Life on the Borderline: Reconceptualising the Experiences of People With a Diagnosis of Borderline Personality Disorder

The label of BPD was introduced as a formal psychiatric diagnosis in 1952. The reliability and validity of the diagnosis has been a source of contention in the professional field since this time. The diagnostic criteria have been frequently revised with a view to improve the reliability and validity of the construct. This research aimed to explore the stories of people with a diagnosis of BPD and to assess whether there were commonly shared experiences between them. Semi-structured interviews were conducted with eight people who had been given a diagnosis of BPD. Participants were recruited from a range of internet sources, twitter, forums and support networks. A Grounded Theory analysis of the data was conducted, and three categories were generated; Intense Experiences of Emotion, The Importance of Understanding, and the Fear of Repeated Relational Patterns. A conceptual model was not generated from the data. This was concluded to be reflective of the issues of validity and heterogeneity in the clinical population. The findings are discussed in relation to existing literature. Clinical implications are made which include paying attention to the individual stories told by people with a diagnosis of BPD, understanding the complex and unique function of self-harm and responding to aetiology rather than symptoms. Finally, recommendations for future research are highlighted

Plasma enhanced chemical vapor deposition of SiO_2 using novel alkoxysilane precursors

This communication describes our results using these novel alkoxysilane precursors for PECVD of SiO_2 films in an inductively coupled rf plasma reactor. The effects of deposition time, rf power, and organosilane pressure on the films’ characteristics are described

A modified molecular beam instrument for the imaging of radicals interacting with surfaces during plasma processing

A new instrument employing molecular beam techniques and laser induced fluorescence(LIF) for measuring the reactivity of gas phase radicals at the surface of a depositing film has been designed and characterized. The instrument uses an inductively coupled plasma source to create a molecular beam containing essentially all plasma species. A tunable excimer pumped dye laser is used to excite a single species in this complex molecular beam.LIF signals are imaged onto a gated, intensified charge coupled device (ICCD) to provide spatial resolution. ICCD images depict the fluorescence from molecules both in the molecular beam and scattering from the surface of a depositing film. Data collected with and without a substrate in the path of the molecular beam provide information about the surface reactivity of the species of interest. Here, we report the first measurements using the third generation imaging of radicals interacting with surfaces apparatus. We have measured the surface reactivity of SiH molecules formed in a 100% SiH_4 plasma during deposition of an amorphous hydrogenated silicon film. On a 300 K Si (100) substrate, the reactivity of SiH is near unity. The substrate temperature dependence (300–673 K) of the reactivity is also reported. In addition, reactivity measurements for OH molecules formed in a water plasma are presented. In contrast to the SiH molecule, the reactivity of OH radicals is 0.55±0.05 on the surface of a Si (100) substrate

Periodicities in sunspot activity during solar cycle 23

The data of sunspot numbers, sunspot areas and solar flare index during cycle 23 are analyzed to investigate the intermediate-term periodicities. Power spectral analysis has been performed separately for the data of the whole disk, northern and southern hemispheres of the Sun. Several significant midrange periodicities ($\sim$175, 133, 113, 104, 84, 63 days) are detected in sunspot activity. Most of the periodicities in sunspot numbers generally agree with those of sunspot areas during the solar cycle 23. The study reveals that the periodic variations in the northern and southern hemispheres of the Sun show a kind of asymmetrical behavior. Periodicities of $\sim$175 days and $\sim$133 days are highly significant in the sunspot data of northern hemisphere showing consistency with the findings of Lean (1990) during solar cycles 12-21. On the other hand, southern hemisphere shows a strong periodicity of about 85 days in terms of sunspot activity. The analysis of solar flare index data of the same time interval does not show any significant peak. The different periodic behavior of sunspot and flare activity can be understood in the light of hypothesis proposed by Ballester et al. (2002), which suggests that during cycle 23, the periodic emergence of magnetic flux partly takes place away from developed sunspot groups and hence may not necessarily increase the magnetic complexity of sunspot groups that leads to the generation of flares.Comment: 4 pages, 4 figure

