Cell-specific modulation of monocarboxylate transporter expression contributes to the metabolic reprograming taking place following cerebral ischemia.

Monocarboxylate transporters (MCTs) are involved in lactate trafficking and utilization by brain cells. As lactate is not only overproduced during ischemia but its utilization was shown to be essential upon recovery, we analyzed the expression of the main cerebral MCTs at 1 and 24h after an ischemic insult induced by a transient occlusion of the left middle cerebral artery (MCAO) in CD1 mice (n=5, 7 and 10 for control, 1 and 24h groups, respectively). After 1h of reperfusion, an upregulation of the three MCTs was observed in the striatum (MCT1 ipsilateral 2.73 ± 0.2 and contralateral 2.01 ± 0.4; MCT2 ipsilateral 2.1 ± 0.1; MCT4 ipsilateral 1.65 ± 0.1) and in the surrounding cortex of both the ipsilateral (MCT1 2.4 ± 0.4; MCT2 1.62 ± 0.2; MCT4 1.31 ± 0.1) and contralateral (MCT1 2.78 ± 0.4; MCT2 1.76 ± 0.2) hemispheres, compared to the corresponding sham hemispheres. An increase of MCT1 (ipsilateral 2.1 ± 0.2) and MCT2 (contralateral 1.9 ± 0.1) expression was also observed in the hippocampus, while no effect was observed for MCT4. At 24h of reperfusion, total MCT2 and MCT4 expressions were decreased in the striatum (MCT2 ipsilateral 0.32 ± 0.1 and contralateral 0.63 ± 0.1; MCT4 ipsilateral 0.59 ± 0.1) and the surrounding cortex (MCT4 ipsilateral 0.67 ± 0.1), compared to the sham. At the cellular level, neurons which usually express only MCT2 strongly expressed MCT1 at both time points. Surprisingly, staining for MCT4 appeared on neurons and was strong at 24h post-insult, in the striatum and the cortex of both hemispheres. A similar expression pattern was observed also in the ipsilateral hemisphere of the sham operated animals at 24h. Overall, our study indicates that cell-specific changes in MCT expression induced by an ischemic insult may participate to the metabolic adaptations taking place in the brain after a transient ischemic episode

Accuracy analysis of vertical deflection data observed with the Hannover Digital Zenith Camera System TZK2-D

This paper analyses the accuracy of vertical deflection measurements carried out with the Digital Zenith Camera System TZK2-D, an astrogeodetic state-of-the-art instrumentation developed at the University of Hannover. During 107 nights over a period of 3.5 years, the system was used for repeated vertical deflection observations at a selected station in Hannover. The acquired data set consists of about 27,300 single measurements and covers 276 h of observation time, respectively. For the data collected at an earlier stage of development (2003 to 2004), the accuracy of the nightly mean values has been found to be about 0".10-0".12. Due to applying a refined observation strategy since 2005, the accuracy of the vertical deflection measurements was enhanced into the unprecedented range of 0".05-0".08. Accessing the accuracy level of 0".05 requires usually 1 h of observational data, while the 0".08 accuracy level is attained after 20 min measurement time. In comparison to the analogue era of geodetic astronomy, the accuracy of vertical deflection observations is significantly improved by about one order of magnitude

New directional archeomagnetic data of burned cave sediments from Switzerland and geomagnetic field variations in Central Europe

This paper presents new directional archeomagnetic data from nine Meso-/Neolithic fireplaces, sampled in a cave shelter, at Arconciel, in western Switzerland. Rock magnetic measurements indicate a homogenous magnetic mineralogy in all fireplaces, with magnetite as the main magnetic carrier. The remanent magnetization is stable and generally shows one characteristic directional component. Nine new directions, which were obtained from Arconciel, are combined with 356 other archeomagnetic data from a circular area with a radius of 700km around this site, to obtain a penalized least square spline fit for the past 9000yr. We found in general good agreement with other local compilations, such as the Balkan curve, the regional SCHA.DIF.8k model and with lake sediments from UK, Fennoscandia and Switzerland. Nevertheless, a time lag of several centuries is observed for a declination maximum between the archeomagnetic spline fit and the other European data records around 5900BC. This time lag is also observed in the Swiss lake sediment record; therefore we interpret this shift as a local feature of the Earth's magnetic fiel

A probable dual mode of action for both L- and D-lactate neuroprotection in cerebral ischemia.

