9 research outputs found
Poly(amido amine)-Based Mannose-Glycodendrimers As Multielectron Redox Probes for Improving Lectin Sensing
An easy-to-prepare series of electroactive polyÂ(amido
amine) (PAMAM)-based
dendrimers of generations G0 to G2 having mannopyranosylferrocenyl
moieties in the periphery to detect carbohydrateâprotein interactions
is reported. The synthesis involved the functionalization of the PAMAM
surface with azidomethylferrocenyl groups and subsequent coupling
of mannoside units by the CuÂ(I)-catalyzed Huisgen reaction. The binding
affinity of the series of electroactive glycodendrimers was studied
by isothermal titration calorimetry (ITC) and differential pulse voltammetry
(DPV). Upon complexation of the glycodendrimers conjugates with prototypical
concanavalin A (Con A), voltammograms showed a decrease of the peak
current. Such dendrimers showed a notable improvement of redox sensing
abilities toward Con A when compared with mono- and divalent analogues,
based on both the glycoside multivalent and ferrocene dendritic effects
Ferroceneâmannose conjugates as electrochemical molecular sensors for concanavalin A lectin
ÎČâCyclodextrin-Bearing Gold Glyconanoparticles for the Development of Site Specific Drug Delivery Systems
Three novel gold nanoparticles containing
multiple long, flexible
linkers decorated with lactose, ÎČ-cyclodextrin, and both simultaneously
have been prepared. The interaction of such nanoparticles with ÎČ-d-galactose-recognizing lectins peanut agglutinin (PNA) and
human galectin-3 (Gal-3) was demonstrated by UVâvis studies.
Gal-3 is well-known to be overexpressed in several human tumors and
can act as a biorecognizable target. This technique also allowed us
to estimate their loading capability toward the anticancer drug methotrexate
(MTX). Both results make these glyconanoparticles potential site-specific
delivery systems for anticancer drugs