53 research outputs found
Brazilian Network for HIV Drug Resistance Surveillance: a survey of individuals recently diagnosed with HIV
Use of antiretrovirals is widespread in Brazil, where more than 200,000 individuals are under treatment. Although general prevalence of primary antiretroviral resistance in Brazil is low, systematic sampling in large metropolitan areas has not being performed
Polymorphism of the ACE Gene is Associated with Extent and Severity of Coronary Disease
Introdução: Os doentes com doença das
artérias coronárias (DAC) apresentam
extensão da doença e evolução muito
variáveis, que muito vezes nos escapam e que
ultrapassam os factores de risco tradicionais.
As diferenças poderão, pelo menos em parte,
ser explicáveis por polimorfismos genéticos
menos favoráveis que lhe estejam associados.
Os polimorfismos do gene da ECA têm sido
profusamente avaliados, embora se
desconheça a ligação entre estes
polimorfismos e a extensão da DAC.
Objectivo: Os autores pretendem avaliar se os
polimorfismos do gene da enzima de conver são da Angiotensina I (ECA) constituem um
marcador da extensão e gravidade da DAC.
Métodos: Estudo descritivo, em 296 doentes
com história de enfarte do miocárdio ou
doença coronária confirmada por
coronariografia, com pelo menos 75 % de
obstrução de um dos vasos coronários.
A quantificação da gravidade e extensão, foi
feita segundo o score de Leaman, de acordo
com o número de artérias com redução do
diâmetro superior a 75 %, e com o número de
segmentos coronários afectados.
Os genotipos do ECA, foram tipados por
amplificação por PCR e os produtos de
amplificação separados por electroforese em
gel de poliacrilamida. Calculou-se a média e desvio padrão dos
scores coronários dos três polimorfismos e os
valores foram comparados estatisticamente
recorrendo ao teste T de Student para
amostras independentes.
Resultados e Conclusão: O genotipo DD
aparece neste estudo claramente ligado à
extensão da DAC, com um alto grau de
significância. A confirmar-se este conceito,
poderá justificar-se fazer uma prevenção
secundária particularmente cuidadosa nos
doentes vasculares portadores deste genotipo.Background: The progression and extent of
coronary heart disease (CHD) are extremely
variable and in many instances independent
of conventional risk factors.
The differences may be partly explained by
less favorable genetic polymorphisms that are
associated with them. The polymorphisms of
the angiotensin I converting enzyme (ACE)
gene have been thoroughly evaluated, but the
connection between them and the extent of
CHD is unknown.
Aims: Our study is aimed at determining
whether any or all of the polymorphisms of
the ACE gene are markers of the extent and
severity of CHD.
Methods: This was a descriptive study of 296
patients with a history of myocardial
infarction or with coronary disease confirmed
by coronary angiography. The severity of
CHD was quantified according to Leaman’s
score (based on the number of arteries with
more than 75 % reduction in diameter and the
number of affected coronary segments). The
ACE genotypes were determined by specific
polymerase chain reaction amplification and
the segments were subjected to
polyacrylamide gel electrophoresis. The mean coronary score and standard deviation of the
three polymorphisms were calculated and the
values statistically compared using the
Student’s t test for independent samples.
Results: 296 patients with a mean age of
5510.3 years, 234 male, were evaluated.
Conclusion: The study clearly shows that the
DD genotype is linked to the extent of CHD,
with a high level of significance. If this is
confirmed, careful secondary prevention is
indicated in patients with this genotype.info:eu-repo/semantics/publishedVersio
Angiotensin Converting Enzyme Gene Polymorphisms and Coronary Risk in a Portuguese Population
Introdução: A história familiar de doença das
artérias coronárias (DAC) constitui um
poderoso marcador de risco de DAC,
independente dos factores de risco
tradicionais. Poderá ser descodificado
reconhecendo os polimorfismos associados ao
aumento de risco. Têm surgido resultados
contraditórios em relação à ligação entre os
polimorfismos do gene da enzima de conver são da angiotensina (ECA) e o risco de DAC.
Objectivo: Com o presente trabalho
pretendemos avaliar se os polimorfismos do
gene da ECA constituem factor de risco de
doença das artérias coronárias.
