3,057 research outputs found

    Field Application of a Monoclonal Antibody Panel to Differentiate Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Isolates

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    Porcine reproductive and respiratory syndrome (PRRS) is recognized as the most economically devastating disease of swine throughout the world. Nucleotide sequencing and serological studies have deomnstrated sustantial antigenic variation amoung PRRS virus (PRRSV) isolates (Murtaugh, et al., 1995; Nelson, et al., 1993, 1996). Recently, European-like PRRSV isolates have been identified in the US (Rossow, et al. 2000) and other new, highly virulent PRRSV isolates have appeared and affect sows at all stages of gestation causing mortality of adult pigs as well as young pigs (Epperson et al., 1997). Therefore, it is even more important to control the spread of these new strains. Clinical signs vary between herds, indicating that viruses may differ in pathogenicity. Furthermore, effective use of vaccines and management approaches to control PRRS has not always been successful

    Micron-sized atom traps made from magneto-optical thin films

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    We have produced magnetic patterns suitable for trapping and manipulating neutral atoms on a 1μ1 \mum length scale. The required patterns are made in Co/Pt thin films on a silicon substrate, using the heat from a focussed laser beam to induce controlled domain reversal. In this way we draw lines and "paint" shaped areas of reversed magnetization with sub-micron resolution. These structures produce magnetic microtraps above the surface that are suitable for holding rubidium atoms with trap frequencies as high as ~1 MHz.Comment: 6 pages, 7 figure

    Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum)

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    The ability of plants to compete effectively for nitrogen (N) resources is critical to plant survival. However, controversy surrounds the importance of organic and inorganic sources of N in plant nutrition because of our poor ability to visualize and understand processes happening at the root�microbial�soil interface. Using high-resolution nano-scale secondary ion mass spectrometry stable isotope imaging (NanoSIMS-SII), we quantified the fate of 15N over both space and time within the rhizosphere. We pulse-labelled the soil surrounding wheat (Triticum aestivum) roots with either inline image or 15N-glutamate and traced the movement of 15N over 24 h. Imaging revealed that glutamate was rapidly depleted from the rhizosphere and that most 15N was captured by rhizobacteria, leading to very high 15N microbial enrichment. After microbial capture, approximately half of the 15N-glutamate was rapidly mineralized, leading to the excretion of inline image, which became available for plant capture. Roots proved to be poor competitors for 15N-glutamate and took up N mainly as inline image. Spatial mapping of 15N revealed differential patterns of 15N uptake within bacteria and the rapid uptake and redistribution of 15N within roots. In conclusion, we demonstrate the rapid cycling and transformation of N at the soil�root interface and that wheat capture of organic N is low in comparison to inorganic N under the conditions tested

    016 IL-1 SIGNALS BEYOND THE CANONICAL PATHWAY IN MURINE OA

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    Urea recycling in beef cattle fed prairie hay- based diets

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    Maximizing utilization of native rangeland is an important aspect of the cow/calf phase of beef production. Native rangeland is often of poor quality (less than 7% crude protein). Protein content of the rangeland is important because nitrogen is a key growth factor used by ruminal microbes. Without adequate nitrogen, the ruminal ecosystem will not operate at peak efficiency, which subsequently reduces the supply of nutrients to the animal. Historically, producers have provided supplemental nutrients to their cattle to achieve maximum performance. Both supplemental protein and energy have been provided to cattle consuming low-quality forage with varying levels of success. Typically, supplemental energy without adequate protein reduces fiber digestion by cattle. On the other hand, supplemental protein consistently improves overall performance

    Tachyon Tunnelling in D-brane-anti-D-brane

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    Using the tachyon DBI action proposal for the effective theory of non-coincident Dp_p-brane-anti-Dp_p-brane system, we study the decay of this system in the tachyon channel. We assume that the branes separation is held fixed, i.e. no throat formation, and then find the bounce solution which describe the decay of the system from false to the true vacuum of the tachyon potential. We shall show that due to the non-standard form of the kinetic term in the effective action, the thin wall approximation for calculating the bubble nucleation rate gives a result which is independent of the branes separation. This unusual result might indicate that the true decay of this metastable system should be via a solution that represents a throat formation as well as the tachyon tunneling.Comment: 15 pages, 4 figures, Latex file, minor changes, reference adde
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