Regulators of stream ecosystem recovery from disturbance

Doctor of PhilosophyDepartment of BiologyWalter K. DoddsStreams exist in a state of dynamic equilibrium with frequent floods and drought. The frequency and intensity of stream disturbances are projected to increase with greater water withdrawal for agriculture and biofuel production, watershed development, and altered climate. Changes in the hydrologic regime may alter stream ecosystems. I studied how stream communities return after disturbances and how nutrients, consumers, and substrata heterogeneity influence recovery trajectories. Large consumers were excluded from pools following a severe drought to assess how community structure and function returned in their absence. Large consumers reduced algal biomass, primary productivity, and nutrient uptake rates, and delayed macroinvertebrate recolonization. However, grazer effects were temporary and their influence weakened after five weeks. In a second experiment, I assessed the relative influence of grazer density and nutrient loadings on algal recovery from flood. Nutrients had a stronger effect on recovery than grazers, but the strength of each varied temporally. Grazer control decreased and nutrient control increased over time. A third experiment addressed the physical properties of stream substrata on algal development. The relationship among algal accumulation and substrata surface topography was assessed by growing algae on substrata with varying orientation and roughness. Total algal biomass decreased on surfaces with angles > 45 degrees, and peaked at an intermediate roughness (pit depth of [similar to]17 [Mu]m). Rougher surfaces collected more tightly attached (grazer resistant) forms and less loosely attached (grazer susceptible) forms. Individual algal forms responded differently to grazing pressure, nutrient availability, and surface features. I developed a method using Fourier-transform infrared microspectroscopy to measure single-cell physiological responses in benthic algae. Nutrients and consumers were strong regulators of ecosystem succession following disturbance, but nutrient influence was stronger. The influence of nutrients and consumers were context dependent, and changed over the course of recovery. Rougher surfaces increase algal growth and shifted algal assemblages to more grazer resistant forms, which may decrease the influence of large consumers on stream function. Altering the severity and frequency of disturbances can change the trajectory of stream recovery and ultimately change community composition and stream metabolic activity, which may alter ecosystem services such as water purification and recreation

Effects of the nuisance alga Didymosphenia geminata on benthic community resource use

Didymosphenia geminata (Didymo) is a benthic, freshwater diatom that has reached nuisance biomass levels in streams throughout the world. The stalks used to attach cells to substrata can form mats that completely cover stream bottoms and alter benthic community structure; however, it is unclear how Didymo influences energy flow in benthic food webs. We investigated the effects of Didymo mats on community resource use in the Clinch, South Holston, and Watauga rivers in Tennessee. We sampled each river in 2014 and 2015 along a spatial gradient downstream of dams that corresponds to a gradient of mat coverage. We measured stable carbon and nitrogen isotope ratios of major food web components to assess potential changes in macroinvertebrate and fish resource use due to didymo mat proliferation. In 2015, we also used lipid profiles to better isolate the effects of Didymo on resource use. Macroinvertebrate isotopic signatures consistently suggested shifts in resource use in the presence of high (>50%) Didymo coverage from rock biofilms to macrophytes. Brown and rainbow trout in both high and low Didymo sites appeared to primarily be assimilating materials from turbellarians and Didymo stalk material, with less input from amphipods and other macroinvertebrates. Lipid analyses from 2015 supported isotope results. Didymo mats change food resource availability and macroinvertebrate assemblages adapt to using what is available. Trout also appear to consume Didymo mats but do not change what they consume in low and high mat coverage stream reaches

Periphyton responses to nutrient and atrazine mixtures introduced through agricultural runoff

Agricultural runoff often contains pollutants with antagonistic impacts. The individual influence of nutrients and atrazine on periphyton has been extensively studied, but their impact when introduced together and with multiple agricultural pollutants is less clear. We simulated a field-scale runoff pulse into a riverine wetland that mimicked pollutant composition typical of field runoff of the Mississippi River Alluvial Plain. Periphyton biomass and functional responses were measured for 2 weeks along a 500 m section. Additionally, laboratory chamber assays were used to identify potential periphyton changes due to nutrients, atrazine, and their interactions. Generally, nutrients stimulated, and atrazine reduced chlorophyll a (Chl a) in chambers. In the wetland, nutrient and atrazine relationships with periphyton were weaker, and when found, were often opposite of trends in chambers. Total nitrogen (TN) was inversely related to Chl a, and total phosphorus was inversely related to respiration (R) rates. Atrazine (10–20 lg L-1 in the wetland) had a positive relationship with ash-free dry mass (AFDM), and weakened the relationship between TN and AFDM. Wetland periphyton biomass was better correlated to total suspended solids than nutrients or atrazine. Periphyton function was resilient as periphyton gross primary production (GPP)/R ratios were not strongly impacted by runoff. However, whole-system GPP and R decreased over the 2-week period, suggesting that although periphyton metabolism recovered quickly, whole-system metabolism took longer to recover. The individual and combined impacts of nutrients and atrazine in complex pollutant mixtures can vary substantially from their influence when introduced separately, and non-linear impacts can occur with distance downstream of the pollutant introduction point

