Survey and surveillance on rice false smut disease severity in Tamil Nadu and the influence of wind velocity on disease progression under field conditions

The false smut disease of rice incited by Ustilaginoidea virens is a major constraint on rice production. The main aim of the present study was to ascertain the false smut disease severity in both delta and non-delta districts of Tamil Nadu through survey and surveillance and assess the correlation between the disease severity and wind velocity for the past three years, 2019, 2020 and 2021. Moreover, the present study addressed the disease distribution pattern of false smut diseases under field conditions.  The results obtained from survey results revealed that the maximum disease severity was recorded in Nagapattinam district (Nagapattinam block) with 27.45% and the minimum disease severity was recorded in the district Theni (Bodinayakanur block) with 8% in 2021. Similarly in 2019 and 2020 maximum disease severity was recorded in the following districts Thanjavur district (Orathanadu block) with 19.91% and Thanjavur district (Peravurani block) with 18.54% and the minimum disease severity was recorded in the following districts Madurai district (Madurai north block) with 4.78% and Madurai district (Usilampatti block) with 4.78% respectively. The obtained R2 values through regression analysis were 0.70, 0.79 and 0.76 in the following years, 2019, 2020 and 2021, respectively. Besides the relationship between the false smut disease development, the pattern wind direction was also assessed. By assessing the false smut disease distribution pattern under field conditions, more disease distribution was observed around the surrounding area of the paddy field as well as the diagonal path of the field which clearly revealed that wind direction influences the disease development

Risk prediction models for melanoma: a systematic review.

Melanoma incidence is increasing rapidly worldwide among white-skinned populations. Earlier diagnosis is the principal factor that can improve prognosis. Defining high-risk populations using risk prediction models may help targeted screening and early detection approaches. In this systematic review, we searched Medline, EMBASE, and the Cochrane Library for primary research studies reporting or validating models to predict risk of developing cutaneous melanoma. A total of 4,141 articles were identified from the literature search and six through citation searching. Twenty-five risk models were included. Between them, the models considered 144 possible risk factors, including 18 measures of number of nevi and 26 of sun/UV exposure. Those most frequently included in final risk models were number of nevi, presence of freckles, history of sunburn, hair color, and skin color. Despite the different factors included and different cutoff values for sensitivity and specificity, almost all models yielded sensitivities and specificities that fit along a summary ROC with area under the ROC (AUROC) of 0.755, suggesting that most models had similar discrimination. Only two models have been validated in separate populations and both also showed good discrimination with AUROC values of 0.79 (0.70-0.86) and 0.70 (0.64-0.77). Further research should focus on validating existing models rather than developing new ones.This report is independent research arising from a Clinician Scientist award supported by the National Institute for Health Research (RG 68235) and J Usher-Smith is funded by a National Institute for Health Research Clinical LectureshipThis is the author accepted manuscript. The advanced access published version can be found on the publisher's website at: http://cebp.aacrjournals.org/content/early/2014/06/03/1055-9965.EPI-14-0295.abstrac

Integrating improved goat breeds with new varieties of sweet potatoes and cassava in the agro-pastoral systems of Tanzania: A gendered analysis

This paper focuses on analysing gender issues in livestock and root crop production. Qualitative data for the study was collected through gender disaggregated group discussions (GDs) in two districts, Mvomero and Kongwa in Tanzania. Data were collected from 4 communities representing the four villages—Wami Luhindo and Kunke in Mvomero, and Masinyeti and Ihanda in Kongwa respectively. A total of 12 GDs were conducted involving 224 men and women who participated in the GDs. The qualitative data were analysed descriptively and by using measures such as percentages, tables, pie, bar charts and Venn diagrams. The study found that men owned all the goats and made all goat related decisions unilaterally. Women were by far less likely than men to own not only goats, but also livestock in general. On management of goats, an important set of differences in activities is associated with gender as well as with age. Changing livelihood opportunities such as rural–urban migration were identified as a factor that influenced gender and goat management and this increases workload for women. On decision-making over goats, women have limited control over decisions on sale and use of incomes generated from sale of goats. Ownership of crops between men and women is quite distinct, and depends on the market prices of crops and yield of the crop they grow for cash. Men own cash crops or crops for cash whereas women own subsistence or food crops for home consumption. Wealth status had a direct relation with individual decision-making on crops, rich men and women decided on what crops to cultivate, whereas non-rich farmers worked on wealthy farmers’ farms as casual labourers. Perceptions of women and men on the potential benefits of integrating root crops and goat varied. Men perceived value addition resulting from owning dairy goats and the attendant increase in income for them whereas women perceived change in status quo and increase workload resulting from stall goat management activities. Some participants explained that the anticipated increase in men’s real income could come about either through men accessing women’s income, or more commonly, women using their earnings to substitute men’s expenditure on household needs and children’s education. The study concluded that there are gender differences on perceived potential of integrating root crops and dairy goat production, as prevailing tradition may limit women’s participation. The beneficiaries expressed the timeliness of CGP project and exhibited willingness to partake in the project and ensure equitable benefits for participants if participatory gender training and awareness is ensured at both the household and community level. The study recommended that investing in rigorous gender trainings for both women and men, to initially sensitize them on the importance of including both women and men in development projects and sustain their continued understanding of the importance of gender inclusive activities

