Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy

Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 mu m) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions

Mise en pratique for the definition of the candela and associated derived units for photometric and radiometric quantities in the International System of Units (SI)

The purpose of this mise en pratique, prepared by the Consultative Committee for Photometry and Radiometry (CCPR) of the International Committee for Weights and Measures (CIPM) and formally adopted by the CIPM, is to provide guidance on how the candela and related units used in photometry and radiometry can be realized in practice. The scope of the mise en pratique recognizes the fact that the two fields of photometry and radiometry and their units are closely related through the current definition of the SI base unit for the photometric quantity, luminous intensity: the candela. The previous version of the mise en pratique was applied only to the candela whereas this updated version covers the realization of the candela and other related units used for photometric and radiometric quantities. Recent advances in the generation and manipulation of individual photons show great promise of producing radiant fluxes with a well-established number of photons. Thus, this mise en pratique also includes information on the practical realization of units for photometric and radiometric quantities using photon-number-based techniques. In the following, for units used for photometric and radiometric quantities, the shorter term, photometric and radiometric units, is generally used. Section 1 describes the definition of the candela which introduces a close relationship between photometric and radiometric units. Sections 2 and 3 describe the practical realization of radiometric and photon-number-based units, respectively. Section 4.1 explains how, in general, photometric units are derived from radiometric units. Sections 4.2\u20134.5 deal with the particular geometric conditions for the specific photometric units. Section 5 deals very briefly with the topic of determination of measurement uncertainties in photometry.Peer reviewed: YesNRC publication: Ye

Mise en pratique for the definition of the candela and associated derived units for photometric and radiometric quantities in the International System of Units (SI)

open8The purpose of this mise en pratique, prepared by the Consultative Committee for Photometry and Radiometry (CCPR) of the International Committee for Weights and Measures (CIPM) and formally adopted by the CIPM, is to provide guidance on how the candela and related units used in photometry and radiometry can be realized in practice. The scope of the mise en pratique recognizes the fact that the two fields of photometry and radiometry and their units are closely related through the current definition of the SI base unit for the photometric quantity, luminous intensity: the candela. The previous version of the mise en pratique was applied only to the candela whereas this updated version covers the realization of the candela and other related units used for photometric and radiometric quantities. Recent advances in the generation and manipulation of individual photons show great promise of producing radiant fluxes with a well-established number of photons. Thus, this mise en pratique also includes information on the practical realization of units for photometric and radiometric quantities using photon-number-based techniques. In the following, for units used for photometric and radiometric quantities, the shorter term, photometric and radiometric units, is generally used. Section 1 describes the definition of the candela which introduces a close relationship between photometric and radiometric units. Sections 2 and 3 describe the practical realization of radiometric and photon-number-based units, respectively. Section 4.1 explains how, in general, photometric units are derived from radiometric units. Sections 4.2–4.5 deal with the particular geometric conditions for the specific photometric units. Section 5 deals very briefly with the topic of determination of measurement uncertainties in photometry.openZwinkels, Joanne; Sperling, Armin; Goodman, Teresa; Acosta, Joaquin Campos; Ohno, Yoshi; Rastello, Maria Luisa; Stock, Michael; Woolliams, EmmaZwinkels, Joanne; Sperling, Armin; Goodman, Teresa; Acosta, Joaquin Campos; Ohno, Yoshi; Rastello, Maria Luisa; Stock, Michael; Woolliams, Emm

Assessing resilience against floods with a system dynamics approach: a comparative study of two models

