TO EVALUATE THE EFFECT OF SURFACE MOISTURE ON DENTINAL SHEAR BOND STRENGTH OF GLASS IONOMER RESTORATIONS IN PRIMARY TEETH:AN IN -VITRO STUDY

Glass ionomer cements that form aninevitable part of pediatric restorative dentistryare inherently sensitive to moisture. The influenceof different drying techniques on the shear bondstrength of glass ionomer cements to primaryteeth dentin has not been established.Thepurpose of the study was to evaluate the effects ofdifferent drying methods for different drying timeperiods on the shear bond strength of GC Fuji IX toprimary tooth dentine

Publication trends in journal of clinical and experimental dentistry

Journal of Clinical and Experimental Dentistry (J Clin Exp Dent; JCED) is an English language journal published by the Spanish Society of Oral Surgery, and has been online since 2009. It is indexed in PubMed Central and Scopus since 2012, with monthly publications since 2016. The purpose of this article was to review and analyse the publications in this journal since its inception, over a period of 11 years (2009-2019). This paper assessed the number, type and subjects of the articles published in the journal over 11 years. The institutions of the first authors, number of PubMed citations and the Hirsch (h5) index was assessed and analysed. The manuscripts published in JCED have gradually increased over the years, with Original research articles accounting for the bulk of contributions. The journal publishes articles mainly from the subjects of Oral Pathology and Operative Dentistry and Endodontics. Articles published in JCED are indexed in PubMed Central (since 2012), Scopus, DOI system, and Google Scholar. A country-wise mapping of the (first) author?s institutions revealed significant contributions from researchers from all over the world. With an h5 index of 26, the journal was ranked among the top six multispeciality journals. The most cited articles were the literature reviews on common oral lesions (recurrent apthous stomatitis and candidiasis). The journal has contributed to the growth of scientific literature pertaining to subjects from all the fields of dentistry. Over the past 11 years, JCED has served as a platform for large number of manuscripts in all the disciples of dentistry, from researches all over the world

Elucidation of the molecular responses during the primary infection of wild blueberry phenotypes with Monilinia vaccinii-corymbosi under field conditions

Background - Monilinia blight caused by Monilinia vaccinii-corymbosi (Reade) Honey (M.vc) is a major disease of wild blueberry that can result in severe crop losses in the absence of an integrated disease management programme. The fungus causes blight in the emerging floral and vegetative buds, but the degree of susceptibility varies among the different wild blueberry phenotypes, ranging from the highly susceptible V. a. f. nigrum to the moderately susceptible V. angustifolium and the least susceptible V. myrtilloides. Results - The present study evaluated the defense responses of these major phenotypes during their primary infection (floral buds) with M.vc. The temporal expression profiles of PR genes (PR3 and PR4) and the flavonoid pathway structural genes (CHS, ANS, ANR, DFR and FLS) were analysed. The PR3 and PR4 gene expression profiles revealed that V. myrtilloides responded to M.vc infection by activating the expression of both PR genes. V. a. f. nigrum, on the other hand, failed to activate these genes, while V. angustifolium, exhibited an intermediate response. Our study with the flavonoid pathway genes indicated variability in activation of the genes during post-infection time points with ANS and ANR in V. myrtilloides, FLS in V. angustifolium and no response observed in V. a. f. nigrum. Conclusions - Altogether, this study highlights that the degree of phenotype susceptibility is associated with the timely activation of host defense responsive genes. Data obtained in this study provided a starting point for a better understanding of the wild blueberry- M. vaccinii-corymbosi pathosystem

\u3cem\u3eMonilinia vaccinii-corymbosi\u3c/em\u3e sensitivity to demethylation inhibitor fungicides and its effect on Monilinia blight control in wild blueberry fields

Monilinia vaccinii-corymbosi (Reade) Honey (M.vc), the causal agent of Monilinia blight of wild blueberry, is controlled primarily by fungicide applications. Demethylation-inhibiting fungicides (DMIs) have been used for over 30 years for Monilinia blight control due to flexibility of use (i.e., ability to use after an infection period) and disease control effectiveness and consistency. In the present study, the sensitivity of ten M.vc isolates to three DMIs- propiconazole, difenoconazole and prothioconazole-desthio were evaluated in vitro by a mycelial growth inhibition assay. In addition, four field trials were conducted during two crop seasons: 2012 and 2013, to examine the efficacy of these DMIs to control Monilinia blight. All the tested DMIs were effective in inhibiting mycelial growth of M.vc isolates, although the mean EC50 values differed significantly. In field experiments, three of four trials had significant treatment effect on disease incidence and severity of vegetative buds. Prothioconazole-desthio and propiconazole provided consistent control against Monilinia blight. Conversely, difenoconazole was effective in in vitro analysis, but did not demonstrate satisfactory Monilinia blight control in all field trials. In the 2012 trials, both prothioconazole-desthio and propiconazole reduced disease incidence of vegetative buds by 100% compared to the untreated control. Prothioconazole- desthio reduced disease development in 2013 with 94 and 99.8% less incidence, and 75 and 99.5% less severity. Similarly, propiconazole also reduced incidence of vegetative buds by 88% and 99.8%, and severity by 54% and 99.7%. No phytotoxic symptoms were observed in any of the field trials. The results of the study serve as a benchmark to monitor shifts in M.vc sensitivity to these fungicides in the future

Identification of variety-specific ISSR markers in small cardamom (Elettaria cardamomum Maton.)

