Mandril de cateter na osteossíntese costal em um cão

O trauma torácico é uma afecção, comum na rotina de pequenos animais, sendo que muitos desses animais necessitam de abordagem cirúrgica e mergencial. O objetivo principal da estabilização torácica é permitir a adequada ventilação o mais cedo possível. Diante disso, o objetivo deste trabalho é relatar a aplicação de um mandril de cateter de acesso venoso, material de fácil acesso e baixo custo, na osteossíntese costal de um cão na rotina hospitalar. Um canino apresentando tórax paradoxal foi atendido no Hospital Veterinário da Universidade Federal de Santa Maria (HVU-UFSM). Foi realizada a osteossíntese da sexta e sétima costelas direitas, com fio mononáilon e com mandril de cateter, respectivamente. A técnica foi efetiva, ocorrendo a estabilização da parede costal no pós-operatório imediato e, após 60 dias, a fratura já havia consolidado. A utilização do mandril de cateter para a estabilização da fratura foi efetiva quando utilizada nesse caso da rotina cirúrgica. Mostrou-se um material de fácil manuseio e com uma maleabilidade adequada para sua introdução no canal medular da costela

Total fraction of mononuclear cell or stromal vascular fraction transplant associated with cellulosic membrane in experimental cutaneous wounds in rabbits

The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow (BM) or stromal vascular fraction (SVF) from adipose tissue (AT), associated with the use of cellulose membrane. To carry out this experiment, 20 rabbits were randomly divided in four groups with five animals each. After induction of the experimental cutaneous wound, Group A did not receive any treatment; group B received only cellulose membrane; and groups C and D, in addition to receiving the membrane, were submitted to autologous transplant of TMCF, with total cell value between 6,92 x 10(6) and 4,91 x 10(7) and a viability of 82 to 97% or SVF, with total cell value between 9,6 x 10(5) and 6,5 x 10(6) and a viability of 66 a 87%, respectively. At the end of the evaluation period, the three treated groups presented significant statistical difference of wound area in relation to the control group, and the group which received SVF from adipose tissue presented the shortest wound healing time.A pele apresenta diversas funções importantes para o organismo. A manutenção de sua integridade é fundamental, entre outras, para impedir a penetração de microrganismos e a perda de líquidos essenciais à manutenção da vida. Por estar constantemente exposta ao meio ambiente, a pele é altamente susceptível a traumas que podem acarretar soluções de continuidade. A cicatrização da pele ferida deve ser rápida e diversas alternativas são buscadas, visando à redução do tempo de reparo a fim de garantir cicatriz funcional e esteticamente aceitável. Uma opção cada vez mais viável para reparar tecidos danificados é a terapia celular com células-tronco adultas. Os objetivos deste trabalho consistiram em avaliar o transplante da fração total de células mononucleares (FTCM) da medula óssea (MO) e da fração vascular estromal (FVE) do tecido adiposo (TA), associado ao uso da membrana celulósica. Para realização deste experimento, 20 coelhos foram divididos aleatoriamente em quatro grupos com cinco animais cada. Após a indução da ferida cutânea experimental, o grupo A, não recebeu nenhum tratamento, o grupo B, recebeu apenas membrana celulósica e os grupos C e D, além de receberem a membrana, foram submetidos a transplante autólogo da FTCM, com valor total de células entre 6,92 x 10(6) a 4,91 x 10(7) e uma viabilidade de 82 a 97% ou da FVE, com valor total de células entre 9,6 x 10(5) e 6,5 x 10(6) e uma viabilidade de 66 a 87%, respectivamente. Ao final do período de avaliação, os três grupos tratados apresentaram diferença estatística significativa da área da ferida em relação ao grupo controle e o grupo que recebeu a FVE do tecido adiposo apresentou o menor tempo de cicatrização da ferida

Stem-Cell-Based Therapies for Improving Islet Transplantation Outcomes in Type 1 Diabetes

Beta-cell replacement by human islets or whole pancreas offers a life-saving therapeutic remedy for patients suffering from type 1 diabetes, providing considerable advantages with respect to diminishing total daily insulin dose and lowering frequencies of debilitating hypoglycemic reactions as well as preventing chronic micro- and macrovascular complications. Although remarkable progress has been made in this area, several hurdles remain, hampering its wide-spread applicability. Such hurdles include a limiting supply of islets, the necessity of several donors to achieve enough islet mass for insulin independence, and graft failure because of metabolic pressure, continued autoimmunity, alloimmunity, high concentrations of immunosuppressive drugs as well as oxidative stress caused by hypoxia or inflammation. On the other hand, the islet transplant procedure provides the possibility to undertake multiple practical and beneficial manipulations of the beta cells before engraftment with the intention to reach improved graft survival results. Here, we have focused on the current status of various obstacles in islet transplantation and on the potential of (stem)cell-based treatments able to stimulate islet graft outcome in pre-clinical and clinical transplantation settings in which specific attention is given to the engraftment-enhancing and immunomodulatory potential of various types of stem cells.status: publishe

