Start-up of the mesophilic anaerobic co-digestion of two-phase olive-mill waste and cattle manure using volatile fatty acids as process control parameter

In this work, the start-up and stabilization stages of mesophilic anaerobic co-digestion of 2POMW and CM in a semi-continuously fed stirred tank reactor (SSTR) were analyzed. Volatile fatty acids (VFAs) were monitored and used as the main control parameter for the start-up and stabilization stages, as well as to evaluate the potential inhibition episodes. The results showed that accumulation of propionic acid was the key factor in the inhibition of the methanogenic phase, leading to process imbalance. To avoid the problems associated with inhibition by high VFA concentrations, several reinoculations were performed using a suitable inoculum adapted to VFA degradation. The start-up phase was carried out in batch conditions for 97 days, reaching a final concentration of propionic acid of 12.77 mg/L. From that moment, the reactor was fed in a semi-continuous mode with a hydraulic retention time (HRT) of 40 days. A total period of 140 days was required to achieve a stable performance of the reactor with a methane productivity of 0.34 LCH4/LRd

Validación del Münchner Alkoholismus Test Subjektiv para el diagnóstico de alcoholismo en población adulta

ResumenObjetivoValidar la parte subjetiva del cuestionario MALT (Münchner Alkoholismus Test) para el diagnóstico de alcoholismo en población adulta.DiseñoEstudio descriptivo de validación de un instrumento diagnóstico.EmplazamientoDos centros de atención primaria, un centro de drogodependencias y un centro de atención a alcohólicos, ubicados en la provincia de Córdoba, España.ParticipantesSeiscientos catorce pacientes con edades de entre 18 y 80 años.Mediciones principalesMediante entrevista personal se realizaron los cuestionarios MALT, AUDIT (Alcohol Use Disorders Identification Test) y el SCAN (Schedules for clinical assessment in Neuropsychiatry). Se analizó la fiabilidad interna, la reproducibilidad, la validez criterial (patrón de referencia: criterios del Manual Diagnóstico y Estadístico de los Trastornos Mentales IV [DSM-IV]) y la validez convergente.ResultadosLa edad media de la población fue de 43 años (desviación estándar de 1,43). El 17,4% presentó síndrome de dependencia alcohólica. Los coeficientes kappa de los ítems del test oscilaban entre 0,568 y 0,969. El alfa de Cronbach del MALT completo fue de 0,919; el alfa de Cronbach del MALT subjetivo fue de 0,939, y el alfa de Cronbach del MALT objetivo fue de 0,737. El área bajo la curva de eficacia diagnóstica para el MALT-S fue de 0,946 y para el MALT completo fue de 0,953. Para un punto de corte de 4, la sensibilidad del MALT-S fue del 92,6% y la especificidad fue del 90,8%. El coeficiente de correlación del MALT-S y del AUDIT fue de 0,86 (p<0,001).ConclusionesEl MALT subjetivo es un cuestionario tan válido y fiable como el MALT completo, por lo que puede ser utilizado aisladamente para la detección y el diagnóstico de alcoholismo.AbstractObjectiveTo validate the subjective part of the MALT questionnaire (MALT-S) for the detection of alcoholism among the adult population.DesignA descriptive study on the validation of a diagnostic tool.SettingTwo primary health care centres, a centre for drug addicts and a centre for alcoholics in Cordoba (Spain).Participants614 patients, between 18 and 80 years.MeasurementsThrough personal interviews the MALT, AUDIT and SCAN questionnaires were given. The internal reliability, stability and both the criterion and convergent validity were analysed.ResultsThe mean age of the population’ was 43±1.43 (SD) years and 17.4% of them showed Alcohol Dependence Syndrome. The Kappa coefficients of the test items ranged from 0.568 to 0.969. The Cronbach alpha of the complete MALT was 0.919, of the MALT-S, 0.939 and of the MALT-O, 0.737. The area under the ROC curve of the MALT-S was 0.946 and that of the complete MALT was 0.953. For a cut-off point of four, the sensitivity of the MALT-S is 92.7% while its specificity was 90.9%. The correlation coefficient of the MALT-S and AUDIT was 0.86 (P<0.001).ConclusionsThe MALT-S questionnaire is as valid and reliable as the complete MALT; therefore, it can be used on its own for the diagnosis of alcoholism

Graphene derivative scaffolds facilitate in vitro cell survival and maturation of dopaminergic SN4741 cells