Lactate has been shown to offer neuroprotection in several pathologic conditions. This beneficial effect has been attributed to its use as an alternative energy substrate. However, recent description of the expression of the HCA1 receptor for lactate in the central nervous system calls for reassessment of the mechanism by which lactate exerts its neuroprotective effects. Here, we show that HCA1 receptor expression is enhanced 24 hours after reperfusion in an middle cerebral artery occlusion stroke model, in the ischemic cortex. Interestingly, intravenous injection of L-lactate at reperfusion led to further enhancement of HCA1 receptor expression in the cortex and striatum. Using an in vitro oxygen-glucose deprivation model, we show that the HCA1 receptor agonist 3,5-dihydroxybenzoic acid reduces cell death. We also observed that D-lactate, a reputedly non-metabolizable substrate but partial HCA1 receptor agonist, also provided neuroprotection in both in vitro and in vivo ischemia models. Quite unexpectedly, we show D-lactate to be partly extracted and oxidized by the rodent brain. Finally, pyruvate offered neuroprotection in vitro whereas acetate was ineffective. Our data suggest that L- and D-lactate offer neuroprotection in ischemia most likely by acting as both an HCA1 receptor agonist for non-astrocytic (most likely neuronal) cells as well as an energy substrate

Expected accuracy of tilt measurements on a novel hexapod-based Digital zenith camera system: A Monte-Carlo simulation study

Digital zenith camera systems (DZCS) are dedicated astronomical-geodetic measurement systems for the observation of the direction of the plumb line. A DZCS key component is a pair of tilt meters for the determination of the instrumental tilt with respect to the plumb line. Highest accuracy (i.e., 0.1 arc-seconds or better) is achieved in practice through observation with precision tilt meters in opposite faces (180° instrumental rotation), and application of rigorous tilt reduction models. A novel concept proposes the development of a hexapod (Stewart platform)-based DZCS. However, hexapod-based total rotations are limited to about 30°–60° in azimuth (equivalent to ±15° to ±30° yaw rotation), which raises the question of the impact of the rotation angle between the two faces on the accuracy of the tilt measurement. The goal of the present study is the investigation of the expected accuracy of tilt measurements to be carried out on future hexapod-based DZCS, with special focus placed on the role of the limited rotation angle. A Monte-Carlo simulation study is carried out in order to derive accuracy estimates for the tilt determination as a function of several input parameters, and the results are validated against analytical error propagation.As the main result of the study, limitation of the instrumental rotation to 60° (30°) deteriorates the tilt accuracy by a factor of about 2 (4) compared to a 180° rotation between the faces. Nonetheless, a tilt accuracy at the 0.1 arc-second level is expected when the rotation is at least 45°, and 0.05 arc-second (about 0.25 microradian) accurate tilt meters are deployed. As such, a hexapod-based DZCS can be expected to allow sufficiently accurate determination of the instrumental tilt. This provides supporting evidence for the feasibility of such a novel instrumentation. The outcomes of our study are not only relevant to the field of DZCS, but also to all other types of instruments where the instrumental tilt must be corrected. Examples include electronic theodolites or total stations, gravity meters, and other hexapod-based telescopes