Purpose This paper aims to present the concepts of two different ways of generating a dynamic structure of the urban system to further allow in understanding specific urban behavior facing against flood and further evaluate the potential effect of specific resilience strategies aiming to decrease the exposure and vulnerability of the system. Design/methodology/approach Two system dynamics model structures are presented in form of Casual Loop Diagrams. Findings The main differences among the tow approaches are the time horizon and the approach that regulates the assessment of the resilience through a dynamic composite indicator: the first model refers to baseline at initial simulation time; the second model is focused on the ratio service supply to demand. Research limitations/implications Within the approach, the purpose is to properly and efficiently evaluate the effect of different Flood Risk Management strategies, i.e. prevention, defence, mitigation, preparation and recovery for consistent and resilient flood governance plans with different type of resilience scenarios. Originality/value The need for such tool is underlined by a lack on the assessment of urban resilience to flood as whole, considering the physical and social dimensions and the complex interaction among their main components. There are several assessment tools based on an indicator approach that have been proposed to meet this need. Nevertheless, indicator-based approach has the limitation to exclude the complexity of the system and its systemic interaction in terms of feedbacks’ effects among the identified components or variables selected for the system description. This peculiarity can be provided by System Dynamics modeling

Implementación de conceptos y herramientas de la filosofía Lean Construction en las empresas constructoras de la Ciudad de Cochabamba – Bolivia

The objective of this study is to identify the strategies used by the construction companies of the city of Cochabamba-Bolivia in the execution of projects, considering the tools and concepts of Lean Construction. The methodology consisted of a survey of 63 companies in the city of Cochabamba-Bolivia through a questionnaire made up of 44 questions. Four aspects were consulted: a) profile of project managers, b) general company information, c) planning and execution of projects and d) knowledge of the Lean Construction philosophy. The results show that companies recognize the problems in productivity and generation of waste, but do not carry out specific solutions. On the other hand, companies have listened to and even implemented some Lean Construction tools; however, the majority (70%) do not know the philosophy. Although barriers to implementing the philosophy are identified, actions that can collaborate in its application are also highlighted, such as demonstrating results with benefits and training staffEl objetivo del presente estudio es identificar las estrategias utilizadas por las empresas constructoras de la ciudad de Cochabamba-Bolivia en la ejecución de proyectos, considerando las herramientas y conceptos de Lean Construction. La metodología consistió en una encuesta a 63 empresas de la ciudad de Cochabamba-Bolivia mediante un cuestionario conformado por 44 preguntas. Se consultaron cuatro aspectos: a) perfil de los encargados de proyectos, b) información general de la empresa, c) planificación y ejecución de proyectos y d) conocimiento de la filosofía Lean Construction. Los resultados muestran que las empresas reconocen los problemas en la productividad y generación de desperdicios, pero no realizan soluciones específicas. Por otro lado, las empresas han escuchado e incluso implementando algunas herramientas de Lean Construction; sin embargo, la mayoría (70%) no conoce la filosofía. Si bien se identifican barreras para implementar la filosofía, también se resaltan acciones que pueden colaborar en su aplicación, tales como demostrar resultados con utilidades y capacitar al personal

Traumatic subarachnoid pleural fistula in children: Case report, algorithm and classification proposal

Subarachnoid pleural fistulas are rare. They have been described as complications of thoracic surgery, penetrating injuries and spinal surgery, among others. We present the case of a 3-year-old female child, who suffer spinal cord trauma secondary to a car accident, developing a posterior subarachnoid pleural fistula. To our knowledge this is the first reported case of a pediatric patient with subarachnoid pleural fistula resulting from closed trauma, requiring intensive multimodal management. We also present a management algorithm and a proposed classification. The diagnosis of this pathology is difficult when not associated with neurological deficit. A high degree of suspicion, multidisciplinary management and timely surgical intervention allow optimal management

Variants in toll-like receptor 9 gene influence susceptibility to tuberculosis in a Mexican population