Efficient Contextualism

This Article recommends an economic methodology of contract interpretation that enables the court to maximize the benefits of exchange for the parties and thereby enhance the institution of contracting. We recommend a methodology that asks the parties to identify the determinants of a surplus maximizing interpretation so that the court can determine whether the determinants raise issues that need to be tried. We thus avoid the false choice between textualist and contextualist methodologies, while allowing the parties and the court to avoid costly litigation. For textualist courts, our methodology helps the judge determine when the terms the parties used are ambiguous enough to require the court to consider context. For contextualist courts, it streamlines the interpretive inquiry by identifying which contextual facts are important and why, which allows courts to avoid or streamline trials. Our method therefore allows courts to avoid the problems of textualism (which can make easy cases difficult) and anything-goes-contextualism (which can make difficult cases unmanageable).Our methodology reflects a model of bargaining that emphasizes the divergent interests and preferences of the parties. Although both parties seek to minimize the costs of contracting, the parties have divergent views about those costs and about the tradeoffs each must make to minimize those costs. Accordingly, we deny that courts can find the meaning of a disputed term in the intent of the parties. Instead, we believe that courts must identify (a) the set of obligations that, in the context of the parties’ private projects and undisputed terms, increase contractual surplus and (b) the party who is in the best position to avoid the dispute (and thus lower the cost of contracting) by identifying the terms on which the parties disagree ex ante.We present a structured analytical framework that courts and other enforcers should use to determine which interpretation offered by the parties maximizes the surplus, given the undisputed terms of the contract and the bargaining position of the parties. Courts and other enforcers should not try to influence how other contractors act, except by faithfully determining the surplus maximizing interpretation; nor should they seek to determine what obligations other contracting parties might have undertaken, nor on how hypothetical bargainers might have bargained

Identification of a major causative agent of human cercarial dermatitis, Trichobilharzia franki (Muller and Kimmig 1994), in southern England and its evolutionary relationships with other European populations.

Background Trichobilharzia is the most species rich and widely distributed genus of schistosomes and is known throughout Europe and North America as an agent of human cercarial dermatitis. The disease is caused by an acute allergic reaction in the skin that develops as a consequence of repeated contact with water containing schistosomatid cercariae. However, despite historical outbreaks of the disease, there are no published records of accurately identified Trichobilharzia species from the UK. Methods Two hundred Radix auricularia (L.) were sampled from a recreational fishing lake in Hampshire and emerging schistosomatid cercariae were collected for microscopy and DNA extraction. General morphological description of the cercariae was performed, alongside sequencing and phylogenetic analysis of the 28S ribosomal DNA for accurate species identification as well as comparisons of ITS1 in order to identify evolutionary affinities with other European populations. All molecular comparisons were performed using published sequences. Results The phylogenetic analysis of 28S sequences identified the cercariae as Trichobilharzia franki. Two unique British ITS1 haplotypes were identified which were most closely related to haplotypes of T. franki populations from France. Haplotype network analysis indicated the mixing of T. franki populations throughout Europe. It is suggested that parasite distribution is the probable result of the movement of migratory waterfowl. Conclusions This is the first accurate record of T. franki in the UK. The movement of T. franki with waterfowl could pose a considerable human health risk, as in mainland Europe, and signifies T. franki-associated human cercarial dermatitis as a re-emerging disease in the UK

Receptor chimeras demonstrate that the C-terminal domain of the human cytomegalovirus US27 gene product is necessary and sufficient for intracellular receptor localization