The emerging carbon nanomaterial Graphene (G), in the form of scaffold structure, has an efficient bioconjugation with common biomolecules and activates cell differentiation of neuronal stem cells, providing a promising approach for neural regeneration. We propose the use of G as a scaffold to re-address the dopaminergic (DA) neurons and the residual axons from dead or apoptotic DA neurons in Parkinson´s disease (PD). G could act as a physical support to promote the axonal sprout as a “deceleration” support for the DA cells derived from neural stem cells or DA direct cell conversion, allowing the propagation of nerve impulses. We cultured a clonal substantia nigra (SN) DA neuronal progenitor cell line (SN4741) in presence of G as scaffold. This cell line derived from mouse embryos was cultured in Dulbecco’s modified Eagle’s medium/10% FCS to about 80% confluence. Cells were incubated in three chemically different G derivatives and two different presentation matrixes as powder and films: 1) G oxide (GO); 2) partially reduced GO (PRGO) which is hydrophobic; and 3) fully reduced GO (FRGO). Cell viability was determined using the MTT assay after adding the following G concentrations: 1mg/ml; 0.1mg/ml; 0.05mg/ml; 0.02mg/ml and 0.01mg/ml, in each type of GO. To study cellular morphology and assessment of cell engraftment into GO films (GO film, PRGO film, FRGO film), we analyzed the immunostaining of the anti-rabbit neuron-specific DNA-binding protein (NeuN) antibody, the anti-rat Beta-3-tubulin antibody in combination with the mitochondrial marker mouse anti-ATP synthase antibody, and the anti-rabbit DCX as immature neuronal marker. Hoechst label was used as nuclei marker. Reactive oxidative species (ROS) were measured by flow cytometry to study the influence of G on the cell redox-state. With this purpose, cells were loaded with dihydroethidium. The mitochondrial membrane potential after JC-1 incubation was studied by flow cytometry. Our results show an increase of survival and metabolism (30-40%) at low concentrations of PRGO and FRGO (0.05-0.01 mg/ml) respect to the higher concentration (1 mg/ml), while no changes were seen in the GO group. LDH concentration was measured in the supernatant using a COBAS analyzer showing a neuroprotective action at low concentrations. Furthermore, either PRGO film or FRGO film show an increase in the effective anchorage capacity to nest into the G matrix and in the maturation of the SN 4741 cells. We conclude that the use of G scaffolds in the research of neurological diseases like PD could offer a powerful platform for neural stem cells, direct cell conversion techniques and neural tissue engineering.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Norwegian Research Council (grant nº 215086

Benchmarking of DFTmethods using experimental free energies and volumes of activation for the cycloaddition of alkynes to cuboidalMo(3)S(4)clusters

Here, the kinetics of the concerted [3 + 2] cycloaddition reaction between the [Mo3(μ3‐S)(μ‐S)3Cl3(dmen)3]+ (dmen = N,N′‐dimethyl‐ethylenediamine) ([1]+) cluster and various alkynes to form dithiolene derivatives is thoroughly studied, with measurements at different temperatures and pressures allowing the determination of the free energies and volumes of activation. These parameters, together with the available single‐crystal X‐ray diffraction structures, are used to test a number of commonly used density functional theory (DFT) methods from Jacob's ladder, as well as the effects associated with the size of the basis sets, the way in which solvent effects are taken into account, or the inclusion of dispersion effects. Overall, a protocol that leads to average deviations between experimental and computed ΔV‡ and ΔG‡ values similar to the uncertainty of the experimental measurements is obtained

Comparison of Three-phase Active Rectifier Solutions for Avionic Applications: Impact of the Avionic Standard DO-160 F and Failure Modes

In aircraft applications, there has been an increasing trend related with the More Electric Aircraft (MEA), which results in rapid rise in the electrical power demand on-board. One of its goals lies in minimizing weight and volume of the electrical subsystem while maintaining good power quality and efficiency. The main purpose of this paper is to present and analyze an electrical design of a three-phase Boost rectifier, a three-phase Buck rectifier and a three-phase Vienna rectifier for output power level of 10 kW and compare them in terms of weight, volume, efficiency etc. Moreover, the design is obliged to comply with specific sections of DO-160 standard for avionic equipment with 230 VAC, 360-800 Hz grid conditions. Even though all proposed solutions satisfy the standard requirements, it will be shown that the Vienna rectifier has the lowest volume and not considering failure modes, the better solution overall. However, due to increased number of semiconductors and additional circuitry required for soft start-up, the Buck rectifier would prove to be the more robust solution failure-wise

Cytokinin Determines Thiol-Mediated Arsenic Tolerance and Accumulation

The presence of arsenic in soil and water is a constant threat to plant growth in many regions of the world. Phytohormones act in the integration of growth control and stress response, but their role in plant responses to arsenic remains to be elucidated. Here, we show that arsenate [As(V)], the most prevalent arsenic chemical species in nature, causes severe depletion of endogenous cytokinins (CKs) in the model plant Arabidopsis (Arabidopsis thaliana). We found that CK signaling mutants and transgenic plants with reduced endogenous CK levels showed an As(V)-tolerant phenotype. Our data indicate that in CK-depleted plants exposed to As(V), transcript levels of As(V)/phosphate-transporters were similar or even higher than in wild-type plants. In contrast, CK depletion provoked the coordinated activation of As(V) tolerance mechanisms, leading to the accumulation of thiol compounds such as phytochelatins and glutathione, which are essential for arsenic sequestration. Transgenic CK-deficient Arabidopsis and tobacco lines show a marked increase in arsenic accumulation. Our findings indicate that CK is an important regulatory factor in plant adaptation to arsenic stress

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Effect of sex in systemic psoriasis therapy: Differences in prescription, effectiveness and safety in the BIOBADADERM prospective cohort

The effect of sex on systemic therapy for psoriasis has not been well studied. The aim of this study was to analyse a large multicentre Spanish cohort of 2,881 patients with psoriasis (58.3% males), followed from January 2008 to November 2018, to determine whether sex influences prescription, effectiveness of therapy, and the risk of adverse events. The results show that women are more likely than men to be pre-scribed biologics. There were no differences between men and women in effectiveness of therapy, measur-ed in terms of drug survival. Women were more likely to develop adverse events, but the difference in risk was small and does not justify different management. Study limitations include residual confounding and the use of drug survival as a proxy for effectiveness.The BIOBADADERM project is promoted by the Fundación Piel Sana Academia Española de Dermatología y Venereología, which receives financial support from the Spanish Medicines and Health Products Agency (Agencia Española de Medicamentos y Productos Sanitarios) and from pharmaceutical companies (Abbott/Abbvie, Pfizer, MSD, Novartis, Lilly, Janssen and Almirall)