Hubble Space Telescope Observations of Comet 9P/Tempel 1 during the Deep Impact Encounter

We report on the Hubble Space Telescope program to observe periodic comet 9P/Tempel 1 in conjunction with NASA's Deep Impact mission. Our objectives were to study the generation and evolution of the coma resulting from the impact and to obtain wide-band images of the visual outburst generated by the impact. Two observing campaigns utilizing a total of 17 HST orbits were carried out: the first occurred on 2005 June 13-14 and fortuitously recorded the appearance of a new, short-lived fan in the sunward direction on June 14. The principal campaign began two days before impact and was followed by contiguous orbits through impact plus several hours and then snapshots one, seven, and twelve days later. All of the observations were made using the Advanced Camera for Surveys (ACS). For imaging, the ACS High Resolution Channel (HRC) provides a spatial resolution of 36 km (16 km/pixel) at the comet at the time of impact. Baseline images of the comet, made prior to impact, photometrically resolved the comet's nucleus. The derived diameter, 6.1 km, is in excellent agreement with the 6.0 +/- 0.2 km diameter derived from the spacecraft imagers. Following the impact, the HRC images illustrate the temporal and spatial evolution of the ejecta cloud and allow for a determination of its expansion velocity distribution. One day after impact the ejecta cloud had passed out of the field-of-view of the HRC.Comment: 15 pages, 14 postscript figures. Accepted for publication in Icarus special issue on Deep Impac

Percutaneous Anorectoplasty (PARP)-An Adaptable, Minimal-Invasive Technique for Anorectal Malformation Repair

Background: Anorectal malformations comprise a broad spectrum of disease. We developed a percutaneous anorectoplasty (PARP) technique as a minimal-invasive option for repair of amenable types of lesions. Methods: Patients who underwent PARP at five institutions from 2008 through 2021 were retrospectively analyzed. Demographic information, details of the operative procedure, and perioperative complications and outcomes were collected. Results: A total of 10 patients underwent the PARP procedure during the study interval. Patients either had low perineal malformations or no appreciable fistula. Most procedures were guided by ultrasound, fluoroscopy, or endoscopy. Median age at PARP was 3 days (range 1 to 311) days;eight patients were male. Only one intraoperative complication occurred, prompting conversion to posterior sagittal anorectoplasty. Functional outcomes in most children were highly satisfactory in terms of continence and functionality. Conclusions: The PARP technique is an excellent minimal-invasive alternative for boys born with perineal fistulae, as well as patients of both sexes without fistulae. The optimal type of guidance (ultrasound, fluoroscopy, or endoscopy) depends on the anatomy of the lesion and the presence of a colostomy at the time of repair

The ESA Hera mission to the binary asteroid (65803) Didymos: Planetary defense and science

The Hera mission is in development for launch in 2024 within the ESA Space Safety Program. Hera will contribute to the first deflection test of an asteroid, in the framework of the international NASA and ESA-supported Asteroid Impact and Deflection Assessment (AIDA) collaboration. Hera will also offer a great science return

The ESA Hera Mission: Investigating binary asteroid (65803) Didymos and the DART crater

On 26 September 2022 NASA's Double Asteroid Redirection Test (DART) spacecraft will impact Dimorphos, the satellite of asteroid (65803) Didymos. The impact will change Dimorphos' orbital period around Didymos. As Didymos is an eclipsing binary, and on a close flyby of Earth on this date, the period change can be detected by Earth-based observers. Before impact, DART will deploy the Light Italian Cubesat for Imaging of Asteroid (LICIACube) that will provide images of the first instants after impact. ESA’s Hera spacecraft will rendezvous with Didymos four years after the impact. It will perform the measurements necessary to fully understand the effect of the DART impact on Dimorphos, in particular by measuring its mass, and investigating its internal structure, and thus determining the momentum transfer and detailed characterization of the crater left by DART

