Blackspot seabream early life stages dispersal by hydrodynamic modelling (Strait of Gibraltar - Alboran Sea)

The Blackspot seabream (Pagellus bogaraveo) is a commercially appreciated demersal fish, widespread in the north-eastern Atlantic and middle-western Mediterranean. The Strait of Gibraltar (SoG) is an important fishing area where artisanal fleets from Spain and Morocco target this species using special longline gears known as “voracera”. Different studies on the health status of this species claim an overexploitation of the resource without any kind of objective management measures implemented yet by the associated countries. Improving the knowledge of the population dynamics, and particularly during the spawning window, is crucial for a better management of the fishing effort for this species. There is an agreement in literature in considering the SoG as an especially energetic and dispersive spawning zone for this species. Once spawned, eggs and larvae (ELS) are assumed to be scattered by the currents towards both sides of the SoG, mostly to the eastwards Alboran basin, where high concentrations of juveniles occur. A high resolution hydrodynamic model coupled to a Lagrangian particle tracking system is employed to assess the potential dispersal pathways of blackspot seabream ELS spawned in the SoG. Recursive releases of passive tracers in different virtual spawning spots and depths within the SoG region are tracked under different tidal conditions in order to obtain an overall characterization of the spatial dispersion patterns of the studied species. Semidiurnal tidal currents emerge as the primary factor in determining the horizontal dispersion and pathway of the spawning products, although the spring-neap tidal cycle and the spawning depth can be also important variables depending on the region considered as well as the arrival zone for the ELS dispersal paths over the Alboran SeaUniversidad de Málaga. Campus de Excelencia Internacional CEIMA

Hydrodinamic connectivity and dispersal patterns in the Strait of Gibraltar: implications for a transboundary species

The blackspot seabream is a highly appreciated species that has an important target of the Spanish and Moroccan fisheries in the transcontinental waters of the Strait of Gibraltar area (Gil, 2006). It is also one of the most exploited resources of the region, which has led to a notable drop of catches over the years, arriving to a vulnerable status nowadays. Consequently, a reduction of fishing mortality towards sustainability levels was recommended (GFCM, 2021). Improving our knowledge on the interaction of this species with its physical environment is a key issue in the efforts towards rebuilding the fishery target population until its maximum sustainable yield (Cowen et al., 2006). To gain insight into this interaction, a high-resolution circulation model coupled to a lagrangian tracking module has been employed, using eggs and larvae (early-life-stages, ELS) as purely passive particles advected by simulated currents. Several spawning scenarios consisting of different temporal (tidal phase and strength) and spatial (depths and sites) initial conditions have been analyzed to identify the most likely pathways of ELS dispersion. Eastward transport by the Atlantic Jet exiting the Strait of Gibraltar is the most influencing process in that dispersion. Regarding temporal fluctuations, fortnightly tidal modulation appears to be the prevailing factor determining the horizontal paths of ELS, being the spring tide responsible of the greatest scattering of eggs and larvae. Interestingly, the spatial distribution numerically simulated is consistent with results of larvae distribution obtained from samples collected during recent scientific surveys. The results presented in this study can be certainly different attending to the species strategy. The role of other important larval traits not included in this study requires also further research that may help decision-makers to establish biological rest periods that lead to maximize connectivity and sustainability

Gibberellin–Abscisic Acid Balances during Arbuscular Mycorrhiza Formation in Tomato

Plant hormones have become appropriate candidates for driving functional plant mycorrhization programs, including the processes that regulate the formation of arbuscules in arbuscular mycorrhizal (AM) symbiosis. Here, we examine the role played by ABA/GA interactions regulating the formation of AM in tomato. We report differences in ABA and GA metabolism between control and mycorrhizal roots. Active synthesis and catabolism of ABA occur in AM roots. GAs level increases as a consequence of a symbiosis-induced mechanism that requires functional arbuscules which in turn is dependent on a functional ABA pathway. A negative interaction in their metabolism has been demonstrated. ABA attenuates GA-biosynthetic and increases GA-catabolic gene expression leading to a reduction in bioactive GAs. Vice versa, GA activated ABA catabolism mainly in mycorrhizal roots. The negative impact of GA3 on arbuscule abundance in wild-type plants is partially offset by treatment with ABA and the application of a GA biosynthesis inhibitor rescued the arbuscule abundance in the ABA-deficient sitiens mutant. These findings, coupled with the evidence that ABA application leads to reduce bioactive GA1, support the hypothesis that ABA could act modifying bioactive GA level to regulate AM. Taken together, our results suggest that these hormones perform essential functions and antagonize each other by oppositely regulating AM formation in tomato roots.This study was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (CICYT) and Fondos Europeos de Desarrollo Regional (FEDER) through the Ministerio de Economía y Competitividad in Spain (AGL2008-00742; AGL2011-25930) as well as the Junta de Andalucía (Research Group BIO 260). JM-R was supported by a research fellowship from the FPU-MICINN program. The work on hormone measurements was funded by a Grant Agency of the Czech Republic (grant no. 14-34792S) and by the Ministry of Education, Youth and Sports of the Czech Republic through the National Program for Sustainability I (Nr. LO1204).Peer reviewedPeer Reviewe

