Transgenic labeling of parvalbumin-expressing neurons with tdTomato

Parvalbumin (PVALB)-expressing fast-spiking interneurons subserve important roles in many brain regions by modulating circuit function and dysfunction of these neurons is strongly implicated in neuropsychiatric disorders including schizophrenia and autism. To facilitate the study of PVALB neuron function we need to be able to identify PVALB neurons in vivo. We have generated a bacterial artificial chromosome (BAC) transgenic mouse line expressing the red fluorophore tdTomato under the control of endogenous regulatory elements of the Pvalb gene locus (JAX # 027395). We show that the tdTomato transgene is faithfully expressed relative to endogenous PVALB expression throughout the brain. Furthermore, targeted patch clamp recordings confirm that the labeled populations in neocortex, striatum, and hippocampus are fast-spiking interneurons based on intrinsic properties. This new transgenic mouse line provides a useful tool to study PVALB neuron function in the normal brain as well as in mouse models of psychiatric disease.National Institute of Mental Health (U.S.) (Grant 5R01MH097104

HUMAN-CENTERED DESIGN OF THE HUMAN-SYSTEM INTERFACES OF MEDICAL EQUIPMENT: THYROID UPTAKE SYSTEM

Technology plays an important role in modern medical centers, making healthcare increasingly complex, relying on complex technical equipment. This technical complexity is particularly noticeable in the nuclear medicine. Poorly design human–system interfaces can increase the risks for human error. The human-centered approach emphasizes the development of the equipment with a deep understanding of the users activities, current work practices, needs and abilities of the users. An important concept of human-centered design is that the ease-of-use of the equipment can be ensured only if users are actively incorporated in all phases of the life cycle of design process. Representative groups of users are exposed to the equipment at various stages in development, in a variety of testing, evaluation and interviewing situations. The users feedback obtained is then used to refine the design, with the result serving as input to the next interaction of design process. The limits of the approach are that the users cannot address any particular future needs without prior experience or knowledge about the equipment operation. The aim of this paper is to present a methodological framework that contributes to the design of the human-system interfaces, through an approach related to the users and their activities. A case study is described in which the methodological framework is being applied in development of new human-system interfaces of the thyroid uptake system

Analysis of a Homomorphic MAC-based scheme against tag pollution in RLNC-enabled wireless networks

Network Coding-enabled wireless networks are vulnerable to data pollution attacks where adversary nodes inject into the network polluted (i.e. corrupted) packets that prevent the destination nodes from decoding correctly. Even a small proportion of pollution can quickly propagate into other packets via re-coding, occurred at the intermediate nodes, and lead to resource waste. Therefore, during the past few years, several solutions have been proposed to provide resistance against data pollution attacks. One of the most well-known solutions is Homomorphic Message Authentication Code (HMAC). However, HMAC is susceptible to a new type of pollution attacks, called tag pollution attacks, in which a malicious node randomly modifies MAC tags appended at the end of the transmitted packets. To address this issue, we have recently proposed an HMAC-based scheme making use of two types of MAC tags to provide resistance against both data pollution attacks and tag pollution attacks. In this paper, we steer our focus on improving the resistance of our proposed scheme against tag pollution attacks by decreasing the number of MACs. Finally, we analyze the impact of the total number of MACs on the bandwidth overhead of the proposed scheme

Alcohol marketing in the Americas and Spain during the 2014 FIFA World Cup Tournament