Minimally invasive approach to renal artery using different types of catheter by conventional radiology

Background: Several routes of administration have been used for cell and gene therapy delivery in kidney disease in the last years. Moreover, a more homogenous distribution and a higher response was observed when administered intra-arterially. The main objective of the present study was to describe and to compare the use of Fogarty thru-lumen embolectomy catheter with angiographic catheter through the femoral artery by using conventional radiology as a guide. Materials, Methods & Results: Seven healthy dogs were used in these experiments. First, a Fogarty thru-lumen embolectomy catheter was introduced followed by an angiographic catheter. The primary outcome was the number of attempts for the placement of the introducer and the number of radiographic studies to carry out nephrography. Preceding the surgical procedure, ultrasonographic evaluation was performed in all animals in order to measure the diameter of the left and right femoral arteries, left and right renal and abdominal aorta at the renal level. After trichotomy and antisepsis of the medial face of the two pelvic limbs, the 6F valve introducer was placed based on the modified Seldinger technique Thereafter, aortography was performed, showing its collateral arteries, with contrast injection, and it was estimated how much of the Fogarty 5,5F catheter had to be introduced. Subsequently, a guidewire was inserted through the catheter followed by the Fogarty catheter. Another x-ray was taken to show the position of the catheter. It was observed whether the catheter was positioned correctly and the cuff was filled, and then another contrasting radiograph was performed. If the contrast was not going to the kidneys, the cuff of the catheter was deflated, the catheter was repositioned and a new contrast radiography was performed. For the angiographic catheters technique, it was estimated how much of the catheter needed to be introduced, and then the curvature of the catheter was directed to the left kidney, followed by placement of the guidewire. The guidewire was then removed and X-rays were taken to confirm the position as many times as necessary for the correct positioning of the catheter. After selective catheterization of the left kidney, the catheter was removed and its tip was repositioned for catheterization of the right kidney, repeating the same procedure After the radiographic evaluation, the 6F valve introducer was removed followed by the hemostasis of the femoral artery. In five out of seven animals it was possible to place the introducer tube 6F. Angiographic catheter technique needed significantly more radiographic films compared to Fogarty catheter when both arteries were evaluated. No significant difference was observed when compared to only one renal artery. The only complication observed was hematoma in three animals. Discussion: The access of the renal artery for drug delivery and cell therapy is possible using both Fogarty thru-lumen embolectomy catheter and angiographic catheter with conventional radiology as a guide in dogs over 10 kg. However, we considered the Fogarty catheter easier to be applied, since only one procedure is needed to access both renal arteries, thus making it less expensive and faster to run. If access to only one renal artery is required, the angiographic catheter is recommended, since cell therapy would be administered more selectively in the target kidney

Minimally Invasive Approach to Renal Artery Using Different Types of Catheter by Conventional Radiology