----------

Diversity analysis of released varieties of Indian cardamom using ISSR markers reveal narrowing genetic base

311-322Elettaria cardamomum Maton the small cardamom of commerce is a monotypic genus in India under the family Zingiberaceae. Genetic diversity studies using inter simple sequence repeat (ISSR) markers were conducted on a total of 13 released varieties of cardamom including selections and hybrids from different research stations in South India along with a popular variety Njallani and a wild relative Aframomum sps as checks. ISSR markers however, revealed relatively high level of genetic redundancy among the tested varieties with the exception of a few. Nonetheless, unweighted pair group method of arithmetic average (UPGMA) based cluster analysis of the similarity matrix differentiated all the varieties tested with the exception of two and segregated the wild relative Aframomum. Considerable reduction of polymorphism percentage was observed on exclusion of Aframomum while studying ISSR polymorphism which could be an indicator of the narrowing genetic base in the released varieties. Morphological data were compiled based on International Plant Genetic Resources Institute (IPGRI) cardamom descriptor and revealed moderate variability among the varieties. The results highlighted the requirement of molecular characterization of unutilized germplasm accessions, related wild species, and exotic relatives using specific molecular markers to help refine breeding efforts and introgression of new alleles for further improvement and enhancement of genetic base of cultivated cardamom

Selection and validation of reliable reference genes for gene expression studies from Monilinia vaccinii-corymbosi infected wild blueberry phenotypes

Monilinia blight disease caused by Monilinia vaccinii-corymbosi (Reade) Honey (M.vc) causes severe damage and economic losses in wild blueberry growing regions. Molecular mechanisms regulating defence responses of wild blueberry phenotypes towards this causal fungus are not yet fully known. A reliable quantification of gene expression using quantitative real time PCR (qPCR) is fundamental for measuring changes in target gene expression. A crucial aspect of accurate normalisation is the choice of appropriate reference genes. This study evaluated the expression stability of seven candidate reference genes (GAPDH, UBC9, UBC28, TIP41, CaCSa, PPR and RH8) in floral tissues of diploid and tetraploid wild blueberry phenotypes challenged with M.vc. The expression stability was calculated using five algorithms: geNorm, NormFinder, BestKeeper, deltaCt and RefFinder. The results indicated that UBC9 and GAPDH were the most stable reference genes, while RH8 and PPR were the least stable ones. To further validate the suitability of the analyzed reference genes, the expression level of a pathogenesis related protein gene (i.e., PR3) was analysed for both phenotypes at four time points of infection. Our results may be beneficial for future studies involving the quantification of relative gene expression levels in wild blueberry species.publishedVersio

Modulation of defense genes and phenolic compounds in wild blueberry in response to Botrytis cinerea under field conditions

Botrytis blight is an important disease of wild blueberry [(Vaccinium angustifolium (Va) and V. myrtilloides (Vm))] with variable symptoms in the field due to differences in susceptibility among blueberry phenotypes. Representative blueberry plants of varying phenotypes were inoculated with spores of B. cinerea . The relative expression of pathogenesis-related genes (PR3, PR4), flavonoid biosynthesis genes, and estimation of the concentration of ten phenolic compounds between uninoculated and inoculated samples at different time points were analyzed. Representative plants of six phenotypes (brown stem Va , green stem Va , Va f. nigrum, tall, medium, and short stems of Vm ) were collected and studied using qRT-PCR. The expression of targeted genes indicated a response of inoculated plants to B. cinerea at either 12, 24, 48 or 96 h post inoculation (hpi). The maximum expression of PR3 occurred at 24 hpi in all the phenotypes except Va f. nigrum and tall stem Va . Maximum expression of both PR genes occurred at 12 hpi in Va f. nigrum. Chalcone synthase, flavonol synthase and anthocyanin synthase were suppressed at 12 hpi followed by an upregulation at 24 hpi. The expression of flavonoid pathway genes was phenotype-specific with their regulation patterns showing temporal differences among the phenotypes. Phenolic compound accumulation was temporally regulated at different post-inoculation time points. M-coumaric acid and kaempferol-3-glucoside are the compounds that were increased with B. cinerea inoculation. Results from this study suggest that the expression of PR and flavonoid genes, and the accumulation of phenolic compounds associated with B. cinerea infection could be phenotype specific. This study may provide a starting point for understanding and determining the mechanisms governing the wild blueberry- B. cinerea pathosystem

Electric Vehicle Air Conditioning System and Its Optimization for Extended Range—A Review

Environmental protection initiatives are speeding up the replacement of the present IC engine-based transportation system with an electric motor-driven system. In electric vehicles (EV), energy stored in batteries is used for the traction of the vehicle and the operation of the auxiliaries. The range of the electric vehicle was identified to be one of the major challenges faced by the EV segment. The optimization of the consumption of stored energy is the best solution for range improvement in an EV. Auxiliaries in the vehicle include basic accessories such as a lighting system and equipment for improved comfort such as air conditioners. Air conditioning equipment is the major power-consuming auxiliary in an EV apart from the traction motor. This review article discusses the significance and influence of different components of the air conditioning system, and methods followed by researchers to optimize the performance and reduce the energy consumption of the air conditioning system to extend the range of vehicles. The effects of thermal load and volume of space to be conditioned were also considered in this study. This review concludes by stating the different possibilities for the reduction in power consumption and emphasizes zonal air conditioning of occupant space as a solution for reducing energy consumption or increasing the range of EVs. Compared to full-space air conditioning, zonal AC can reduce power consumption by up to 51%