9 research outputs found
Protocol for a randomised controlled trial of a family strengthening program to prevent unhealthy weight gain among 5 to 11-year-old children from at-risk families : the Strong Families Trial
Background: Obesity is an increasing health concern in Australia among adult and child populations alike and is often associated with other serious comorbidities. While the rise in the prevalence of childhood obesity has plateaued in high-income countries, it continues to increase among children from disadvantaged and culturally diverse backgrounds. The family environment of disadvantaged populations may increase the risk of childhood obesity through unhealthy eating and lifestyle practices. The Strong Families Trial aims to assess the effectiveness of a mixed behavioural and lifestyle intervention for parents and carers of at-risk populations, i.e. families from culturally diverse and disadvantaged backgrounds, in preventing unhealthy weight gain among children aged 5 to 11 years. Methods: Eight hundred families from low socio-economic areas in Greater Western Sydney, NSW, and Melbourne, VIC, will be recruited and randomised into a lifestyle intervention or control group. The intervention comprises 90-minute weekly sessions for 6 weeks (plus two-booster sessions) of an integrated, evidence-based, parenting and lifestyle program that accounts for the influences of family functioning. Primary (anthropometric data) and secondary (family functioning, feeding related parenting, physical activity, consumption of healthy foods, health literacy, family and household costs) outcome measures will be assessed at baseline, immediately following the intervention, and 12 months post-intervention. Discussion: This study will elucidate methods for engaging socially disadvantaged and culturally diverse groups in parenting programs concerned with child weight status. Trial Registration: This study is registered with the Australian New Zealand Clinical Trials Registry (ACTRN12619001019190). Registered 16 July 2019
Identifying essential factors for Oncogene-Induced Senescence using a novel cervical cancer cell culture model and high throughput screening approaches
Uterine Cervical Carcinoma (UCC) is the second most common cancer among women in the world and is induced by High risk Human Papillomaviruses (HPV). Cellular senescence is a potent tumor suppression mechanism that protects against cancer by triggering a proliferative arrest normally depending on the p53 and pRb pathways, but it can also promote aging and age-associated diseases. Because UCC cells express the HPV oncogenes E6 and E7 early in cancer progression, inactivating p53 and pRb, senescence is not expected to be functional in UCC. However, we recently found that the expression of oncogenic Ras in E6/E7-expressing primary keratinocytes strongly activated cellular senescence. Hypothesis: p53/pRb independent tumor suppression systems still exist in early UCC cells, and we can use our isogenic normal and HPV-E6/E7 expressing primary keratinocytes model to verify the role of the suspected TGFβ pathway or to identify novel p53/pRb-independent regulators of senescence in UCC. Approaches: I will use normal versus precancerous UCC human keratinocyte cell culture model to: 1. Directly test candidate genes like TGFβ that are potentially responsible for cellular senescence in precancerous UCC cells with inactive p53/pRb. 2. Apply an unbiased genome-wide genetic screen to identify new tumor suppressors that allow cellular senescence bypass in these cells. Conclusion et pertinence: Identified new targets/knowledge could be used to increase the efficacy of primary UCC treatments, lower the risk of recurrence, and to promote our understanding of normal human aging or other age-associated human diseases
Synthesis and anticancer activity evaluation of some hemin and hematoporphyrin derivatives
388-393Sulphamerazine, sulphadiazine, and
sulphaguanidine are coupled with hemin to give bis coupled products 1a, 1b
and 1c respectively. 3, 4-Diphenyliminothiazoline, sulphamerazine, sulphaacetamide,
sulphathiazole and sulphadiazine on coupling with hematoporphyrin give bis
coupled products 2a, 2b, 2c, 2d and 2e
respectively. Compounds 1a-c and 2a-e have
been screened for anticancer activity
against a small panel of six cancer cell lines consisting of prostate(DU 145),
colon (HT29), melanoma (LOX), breast(MCF7 and MCF7/ADR) and CNS(U251) tumors.
Best GI50 (concentration which inhibits the cell growth by 50%)
values are shown by 2c, 2.2μM(prostate tumor, cell line DU145);
2c 13.0μM(colon tumor, cell
line HT29); 2b, 3.4μM(melanoma
tumor, cell line LOX); 2c, 9.7μM(breast tumor, cell line MCF7); 2a,
3.1μM(breast, tumor, cell line MCF7/ADR) and 1c, 3.4μM(CNS
tumor, cell line U251) respectively. Out of all the compounds reported here GI50
value shown by 2a i.e.3.1μM against breast, tumor (MCF7/ADR) is quite
close to the GI50 value i.e. 1.8μM, of standard drug doxorubicin
Synthesis of biscoupled hemin-thiazoline derivatives and their anticancer activity evaluation
162-167A number of
biscoupled hemin-thiazoline derivatives 3a-i have been synthesized and
screened in vitro against six
human cancer cell lines consisting of lung large (NCIH460), colon
(HT29), breast (MCF7 and MCF7/ADR), prostate (DU 145) and CNS (U251) tumors.
