Presoaking and drying treatments to improve the performance of orchardgrass and bluegrass seed

The objective of this study was to develop a soaking and drying procedure to stimulate the germination and seedling emergence of orchardgrass (Dactylis glomerata L.) and Kentucky bluegrass (Poa pratensis L.) seed. Some of the physiological changes associated with the soaking and drying treatments were also studied. The seed was soaked on top of blotters moistened with solutions of 200 ppm GA₃, distilled H₂O and 20,000 ppm NaC1. The most beneficial results were obtained by soaking at 5 C for periods of 3 to 6 days. Following soaking, the seed lots were dried to their original dry weight and stored up to 8 months before testing. The effect of the treatment on the performance of the seed was evaluated in terms of germination and growth measurements. Presoaked seed germinated up to one day earlier than the unsoaked control. Radicle growth rate was not affected by any of the treatments. Seedling emergence from soil was more rapid after treatment. Germination tests conducted 8 months after presoaking indicated that the treatments had not caused any loss of viability during storage. Compared to water, there appeared to be little benefit from soaking in GA₃ and NaC1 solutions. Since seedling growth rates were not increased by the treatments, the beneficial results appeared to be related to the effects on germination rate. The small increase in imbibition rate and O₂ uptake by treated seeds may account for part of the accelerated germination. The treatments reduced the level of dormancy as indicated by the reduced requirement for alternating temperatures. Presoaking and drying treatments were most effective on cultivars and seed lots exhibiting higher levels of dormancy

A fundamental study of the mechanisms of action of polymers as retention and drainage aids. Project 3276, report three : a progress report to members of project 3276.

"April 4, 1977.""The Institute of Paper Chemistry, Robert A. Stratton, research associate, Norman L. Colson, research assistant, John W. Swanson, director, Division of Natural Materials and Systems.

Metabolic arsenal of giant viruses: host hijack or self-use?

© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Belhaouari, D., De Souza, G., Lamb, D., Kelly, S., Goldstone, J., Stegeman, J., Colson, P., La Scola, B., & Aherfi, S. Metabolic arsenal of giant viruses: host hijack or self-use? ELife, 11, (2022): e78674, https://doi.org/10.7554/elife.78674.Viruses generally are defined as lacking the fundamental properties of living organisms in that they do not harbor an energy metabolism system or protein synthesis machinery. However, the discovery of giant viruses of amoeba has fundamentally challenged this view because of their exceptional genome properties, particle sizes and encoding of the enzyme machinery for some steps of protein synthesis. Although giant viruses are not able to replicate autonomously and still require a host for their multiplication, numerous metabolic genes involved in energy production have been recently detected in giant virus genomes from many environments. These findings have further blurred the boundaries that separate viruses and living organisms. Herein, we summarize information concerning genes and proteins involved in cellular metabolic pathways and their orthologues that have, surprisingly, been discovered in giant viruses. The remarkable diversity of metabolic genes described in giant viruses include genes encoding enzymes involved in glycolysis, gluconeogenesis, tricarboxylic acid cycle, photosynthesis, and β-oxidation. These viral genes are thought to have been acquired from diverse biological sources through lateral gene transfer early in the evolution of Nucleo-Cytoplasmic Large DNA Viruses, or in some cases more recently. It was assumed that viruses are capable of hijacking host metabolic networks. But the giant virus auxiliary metabolic genes also may represent another form of host metabolism manipulation, by expanding the catalytic capabilities of the host cells especially in harsh environments, providing the infected host cells with a selective evolutionary advantage compared to non-infected cells and hence favoring the viral replication. However, the mechanism of these genes' functionality remains unclear to date.Royal Society - David C. Lamb Woods Hole Center for Oceans and Human Health - John J. Stegeman National Institutes of Health (P01ES021923) - John J. Stegeman National Science Foundation (OCE-1314642) - John J. Stegeman Agence Nationale de la Recherche ("Investments for the Future" program Méditerranée-Infection 10-IAHU-03) Djamal Brahim Belhaouari Gabriel Augusto Pires De Souza Philippe Colson Sarah Aherf

A case study in adaptable and reusable infrastructure at the Keck Observatory Archive: VO interfaces, moving targets, and more

