Lower bounds for moments of zeta and LL-functions revisited

This paper describes a method to compute lower bounds for moments of $\zeta$ and $L$-functions. The method is illustrated in the case of moments of $|\zeta(\frac 12+it)|$, where the results are new for small moments $0< k<1$

Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.

Background: Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species that produce butanol are, however, unable to grow on crystalline cellulose. In contrast, those saccharolytic species that produce predominantly ethanol, such as Clostridium thermocellum and Clostridium cellulolyticum, degrade crystalline cellulose with high efficiency due to their possession of a multienzyme complex termed the cellulosome. This has led to studies directed at endowing butanol-producing species with the genetic potential to produce a cellulosome, albeit by localising the necessary transgenes to unstable autonomous plasmids. Here we have explored the potential of our previously described Allele-Coupled Exchange (ACE) technology for creating strains of the butanol producing species Clostridium acetobutylicum in which the genes encoding the various cellulosome components are stably integrated into the genome. Results: We used BioBrick2 (BB2) standardised parts to assemble a range of synthetic genes encoding C. thermocellum cellulosomal scaffoldin proteins (CipA variants) and glycoside hydrolases (GHs, Cel8A, Cel9B, Cel48S and Cel9K) as well as synthetic cellulosomal operons that direct the synthesis of Cel8A, Cel9B and a truncated form of CipA. All synthetic genes and operons were integrated into the C. acetobutylicum genome using the recently developed ACE technology. Heterologous protein expression levels and mini-cellulosome self-assembly were assayed by western blot and native PAGE analysis. Conclusions: We demonstrate the successful expression, secretion and self-assembly of cellulosomal subunits by the recombinant C. acetobutylicum strains, providing a platform for the construction of novel cellulosomes. © 2013 Kovács et al.; licensee BioMed Central Ltd

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we explore novel DNA integration strategies that do not employ them, and instead exploit (i) activation or inactivation of genes leading to a selectable phenotype, and (ii) asymmetrical regions of homology to control the order of recombination events. We focus here on the industrial biofuel-producing bacterium Clostridium acetobutylicum, which previously lacked robust integration tools, but the approach we have developed is broadly applicable. Large sequences can be delivered in a series of steps, as we demonstrate by inserting the chromosome of phage lambda (minus a region apparently unstable in Escherichia coli in our cloning context) into the chromosome of C. acetobutylicum in three steps. This work should open the way to reliable integration of DNA including large synthetic constructs in diverse microorganisms. © 2011 The Author(s)

Sharp Upper Bounds for Fractional Moments of the Riemann Zeta Function

We establish sharp upper bounds for the 2kth moment of the Riemann zeta function on the critical line, for all real 0 ⩽ k ⩽ 2⁠. This improves on earlier work of Ramachandra, Heath-Brown and Bettin–Chandee–Radziwiłł

IUE archived spectra

The International Ultraviolet Explorer (IUE) Satellite has been in continuous operation since January 26, 1978. To date, approximately 65,000 spectra have been stored in an archive at Goddard Space Flight Center in Greenbelt, MD. A number of procedures have been generated to facilitate access to the data in the IUE spectral archive. This document describes the procedures which include on-line quick look of the displays, search of an observation data base for selected observations, and several methods for ordering data from the archive

Galaxy Morphology from NICMOS Parallel Imaging

We present high resolution NICMOS images of random fields obtained in parallel to other HST observations. We present galaxy number counts reaching H=24. The H-band galaxy counts show good agreement with the deepest I- and K-band counts obtained from ground-based data. We present the distribution of galaxies with morphological type to H<23. We find relatively fewer irregular galaxies compared to an I-band sample from the Hubble Deep Field, which we attribute to their blue color, rather than to morphological K-corrections. We conclude that the irregulars are intrinsically faint blue galaxies at z<1.Comment: 13 pages, including 4 figures. Accepted for publication in ApJ Letter

Low surface gravitational acceleration of Mars results in a thick and weak lithosphere : implications for topography, volcanism, and hydrology

The first author acknowledges funding from an Initiative d’Excellence (IDEX) “Attractivité” grant (VOLPERM), funded by the University of Strasbourg. M.H. also acknowledges support from the CNRS (INSU 2016-TelluS-ALEAS).Surface gravitational acceleration (surface gravity) on Mars, the second-smallest planet in the Solar System, is much lower than that on Earth. A direct consequence of this low surface gravity is that lithostatic pressure is lower on Mars than on Earth at any given depth. Collated published data from deformation experiments on basalts suggest that, throughout its geological history (and thus thermal evolution), the Martian brittle lithosphere was much thicker but weaker than that of present-day Earth as a function solely of surface gravity. We also demonstrate, again as a consequence of its lower surface gravity, that the Martian lithosphere is more porous, that fractures on Mars remain open to greater depths and are wider at a given depth, and that the maximum penetration depth for opening-mode fractures (i.e., joints) is much deeper on Mars than on Earth. The result of a weak Martian lithosphere is that dykes—the primary mechanism for magma transport on both planets—can propagate more easily and can be much wider on Mars than on Earth. We suggest that this increased the efficiency of magma delivery to and towards the Martian surface during its volcanically active past, and therefore assisted the exogeneous and endogenous growth of the planet's enormous volcanoes (the heights of which are supported by the thick Martian lithosphere) as well as extensive flood-mode volcanism. The porous and pervasively fractured (and permeable) nature of the Martian lithosphere will have also greatly assisted the subsurface storage of and transport of fluids through the lithosphere throughout its geologically history. And so it is that surface gravity, influenced by the mass of a planetary body, can greatly modify the mechanical and hydraulic behaviour of its lithosphere with manifest differences in surface topography and geomorphology, volcanic character, and hydrology.PostprintPeer reviewe

Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo.

Spores of some species of the strictly anaerobic bacteria Clostridium naturally target and partially lyse the hypoxic cores of tumors, which tend to be refractory to conventional therapies. The anti-tumor effect can be augmented by engineering strains to convert a non-toxic prodrug into a cytotoxic drug specifically at the tumor site by expressing a prodrug-converting enzyme (PCE). Safe doses of the favored prodrug CB1954 lead to peak concentrations of 6.3 μM in patient sera, but at these concentration(s) known nitroreductase (NTR) PCEs for this prodrug show low activity. Furthermore, efficacious and safe Clostridium strains that stably express a PCE have not been reported. Here we identify a novel nitroreductase from Neisseria meningitidis, NmeNTR, which is able to activate CB1954 at clinically-achievable serum concentrations. An NmeNTR expression cassette, which does not contain an antibiotic resistance marker, was stably localized to the chromosome of Clostridium sporogenes using a new integration method, and the strain was disabled for safety and containment by making it a uracil auxotroph. The efficacy of Clostridium-Directed Enzyme Prodrug Therapy (CDEPT) using this system was demonstrated in a mouse xenograft model of human colon carcinoma. Substantial tumor suppression was achieved, and several animals were cured. These encouraging data suggest that the novel enzyme and strain engineering approach represent a promising platform for the clinical development of CDEPT