sub-flares, superimposed on the broad flare, are due to transiently heated and accelerated electrons which can be modeled as a plasma blob. Within this model it turns out that a strong statement about the spin parameter is difficult to achieve, while the inclination can be constrained to values > 35 deg on a 3sigma level.Comment: accepted by A&A for publicatio