Consumer Return Chronology Alters Recovery Trajectory of Stream Ecosystem Structure and Function Following Drought

Consumers are increasingly being recognized as important drivers of ecological succession, yet it is still hard to predict the nature and direction of consumer effects in nonequilibrium environments. We used stream consumer exclosures and large outdoor mesocosms to study the impact of macroconsumers (i.e., fish and crayfish) on recovery of intermittent prairie streams after drying. In the stream, macroconsumers altered system recovery trajectory by decreasing algal and macroinvertebrate biomass, primary productivity, and benthic nutrient uptake rates. However, macroconsumer influence was transient, and differences between exclosures and controls disappeared after 35 days. Introducing and removing macroconsumers after 28 days resulted mainly in changes to macroinvertebrates. In mesocosms, a dominant consumer (the grazing minnow Phoxinus erythrogaster) reduced macroinvertebrate biomass but had little effect on algal assemblage structure and ecosystem rates during recovery. The weak effect of P. erythrogaster in mesocosms, in contrast to the strong consumer effect in the natural stream, suggests that both timing and diversity of returning consumers are important to their overall influence on stream recovery patterns. Although we found that consumers significantly altered ecosystem structure and function in a system experiencing rapid changes in abiotic and biotic factors following disturbance, consumer effects diminished over time and trajectories converged to similar states with respect to primary producers, in spite of differences in consumer colonization history. Thus, consumer impacts can be substantial in recovering ecosystems and are likely to be dependent on the disturbance regime and diversity of the consumer community

Mitigating agrichemicals from an artificial runoff event using a managed riverine wetland

We examined the mitigation efficiency of a managed riverine wetland amended with a mixture of suspended sediment, two nutrients (nitrogen and phosphorus), and three pesticides (atrazine, metolachlor, and permethrin) during a simulated agricultural runoff event. Hydrologic management of the 500 m-long, 25 mwide riverine wetland was done by adding weirs at both ends. The agrichemical mixture was amended to the wetland at the upstream weir simulating a four-hour, ~1 cm rainfall event from a 16 ha agricultural field. Water samples (1 L) were collected every 30 min within the first 4 h, then every 4 h until 48 h, and again on days 5, 7, 14, 21, and 28 post-amendment at distances of 0 m, 10 m, 40 m, 300 m and 500 m from the amendment point within the wetland for suspended solids, nutrient, and pesticide analyses. Peak sediment, nutrient, and pesticide concentrations occurred within 3 h of amendment at 0 m, 10 m, 40 m, and 300 m downstream and showed rapid attenuation of agrichemicals from the water column with 79–98%, 42–98%, and 63–98% decrease in concentrations of sediments, nutrients, and pesticides, respectively, within 48 h. By day 28, all amendments were near or below pre-amendment concentrations. Water samples at 500 m showed no changes in sediment or nutrient concentrations; pesticide concentrations peaked within 48 h but at ≤11% of upstream peak concentrations and had dissipated by day 28. Managed riverine wetlands≥1 ha and with hydraulic residence times of days to weeks can efficiently trap agricultural runoff during moderate (1 cm) late-spring and early-summer rainfall events, mitigating impacts to receiving rivers

The Renormalization Group and Singular Perturbations: Multiple-Scales, Boundary Layers and Reductive Perturbation Theory

Perturbative renormalization group theory is developed as a unified tool for global asymptotic analysis. With numerous examples, we illustrate its application to ordinary differential equation problems involving multiple scales, boundary layers with technically difficult asymptotic matching, and WKB analysis. In contrast to conventional methods, the renormalization group approach requires neither {\it ad hoc\/} assumptions about the structure of perturbation series nor the use of asymptotic matching. Our renormalization group approach provides approximate solutions which are practically superior to those obtained conventionally, although the latter can be reproduced, if desired, by appropriate expansion of the renormalization group approximant. We show that the renormalization group equation may be interpreted as an amplitude equation, and from this point of view develop reductive perturbation theory for partial differential equations describing spatially-extended systems near bifurcation points, deriving both amplitude equations and the center manifold.Comment: 44 pages, 2 Postscript figures, macro \uiucmac.tex available at macro archives or at ftp://gijoe.mrl.uiuc.edu/pu

