Circadian Rhythms: Hijacking the Cyanobacterial Clock

SummaryUsing basic research to advance a practical application, a recent study demonstrates that the circadian clock in cyanobacteria can be ‘reprogrammed’ to improve yields of heterologous protein production — a green future surely beckons

Wave-number Selection by Target Patterns and Side Walls in Rayleigh-Benard Convection

We present experimental results for Rayleigh-Benard convection patterns in a cylindrical container with static side-wall forcing induced by a heater. This forcing stabilized a pattern of concentric rolls (a target pattern) with the central roll (the umbilicus) at the center of the cell after a jump from the conduction to the convection state. A quasi-static increase of the control parameter (epsilon) beyond 0.8 caused the umbilicus of the pattern to move off center. As observed by others, a further quasi-static increase of epsilon up to 15.6 caused a sequence of transitions. Each transition began with the displacement of the umbilicus and then proceeded with the loss of one convection roll at the umbilicus and the return of the umbilicus to a location near the center of the cell. Alternatively, with decreasing epsilon new rolls formed at the umbilicus but large umbilicus displacements did not occur. In addition to quantitative measurements of the umbilicus displacement, we determined and analyzed the entire wave-director field of each image. The wave numbers varied in the axial direction, with minima at the umbilicus and at the cell wall and a maximum at a radial position close to 2/3 Gamma. The wave numbers at the maximum showed hysteretic jumps at the transitions, but on average agreed well with the theoretical predictions for the wave numbers selected in the far field of an infinitely extended target pattern.Comment: ReVTeX, 11 pages, 16 eps figures include

A Systematic Search for X-ray Cavities in the Hot Gas of Galaxy Groups

We have performed a systematic search for X-ray cavities in the hot gas of 51 galaxy groups with Chandra archival data. The cavities are identified based on two methods: subtracting an elliptical beta model fitted to the X-ray surface brightness, and performing unsharp masking. 13 groups in the sample 25% are identified as clearly containing cavities, with another 13 systems showing tentative evidence for such structures. We find tight correlations between the radial and tangential radii of the cavities, and between their size and projected distance from the group center, in quantitative agreement with the case for more massive clusters. This suggests that similar physical processes are responsible for cavity evolution and disruption in systems covering a large range in total mass. We see no clear association between the detection of cavities and the current 1.4 GHz radio luminosity of the central brightest group galaxy, but there is a clear tendency for systems with a cool core to be more likely to harbor detectable cavities. To test the efficiency of the adopted cavity detection procedures, we employ a set of mock images designed to mimic typical Chandra data of our sample, and find that the model-fitting approach is generally more reliable than unsharp masking for recovering cavity properties. Finally, we find that the detectability of cavities is strongly influenced by a few factors, particularly the signal-to-noise ratio of the data, and that the real fraction of X-ray groups with prominent cavities could be substantially larger than the 25--50% suggested by our analysis.Comment: 19 pages, 16 figures, 2 tables, accepted by Ap

Photonic Sorting of Aligned, Crystalline Carbon Nanotube Textiles

Floating catalyst chemical vapor deposition uniquely generates aligned carbon nanotube (CNT) textiles with individual CNT lengths magnitudes longer than competing processes, though hindered by impurities and intrinsic/extrinsic defects. We present a photonic-based post-process, particularly suited for these textiles, that selectively removes defective CNTs and other carbons not forming a threshold thermal pathway. In this method, a large diameter laser beam rasters across the surface of a partly aligned CNT textile in air, suspended from its ends. This results in brilliant, localized oxidation, where remaining material is an optically transparent film comprised of few-walled CNTs with profound and unique improvement in microstructure alignment and crystallinity. Raman spectroscopy shows substantial D peak suppression while preserving radial breathing modes. This increases the undoped, specific electrical conductivity at least an order of magnitude to beyond that of single-crystal graphite. Cryogenic conductivity measurements indicate intrinsic transport enhancement, opposed to simply removing nonconductive carbons/residual catalyst

RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2

Genomic instability is a key hallmark of cancer leading to tumour heterogeneity and therapeutic resistance. ​BRCA2 has a fundamental role in error-free DNA repair but also sustains genome integrity by promoting ​RAD51 nucleofilament formation at stalled replication forks. ​CDK2 phosphorylates ​BRCA2 (pS3291-​BRCA2) to limit stabilizing contacts with polymerized ​RAD51; however, how replication stress modulates ​CDK2 activity and whether loss of pS3291-​BRCA2 regulation results in genomic instability of tumours are not known. Here we demonstrate that the Hippo pathway kinase ​LATS1 interacts with ​CDK2 in response to genotoxic stress to constrain pS3291-​BRCA2 and support ​RAD51 nucleofilaments, thereby maintaining genomic fidelity during replication stalling. We also show that ​LATS1 forms part of an ​ATR-mediated response to replication stress that requires the tumour suppressor ​RASSF1A. Importantly, perturbation of the ​ATR–​RASSF1A–​LATS1 signalling axis leads to genomic defects associated with loss of ​BRCA2 function and contributes to genomic instability and ‘BRCA-ness’ in lung cancers

Exclusive Hadronic Processes and Color Transparency

We review the current status of high energy exclusive processes and color transparency.Comment: 17 pages, 8 figures, based on talk given at International Symposium on Nuclear Physics, Mumbai, Dec 18-22, 200

Insights into toxic prymnesium parvum blooms:The role of sugars and algal viruses

Prymnesium parvum is a toxin-producing microalga that causes harmful algal blooms globally, which often result in large-scale fish kills that have severe ecological and economic implications. Although many toxins have previously been isolated from P. parvum, ambiguity still surrounds the responsible ichthyotoxins in P. parvum blooms and the biotic and abiotic factors that promote bloom toxicity. A major fish kill attributed to P. parvum occurred in Spring 2015 on the Norfolk Broads, a low-lying set of channels and lakes (Broads) found on the East of England. Here, we discuss how water samples taken during this bloom have led to diverse scientific advances ranging from toxin analysis to discovery of a new lytic virus of P. parvum, P. parvum DNA virus (PpDNAV-BW1). Taking recent literature into account, we propose key roles for sialic acids in this type of viral infection. Finally, we discuss recent practical detection and management strategies for controlling these devastating blooms

Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells

Temperature compensation and period determination by casein kinase 1 (CK1) are conserved features of eukaryotic circadian rhythms, whereas the clock gene transcription factors that facilitate daily gene expression rhythms differ between phylogenetic kingdoms. Human red blood cells (RBCs) exhibit temperature-compensated circadian rhythms, which, because RBCs lack nuclei, must occur in the absence of a circadian transcription-translation feedback loop. We tested whether period determination and temperature compensation are dependent on CKs in RBCs. As with nucleated cell types, broad-spectrum kinase inhibition with staurosporine lengthened the period of the RBC clock at 37°C, with more specific inhibition of CK1 and CK2 also eliciting robust changes in circadian period. Strikingly, inhibition of CK1 abolished temperature compensation and increased the Q10 for the period of oscillation in RBCs, similar to observations in nucleated cells. This indicates that CK1 activity is essential for circadian rhythms irrespective of the presence or absence of clock gene expression cycles