Background: Several routes of administration have been used for cell and gene therapy delivery in kidney disease in the last years. Moreover, a more homogenous distribution and a higher response was observed when administered intra-arterially. The main objective of the present study was to describe and to compare the use of Fogarty thru-lumen embolectomy catheter with angiographic catheter through the femoral artery by using conventional radiology as a guide.Materials, Methods & Results: Seven healthy dogs were used in these experiments. First, a Fogarty thru-lumen embolectomy catheter was introduced followed by an angiographic catheter. The primary outcome was the number of attempts for the placement of the introducer and the number of radiographic studies to carry out nephrography. Preceding the surgical procedure, ultrasonographic evaluation was performed in all animals in order to measure the diameter of the left and right femoral arteries, left and right renal and abdominal aorta at the renal level. After trichotomy and antisepsis of the medial face of the two pelvic limbs, the 6F valve introducer was placed based on the modified Seldinger technique. Thereafter, aortography was performed, showing its collateral arteries, with contrast injection, and it was estimated how much of the Fogarty 5,5F catheter had to be introduced. Subsequently, a guidewire was inserted through the catheter followed by the Fogarty catheter. Another x-ray was taken to show the position of the catheter. It was observed whether the catheter was positioned correctly and the cuff was filled, and then another contrasting radiograph was performed. If the contrast was not going to the kidneys, the cuff of the catheter was deflated, the catheter was repositioned and a new contrast radiography was performed. For the angiographic catheters technique, it was estimated how much of the catheter needed to be introduced, and then the curvature of the catheter was directed to the left kidney, followed by placement of the guidewire. The guidewire was then removed and X-rays were taken to confirm the position as many times as necessary for the correct positioning of the catheter. After selective catheterization of the left kidney, the catheter was removed and its tip was repositioned for catheterization of the right kidney, repeating the same procedure. After the radiographic evaluation, the 6F valve introducer was removed followed by the hemostasis of the femoral artery. In five out of seven animals it was possible to place the introducer tube 6F. Angiographic catheter technique needed significantly more radiographic films compared to Fogarty catheter when both arteries were evaluated. No significant difference was observed when compared to only one renal artery. The only complication observed was hematoma in three animals.Discussion: The access of the renal artery for drug delivery and cell therapy is possible using both Fogarty thru-lumen embolectomy catheter and angiographic catheter with conventional radiology as a guide in dogs over 10 kg. However, we considered the Fogarty catheter easier to be applied, since only one procedure is needed to access both renal arteries, thus making it less expensive and faster to run. If access to only one renal artery is required, the angiographic catheter is recommended, since cell therapy would be administered more selectively in the target kidney

Minimally invasive approach to renal artery using different types of catheter by conventional radiology

Background: Several routes of administration have been used for cell and gene therapy delivery in kidney disease in the last years. Moreover, a more homogenous distribution and a higher response was observed when administered intra-arterially. The main objective of the present study was to describe and to compare the use of Fogarty thru-lumen embolectomy catheter with angiographic catheter through the femoral artery by using conventional radiology as a guide. Materials, Methods & Results: Seven healthy dogs were used in these experiments. First, a Fogarty thru-lumen embolectomy catheter was introduced followed by an angiographic catheter. The primary outcome was the number of attempts for the placement of the introducer and the number of radiographic studies to carry out nephrography. Preceding the surgical procedure, ultrasonographic evaluation was performed in all animals in order to measure the diameter of the left and right femoral arteries, left and right renal and abdominal aorta at the renal level. After trichotomy and antisepsis of the medial face of the two pelvic limbs, the 6F valve introducer was placed based on the modified Seldinger technique Thereafter, aortography was performed, showing its collateral arteries, with contrast injection, and it was estimated how much of the Fogarty 5,5F catheter had to be introduced. Subsequently, a guidewire was inserted through the catheter followed by the Fogarty catheter. Another x-ray was taken to show the position of the catheter. It was observed whether the catheter was positioned correctly and the cuff was filled, and then another contrasting radiograph was performed. If the contrast was not going to the kidneys, the cuff of the catheter was deflated, the catheter was repositioned and a new contrast radiography was performed. For the angiographic catheters technique, it was estimated how much of the catheter needed to be introduced, and then the curvature of the catheter was directed to the left kidney, followed by placement of the guidewire. The guidewire was then removed and X-rays were taken to confirm the position as many times as necessary for the correct positioning of the catheter. After selective catheterization of the left kidney, the catheter was removed and its tip was repositioned for catheterization of the right kidney, repeating the same procedure After the radiographic evaluation, the 6F valve introducer was removed followed by the hemostasis of the femoral artery. In five out of seven animals it was possible to place the introducer tube 6F. Angiographic catheter technique needed significantly more radiographic films compared to Fogarty catheter when both arteries were evaluated. No significant difference was observed when compared to only one renal artery. The only complication observed was hematoma in three animals. Discussion: The access of the renal artery for drug delivery and cell therapy is possible using both Fogarty thru-lumen embolectomy catheter and angiographic catheter with conventional radiology as a guide in dogs over 10 kg. However, we considered the Fogarty catheter easier to be applied, since only one procedure is needed to access both renal arteries, thus making it less expensive and faster to run. If access to only one renal artery is required, the angiographic catheter is recommended, since cell therapy would be administered more selectively in the target kidney

Human multipotent adult progenitor cells enhance islet function and revascularisation when co-transplanted as a composite pellet in a mouse model of diabetes

Hypoxia in the initial days after islet transplantation leads to considerable loss of islet mass and contributes to disappointing outcomes in the clinical setting. The aim of the present study was to investigate whether co-transplantation of human non-endothelial bone marrow-derived multipotent adult progenitor cells (MAPCs), which are non-immunogenic and can secrete angiogenic growth factors during the initial days after implantation, could improve islet engraftment and survival.status: publishe