Background and aims To identify the nature of visual alcohol references in alcohol advertisements during televised broadcasts of the 2014 FIFA World Cup Tournament matches and to evaluate cross-national differences according to alcohol marketing policy restrictiveness. Design Content analysis using the Delphi method and identification of in-game sponsorships. Setting Television broadcasts of the 2014 FIFA World Cup in Argentina, Brazil, Canada, Finland, France, Mexico, Spain and the United States. Cases Eighty-seven alcohol advertisements20 matches. Measurements Quantitative rating scales, combined with the Delphi rating technique, were used to determine compliance of the alcohol advertisements with the International Alliance for Responsible Drinking's (IARD) Guiding Principles. Recordings of five matches from four countries were also used to identify the number of in-and out-of-game alcohol brand appearances. Findings A total of 86.2% of all unique alcohol advertisements contained at least one violation of IARD's Guiding Principles, with violation rates ranging from 72.7% (Mexico) to 100% (Brazil). Countries with the least restrictive marketing policies had a higher prevalence of violations in guidelines designed to protect minors. There were 2.76 in-game alcohol brand appearances and 0.83 out-of-game alcohol brand appearances per minute. Brand appearances did not differ across countries or according to a country's marketing policy restrictiveness. Conclusions Self-regulation and statutory policies were ineffective at limiting alcohol advertising during the 2014 FIFA World Cup Tournament television broadcasts. Most advertisements contained content that violated the self-regulation codes, and there were high levels of within broadcast brand appearances.Pan American Health OrganizationAlcohol Research UKInstitute of Alcohol StudiesUniv Connecticut, Sch Med, Dept Community Med & Hlth Care, Farmington, CT USAUniv Fed Sao Paulo, Dept Psychiat, Sao Paulo, BrazilPan Amer Hlth Org, Alcohol & Subst Abuse, Washington, DC USADepartment of Psychiatry, Universidade Federal de São Paulo (UNIFESP), São Paulo, BrazilPAHO: SC-14-02239Web of Scienc

Evolutionary biology for the 21st century

New theoretical and conceptual frameworks are required for evolutionary biology to capitalize on the wealth of data now becoming available from the study of genomes, phenotypes, and organisms - including humans - in their natural environments.Molecular and Cellular BiologyOrganismic and Evolutionary Biolog

CAN LANDSCAPE UNITS MAP HELP THE CONSERVATION OF SPIX´S MACAW?

Several studies suggest that Cyanopsitta spixii occurs mainly in Tabebuia caraiba (Mart.) Bureau gallery woodlands of northern Bahia (Brazil). The recently creation of two Protected Areas (2018) is combining efforts to ensure the reintroduction and conservation of this Critically Endangered (Possibly Extinct in the Wild) macaw. Achieving species conservation requires the definition of priority conservation areas and habitat recovery and the Landscape Cartography can be a relevant tool for this purpose. This research aimed to verify if there is a correlation between Landscape Units map and the Spix’s macaw occurrence. Here the Protected Areas landscape is described in three main components: land cover, landforms and superficial geology and then overlapped to occurrence map of Spix’s macaw and its most similar syntopic parrot, Primolius maracana. Spatial correlation revealed a correspondence of 85.48% to Dry River Environment Landscape pattern and C. spixii occurrence, indicating the importance to prioritize fluvial Landscapes conservation, combined with conservation strategies that must include the local population, since this are important territories for their survival

Analysis of a Novel SPS Configuration Enabled by Lunar ISRU

Architectures for space-based solar power using in situ resource utilization (ISRU) of space materials can greatly reduce earth launch mass and can enable geometric capacity growth. These two factors allow the potential for low cost power generation after development of an in-space infrastructure. A collection of extraction and processing methods designed for lunar operation provides for large volumes of low cost solar panels. With abundant panels a novel configuration for solar power satellites (SPS) is possible which avoids many of the challenges of existing designs. The so-called "tin can" SPS has no moving parts. It includes integral thermal radiators. Station-keeping requirements are minimal. Structural integrity is designed-in so that balance of plant mass is minimal. In this work the architecture and infrastructure supporting the tin can SPS is developed to support rapid construction and deployment. Performance estimates for the SPS are provided regarding heat and energy balance, and specific mass requirements

Online Data-Driven Safety Certification for Systems Subject to Unknown Disturbances

Deploying autonomous systems in safety critical settings necessitates methods to verify their safety properties. This is challenging because real-world systems may be subject to disturbances that affect their performance, but are unknown a priori. This work develops a safety-verification strategy wherein data is collected online and incorporated into a reachability analysis approach to check in real-time that the system avoids dangerous regions of the state space. Specifically, we employ an optimization-based moving horizon estimator (MHE) to characterize the disturbance affecting the system, which is incorporated into an online reachability calculation. Reachable sets are calculated using a computational graph analysis tool to predict the possible future states of the system and verify that they satisfy safety constraints. We include theoretical arguments proving our approach generates reachable sets that bound the future states of the system, as well as numerical results demonstrating how it can be used for safety verification. Finally, we present results from hardware experiments demonstrating our approach's ability to perform online reachability calculations for an unmanned surface vehicle subject to currents and actuator failures.Comment: 6 pages, 7 figure