Hepatocellular carcinoma risk-stratification based on ASGR1 in circulating epithelial cells for cancer interception

Purpose: Lack of diagnostic and prognostic biomarkers in hepatocellular carcinoma impedes stratifying patients based on their risk of developing cancer. The aim of this study was to evaluate phenotypic and genetic heterogeneity of circulating epithelial cells (CECs) based on asialoglycoprotein receptor 1 (ASGR1) and miR-122-5p expression as potential diagnostic and prognostic tools in patients with hepatocellular carcinoma (HCC) and liver cirrhosis (LC). Methods: Peripheral blood samples were extracted from LC and HCC patients at different disease stages. CECs were isolated using positive immunomagnetic selection. Genetic and phenotypic characterization was validated by double immunocytochemistry for cytokeratin (CK) and ASGR1 or by in situ hybridization with miR-122-5p and CECs were visualized by confocal microscopy. Results: The presence of CECs increased HCC risk by 2.58-fold, however, this was only significant for patients with previous LC (p = 0.028) and not for those without prior LC (p = 0.23). Furthermore, the number of CECs lacking ASGR1 expression correlated significantly with HCC incidence and absence of miR-122-5p expression (p = 0.014; r = 0.23). Finally, overall survival was significantly greater for patients at earlier cancer stages (p = 0.018), but this difference was only maintained in the group with the presence of CECs (p = 0.021) whereas progression-free survival was influenced by the absence of ASGR1 expression. Conclusion: Identification and characterization of CECs by ASGR1 and/or miR- 122-5p expression may be used as a risk-stratification tool in LC patients, as it was shown to be an independent prognostic and risk-stratification marker in LC and early disease stage HCC patients

Higher Order Dynamic Mode Decomposition of an experimental trailing vortex

The decay of trailing vortices is a fundamental problem in fluid mechanics and constitutes the basis of control applications that intend to alleviate the wake hazard. In order to progress, we use the recently developed modal-decomposition technique to identify the governing dynamics in an experimental trailing vortex. A particular emphasis is on the difficulty and usefulness of applying such tools to noisy experimental data. We conducted a water-tunnel experiment at a chord-based Reynolds number Re = 4 x 10^4 using stereoscopic particle image velocimetry measurements over a downstream range of 36 chords. The downstream evolution of the maximum of vorticity suggests that the whole wake can be partitioned into three consecutive regimes. A higher-order dynamic mode decomposition of the streamwise vorticity in each such part of the wake shows that the decay is well approximated by at most three modes. Additionally, our study provides evidence for the existence of several instabilities after the vortex roll up beyond about 6.5 chords

Newly impaired glucose metabolism and prognosis after percutaneous revascularization

Background: Clinical practice guidelines recommend ad hoc screening of diabetes in patients admitted for macrovascular disease; however, these recommendations are rarely followed in real practice. This study was undertaken to assess whether impaired glucose metabolism, newly diagnosed after percutaneous coronary intervention (PCI) or known diabetes, provides prognostic information. Methods: We studied 374 patients who underwent PCI. An oral glucose tolerance test was carried out in the known non-diabetic patients with fasting glucose < 7 mmol/L. Results: Eighty-one percent of the patients presented impaired glucose metabolism, from which 35.3% were previously diagnosed with diabetics, 21.4% were newly detected diabetics, and 24.3% were pre-diabetics. After a mean follow-up of 35.8 ± 13.4 months, only a known history of diabetes was an independent predictor of revascularization (OR = 2.03, p = 0.025), non-fatal acute myocardial infarction (OR = 2.70, p = 0.029) and readmission due to heart failure during the follow-up (OR = 3.82, p = 0.022). Conclusions: Screening for impaired glucose metabolism after PCI permits the detection of a high proportion of patients with abnormal glucose regulations. However, previously known diabetes remains the only independent predictor of cardiovascular events in the follow-up.