Compound 3e exhibits good anticancer activity against lung large
(NCIH460; GI50 4.3MM), where compound 3g shows good anti
cancer activity against colon (HT29; GI50 0.9 μM), breast
(MCF7; GI50 0.5μM; MCF7/ADR; GI50 1.8μM),
prostate (DU 145; GI50 1.6μM) and CNS (U251; GI50 2.5μM)
tumors
Synthesis of sulpha drug acridine derivatives and their evaluation for anti-inflammatory, analgesic and anticancer activity
2659-2666Various sulpha drugs i.e. sulphaacetamide,
sulphathiazole, sulphadiazine and sulphamerazine are coupled with 9-chloro-2,4(un)substituted
acridines and 9- isothiocyanato-2, 4(un) substituted acridines to give corresponding
coupled products 3a-f and 4a-h respectively. The structures of all
synthesized compounds have been confirmed by spectroscopic methods.
Anti-inflammatory activity evaluation of 3a,b,c
and 4a-h was carried out and compounds 4a, 4d, 4g and
4h showed 8,13,22 and 3% activity respectively at 100mg/ kg p.o. Analgesic
activity evaluation of 3a,b,c and 4a-h indicated that these compounds
possess 25,75,50,25, 50,50, 0.75, 50, 50 and 50% analgesic activity at 100mg/kg
p.o. Anticancer activity
evaluation of 3a-f and 4a-h
against a small panel of seven cancer cell lines consisting of lung (NCIH 460);colon
(HT 29): melanoma (LOX); breast (MCF 7 and MCF 7 / ADR); prostate (DU 145) and
CNS (U251) tumors was carried out. Best GI50 (concentration which
inhibits the cell growth by 50%) values are shown by 4b, 0.4 μM (lung
carcinoma, cell line NCIH
460): 4b, 0.3 μM (colon tumor,
cell line HT29); 3e, 7.2μm (melanoma tumor, cell line
LOX): 4b, 0.7μM (breast tumor, cell line MCF7); 4c, 1.9μM (breast tumor, cell line MCF7/ADR); 4b, 0.8μM (prostate
tumor, cell line DU 145) and 4b, 1.4μ M (CNS tumor, cell line
U251) respectively. Out of all the compounds reported here GI50 value
shown by 4c i.e.
1.9μM against breast tumor
(MCF7/ADR) is quite close to the GI50 value i.e.1.2μM of the sta ndard drug doxorubicin. Also it is worthwhile
to mention here that compound 4b, has shown good anticancer activity against
four tumor cell lines i.e. GI50 value M.</i
Synthesis, hydrolysis over silica column, anticancer, anti-inflammatory and analgesic activity evaluation of some pyridine and pyrazine derivatives
387-399Various 3,4-diaryl-2-iminothiazolines 1a-s
have been condensed with 4-cyanopyridine and 2-cyanopyrazine by refluxing in
methanol for about 16 hr to give corresponding 3,4-diaryl-2-imino-N-(4'-pyridyliminomethyl)-4-thiazoline (2a-k, n-p) and 3,
4-diaryl-2-imino-N-(2'-pyrazinyliminomethyl)-4-thiazoline
(3a-m, q-s) derivatives. In some cases when these pyridyl and pyrazinyl
derivatives are purified by column chromatography over silica gel these compounds
get hydrolysed to give corresponding 3,4-diaryl-2-imino-N-(4'-carbonylpyridyl)-4-thiazoline (2o,p) and
3,4-diaryl-2-imino-N-(2'-carbonylpyrazinyl)-4-thiazoline
(3q-s) derivatives. The structures of all synthesized compounds have
been confirmed by spectroscopic methods. Compounds 2a-c,e-h,k,n,p, 3a-i
and 3l,m,q are screened for anticancer activity against a small panel of
six human cancer cell lines consisting of prostate (DU 145) colon (HT 29)
breast (MCF 7) breast (MCF 7/ADR), CNS (U 251) and lung large (NCIH 460)
tumors. Best GI50 values are shown by 3f, 11.5 M (prostate tumor, cell line DU 145), 3f, 1.0 M (colon tumor, cell line HT 29), 2n, 6.2M (breast tumor, cell line MCF 7), 2p, 4.8M (breast tumor, cell line MCF 7/ADR), 2p, 6.3M (CNS tumor, cell line U 251) and 3f, 0.9 M (lung large carcinoma, cell line NCIH 460) respectively. Compound 3f
has shown good anticancer activity against three cancer cell lines, whereas
compounds 2n and 2p against one and two cancer cell lines
respectively. Antiinflammatory
activity evaluation of 2a-k,n,o,p, 3a-m and 3q,r,s has
been carried out and compounds 2a-h, 2j, 2o,p, 3a,b,c,g,m and 3r showed
13, 32.5, 48.8, 6.5, 13.9, 7.0, 4.3, 16.6, 20.0, 17.3, 27.7, 16.2, 18.4, 34.7,
15.6, 24.0 and 4.7% activity, respectively, at 100mg/kg p.o. Analgesic
activity, evaluation of
2a-k,n,o,p, 3a-m and 3q,r,s indicates that these compounds
possess 50, 25,75, 25, 50, 75, 50, 50, 25, 0.0, 50, 50, 0.0, 75, 50, 25, 25,
25, 75, 0.0, 25, 25, 25, 50, 50, 75, 25, 50, 75 and 50% analgesic activity at
100mg/kg p.o