Conception, development and validation of a software interface to assess human’s horizontal intra-cyclic velocity with a mechanical speedo-meter

The aim of this paper was to: (i) calibrate the Swimsportec® speedo-meter; (ii) concept and develop a software’s interface for it (iii) validate the full system. Calibration was done with an industrial robot. The robot arm’s performed a linear and horizontal path at a uniform movement for a set of wide range of velocities (0.5<v<4.5m/s) being the tension data acquire for each specific linear velocity. Software’s interface was developed in LabVIEW® to acquire, display and process pair wises velocity-time data on-line during the subject’s locomotion bout. To transfer data from speedo-meter to the software an acquisition card is used. System validation was done for a set of land-based human locomotion techniques (from slow walk to maximal running) in four subjects and comparing it with a Doppler radar gun. Linear regression models between speedo-meter system and radar gun where very high for both the coefficient of variation of the subject’s velocity within the full gait cycle and his maximal velocity. The 95% of interval confidence agreement limits were very close together in both variables. More than 80% of the Bland- Altman plots were with the 1.96 standard-deviation criteria used on regular basis as rule thumb for techniques validation

Tracking the performance of world-ranked swimmers

Tracking the swimming performance is important to analyze its progression and stability between competitions and help coaches to define realistic goals and to select appropriate training methods. The aim of this study was to track world-ranked male swimmer’s performance during five consecutive seasons (from 2003/2004 to 2007/2008) in Olympic freestyle events. An overall of 477 swimmers and 2385 season best performances were analyzed. FINA’s male top-150 rankings for long course in the 2007-2008 season were consulted in each event to identify the swimmers included. Best performances were collected from ranking tables provided by the National Swimming Federations or, when appropriate, through an internet database (www.swimranking.net). Longitudinal assessment was performed based on two approaches: (i) mean stability (descriptive statistics and ANOVA repeated measures, followed by a Bonferroni post-hoc test) and; (ii) normative stability (Pearson Correlation Coefficient and the Cohen’s Kappa tracking index). Significant variations in the mean swimming performance were observed in all events between all seasons. Performance enhancement was approximately 0.6 to 1 % between seasons leading up to the Olympics and approximately 3 to 4 % for the overall time-frame analyzed. The performance stability based on overall time-frame was moderate for all freestyle events, except in the 50-m (K = 0.39 ± 0.05) where it was low. Selfcorrelations ranged between a moderate (0.30 ≤ r < 0.60) and a high (r ≥ 0.60) stability. There was also a performance enhancement during all five seasons analyzed. When more strict time frames were used, the analysis of swimming performance stability revealed an increase in the third season. So, coaches should have a long term view in what concerns training design and periodization of world-ranked swimmers, setting the third season of the Olympic Cycle as a determinant time frame, due to performance stability until Olympic Games season

Infection with carcinogenic helminth parasites and its production of metabolites induces the formation of DNA-adducts