The ESA Hera Mission to the near-earth asteroid binary (65803) Didymos: documentation of the NASA Dart Impact and full characterization of the asteroid system

The Hera mission is in development within the ESA Space Safety Program for launch in October 2024 with a Falcon 9 rocket. It will perform a rendezvous with the binary asteroid (65803) Didymos in early 2027 and investigate it over a period of 6 months [1]. Together with the NASA DART mission [2], Hera will contribute to the first deflection test of an asteroid, in the framework of the international NASA and ESA-supported Asteroid Impact and Deflection Assessment (AIDA) collaboration

Genomic basis of a social polymorphism in a halictid bee

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Genomic and transcriptomic changes complement each other in the pathogenesis of sporadic Burkitt lymphoma

Burkitt lymphoma (BL) is the most common B-cell lymphoma in children. Within the International Cancer Genome Consortium (ICGC), we performed whole genome and transcriptome sequencing of 39 sporadic BL. Here, we unravel interaction of structural, mutational, and transcriptional changes, which contribute to MYC oncogene dysregulation together with the pathognomonic IG-MYC translocation. Moreover, by mapping IGH translocation breakpoints, we provide evidence that the precursor of at least a subset of BL is a B-cell poised to express IGHA. We describe the landscape of mutations, structural variants, and mutational processes, and identified a series of driver genes in the pathogenesis of BL, which can be targeted by various mechanisms, including IG-non MYC translocations, germline and somatic mutations, fusion transcripts, and alternative splicing

The genomic and transcriptional landscape of primary central nervous system lymphoma

Primary lymphomas of the central nervous system (PCNSL) are mainly diffuse large B-cell lymphomas (DLBCLs) confined to the central nervous system (CNS). Molecular drivers of PCNSL have not been fully elucidated. Here, we profile and compare the whole-genome and transcriptome landscape of 51 CNS lymphomas (CNSL) to 39 follicular lymphoma and 36 DLBCL cases outside the CNS. We find recurrent mutations in JAK-STAT, NFkB, and B-cell receptor signaling pathways, including hallmark mutations in MYD88 L265P (67%) and CD79B (63%), and CDKN2A deletions (83%). PCNSLs exhibit significantly more focal deletions of HLA-D (6p21) locus as a potential mechanism of immune evasion. Mutational signatures correlating with DNA replication and mitosis are significantly enriched in PCNSL. TERT gene expression is significantly higher in PCNSL compared to activated B-cell (ABC)-DLBCL. Transcriptome analysis clearly distinguishes PCNSL and systemic DLBCL into distinct molecular subtypes. Epstein-Barr virus (EBV)+ CNSL cases lack recurrent mutational hotspots apart from IG and HLA-DRB loci. We show that PCNSL can be clearly distinguished from DLBCL, having distinct expression profiles, IG expression and translocation patterns, as well as specific combinations of genetic alterations

The ESA Hera Mission : Detailed Characterization of the DART Impact Outcome and of the Binary Asteroid (65803) Didymos