The Keck Observatory Archive (KOA) (https://koa.ipac.caltech.edu) curates all observations acquired at the W. M. Keck Observatory (WMKO) since it began operations in 1994, including data from eight active instruments and two decommissioned instruments. The archive is a collaboration between WMKO and the NASA Exoplanet Science Institute (NExScI). Since its inception in 2004, the science information system used at KOA has adopted an architectural approach that emphasizes software re-use and adaptability. This paper describes how KOA is currently leveraging and extending open source software components to develop new services and to support delivery of a complete set of instrument metadata, which will enable more sophisticated and extensive queries than currently possible. In August 2015, KOA deployed a program interface to discover public data from all instruments equipped with an imaging mode. The interface complies with version 2 of the Simple Imaging Access Protocol (SIAP), under development by the International Virtual Observatory Alliance (IVOA), which defines a standard mechanism for discovering images through spatial queries. The heart of the KOA service is an R-tree-based, database-indexing mechanism prototyped by the Virtual Astronomical Observatory (VAO) and further developed by the Montage Image Mosaic project, designed to provide fast access to large imaging data sets as a first step in creating wide-area image mosaics (such as mosaics of subsets of the 4.7 million images of the SDSS DR9 release). The KOA service uses the results of the spatial R-tree search to create an SQLite data database for further relational filtering. The service uses a JSON configuration file to describe the association between instrument parameters and the service query parameters, and to make it applicable beyond the Keck instruments. The images generated at the Keck telescope usually do not encode the image footprints as WCS fields in the FITS file headers. Because SIAP searches are spatial, much of the effort in developing the program interface involved processing the instrument and telescope parameters to understand how accurately we can derive the WCS information for each instrument. This knowledge is now being fed back into the KOA databases as part of a program to include complete metadata information for all imaging observations. The R-tree program was itself extended to support temporal (in addition to spatial) indexing, in response to requests from the planetary science community for a search engine to discover observations of Solar System objects. With this 3D-indexing scheme, the service performs very fast time and spatial matches between the target ephemerides, obtained from the JPL SPICE service. Our experiments indicate these matches can be more than 100 times faster than when separating temporal and spatial searches. Images of the tracks of the moving targets, overlaid with the image footprints, are computed with a new command-line visualization tool, mViewer, released with the Montage distribution. The service is currently in test and will be released in late summer 2016

Hepatitis E Virus Seroprevalence and Chronic Infections in Patients with HIV, Switzerland

We screened 735 HIV-infected patients in Switzerland with unexplained alanine aminotransferase elevation for hepatitis E virus (HEV) immunoglobulin G. Although HEV seroprevalence in this population is low (2.6%), HEV RNA can persist in patients with low CD4 cell counts. Findings suggest chronic HEV infection should be considered as a cause of persistent alanine aminotransferase elevation

Intraoperative identification of esophageal sentinel lymph nodes with near-infrared fluorescence imaging

ObjectiveIn esophageal cancer, selective removal of involved lymph nodes could improve survival and limit complications from extended lymphadenectomy. Mapping with vital blue dyes or technetium Tc-99m often fails to identify intrathoracic sentinel lymph nodes. Our purpose was to develop an intraoperative method for identifying sentinel lymph nodes of the esophagus with high-sensitivity near-infrared fluorescence imaging.MethodsSix Yorkshire pigs underwent thoracotomy and received submucosal, esophageal injection of quantum dots, a novel near-infrared fluorescent lymph tracer designed for retention in sentinel lymph nodes. Six additional pigs underwent thoracotomy and received submucosal esophageal injection of CW800 conjugated to human serum albumin, another novel lymph tracer designed for uptake into distant lymph nodes. Finally, 6 pigs received submucosal injection of the fluorophore-conjugated albumin with an endoscopic needle through an esophagascope. These lymph tracers fluoresce in the near-infrared, permitting visualization of migration to sentinel lymph nodes with a custom intraoperative imaging system.ResultsInjection of the near-infrared fluorescent lymph tracers into the esophagus revealed communicating lymph nodes within 5 minutes of injection. In all 6 pigs that received quantum dot injection, only a single sentinel lymph node was identified. Among pigs that received fluorophore-conjugated albumin injection, in 5 of 12 a single sentinel lymph node was revealed, but in 7 of 12 two sentinel lymph nodes were identified. There was no dominant pattern in the appearance of the sentinel lymph nodes either cranial or caudal to the injection site.ConclusionNear-infrared fluorescence imaging of sentinel lymph nodes is a novel and reliable intraoperative technique with the power to assist with identification and resection of esophageal sentinel lymph nodes

Low Incidence of Chest Wall Pain with a Risk-Adapted Lung Stereotactic Body Radiation Therapy Approach Using Three or Five Fractions Based on Chest Wall Dosimetry