Dipolar-coupled moment correlations in clusters of magnetic nanoparticles

Here, we investigate the nature of the moment coupling between 10-nm DMSA-coated magnetic nanoparticles, in both colloidal dispersion and in powder form. The individual iron oxide cores were composed of > 95% maghemite and agglomerated to clusters. At room temperature the ensemble behaved as a superparamagnet according to M\"ossbauer and magnetization measurements, however, with clear signs of dipolar interactions at low temperatures. Analysis of temperature-dependent AC susceptibility data in the superparamagnetic regime indicates a tendency for dipolar coupled anticorrelations of the core moments within the clusters. To resolve the directional correlations between the particle moments we performed polarized small-angle neutron scattering and determined the magnetic spin-flip cross-section of the powder in low magnetic field at 300 K. We extract the underlying pair distance distribution function of the magnetization vector field by an indirect Fourier transform of the cross-section, and which suggests positive as well as negative correlations between nearest neighbor moments, with anticorrelations clearly dominating for next-nearest moments. These tendencies are confirmed by Monte Carlo simulations of such core-clusters.Comment: 11 pages, 6 figure

Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

Tracking stem cells in vivo using non-invasive techniques is critical to evaluate the efficacy and safety of stem cell therapies. Superparamagnetic iron oxide nanoparticles (SPIONs) enable cells to be tracked using magnetic resonance imaging (MRI), but to obtain detectable signal cells need to be labelled with a sufficient amount of iron oxide. For the majority of SPIONs, this can only be obtained with the use of transfection agents, which can adversely affect cell health. Here, we have synthesised a library of dextran-based polymer coated SPIONs with varying surface charge from −1.5 mV to +18.2 mV via a co-precipitation approach and investigated their ability to be directly internalised by stem cells without the need for transfection agents. The SPIONs were colloidally stable in physiological solutions. The crystalline phase of the particles was confirmed with powder X-ray diffraction and their magnetic properties were characterised using SQUID magnetometry and magnetic resonance. Increased surface charge led to six-fold increase in uptake of particles into stem cells and higher MRI contrast, with negligible change in cell viability. Cell tracking velocimetry was shown to be a more accurate method for predicting MRI contrast of stem cells compared to measuring iron oxide uptake through conventional bulk iron quantification

Recurrent Clostridium difficile infection associates with distinct bile acid and microbiome profiles

Background: The healthy microbiome protects against the development of Clostridium difficile infection (CDI), which typically develops following antibiotics. The microbiome metabolises primary to secondary bile acids, a process if disrupted by antibiotics, may be critical for the initiation of CDI. Aim: To assess the levels of primary and secondary bile acids associated with CDI and associated microbial changes. Methods: Stool and serum were collected from patients with (i) first CDI (fCDI), (ii) recurrent CDI (rCDI) and (iii) healthy controls. 16S rRNA sequencing and bile salt metabolomics were performed. Random forest regression models were constructed to predict disease status. PICRUSt analyses were used to test for associations between predicted bacterial bile salt hydrolase (BSH) gene abundances and bile acid levels. Results: Sixty patients (20 fCDI, 19 rCDI and 21 controls) were enrolled. Secondary bile acids in stool were significantly elevated in controls compared to rCDI and fCDI (P < 0.0001 and P = 0.0007 respectively). Primary bile acids in stool were significantly elevated in rCDI compared to controls (P < 0.0001) and in rCDI compared to fCDI (P = 0.02). Using random forest regression, we distinguished rCDI and fCDI patients 84.2% of the time using bile acid ratios. Stool deoxycholate to glycoursodeoxycholate ratio was the single best predictor. PICRUSt analyses found significant differences in predicted abundances of bacterial BSH genes in stool samples across the groups. Conclusions: Primary and secondary bile acid composition in stool was different in those with rCDI, fCDI and controls. The ratio of stool deoxycholate to glycoursodeoxycholate was the single best predictor of disease state and may be a potential biomarker for recurrence.American College of Gastroenterology (Clinical Research Award ACGJR-017-2015