Background: Infections classified as group 1 biological carcinogens include the helminthiases caused by Schistosoma haematobium and Opisthorchis viverrini. The molecular mediators underlying the infection with these parasites and cancer remain unclear. Although carcinogenesis is a multistep process, we have postulated that these parasites release metabolites including oxysterols and estrogen-like metabolites that interact with host cell DNA. How and why the parasite produce/excrete these metabolites remain unclear. A gene encoding a CYP enzyme was identified in schistosomes and opisthorchiids. Therefore, it is reasonable hypothesized that CYP 450 might play a role in generation of pro-inflammatory and potentially carcinogenic compounds produced by helminth parasites such as oxysterols and catechol estrogens. Here, we performed enzymatic assays using several isoforms of CYP 450 as CYP1A1, 2E1 and 3A4 which are involved in the metabolism of chemical carcinogens that have been associated with several cancer. The main aim was the analysis of the role of these enzymes in production of helminth-associated metabolites and DNA-adducts. Method: The effect of cytochrome P450 enzymes CYP 1A1, 2E1 and 3A4 during the interaction between DNA, glycocholic acid and taurochenodeoxycholate sodium on the formation of DNA-adducts and metabolites associated with urogenital schistosomiasis (UGS) and opisthorchiasis was investigated in vitro. Liquid chromatography/mass spectrometry was used to detect and identify metabolites. Main findings: Through the enzymatic assays we provide a deeper understanding of how metabolites derived from helminths are formed and the influence of CYP 450. The assays using compounds similar to those previously observed in helminths as glycocholic acid and taurochenodeoxycholate sodium, allowed the detection of metabolites in their oxidized form and their with DNA. Remarkably, these metabolites were previously associated with schistosomiaisis and opisthorchiasis. Thus, in the future, it may be possible to synthesize this type of metabolites through this methodology and use them in cell lines to clarify the carcinogenesis process associated with these diseases. Principal conclusions: Metabolites similar to those detected in helminths are able to interact with DNA in vitro leading to the formation of DNA adducts. These evidences supported the previous postulate that imply helminth-like metabolites as initiators of helminthiases-associated carcinogenesis. Nonetheless, studies including these kinds of metabolites and cell lines in order to evaluate its potential carcinogenic are required.This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia, in the framework of the project, Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274). The FCT and FEDER (European Union) also supported these studies through project number IF/00092/2014/CP1255/CT0004info:eu-repo/semantics/publishedVersio

The influence of ACE ID and ACTN3 R577X polymorphisms on lower-extremity function in older women in response to high-speed power training

Background We studied the influence of the ACE I/D and ACTN3 R577X polymorphisms (single or combined) on lower-extremity function in older women in response to high-speed power training. Methods One hundred and thirty-nine healthy older Caucasian women participated in this study (age: 65.5 ± 8.2 years, body mass: 67.0 ± 10.0 kg and height: 1.57 ± 0.06 m). Walking speed (S10) performance and functional capacity assessed by the “get-up and go” (GUG) mobility test were measured at baseline (T1) and after a consecutive 12-week period of high-speed power training (40-75% of one repetition maximum in arm and leg extensor exercises; 3 sets 4–12 reps, and two power exercises for upper and lower extremity). Genomic DNA was extracted from blood samples, and genotyping analyses were performed by PCR methods. Genotype distributions between groups were compared by Chi-Square test and the gains in physical performance were analyzed by two-way, repeated-measures ANOVA. Results There were no significant differences between genotype groups in men or women for adjusted baseline phenotypes (P > 0.05). ACE I/D and ACTN3 polymorphisms showed a significant interaction genotype-training only in S10 (P = 0.012 and P = 0.044, respectively) and not in the GUG test (P = 0.311 and P = 0.477, respectively). Analyses of the combined effects between genotypes showed no other significant differences in all phenotypes (P < 0.05) at baseline. However, in response to high-speed power training, a significant interaction on walking speed (P = 0.048) was observed between the “power” (ACTN3 RR + RX & ACE DD) versus “non-power” muscularity-oriented genotypes (ACTN3 XX & ACE II + ID)]. Conclusions Thus, ACE I/D and ACTN3 R577X polymorphisms are likely candidates in the modulation of exercise-related gait speed phenotype in older women but not a significant influence in mobility traits

Nutritional and Health Profile of Goat Products: Focus on Health Benefits of Goat Milk

Goat (Capra hircus) is one of the main sources of milk and meat products for human consumption. Goat milk differs from cow and human milk in both composition and nutritional properties. Goat milk and other goat-derived products contain several bioactive compounds that might be useful in patients suffering from a variety of chronic diseases. Several peptides, fats, and oligosaccharides present in goat’s milk can be potentially useful in cardiovascular disease, metabolic disorders, neurological degeneration, or in promoting intestinal health. They have also shown chemopreventive properties in cancer. In addition, the oligosaccharides present in goat’s milk have immunomodulatory properties, prevent adhesion of pathogenic bacteria, and have prebiotic, probifidogenic effects. Due to its potential health benefits, goat milk is particularly recommended for infants, older adults, and convalescing people. This chapter gives an overview of the biological activities of goat products and the effects of peptides, fats, and oligosaccharides present in goat milk on pathogenic bacteria, as well as their ability to regulate immunological, gastrointestinal, hormonal, and neurological responses in humans

