Role of the church in reaching next generation : envisioning Bangalore churches on the significance of childen\u27s ministry in North India : to maximize their support

https://place.asburyseminary.edu/ecommonsatsdissertations/2238/thumbnail.jp

Lipase induction in Yarrowia lipolytica for castor oil hydrolysis and its effect on γ-decalactone production

γ-Decalactone is an aromatic compound of industrial interest, resulting from the biotransformation of ricinoleic acid, the major constituent of castor oil. In order to increase the availability of the substrate to the cells for the aroma production, castor oil previously hydrolyzed can be used. This hydrolysis may be promoted by enzymatic action, more specifically by lipases. In this work, the influence upon the aroma production of the lipase produced by Yarrowia lipolytica, a microorganism able to carry out the biotransformation, was studied. In a first approach, lipase induction conditions were analyzed using different Y. lipolytica strains and culture conditions, such as the inoculation mode of the lipase production medium. Lipase production was not affected by the cells centrifugation, so this step was eliminated, reducing the time and phases of the process. Moreover, Y. lipolytica W29 was shown to be the most adequate strain for lipase production. To investigate the importance of castor oil hydrolysis, the pre-addition of an inducer of lipase production (olive oil) to the biotransformation medium was tested. Results showed that the highest aroma production (1,600 mg L−1) was obtained without a lipase inducer. However, the pre-induction of lipase decreased the lag phase for γ-decalactone secretion.The authors acknowledge Fundacao para a Ciencia e Tecnologia (FCT) for the financial support provided (SFRH/BD/28039/2006 and SFRH/BD/63701/2009). They also acknowledge the kind supply of Yarrowia lipolytica IMUFRJ 50862 by Professor Alice Coelho from UFRJ, Brazil, and the supply of Y. lipolytica W29 by Prof. Yves Wache, ENSBANA, Dijon, France

Comparative production of green fluorescent protein under co-expression of bacterial hemoglobin in Escherichia coli W3110 using different culture scales

Production of green fluorescent protein (GFP) as a model foreign protein using different culture scales under co-expression of Vitreoscilla hemoglobin (VHb) in the industrial Escherichia coli strain W3110 (a K12 derivative), was examined. It was found that the VHb co-expressing W3110, exhibited an exceptional and sustained production ability during cell cultures using different scales, while the VHb non-expressing strain showed variable production levels. This high and sustained production ability indicates that the VHb co-expressing E. coli W3110, could be successfully employed for practical large-scale production cultures without the need for serious consideration of scale-up problems.X119sciescopuskc