Purpose To examine the frequency and potential of dose-volume predictors for chest wall (CW) toxicity (pain and/or rib fracture) for patients receiving lung stereotactic body radiotherapy (SBRT) using treatment planning methods to minimize CW dose and a risk-adapted fractionation scheme. Methods: We reviewed data from 72 treatment plans, from 69 lung SBRT patients with at least one year of follow-up or CW toxicity, who were treated at our center between 2010 and 2013. Treatment plans were optimized to reduce CW dose and patients received a risk-adapted fractionation of 18 Gy×3 fractions (54 Gy total) if the CW V30 was less than 30 mL or 10–12 Gy×5 fractions (50–60 Gy total) otherwise. The association between CW toxicity and patient characteristics, treatment parameters and dose metrics, including biologically equivalent dose, were analyzed using logistic regression. Results: With a median follow-up of 20 months, 6 (8.3%) patients developed CW pain including three (4.2%) grade 1, two (2.8%) grade 2 and one (1.4%) grade 3. Five (6.9%) patients developed rib fractures, one of which was symptomatic. No significant associations between CW toxicity and patient and dosimetric variables were identified on univariate nor multivariate analysis. Conclusions: Optimization of treatment plans to reduce CW dose and a risk-adapted fractionation strategy of three or five fractions based on the CW V30 resulted in a low incidence of CW toxicity. Under these conditions, none of the patient characteristics or dose metrics we examined appeared to be predictive of CW pain

Potential Role of Aromatase over Estrogen Receptor Gene Polymorphisms in Migraine Susceptibility: A Case Control Study from North India

BACKGROUND: The present study was undertaken to find out the role of estrogen pathway related gene polymorphisms in susceptibility to migraine in Northern Indian population. Aromatase, CYP19A1 (rs10046 and rs4646); estrogen receptors, ESR1 (rs2234693, rs1801132, rs2228480 and rs9340799) and ESR2 (rs1271572 and rs1256049) polymorphisms were selected for the present study. METHODOLOGY/PRINCIPAL FINDINGS: The patients were recruited in two cohorts - primary (207) and replicative (127) along with 200 healthy controls and genotyped for various polymorphisms. Logistic regression analysis was applied for statistical analyses. The results were validated in the replicative cohort and pooled by meta analysis using Fisher's and Mantel-Haenszel test. Furthermore, Benjamini - Hochberg false discovery rate test was used to correct for multiple comparisons. CYP19A1 rs10046 and CYP19A1 rs4646 polymorphisms were found to confer risk and protective effect, respectively. Out of four ESR1 polymorphisms, only rs2234693 variant allele was significantly associated in migraine with aura. No significant associations were observed for ESR2 polymorphisms. Significant haplotypes were identified for CYP19A1 and ESR1 polymorphisms. Gene- gene interactions of genotypes as well as haplotypes were observed for CYP19A1- ESR1 showing both risk and protective combinations. CONCLUSION: We strongly suggest CYP19A1 polymorphisms to be the major contributing factors in migraine susceptibility instead of genetic variants of estrogen receptors

The curious role of sarcomeric proteins in control of diverse processes in cardiac myocytes

Introduction Relatively recent developments in our understanding of sarcomeric proteins have expanded their role from the home of molecular motors generating force and shortening to a cellular organelle fully integrated in the control of structural, electrical, mechanical, chemical, and metabolic homeostasis. Even so, in some cases these diverse functions of sarcomeric proteins appear to remain a curiosity, not fully appreciated in the analysis of major controllers of cardiac function. This attitude toward the function of sarcomeric proteins in cardiac myocytes is summarized in the following definition of “curiosity,” which seems particularly apropos: “meddlesome; thrusting oneself into and taking an active part in others’ affairs.” We focus in this Perspective on how sarcomeric proteins function in integration with membrane channels and transporters in control of cardiac dynamics, especially in adrenergic control of cardiac function. Understanding these mechanisms at the level of cardiac sarcomeres took on special significance with the identification of mutations in sarcomeric proteins as the most common cause of familial hypertrophic and dilated cardiomyopathies. These mutations commonly lead to structural, electrical, and metabolic remodeling and to sudden death. These disorders indicate a critical role of processes at the level of the sarcomeres in homeostatic control of cardiac energetics, dynamics, and structure. Yet, control of Ca2+ delivery to and removal from the myofilaments has dominated discussions of mechanisms regulating cardiac contractility. We first provide an alternative perspective in which rate processes at the level of the sarcomeres appear to be dominant during the rise and maintenance of systolic elastance and of isovolumic relaxation. A discussion of established adrenergic mechanisms and newly understood anti-adrenergic mechanisms controlling sarcomere response to Ca2+ follows and expands on this perspective

Antibiotic research and development: business as usual?

This article contends that poor economic incentives are an important reason for the lack of new drugs and explains how the DRIVE-AB intends to change the landscape by harnessing the expertise, motivation and diversity of its partner