Notes on Reproduction in the Brownback Salamander (Eurycea aquatica)

Life history data often are lacking for species with an uncertain taxonomic status. As phylogenetic analyses improve and our understanding of species boundaries become more refined, ecological information should likewise keep pace. Here, we report information on the timing of reproduction, clutch size, and nest attendance for the Brownback Salamander (Eurycea aquatica), a species recently documented to be a separate lineage after a long, controversial systematic history. We confirm that this species has the largest clutch size of any lineage within the Eurycea bislineata complex, and suggest that male and male-female nest attendance is not uncommon in this species. We suggest that, in addition to the genetic divergence documented between E. aquatica and other members of the E. bislineata complex, ecological differences also are present and possibly are influenced by the unique springs inhabited by Brownback Salamanders

Notes on Reproduction in the Brownback Salamander (Eurycea aquatica)

Life history data often are lacking for species with an uncertain taxonomic status. As phylogenetic analyses improve and our understanding of species boundaries become more refined, ecological information should likewise keep pace. Here, we report information on the timing of reproduction, clutch size, and nest attendance for the Brownback Salamander (Eurycea aquatica), a species recently documented to be a separate lineage after a long, controversial systematic history. We confirm that this species has the largest clutch size of any lineage within the Eurycea bislineata complex, and suggest that male and male-female nest attendance is not uncommon in this species. We suggest that, in addition to the genetic divergence documented between E. aquatica and other members of the E. bislineata complex, ecological differences also are present and possibly are influenced by the unique springs inhabited by Brownback Salamanders

The Outstanding Decisions of the United States Supreme Court in 1954

We perform a kinematic and morphological analysis of 44 star-forming galaxies at z ̃ 2 in the COSMOS legacy field using near-infrared spectroscopy from Keck/MOSFIRE and F160W imaging from CANDELS/3D-HST as part of the ZFIRE survey. Our sample consists of cluster and field galaxies from 2.0 < z < 2.5 with K-band multi-object slit spectroscopic measurements of their Hα emission lines. Hα rotational velocities and gas velocity dispersions are measured using the Heidelberg Emission Line Algorithm (HELA), which compares directly to simulated 3D data cubes. Using a suite of simulated emission lines, we determine that HELA reliably recovers input S 0.5 and angular momentum at small offsets, but V 2.2/σ g values are offset and highly scattered. We examine the role of regular and irregular morphology in the stellar mass kinematic scaling relations, deriving the kinematic measurement S 0.5, and finding {log}({S}0.5)=(0.38+/- 0.07){log}(M/{M}☉ -10)+(2.04+/- 0.03) with no significant offset between morphological populations and similar levels of scatter (̃0.16 dex). Additionally, we identify a correlation between M ⋆ and V 2.2/σ g for the total sample, showing an increasing level of rotation dominance with increasing M ⋆, and a high level of scatter for both regular and irregular galaxies. We estimate the specific angular momenta (j disk) of these galaxies and find a slope of 0.36 ± 0.12, shallower than predicted without mass-dependent disk growth, but this result is possibly due to measurement uncertainty at M ⋆ < 9.5 However, through a Kolmogorov-Smirnov test we find irregular galaxies to have marginally higher j disk values than regular galaxies, and high scatter at low masses in both populations

Predicting posttraumatic stress disorder after childbirth

Objective: around 50% of women report symptoms that indicate some aspect of their childbirth experience was 'traumatic', and at least 3.1% meet diagnosis for PTSD six months post partum. Here we aimed to conduct a prospective longitudinal study and examine predictors of birth-related trauma - predictors that included a range of pre-event factors - as a first step in the creation of a screening questionnaire. Method: of the 933 women who completed an assessment in their third trimester, 866 were followed-up at four to six week post partum. Two canonical discriminant function analyses were conducted to ascertain factors associated with experiencing birth as traumatic and, of the women who found the birth traumatic, which factors were associated with those who developed PTSD. Findings: a mix of 16 pre-birth predictor variables and event-specific predictor variables distinguished women who reported symptoms consistent with trauma from those who did not. Fourteen predictor variables distinguished women who went on to develop PTSD from those who did not. Conclusions: anxiety sensitivity to possible birthing problems, breached birthing expectations, and severity of any actual birth problem, predicted those who found the birth traumatic. Prior trauma was the single most important predictive factor of PTSD. Evaluating the utility of brief, cost-effective, and accurate screening for women at risk of developing birth-related PTSD is suggested

ZFOURGE: Extreme 5007A˚\AA emission may be a common early-lifetime phase for star-forming galaxies at z>2.5z > 2.5