Métodos: Estudo caso-controlo, incluindo
517 controlos escolhidos aleatoriamente dos
cadernos eleitorais, sem história sugestiva de
DAC e 301 doentes com história de enfarte
agudo do miocárdio ou doença coronária
confirmada por coronariografia, com pelo
menos 75 % de obstrução de um dos vasos
coronários. Tentou-se que os casos e controlos
não fossem significativamente diferentes em
termos de sexo e idade.
Os polimorfismos dialélicos do gene da ECA
foram tipados por amplificação por PCR. Os
produtos de amplificação eram identificados
em gel de poliacrilamida, por electroforese.
Os dados foram avaliados recorrendo ao SPSS
for Windows,Background: A family history of coronary
heart disease (CHD) is a strong risk marker
for the disease, independently of classical
risk factors. It could be decoded by
recognizing the polymorphisms associated
with increased risk. Renin-angiotensin
system genes are candidate genes in CHD
and the deletion allele of the angiotensin
converting enzyme (ACE) has been reported
as deleterious. However, there is
disagreement as to the role of the
insertion/deletion polymorphism of the ACE
gene in coronary risk.
Aim: To evaluate whether ACE gene
polymorphisms constitute a CHD risk factor.
Methods: We conducted a population-based
case-control study of 301 subjects with a
history of myocardial infarction or
angiographic evidence of coronary heart
disease and 510 age- and gender-matched
controls, without CHD, living in a region with
high CHD mortality rates. Blood samples
were taken, DNA extracted and genotypes
determined by the polymerase chain reaction
(PCR). Amplification products were identified
by agarose gel electrophoresis.info:eu-repo/semantics/publishedVersio
Exclusion of mutations in the PRNP, JPH3, TBP, ATN1, CREBBP, POU3F2 and FTL genes as a cause of disease in Portuguese patients with a Huntington-like phenotype
Huntington disease (HD) is an autosomal dominant neurodegenerative disorder characterised by chorea, cognitive impairment, dementia and personality changes, caused by the expansion of a CAG repeat in the HD gene. Often, patients with a similar clinical presentation do not carry expansions of the CAG repeat in this gene [Huntington disease-like (HDL) patients]. We report the genetic analysis of 107 Portuguese patients with an HDL phenotype. The HDL genes PRNP and JPH3, encoding the prion protein and junctophilin-3, respectively, were screened for repeat expansions in these patients. Given the partial clinical overlap of SCA17, DRPLA and neuroferritinopathy with HD, their causative genes (TBP, ATN1, and FTL, respectively) were also analysed. Finally, repeat expansions in two candidate genes, CREBBP and POU3F2, which encode the nuclear transcriptional coactivator CREB-binding protein and the CNS-specific transcription factor N-Oct-3, respectively, were also studied. Expansions of the repetitive tracts of the PRNP, JPH3, TBP, ATN1, CREBBP and POU3F2 genes were excluded in all patients, as were sequence alterations in the FTL gene. Since none of the genes already included in the differential diagnosis of HD was responsible for the disease in our sample, the genetic heterogeneity of the HDL phenotype is still open for investigation.Fundação para a Ciência e a Tecnologia (FCT) and FEDER (grant CBO/33485/99). BIC included in grant CBO/33485/99, respectivel
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing
Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management.
Results: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org.
Conclusions: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.Peer Reviewe
“Se viveres como louco, sabes que hás de morrer sem juízo”: as orientações para o bem morrer na literatura cristã portuguesa do século XVIII
Resumo Orientar os fiéis para uma vida santificada e instruí-los para uma boa morte foram objetivos muito comuns na literatura religiosa portuguesa do período moderno, especialmente aquela divulgada no século XVIII e que se propunha a definir e propagar virtudes morais e comportamentos que garantissem o bem morrer. Nosso objetivo, neste artigo, é o de analisar as orientações que todo fiel católico deveria observar como preparação prévia para a morte nas obras Sermão da Missão da quarta tarde da quaresma (1734), Terceiro Instruído na Virtude (1742) e Mestre da Virtude(1745), produzidas pelo padre dominicano português João Franc
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