A prospective cohort study in patients with type 2 diabetes mellitus for validation of biomarkers (PROVALID) –study design and baseline characteristics

Background/Aims: The prevalence of diabetes mellitus type 2 and kidney disease in these patients varies widely between European countries. Methods: In addition to store biosamples the “Prospective cohort study in patients with type 2 diabetes mellitus for validation of biomarkers” collects information on history, physical status, laboratory measurements and medication in 4000 patients with diabetes mellitus type 2, being taken care of at the primary level of healthcare in 5 European countries (Austria, Hungary, Netherlands, Poland and Scotland). Next to comparing the rate of loss of eGFR between the countries, a further objective of the PROVALID study is to determine the 5-year cumulative incidence of renal and cardiovascular outcomes. Results: The mean age of the population recruited is 62.9±10 years, 54.6% are male and the mean BMI is 30.9±5.4 kg/m2 . Metabolic control (median HBA1c 6.8 % (6.2;7.5)) is achieved via administration of metformin in 67.4% of the patients and insulin in 30.3%. Median systolic and diastolic blood pressure at recruitment is 135 (125;146) and 80 (72;85) mmHg, 65.4% of subjects received RAAS blocking agents. Mean eGFR is 80.7±29.2 ml/min/1.73m2 and median baseline albumin/creatinine ratio 8.3 mg (IQR: 3.8 and 25.1). Conclusion: PROVALID will provide information on incidence and progression of renal and cardiovascular disease and therapy in patients with type 2 diabetes mellitus in different European countries. Thus, in contrast to many other cohort studies we will be able to associate national clinical practise pattern with outcome in this highly vulnerable patient population

Dislocation-mediated and twinning-induced plasticity of CoCrFeMnNi in varying tribological loading scenarios

Coarse-grained, metallic materials undergo microstructure refinement during tribological loading. This in turn results in changing tribological properties, so understanding deformation under tribological load is mandatory when designing tribological systems. Single-trace experiments were conducted to understand the initiation of deformation mechanisms acting in various tribological systems. The main scope of this work was to investigate the influence of normal and friction forces as well as crystal orientations on the dominating deformation mechanism in a face-centred cubic concentrated solid solution. While varying the normal force is easily realised, varying friction forces were achieved by using several counter body materials paired against CoCrFeMnNi. The subsurface deformation layer was either mediated through dislocation slip or twinning, depending on the grain orientation and on the tribological system. A layer dominated by dislocation-based deformation is characterised by lattice rotation, the formation of a dislocation trace line or subgrain formation. Such behaviour is observed for tribological systems with a low friction coefficient. For systems dominated by deformation twinning, three types of twin appearance were observed: small twins interacting with the surface, large twins and grains with two active twin systems. Two different twinning mechanisms are discussed as responsible for these characteristics

Handreichung: Forschungsdatenzentren gründen

Die Verbesserung des Zugangs zur Forschungsdaten ist derzeit ein zentrales wissenschaftspolitisches Thema. Forschungsdatenzentren (FDZ) haben sich in den vergangenen 20 Jahren als Modell für einen sicheren Datenzugang etabliert. In ihrer Datenstrategie sieht die Bundesregierung deshalb die Stärkung der bestehenden Forschungsdatenzentren und die Gründung neuer Forschungsdatenzentren vor. Diese Handreichung soll einen ersten Einblick in Aufgaben und Strukturen von Forschungsdatenzentren geben. Zwar unterscheiden sich Forschungsdatenzentren in Abhängigkeit von Umfang und Art des Datenangebotes und der Nutzungsfrequenz stark voneinander, bestimmte Aufgaben muss jedoch jedes Forschungsdatenzentrum erfüllen, um einen nutzungsfreundlichen Datenzugang anbieten zu können. Diese Handreichung fasst daher die Erfahrungen von 41 Forschungsdatenzentren zusammen, die sich seit 2001 durch den Rat für Sozial- und Wirtschaftsdaten haben akkreditieren lassen. Forschungsdatenzentren sind vor allem für die Einrichtung eines Zugangs zu schützenswerten Daten notwendig, andere Daten können auch über eines der zahlreichen Repositorien für Forschungsdaten veröffentlicht werden (vgl. Abschnitt 2). Allerdings ist die Bereitstellung von Daten immer mit der Notwendigkeit der Aufbereitung, Dokumentation und langfristigen Sicherung der Daten verbunden (vgl. Abschnitt 3). Dafür wird ausreichend und qualifiziertes Personal benötigt, das an der Schnittstelle zwischen Fachwissenschaft und Informationsinfrastrukturen arbeitet (vgl. Abschnitt 4). Die Leistungsfähigkeit des Forschungsdatenzentrums hängt entscheidend von der Qualität der IT-Infrastruktur ab (vgl. Abschnitt 5). Bei der Bereitstellung von schützenswerten Daten und im operativen Betrieb des Forschungsdatenzentrums müssen schließlich zahlreiche rechtliche Fragen berücksichtigt werden (vgl. Abschnitt 6). Die Handreichung schließt mit einem kurzen Einblick in den Prozess der Gründung eines Datenzentrums (vgl. Abschnitt 7) und die Akkreditierung durch den Rat für Sozial- und Wirtschaftsdaten (vgl. Abschnitt 8)