Transcription factors Sp1 and p73 control the expression of the proapoptotic protein NOXA in the response of testicular embryonal carcinoma cells to cisplatin

Testicular germ cell tumors (TGCTs) are highly responsive to and curable by cisplatin-based chemotherapy even in advanced stages. We have studied the molecular mechanisms involved in the induction of apoptosis in response to cisplatin, and found that proapoptotic Noxa is transcriptionally up-regulated following cisplatin exposure, even in the absence of p53, in NTERA2 cisplatin-sensitive cells but not in 1411HP-resistant cells. Blockade of Noxa reduced the apoptotic response of embryonal carcinoma (EC) NTERA2 cells to cisplatin. A detailed analysis of the Noxa promoter revealed that p73 and Sp1-like factors, Sp1 and KLF6, played key roles in the transcriptional control of this gene. Overexpression of TAp73 induced Noxa whereas the dominant negative isoform ΔNp73, reduced the levels of Noxa after cisplatin exposure in NTERA2 and 2102EP. Interestingly, down-regulation of Sp1 increased Noxa expression in response to cisplatin. However, blockade of KLF6 decreased cisplatin-induced up-regulation of Noxa in EC cell lines. In addition, tissue microarray analyses of TGCTs revealed that expression of Noxa correlates with good clinical prognosis in patients with embryonal carcinoma. Thus, our data show the transcriptional network that regulates Noxa in EC cells, which is key for their apoptotic response to cisplatin-based chemotherapy, and propose Noxa as a predictive factor of therapeutic response

Desarrollo de tecnologías para evaluar la funcionalidad de pacientes con prótesis de rodilla y su uso para el seguimiento clínico post-comercialización de productos sanitarios y la estimación de indicadores de gestión hospitalaria

Gomez Pellin, A.; Pedrero, J.; Peris Serra, JL.; Sinovas, I.; Atienza Vicente, CM.; Garrido Jaen, JD.; Bermejo Bosch, I.... (2019). Desarrollo de tecnologías para evaluar la funcionalidad de pacientes con prótesis de rodilla y su uso para el seguimiento clínico post-comercialización de productos sanitarios y la estimación de indicadores de gestión hospitalaria. Innovación biomecánica en Europa. (8):1-4. http://hdl.handle.net/10251/167980S14

Influence of Gender in Advanced Heart Failure Therapies and Outcome Following Transplantation

Biological differences between males and females change the course of different diseases and affect therapeutic measures' responses. Heart failure is not an exception to these differences. Women account for a minority of patients on the waiting list for heart transplantation or other advanced heart failure therapies. The reason for this under-representation is unknown. Men have a worse cardiovascular risk profile and suffer more often from ischemic heart disease. Conversely, transplanted women are younger and more frequently have non-ischemic cardiac disorders. Women's poorer survival on the waiting list for heart transplantation has been previously described, but this trend has been corrected in recent years. The use of ventricular assist devices in women is progressively increasing, with comparable results than in men. The indication rate for a heart transplant in women (number of women on the waiting list for millions of habitants) has remained unchanged over the past 25 years. Long-term results of heart transplants are equal for both men and women. We have analyzed the data of a national registry of heart transplant patients to look for possible future directions for a more in-depth study of sex differences in this area. We have analyzed 1-year outcomes of heart transplant recipients. We found similar results in men and women and no sex-related interactions with any of the factors related to survival or differences in death causes between men and women. We should keep trying to approach sex differences in prospective studies to confirm if they deserve a different approach, which is not supported by current evidence

On the thermal stability of the nanostructured tungsten coatings

Tungsten is a candidate to be used as plasma facing materials in future fusion nuclear reactors. There, the material has to withstand large radiation fluxes and thermal loads. Nowadays, nanostructured tungsten (NW) seems to exhibit a better radiation-resistance than the coarse grained. However, the thermal stability of NW is still an open question. On these bases, the thermal stability of NW coatings is studied in the temperature range from 1000 to 1473 K. For this purpose, Samples were isothermally annealed in vacuum at temperatures from 298 to 1473 K. The morphological and microstructural properties of the samples were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and X-Ray diffraction (XRD), respectively. For T 100 K, nanostructures start to grow in a bimodal fashion with activation energy of 0259 eV, reaching a submicron-sized threshold at T approximate to 1473 K