Background: The control of Mycobacterium tuberculosis (Mtb) infection begins with the recognition of mycobacterial structural components by toll like receptors (TLRs) and other pattern recognition receptors. Our objective was to determine the influence of TLRs polymorphisms in the susceptibility to develop tuberculosis (TB) in Amerindian individuals from a rural area of Oaxaca, Mexico with high TB incidence. Methods: We carried out a case–control association community based study, genotyping 12 polymorphisms of TLR2, TLR4, TLR6 and TLR9 genes in 90 patients with confirmed pulmonary TB and 90 unrelated exposed but asymptomatic household contacts. Results: We found a significant increase in the frequency of the allele A of the TLR9 gene polymorphism rs352139 (A>G) in the group of TB patients (g.f. = 0.522) when compared with controls (g.f. = 0.383), (Pcorr = 0.01, OR = 1.75). Under the recessive model (A/G + A/A vs G/G) this polymorphism was also significantly associated with TB (Pcorr = 0.01, OR= 2.37). The association of the SNP rs352139 was statistically significant after adjustment by age, gender and comorbidities by regression logistic analysis (Dominant model: p value = 0.016, OR = 2.31; Additive model: p value = 0.023, OR = 1.68). The haplotype GAA of TLR9 SNPs was also associated with TB susceptibility (Pcorr = 0.02). Differences in the genotype or allele frequencies of TLR2, TLR4 and TLR6 polymorphisms between TB patients and healthy contacts were not detected. Conclusions: Our study suggests that the allele A of the intronic polymorphism rs352139 on TLR9 gene might contribute to the risk of developing TB in Mexican Amerindians

Frequency and Characteristics of familial melanoma in Spain: the FAM-GEM-1 Study.

Familial history of melanoma is a well-known risk factor for the disease, and 7% melanoma patients were reported to have a family history of melanoma. Data relating to the frequency and clinical and pathological characteristics of both familial and non-familial melanoma in Spain have been published, but these only include patients from specific areas of Spain and do not represent the data for the whole of Spain. PATIENTS AND METHODS: An observational study conducted by the Spanish Group of Melanoma (GEM) analyzed the family history of patients diagnosed with melanoma between 2011 and 2013 in the dermatology and oncology departments. RESULTS: In all, 1047 patients were analyzed, and 69 (6.6%) fulfilled criteria for classical familial melanoma (two or more first-degree relatives diagnosed with melanoma). Taking into account other risk factors for familial melanoma, such as multiple melanoma, pancreatic cancer in the family or second-degree relatives with melanoma, the number of patients fulfilling the criteria increased to 165 (15.8%). Using a univariate analysis, we determined that a Breslow index of less than 1 mm, negative mitosis, multiple melanoma, and a history of sunburns in childhood were more frequent in familial melanoma patients, but a multivariate analysis revealed no differences in any pathological or clinical factor between the two groups. CONCLUSIONS: Similar to that observed in other countries, familial melanoma accounts for 6.6% of melanoma diagnoses in Spain. Although no differences in the multivariate analysis were found, some better prognosis factors, such as Breslow index, seem more frequent in familial melanoma, which reflect a better early detection marker and/or a different biological behavior

Effect of incorrect use of dry powder inhalers on management of patients with asthma and COPD

SummaryBackgroundIncorrect usage of inhaler devices might have a major influence on the clinical effectiveness of the delivered drug. This issue is poorly addressed in management guidelines.MethodsThis article presents the results of a systematic literature review of studies evaluating incorrect use of established dry powder inhalers (DPIs) by patients with asthma or chronic obstructive pulmonary disease (COPD).ResultsOverall, we found that between 4% and 94% of patients, depending on the type of inhaler and method of assessment, do not use their inhalers correctly. The most common errors made included failure to exhale before actuation, failure to breath-hold after inhalation, incorrect positioning of the inhaler, incorrect rotation sequence, and failure to execute a forceful and deep inhalation. Inefficient DPI technique may lead to insufficient drug delivery and hence to insufficient lung deposition. As many as 25% of patients have never received verbal inhaler technique instruction, and for those that do, the quality and duration of instruction is not adequate and not reinforced by follow-up checks.ConclusionsThis review demonstrates that incorrect DPI technique with established DPIs is common among patients with asthma and COPD, and suggests that poor inhalation technique has detrimental consequences for clinical efficacy. Regular assessment and reinforcement of correct inhalation technique are considered by health professionals and caregivers to be an essential component of successful asthma management. Improvement of asthma and COPD management could be achieved by new DPIs that are easy to use correctly and are forgiving of poor inhalation technique, thus ensuring more successful drug delivery