<p>Abstract</p> <p>Background</p> <p>Human cytomegalovirus (HCMV) is ubiquitous in the population but generally causes only mild or asymptomatic infection except in immune suppressed individuals. HCMV employs numerous strategies for manipulating infected cells, including mimicry of G-protein coupled receptors (GPCRs). The HCMV US27 gene product is a putative GPCR, yet no ligand or signaling has been identified for this receptor. In the present study, immunofluorescence microscopy was used to examine the cellular distribution of wild type US27, as well as US27 deletion mutants and chimeric receptors.</p> <p>Results</p> <p>In transiently transfected cells, wild type US27 was found primarily in intracellular compartments, in striking contrast to the cell surface distribution seen for the human cellular chemokine receptor CXCR3. When the N-terminal extracellular domains of the two receptors were swapped, no change in protein localization was observed. However, swapping of the C-terminal intracellular domains resulted in a significant change in receptor distribution. A chimera that contained US27 fused to the C-terminal intracellular tail of CXCR3 exhibited surface distribution similar to that of wild-type CXCR3. When the C-terminal domain of US27 was fused to CXCR3, this chimeric receptor (CXCR3/US27-CT) was found in the same intracellular pattern as wild-type US27. In addition, a US27 mutant lacking the C-terminus (US27ΔCT) failed to accumulate inside the cell and exhibited cell surface distribution. Co-localization with organelle-specific markers revealed that wild-type US27 was found predominantly in the Golgi apparatus and in endosomal compartments, whereas the US27/CXCR3-CT chimera, US27ΔCT and US27Δ348 mutants were not localized to endosomal compartments.</p> <p>Conclusions</p> <p>The results indicate that the C-terminal domain of the HCMV US27 protein, which contains a di-leucine endocytic sorting motif, is both necessary and sufficient for intracellular localization, which may also help explain why no cellular ligands have yet been identified for this viral receptor.</p

Transcriptomic effects of dispersed oil in a non-model decapod crustacean

Background. Oil spills are major environmental disasters. Dispersants help control spills, as they emulsify oil into droplets to speed bioremediation. Although dispersant toxicity is controversial, the genetic consequences and damages of dispersed oil exposure are poorly understood. We used RNA-seq to measure gene expression of flatback mudcrabs (Eurypanopeus depressus, Decapoda, Brachyura, Panopeidae) exposed to dispersed oil. Methods. Our experimental design included two control types, oil-only, and oil-dispersant treatments with three replicates each. We prepared 100 base pair-ended libraries from total RNA and sequenced them in one Illumina HiSeq2000 lane. We assembled a reference transcriptome with all replicates per treatment, assessed quality with novel metrics, identified transcripts, and quantified gene expression with open source software. Results. Our mudcrab transcriptome included 500,008 transcripts from 347,082,962 pairend raw reads. In oil-only treatments, we found few significant differences. However, in oildispersant treatments, over 4000 genes involved with cellular differentiation, primordial cellular component upkeep, apoptosis, and immune response were downregulated. A few muscle structure and development genes were upregulated. Discussion. Our results provide evidence that exposure to chemically dispersed oil causes a generalized cellular shutdown and muscular repair attempts. Our results suggest current oil-spill treatment procedures could be detrimental to crustaceans and indicate additional research is needed to evaluate the impact of oil spills in gene expression. Finally, traditional quality metrics such as N50s have limitations to explain the nature of RNA-seq compared to new methods in non-model decapod crustaceans

Understanding Sanctuary Cities

In the wake of President Trump’s election, a growing number of local jurisdictions around the country have sought to disentangle their criminal justice apparatus from federal immigration enforcement efforts. These localities have embraced a series of reforms that attempt to ensure immigrants are not deported when they come into contact with the criminal justice system. The Trump administration has labeled these jurisdictions “sanctuary cities” and vowed to “end” them by, among other things, attempting to cut off their federal funding. This Article is a collaborative project authored by law professors specializing in the intersection between immigration and criminal law. In it, we set forth the central features of the Trump administration’s mass deportation plans and its campaign to “crack down” on sanctuary cities. We then outline the diverse ways in which localities have sought to protect their residents by refusing to participate in the Trump immigration agenda. Such initiatives include declining to honor immigration detainers, precluding participation in joint operations with the federal government, and preventing immigration agents from accessing local jails. Finally, we analyze the legal and policy justifications that local jurisdictions have advanced. Our examination reveals important insights for how sanctuary cities are understood and preserved in the age of Trump

Diversity Of Diagnoses And Student Learning Experiences In An On-Campus School Psychology Assessment Center: Future Directions And Focus

School Psychology Assessment Centers (SPAC) developed at the University-level are crucial in developing competent school psychologists. While many models on how to develop mental health centers are in existence, a new model exists for creating a SPAC on a University campus that is specifically designed to serve the diverse surrounding community and postsecondary students (see Ellis-Hervey et al., 2016). The community’s needs were reviewed, including missing service links in the region, and identification of potential clients who would benefit most from services provided. Senior students who work with clients in the SPAC gain assessment skills and build supervision skills through close interactions and consultation with junior students. New goals of the SPAC is to develop and implement academic, behavioral, social skills and therapy/counseling services