ERP hoch 3: Energieraumplanung entlang von ÖV-Achsen

Im Rahmen des national geförderten Forschungsprojekt „ERP_hoch3“ wird der Themenschwerpunkt Energieraumplanung in drei Fokusebenen betrachtet, untersucht und simuliert. Während „ERP“ für Energieraum-planung steht, steht die „3“ für drei verschiedene Raumbezüge – Stadtquartiere, öffentliche Verkehrsachsen und interkommunale Flächenpotenziale erneuerbarer Energien (Region). ERP_hoch3 ist ein zweijähriges Grundlagenforschungsprojekt, gefördert vom österreichischen Klimafonds. Das Forschungsteam besteht aus 14 Expertinnen und Experten der Fachbereiche für Regionalplanung und für örtliche Raumplanung (TU Wien, Department für Raumplanung) und der Institute für Städtebau und Prozess- und Partikeltechnik (TU Graz)

Mitochondrial Haplogroups, Control Region Polymorphisms and Malignant Melanoma: A Study in Middle European Caucasians

Because mitochondria play an essential role in energy metabolism, generation of reactive oxygen species (ROS), and apoptosis, sequence variation in the mitochondrial genome has been postulated to be a contributing factor to the etiology of multifactorial age-related diseases, including cancer. The aim of the present study was to compare the frequencies of mitochondrial DNA (mtDNA) haplogroups as well as control region (CR) polymorphisms of patients with malignant melanoma (n = 351) versus those of healthy controls (n = 1598) in Middle Europe.Using primer extension analysis and DNA sequencing, we identified all nine major European mitochondrial haplogroups and known CR polymorphisms. The frequencies of the major mitochondrial haplogroups did not differ significantly between patients and control subjects, whereas the frequencies of the one another linked CR polymorphisms A16183C, T16189C, C16192T, C16270T and T195C were significantly higher in patients with melanoma compared to the controls. Regarding clinical characteristics of the patient cohort, none of the nine major European haplogroups was associated with either Breslow thickness or distant metastasis. The CR polymorphisms A302CC-insertion and T310C-insertion were significantly associated with mean Breslow thickness, whereas the CR polymorphism T16519C was associated with metastasis.Our results suggest that mtDNA variations could be involved in melanoma etiology and pathogenesis, although the functional consequence of CR polymorphisms remains to be elucidated

Trace gas/aerosol boundary concentrations and their impacts on continental-scale AQMEII modeling domains

Copyright 2011 Elsevier B.V., All rights reserved.Over twenty modeling groups are participating in the Air Quality Model Evaluation International Initiative (AQMEII) in which a variety of mesoscale photochemical and aerosol air quality modeling systems are being applied to continental-scale domains in North America and Europe for 2006 full-year simulations for model inter-comparisons and evaluations. To better understand the reasons for differences in model results among these participating groups, each group was asked to use the same source of emissions and boundary concentration data for their simulations. This paper describes the development and application of the boundary concentration data for this AQMEII modeling exercise. The European project known as GEMS (Global and regional Earth-system Monitoring using Satellite and in-situ data) has produced global-scale re-analyses of air quality for several years, including 2006 (http://gems.ecmwf.int). The GEMS trace gas and aerosol data were made available at 3-hourly intervals on a regular latitude/longitude grid of approximately 1.9° resolution within 2 "cut-outs" from the global model domain. One cut-out was centered over North America and the other over Europe, covering sufficient spatial domain for each modeling group to extract the necessary time- and space-varying (horizontal and vertical) concentrations for their mesoscale model boundaries. Examples of the impact of these boundary concentrations on the AQMEII continental simulations are presented to quantify the sensitivity of the simulations to boundary concentrations. In addition, some participating groups were not able to use the GEMS data and instead relied upon other sources for their boundary concentration specifications. These are noted, and the contrasting impacts of other data sources for boundary data are presented. How one specifies four-dimensional boundary concentrations for mesoscale air quality simulations can have a profound impact on the model results, and hence, this aspect of data preparation must be performed with considerable care.Peer reviewedFinal Accepted Versio

The Mitochondrial T16189C Polymorphism Is Associated with Coronary Artery Disease in Middle European Populations

BACKGROUND: The pivotal role of mitochondria in energy production and free radical generation suggests that the mitochondrial genome could have an important influence on the expression of multifactorial age related diseases. Substitution of T to C at nucleotide position 16189 in the hypervariable D-loop of the control region (CR) of mitochondrial DNA (mtDNA) has attracted research interest because of its suspected association with various multifactorial diseases. The aim of the present study was to compare the frequency of this polymorphism in the CR of mtDNA in patients with coronary artery disease (CAD, n = 482) and type 2 diabetes mellitus (T2DM, n = 505) from two study centers, with healthy individuals (n = 1481) of Middle European descent in Austria. METHODOLOGY AND PRINCIPAL FINDINGS: CR polymorphisms and the nine major European haplogroups were identified by DNA sequencing and primer extension analysis, respectively. Frequencies and Odds Ratios for the association between cases and controls were calculated. Compared to healthy controls, the prevalence of T16189C was significantly higher in patients with CAD (11.8% vs 21.6%), as well as in patients with T2DM (11.8% vs 19.4%). The association of CAD, but not the one of T2DM, with T16189C remained highly significant after correction for age, sex and body mass index (BMI) and was independent of the two study centers. CONCLUSIONS AND SIGNIFICANCE: Our results show for the first time a significant association of T16189C with CAD in a Middle European population. As reported in other studies, in patients with T2DM an association with T16189C in individuals of European decent remains questionable