Human-robot collaboration (HRC) with vision inspection for PCB assembly

Flexibility and speed in the development of new industrial machines are essential factors for the success of capital goods industries. When assembling a printed circuit board (PCB), since all the components are surface-mounted devices (SMD), the whole process is automatic. However, in many PCBs, it is necessary to place components that are not SMDs, called pin through-hole components (PTH), having to be inserted manually, which leads to delays in the production line. This work proposes and validates a prototype work cell based on a collaborative robot and vision systems whose objective is to insert these components in a completely autonomous or semi-autonomous way. Different tests were made to validate this work cell, showing the correct implementation and the possibility of replacing the human worker on this PCB assembly task.ERDF - European Regional Development Fund(45070

Population overlap and habitat segregation in wintering Black-tailed Godwits Limosa limosa

Distinct breeding populations of migratory species may overlap both spatially and temporally, but differ in patterns of habitat use. This has important implications for population monitoring and conservation. To quantify the extent to which two distinct breeding populations of a migratory shorebird, the Black-tailed Godwit Limosa limosa, overlap spatially, temporally and in their use of different habitats during winter. We use mid-winter counts between 1990 and 2001 to identify the most important sites in Iberia for Black-tailed Godwits. Monthly surveys of estuarine mudflats and rice-fields at one major site, the Tejo estuary in Portugal in 2005-2007, together with detailed tracking of colour-ringed individuals, are used to explore patterns of habitat use and segregation of the Icelandic subspecies L. l. islandica and the nominate continental subspecies L. l. limosa. In the period 1990-2001, over 66 000 Black-tailed Godwits were counted on average in Iberia during mid-winter (January), of which 80% occurred at just four sites: Tejo and Sado lower basins in Portugal, and Coto Dontildeana and Ebro Delta in Spain. Icelandic Black-tailed Godwits are present throughout the winter and forage primarily in estuarine habitats. Continental Black-tailed Godwits are present from December to March and primarily use rice-fields. Iberia supports about 30% of the Icelandic population in winter and most of the continental population during spring passage. While the Icelandic population is currently increasing, the continental population is declining rapidly. Although the estuarine habitats used by Icelandic godwits are largely protected as Natura 2000 sites, the habitat segregation means that conservation actions for the decreasing numbers of continental godwits should focus on protection of rice-fields and re-establishment of freshwater wetlands

Vertical variability of trophic positions of zooplankton in the deep Ocean

Zooplankton plays a key role in oceanic ecosystems. However, the trophic ecology of organisms in deep layers of the ocean is poorly known. In this study we analyze the variability of trophic positions of zooplankton collected across three ocean basins in the epi-, meso and bathypelagic domains. Stable carbon and nitrogen isotopes were used as indicators of the sources of nutrients and positions within the food web. The enrichment in heavy nitrogen isotopes with depth and the correlation between surface and deep samples revealed that deep zooplankton was supported by local epipelagic production, subsequently processed through the water column. In addition the nitrogen isotope enrichment of carnivores vs. omnivores was consistent across ocean biomes and water layers, suggesting a similar trophic structure of the pelagic food web in the deep ocean despite variations in the nitrogen sources. Siphonofora, Chaetognata and Myctophida were the top predators while Calanoid Copepoda and Mysidacea displayed the lowest trophic positions. In contrast, carbon isotopes did not show significant variations with depth or trophic groups implying low influence of coastal production in deep ocean food webs