Funding Information: To achieve these objectives, Milani is carrying two scientific payloads, the ASPECT visual and near-infrared (Vis-NIR) imaging spectrometer and the VISTA thermogravimeter aimed at collecting and characterizing volatiles and dust particles below 10 μm. Additionally, navigation payloads include a visible navigation camera and lidar. The Milani consortium is composed of entities and institutions from Italy, the Czech Republic, and Finland. The consortium Prime is Tyvak International, responsible for the whole program management and platform design, development, integration, testing, and final delivery to the customer. Politecnico di Torino is tasked with defining requirements and performing thermal, radiation, and debris analysis. Politecnico di Milano is responsible for mission analysis and GNC. Altec will support the Ground Segment architecture and interface definition. Centro Italiano per la Ricerca Aerospaziale (CIRA) is responsible for the execution of the vehicle environmental campaign. HULD contributes to developing the mission-specific software. VTT is the main payload (ASPECT hyperspectral imager) provider and is supported by the following entities dealing with ASPECT-related development: University of Helsinki (ASPECT calibration); Reaktor Space Lab (ASPECT Data Processing Unit development), Institute of Geology of the Czech Academy of Sciences (ASPECT scientific algorithms requirements and testing); and Brno University of Technology (ASPECT scientific algorithms development). INAF-IAPS is the secondary Payload (VISTA, dust detector) provider. Funding Information: The Mission PI is appointed by ESA and is the primary interface to ESA. The Hera SMB consists of the ESA Hera Project Scientist (ESA PS), the Mission PI, and the Hera Advisory Board, consisting of four mission advisors. The Mission PI chairs the HIT and is supported by the Hera Advisory Board. The tasks of the Hera SMB are 1. advising the Hera mission project team on all aspects related to the Hera mission objectives; 2. ensuring that the WGs’ activities cover the needs of the Hera mission; 3. providing recommendations to ESA concerning the membership in the HIT; and 4. implementing the Publication Policy. Funding Information: Hera is the ESA contribution to the AIDA collaboration. Hera, Juventas, Milani, and their instruments are developed under ESA contract supported by national agencies. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 870377 (project NEO-MAPP), the CNRS through the MITI interdisciplinary programs, ASI, CNES, JAXA, the Academy of Finland project no. 335595, and was conducted with institutional support RVO 67985831 of the Institute of Geology of the Czech Academy of Sciences. M.L., E.P., P.T .and E.D. are grateful to the Italian Space Agency (ASI) for financial support through Agreement No. 2022-8-HH.0 in the context of ESA’s Hera mission. We are grateful to the whole Hera team, including Working Group core members and other contributors for their continuous efforts and support. Their names can be found on the following website: https:// www.heramission.space/team. Publisher Copyright: © 2022. The Author(s). Published by the American Astronomical Society.Hera is a planetary defense mission under development in the Space Safety and Security Program of the European Space Agency for launch in 2024 October. It will rendezvous in late 2026 December with the binary asteroid (65803) Didymos and in particular its moon, Dimorphos, which will be impacted by NASA’s DART spacecraft on 2022 September 26 as the first asteroid deflection test. The main goals of Hera are the detailed characterization of the physical properties of Didymos and Dimorphos and of the crater made by the DART mission, as well as measurement of the momentum transfer efficiency resulting from DART’s impact. The data from the Hera spacecraft and its two CubeSats will also provide significant insights into asteroid science and the evolutionary history of our solar system. Hera will perform the first rendezvous with a binary asteroid and provide new measurements, such as radar sounding of an asteroid interior, which will allow models in planetary science to be tested. Hera will thus provide a crucial element in the global effort to avert future asteroid impacts at the same time as providing world-leading science.Peer reviewe

DNA methylome analysis in Burkitt and follicular lymphomas identifies differentially methylated regions linked to somatic mutation and transcriptional control

Although Burkitt lymphomas and follicular lymphomas both have features of germinal center B cells, they are biologically and clinically quite distinct. Here we performed whole-genome bisulfite, genome and transcriptome sequencing in 13 IG-MYC translocation-positive Burkitt lymphoma, nine BCL2 translocation-positive follicular lymphoma and four normal germinal center B cell samples. Comparison of Burkitt and follicular lymphoma samples showed differential methylation of intragenic regions that strongly correlated with expression of associated genes, for example, genes active in germinal center dark-zone and light-zone B cells. Integrative pathway analyses of regions differentially methylated in Burkitt and follicular lymphomas implicated DNA methylation as cooperating with somatic mutation of sphingosine phosphate signaling, as well as the TCF3-ID3 and SWI/SNF complexes, in a large fraction of Burkitt lymphomas. Taken together, our results demonstrate a tight connection between somatic mutation, DNA methylation and transcriptional control in key B cell pathways deregulated differentially in Burkitt lymphoma and other germinal center B cell lymphomas