Study of the Earth’s short-scale gravity field using the ERTM2160 gravity model

This paper describes the computation and analysis of the Earth’s short-scale gravity field through high-resolution gravity forward modelling using the Shuttle Radar Topography Mission (SRTM) global topography model. We use the established residual terrain modelling technique along with advanced computational resources and massive parallelisation to convert the high-pass filtered SRTM topography – complemented with bathymetric information in coastal zones – to implied short-scale gravity effects. The result is the ERTM2160 model (Earth Residual Terrain Modelled-gravity field with the spatial scales equivalent to spherical-harmonic coefficients up to degree 2160 removed). ERTM2160, used successfully for the construction of the GGMplus gravity maps, approximates the short-scale (i.e., ~10 km down to ~250 m) gravity field in terms of gravity disturbances, quasi/geoid heights and vertical deflections at ~3 billion gridded points within ±60 latitude. ERTM2160 reaches maximum values for the quasi/geoid height of ~30 cm, gravity disturbance in excess of 100 mGal, and vertical deflections of ~30 arc-seconds over the Himalaya mountains.Analysis of the ERTM2160 field as a function of terrain roughness shows in good approximation a linear relationship between terrain roughness and gravity effects, with values of ~1.7 cm (quasi/geoid heights), ~11 mGal (gravity disturbances) and 1.5 arc-seconds (vertical deflections) signal strength per 100 m standard deviation of the terrain. These statistics can be used to assess the magnitude of omitted gravity signals over various types of terrain when using degree-2160 gravity models such as EGM2008. Applications for ERTM2160 are outlined including its use in gravity smoothing procedures, augmentation of EGM2008, fill-in for future ultra-high resolution gravity models in spherical harmonics, or calculation of localised or global power spectra of Earth’s short-scale gravity field. ERTM2160 is freely available via http://ddfe.curtin.edu.au/gravitymodels/ERTM2160

Boundary Layer Flow Control by an Array of Ramp-Shaped Vortex Generators

Flow field survey results for the effect of ramp-shaped vortex generators (VG) on a turbulent boundary layer are presented. The experiments are carried out in a low-speed wind tunnel and the data are acquired primarily by hot-wire anemometry. Distributions of mean velocity and turbulent stresses as well as streamwise vorticity, on cross-sectional planes at various downstream locations, are obtained. These detailed flow field properties, including the boundary layer characteristics, are documented with the primary objective of aiding possible computational investigations. The results show that VG orientation with apex upstream, that produces a downwash directly behind it, yields a stronger pair of streamwise vortices. This is in contrast to the case with apex downstream that produces a pair of vortices of opposite sense. Thus, an array of VG s with the former orientation, usually considered for film-cooling application, may also be superior for mixing enhancement and boundary layer separation control. (See CASI ID 20120009374 for Supplemental CD-ROM.

Indirect evaluation of Mars Gravity Model 2011 using a replication experiment on Earth

Curtin University’s Mars Gravity Model 2011 (MGM2011) is a high-resolution composite set of gravity field functionals that uses topography-implied gravity effects at medium- and short-scales (~125 km to ~3 km) to augment the space-collected MRO110B2 gravity model. Ground-truth gravity observations that could be used for direct validation of MGM2011 are not available on Mars’s surface. To indirectly evaluate MGM2011 and its modelling principles, an as-close-as-possible replication of the MGM2011 modelling approach was performed on Earth as the planetary body with most detailed gravity field knowledge available. Comparisons among six ground-truth data sets (gravity disturbances, quasigeoid undulations and vertical deflections) and the MGM2011-replication over Europe and North America show unanimously that topography-implied gravity information improves upon space-collected gravity models over areas with rugged terrain. The improvements are ~55% and ~67% for gravity disturbances, ~12% and ~47% for quasigeoid undulations, and ~30% to ~50% for vertical deflections. Given that the correlation between space-collected gravity and topography is higher for Mars than Earth at spatial scales of a few 100 km, topography-implied gravity effects are more dominant on Mars. It is therefore reasonable to infer that the MGM2011 modelling approach is suitable, offering an improvement over space-collected Martian gravity field models

Оцінка газоносності метановугільних родовищ. Методичні рекомендаціїдо виконання практичних робітдля студентів спеціальності 185«Нафтогазова інженерія та технології»/

Розглянуто теоретичні відомості про ключові питання розрахунків параметрів зон скупчення метану у порушеному і непорушеному вуглепородних масивах. Рекомендації будуть сприяти активізації знань студентів. Призначено для бакалаврів спеціальності 185 «Нафтогазова інженерія та технології»