Microclimate variables of the ambient environment deliver the actual estimates of the extrinsic incubation period of Plasmodium vivax and Plasmodium falciparum : A study from a malaria-endemic urban setting, Chennai in India

Background: Environmental factors such as temperature, relative humidity and their daily variation influence a range of mosquito life history traits and hence, malaria transmission. The standard way of characterizing environmental factors with meteorological station data need not be the actual microclimates experienced by mosquitoes within local transmission settings. Methods: A year-long study was conducted in Chennai, India to characterize local temperature and relative humidity (RH). Data loggers (Hobos) were placed in a range of probable indoor and outdoor resting sites of Anopheles stephensi. Recordings were taken hourly to estimate mean temperature and RH, together with daily temperature range (DTR) and daily relative humidity range. The temperature data were used to explore the predicted variation in extrinsic incubation period (EIP) of Plasmodium falciparum and Plasmodium vivax between microhabitats and across the year. Results: Mean daily temperatures within the indoor settings were significantly warmer than those recorded outdoors. DTR in indoor environments was observed to be modest and ranged from 2 to 6 °C. Differences in EIP between microhabitats were most notable during the hottest summer months of April-June, with parasite development predicted to be impaired for tiled houses and overhead tanks. Overall, the prevailing warm and stable conditions suggest rapid parasite development rate regardless of where mosquitoes might rest. Taking account of seasonal and local environmental variation, the predicted EIP of P. falciparum varied from a minimum of 9.1 days to a maximum of 15.3 days, while the EIP of P. vivax varied from 8.0 to 24.3 days. Conclusions: This study provides a detailed picture of the actual microclimates experienced by mosquitoes in an urban slum malaria setting. The data indicate differences between microhabitats that could impact mosquito and parasite life history traits. The predicted effects for EIP are often relatively subtle, but variation between minimum and maximum EIPs can play a role in disease transmission, depending on the time of year and where mosquitoes rest. Appropriate characterization of the local microclimate conditions would be the key to fully understand the effects of environment on local transmission ecology

Sediment Nutrient Flux Rates in a Shallow, Turbid Lake Are More Dependent on Water Quality Than Lake Depth

The bottom sediments of shallow lakes are an important nutrient sink; however, turbidity may alter the influence of water depth on sediment nutrient uptake by reducing light and associated oxic processes, or altering nutrient availability. This study assessed the relative influence of water quality vs. water depth on sediment nutrient uptake rates in a shallow agricultural lake during spring, when sediment and nutrient loading are highest. Nitrate and soluble reactive phosphorus (SRP) flux rates were measured from sediment cores collected across a depth and spatial gradient, and correlated to water quality. Overlying water depth and distance to shore did not influence rates. Both nitrate and SRP sediment uptake rates increased with greater Secchi depth and higher water temperature, and nitrate and SRP rates increased with lower water total N and total P, respectively. The importance of water temperature on N and P cycling was confirmed in an additional experiment; however, different patterns of nitrate reduction and denitrification suggest that alternative N2 production pathways may be important. These results suggest that water quality and temperature can be key drivers of sediment nutrient flux in a shallow, eutrophic, turbid lake, and water depth manipulation may be less important for maximizing spring runoff nutrient retention than altering water quality entering the lake

Dynamic influences of nutrients and grazing fish on periphyton during recovery from flood.

Nutrients and grazers both can regulate benthic algal structure and function in streams, but the relative strength of each factor depends on stream biotic and abiotic conditions. The abundance of stream organisms and nutrient availability can change rapidly after a flood. Thus, nutrient and grazer influences on algal development and how these drivers interact may vary temporally during recovery. We measured benthic structural and functional development for 35 d after a simulated flood in large outdoor mesocosms under a gradient of 6 nutrient loadings crossed with 6 densities of grazing fish (Southern redbelly dace, Phoxinus erythrogaster). Nutrients influenced algal development more than dace did and were better correlated with algal function (area-specific primary productivity and nutrient uptake) than with structure (biomass). Dace influenced all structural variables and biomass-specific gross primary productivity, but their influence was relatively weak and was observed only early in recovery. Dace influence weakened and nutrient influence strengthened during recovery. Understanding context-dependent relationships in postdisturbance community dynamics is essential for predicting ecosystem responses to future changes in nutrient inputs and biodiversity, particularly in systems, such as headwater streams, with frequent disturbance