Using the \prospector\ spectral energy distribution (SED) fitting code, we analyze the properties of 19 Extreme Emission Line Galaxies (EELGs) identified in the bluest composite SED in the \zfourge\ survey at $2.5 \leq z \leq 4$. \prospector\ includes a physical model for nebular emission and returns probability distributions for stellar mass, stellar metallicity, dust attenuation, and nonparametric star formation history (SFH). The EELGs show evidence for a starburst in the most recent 50 Myr, with the median EELG having a specific star formation rate (sSFR) of 4.6 Gyr$^{-1}$ and forming 15\% of its mass in this short time. For a sample of more typical star-forming galaxies (SFGs) at the same redshifts, the median SFG has a sSFR of 1.1 Gyr$^{-1}$ and forms only $4\%$ of its mass in the last 50 Myr. We find that virtually all of our EELGs have rising SFHs, while most of our SFGs do not. From our analysis, we hypothesize that many, if not most, star-forming galaxies at $z \geq 2.5$ undergo an extreme H$\beta$+[\hbox{{\rm O}\kern 0.1em{\sc iii}}] emission line phase early in their lifetimes. In a companion paper, we obtain spectroscopic confirmation of the EELGs as part of our {\sc MOSEL} survey. In the future, explorations of uncertainties in modeling the UV slope for galaxies at $z>2$ are needed to better constrain their properties, e.g. stellar metallicities.Comment: 11 pages, 5 figures (main figure is fig 5), accepted for publication in Ap

Development of a rapid, antimicrobial susceptibility test for E. coli based on low-cost, screen-printed electrodes

Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, Escherichia coli (E. coli) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the E. coli growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (p < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of E. coli within clinically relevant concentration ranges for conditions such as urinary tract infections

Airway epithelial specific deletion of Jun-N-terminal kinase 1 attenuates pulmonary fibrosis in two independent mouse models

© 2020 van der Velden et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The stress-induced kinase, c-Jun-N-terminal kinase 1 (JNK1) has previously been implicated in the pathogenesis of lung fibrosis. However, the exact cell type(s) wherein JNK1 exerts its pro-fibrotic role(s) remained enigmatic. Herein we demonstrate prominent activation of JNK in bronchial epithelia using the mouse models of bleomycin- or AdTGFβ1-induced fibrosis. Furthermore, in lung tissues of patients with idiopathic pulmonary fibrosis (IPF), active JNK was observed in various regions including type I and type II pneumocytes and fibroblasts. No JNK activity was observed in adjacent normal tissue or in normal control tissue. To address the role of epithelial JNK1, we ablated Jnk1 form bronchiolar and alveolar type II epithelial cells using CCSP-directed Cre recombinase-mediated ablation of LoxP-flanked Jnk1 alleles. Our results demonstrate that ablation of Jnk1 from airway epithelia resulted in a strong protection from bleomycin- or adenovirus expressing active transforming growth factor beta-1 (AdTGFβ1)-induced fibrosis. Ablation of the Jnk1 allele at a time when collagen increases were already present showed a reversal of existing increases in collagen content. Epithelial Jnk1 ablation resulted in attenuation of mesenchymal genes and proteins in lung tissue and preserved expression of epithelial genes. Collectively, these data suggest that epithelial JNK1 contributes to the pathogenesis of pulmonary fibrosis. Given the presence of active JNK in lungs from patients with IPF, targeting JNK1 in airway epithelia may represent a potential treatment strategy to combat this devastating disease

A novel live-attenuated vaccine candidate for mayaro Fever.

Mayaro virus (MAYV) is an emerging, mosquito-borne alphavirus that causes a dengue-like illness in many regions of South America, and which has the potential to urbanize. Because no specific treatment or vaccine is available for MAYV infection, we capitalized on an IRES-based approach to develop a live-attenuated MAYV vaccine candidate. Testing in infant, immunocompetent as well as interferon receptor-deficient mice demonstrated a high degree of attenuation, strong induction of neutralizing antibodies, and efficacy against lethal challenge. This vaccine strain was also unable to infect mosquito cells, a major safety feature for a live vaccine derived from a mosquito-borne virus. Further preclinical development of this vaccine candidate is warranted to protect against this important emerging disease

Towards a grapho-phonologically parsed corpus of medieval Scots:Database design and technical solutions

This paper presents a newly constructed corpus of sound-to-spelling mappings in medieval Scots, which stems from the work of the From Inglis to Scots (FITS) project. We have developed a systematic approach to the relationships between individual spellings and proposed sound values, and recorded these mutual links in a relational database. In this paper, we introduce the theoretical underpinnings of sound-to-spelling and spelling-to-sound mappings, and show how a Scots root morpheme undergoes grapho-phonological parsing, the analytical procedure that is employed to break down spelling sequences into sound units. We explain the data collection and annotation for the FITS Corpus (Alcorn et al., forthcoming), drawing attention to the extensive meta-data which accompany each analysed unit of spelling and sound. The database records grammatical and lexical information about the root, the positional arrangement of segments within the root, labels for the nuclei, vowels and consonants, the morphological context, and extra-linguistic detail of the text a given root was taken from (date, place and text type). With this wealth of information, the FITS corpus is capable of answering complex queries about the sound and spelling systems of medieval Scots. We also suggest how our methodology can be transferred to other non-standardised spelling systems